Measurement of Midregional Proadrenomedullin ... - Semantic Scholar
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Measurement of Midregional Proadrenomedullin ... - Semantic Scholar
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Clinical Chemistry 51:10 1823–1829 (2005)
Endocrinology and Metabolism
Measurement of Midregional Proadrenomedullin in Plasma with an Immunoluminometric Assay Nils G. Morgenthaler,* Joachim Struck, Christine Alonso, and Andreas Bergmann Background: Adrenomedullin (ADM) is a potent vasodilatory peptide, and circulating concentrations have been described for several disease states, including dysfunction of the cardiovascular system and sepsis. Reliable quantification has been hampered by the short half-life, the existence of a binding protein, and physical properties. Here we report the technical evaluation of an assay for midregional pro-ADM (MR-proADM) that does not have these problems. Methods: MR-proADM was measured in a sandwich immunoluminometric assay using 2 polyclonal antibodies to amino acids 45–92 of proADM. The reference interval was defined in EDTA plasma of 264 healthy individuals (117 male, 147 female), and increased MRproADM concentrations were found in 95 patients with sepsis and 54 patients with cardiovascular disease. Results: The assay has an analytical detection limit of 0.08 nmol/L, and the interassay CV was 0.12 nmol/L. The assay was linear on dilution with undisturbed recovery of the analyte. EDTA-, heparin-, and citrate-plasma samples were stable (
