2014
Meetings and Courses
“IV ICGEB Workshop on Human RNA Viruses” EMBO Workshop jointly sponsored by ICGEB
Istanbul, Turkey 6 - 8 October 2014
http://www.icgeb.org/turkey-workshop-on-human-rna-viruses-2014.html Organisers: Önder Ergönül and Füsun Can (Koç University, Istanbul, Turkey) Co-organisers: Alessandro Marcello and Oscar Burrone (ICGEB, Trieste, Italy) Meeting Secretariat: ICGEB Conferences Unit
ICGEB
Padriciano, 99 34149 Trieste, Italy
Note: The Abstracts published in this meeting book should be treated as personal communications and be cited only with the consent of the author
Cover: Ultrastructural analysis of membrane alterations induced by tick-borne encephalitis infection (Miorin, L. et al, Journal of Virology 2013)
EMBO Workshop on Human RNA Viruses jointly sponsored by the ICGEB American Hospital, Istanbul (Turkey) 6-8 October 2014
ABSTRACTS BOOK
ABOUT THE WORKSHOP Viruses continue to evolve and emerge. Most viral diseases that pose epidemic threats are RNA viruses. This EMBO Workshop is co-sponsored and organized by the International Centre for Genetic Engineering and Biotechnology and is dedicated to bridging the gap between recent advances in molecular and cellular biology of human RNA viruses and the needs of those most affected by these diseases. It was launched in 2008 at ICGEB Trieste, Italy and is held every two years (New Delhi 2010, Buenos Aires 2012 - see the ICGEB web page dedicated to the event http://www.icgeb.org/turkey-workshop-on-human-rna-viruses-2014.html). The format traditionally provides an overview of several aspects concerning the biology of RNA viruses through state-of-the-art lectures from invited speakers, selected talks from participants and posters in an informal international environment. An international panel of speakers has been selected to provide an overview of the biology of the most relevant RNA virus infections of humans. Aspects of viral pathogenesis, together with deeper insights in the host response to viral infections, including innate immunity pathways and viral evasion strategies will be discussed at the venue. Novel technologies to explore in higher detail the intracellular behavior of RNA viruses will be also presented. Finally, the exploitation of results from basic science for viral detection, prevention and treatment will be considered. A number of selected talks and posters from participants, not necessarily limited to these topics, allows a lively discussion with the exchange of information and the establishment of scientific collaborations. THE ORGANIZING COMMITTEE Alessandro Marcello, Group Leader of Molecular Virology, ICGEB Trieste, ITALY. Oscar Burrone, Group Leader of Molecular Immunology, ICGEB, Trieste, ITALY. Önder Ergönül, Professor of Infectious Diseases and Clinical Microbiology, Koç University, School of Medicine, Istanbul, TURKEY. Füsun Can, Professor of Microbiology, Koç University, School of Medicine, Istanbul, TURKEY.
Website of the Workshop: http://events.embo.org/14-rna-viruses/index.html
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CONTACTS LIST INVITED SPEAKERS & ORGANIZERS Last name
First name
Email
Abacioglu Altan-Bonnet Arnoldi Burrone Can Enjuanes Ergönül Ergunay Fernandez-Sesma Fujita Gamarnik Garcia-Blanco Garcia-Sastre Horner Jameel Marcello McLauchlan Parahnos Baccala
Hakan Nihal Francesca Oscar Füsun Luis Önder Koray Ana Takashi Andrea Mariano Adolfo Stacy Shahid Alessandro John Glaucia
[email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected]
PRESENTING PARTICIPANTS Last name
First name
Email
Adegboyega Ali Alshuwaikh Bader El Din Bakhautdin Benitez Carletti Cesarec Cevik Chacon Chu Ciftci Cosset Dawood De Lorenzo Dreux Dufkova Edwards El Awady Elijah Kolawole Fajs Finol Berrueta Gamal García Gindeel Golkocheva-Markova
Aluko Muhammad Arwa Noha Esen Asiel Tea Mia Recep Emrah Luz Hyuk Cagla François-Loïc Reham Giuditta Marlène Lucie Megan Basma Oladipo Luka Esteban Andres Shiraz Cybele Nesrin Jaffar Hassan Elitsa
[email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected]
Goncharova Hamlin Huerta Ibrahim Jackson Jawhar Jouvenet Jurek Kassela Khan Kishta Kula Kumar Kurhade Lapazio Lopes Manel Marcotrigiano Milewska Mok Moroso Narkpuk Orozco-García Överby Oyediran Xavier Panganiban Saeed Santos-Mendoza Singh Širmarová Slon Campos Upadhyay Uranowska Vonderstein
Elena Rebecca Vivian Marwa William Gharbi Nolween Anna Katerina Rizma Sara Anna Binod Chaitanya Lucia Luciano Ben M'Hadheb Joseph Aleksandra Bobo Marie Jaraspim Elizabeth Anna Oyewale Maria Nito Mervat Teresa Abhishek Kumar Jana Jose Luis Arunkumar Karolina Kirstin
[email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected] [email protected]
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INVITED TALKS Hakan Abacioglu Dokuz Eylul University School of Medicine Balcova 35340 Izmir, Turkey “Molecular epidemiology of HCV in Turkey” Nihal Altan-Bonnet Laboratory of Host-Pathogen Dynamics Cell Biology and Physiology Center National Heart Lung and Blood Institute National Institutes of Health Bethesda MD 20892, USA “Non-enveloped viruses rely on phosphatidylserine-enriched vesicles for non-lytic release and subsequent new infections” Francesca Arnoldi University of Trieste, Italy “Rotavirus-host cell interactions” Oscar Burrone Laboratory of Molecular Immunology The International Centre for Genetic Engineering and Biotechnology, Trieste, Italy “Tricks on translation” Luis Enjuanes Department of Molecular and Cell Biology National Center of Biotechnology (CNB-CSIC), Madrid, Spain. “Human coronavirus pathogenesis and protection” Önder Ergönül Koç University, School of Medicine Intanbul, Turkey “Crimean-Congo Hemorrhagic Fever Virus” Koray Ergunay Department of Medical Microbiology, Virology Unit Hacettepe University Faculty of Medicine 06100 Sihhiye Ankara TURKEY “Newly-emergent Phleboviruses” Ana Fernandez-Sesma Icahn School of Medicine at Mount Sinai New York, NY, USA “Mechanisms of immune evasion by Dengue virus” Takashi Fujita Institute for Virus Research and Graduate School of Biostudies Kyoto University, Japan “Regulation of antiviral innate immunity by RIG-I-Like Receptors” Andrea Gamarnik Fundación Instituto Leloir-CONICET Buenos Aires, Argentina “Host Specialization of Dengue Virus RNA Structures”
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Mariano Garcia-Blanco Department of Biochemistry and Molecular Biology University of Texas Medical Branch in Galveston TX, USA “The love-hate relationship between host proteins and dengue virus RNAs” Adolfo Garcia-Sastre Icahn School of Medicine at Mount Sinai New York, NY, USA “Influenza virus” Stacy Horner Center for Virology Duke University Medical Center Durham, NC 27710, USA “Intracellular membrane signaling platforms regulate innate immunity to hepatitis C virus” Shahid Jameel Wellcome Trust/DBT India Alliance Hyderabad and New Delhi, India “Hepatitis E virus - A Molecular Understanding” Alessandro Marcello Laboratory of Molecular Virology The International Centre for Genetic Engineering and Biotechnology, Trieste, Italy “Tick-borne encephalitis virus” John McLauchlan MRC-University of Glasgow Centre for Virus Research Glasgow, UK “The Interplay between Virus and Host in Hepatitis C Virus Infection” Glaucia Parahnos Baccala Emerging Pathogens Laboratory Fondation Merieux, Lyon, France “Modulation of target cells induced by Crimean-Congo hemorrhagic fever virus: the contribution in the pathogenesis of the disease”
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ABSTRACTS Abstract title: Sero-detection of Rotavirus and Adenovirus Gastroenteritis Co-infection in children Attending Plateau state Specialist Hospital, Jos, Plateau state Nigeria Authors: Adegboyega Aluko (Department of Virology, UNIVERSITY OF IBADAN)
[email protected] [Presenter], Olabode Atanda (Department of Medical microbiology, Federal college of veterinary and Medical laboratory technology, JOS) Abstract text: This research investigated the prevalence and clinical role of Rotavirus ‘A’ and Adenovirus type40/41 responsible for infantile gastroenteritis in 100 children attending Plateau State Specialist Hospital Jos, with acute diarrhea. Rotavirus ‘A’ and Adenovirus type40/41 were detected as monoinfections 14(14%) samples and co-infections were detected in 18(18%) samples out of 32(32%) positive samples. In terms of frequency, Rotavirus was detected in10 (10%) of the samples followed by Adenovirus type 40/41 4(4%).The samples were analyzed using Vikia Rota-Adeno test kit Biometrieux” Lyon, France. The results obtained were analyzed with SPSS v15.0.There was high prevalence rate of Rotavirus 4(4%) and Adenovirus 40/41 2(2%) in age group 13-24months and the lowest rate of Rotavirus 1% were found in age group 37-48 months. Prevalence rate of Gender and clinical symptoms were significant (p 0.05.
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Abstract title: Envelope protein 2 phosphorylation sites (s75 and s277): a possible candidate for resistance to hcv interferon therapy. Authors: Afzal Samia (National Center of Excellence in Molecular Biology, University of The Punjab Lahore, Pakistan.)
[email protected], Idrees Muhammad (National Center of Excellence in Molecular Biology, University of The Punjab Lahore, Pakistan.)
[email protected] Abstract text: Interferon is considered as the first line of defense against all infections including HCV. The standard treatment for chronic hepatitis C is pegylated interferon alpha (IFN-α) plus ribavirin. Unfortunately 50% of the patients do not respond to the existing treatment but the mechanism underlying this resistance has not been defined. Envelope protein 2 of HCV interacts with the PKR protein. This protein contains a sequence identical with phosphorylation sites of the interferon inducible protein kinase PKR and the translation initiation factor eIF2alpha, a target of PKR. Inhibition of the kinase activity of PKR by this interaction is postulated as a mechanism for the resistance to interferon (IFN) therapy. The present research is focused on the role of envelope protein 2 in causing interferon resistance. The correlation between phosphorylation of envelope protein, PKR protein and interferon response will help to find out the role of envelope genes in mediating interferon resistance.A hybrid in-silico and wet laboratory approach has pointed out serine 75 and 277 of the HCV E2 gene as a promising candidate for the serine phosphorylation. S75 and S277 of E2 protein competes with PKR for phosphorylation. Interferon α-2a response was checked and it was observed that with increase in interferon concentration a significant decrease in number of viable cells was observed in Envelope2 gene mutated at positions S75 and S277 than normal Envelope 2 gene containing cells. This response to interferon was in dose and time dependent manner in cell lines transfected with mutated Envelope 2 genes. The results of this study suggest that serine residues as predicted have a significant role in interferon resistance, especially S75.
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Abstract title: Emergence of genetically variant Hepatitis C virus population in patients with low viral loads (
