MAT 2 (0-1 cm). MAT 2 (1-2 cm). JX091072, (filament), Pfeffer et al., 2012. HG004419, Schauer et al., 2014. HG004410, (Single cable bacterium), Schauer et al., ...
Mats Crimea A
193
81 14
11
Crimea Mats B
Crimea mats C Y13672, Desulfocapsa sulfexigens 15 Crimea mats D AF118453, Desulforhopalus singaporensis Crimea mats Crimea mats Crimea mats Crimea mats E Crimea mats F AB110542, Desulfopila aestuarii
5
Crimea mats G
46
Crimea mats H
97
AM774321, Desulfopila inferna Crimea mats I
249
JX091073, (filament), Pfeffer et al., 2012
MAT 2 (0-1 cm) MAT 2 (1-2 cm)
JX091072, (filament), Pfeffer et al., 2012 HG004419, Schauer et al., 2014 HG004410, (Single cable bacterium), Schauer et al., 2014 HG004412, (Single cable bacterium), Schauer et al., 2014 HG004416, Schauer et al., 2014 HG004411, (Single cable bacterium), Schauer et al., 2014 HG004408, (Single cable bacterium), Schauer et al., 2014 HG004409, (Single cable bacterium), Schauer et al., 2014 HG004418, Schauer et al., 2014 HG004415, (Single cable bacterium), Schauer et al., 2014 HG004407, (Single cable bacterium), Schauer et al., 2014 HG004417, Schauer et al., 2014 HG004406, (Single cable bacterium), Schauer et al., 2014 HG004414, (Single cable bacterium), Schauer et al., 2014 HG004413, (Single cable bacterium), Schauer et al., 2014 HG004412, (Single cable bacterium), Schauer et al., 2014 HG004420, Schauer et al., 2014
MAT 2 (0-1 cm) MAT 2 (1-2 cm) MAT 2 (1-2 cm) MAT 4 (0-1 cm)
GU302481, Beggiatoa spp. mat, Lloyd et al., 2010 Crimea mats J HQ153922, White microbial film, shallow hydrothermal vent 10 Malkin et al., 2014 HQ153967, White microbial film, shallow hydrothermal vent HM750216, Desulfobulbus alkaliphilus AY548789, Desulfobulbus propionicus 2 Crimea mats K 4
26 22
0.10
Crimea mats L
Crimea mats M
HQ823668, Thiothrix nivea
Supplementary Figure 3. Phylogenetic 16S rRNA gene based tree showing the affiliation of sequences extracted from mat-covered sediments of the Crimea margin (“Crimea mats”) with sequences recovered from sediments and assigned to the filamentous “cable” bacteria known for long-range electron transport (Pfeffer et al., 2012; Malkin et al., 2014; Schauer et al., 2014). Mats and references sequences were screened for relatives to filamentous “cable” bacteria and extracted from the data set using the “grep” function in R. Initial tree reconstruction was conducted with nearly full-length sequences of Desulfobulbaceae (1200 - 1400 bp). Partial sequences (this study, Pfeffer et al, 2012, Malkin et al., 2014 and Schauer et al., 2014) were subsequently inserted into the reconstructed consensus tree by applying the parsimony criteria. Only selected sequences are shown. The scale bar corresponds to 10% estimated sequence divergence. Phylogenetic classification was carried out with the software package ARB (Ludwig et al., 2004) based on the SILVA database (SSURef v119, release date: 24 July 2014). Phylogenetic tree was calculated with the maximum likelihood algorithm PHYML (100 bootstraps) as implemented in ARB using a positional variability filter.
