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Feb 10, 2018 - International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18). 9th & 10th February 2018 ...
International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

ICMR SPONSORED INTERNATIONAL CONFERENCE ON “MOLECULAR ADVANCES IN BIOMEDICAL RESEARCH (ICMABR- 2018)”

SOUVENIR AND ABSTRACT

EDITORS

Dr. G. Archunan, Organizing Secretary (ICMBAR-18), UGC-BSR Faculty Fellow, Department of Animal Science, Tiruchirappalli.

Dr. K. Saravanan, Organizing Secretary (ICMBAR-18), Assistant Professor of Zoology, Nehru Memorial College (Autonomous), Puthanampatti-621 007. Tamilnadu, India. 9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

INTERNATIONAL CONFERENCE ON “MOLECULAR ADVANCES IN BIOMEDICAL RESEARCH” ICMABR- 2018 PATRON

CHIEF PATRONS Prof. M. PONNAMBALAM, Chairman Er. Pon. BALASUBRAMANIAN, President Shri. Pon. RAVICHANDRAN, Secretary Nehru Memorial College (Autonomous) Puthanampatti.

Dr. A. R. PONPERIASAMY Principal Nehru Memorial College Puthanampatti.

CONVENERS Prof. M. P. SANTHI HOD of Zoology Nehru Memorial College Puthanampatti. Dr. K. SARAVANAN Assistant Professor Dept. of Zoology Nehru Memorial College Puthanampatti.

Dr. K. EMMANUVEL RAJAN Prof & Head Dept. of Animal Science Bharathidasan, University Tiruchirappalli. ORGANIZING SECRETARIES Dr. G. ARCHUNAN UGC-BSR Faculty Fellow Dept. of Animal Science Bharathidasan University Tiruchirappalli.

ORGANIZING COMMITTEE MEMBERS DEPARTMENT OF ZOOLOGY, NEHRU MEMORIAL COLLEGE Dr. P. Neelanarayanan, Associate Professor & Controller of Examinations Dr.V. Ramesh, Assistant Professor Miss. R. Thangamani, Assistant Professor Dr. C. Renuka, Assistant Professor

Dr. N. Ramesh, Assistant Professor Dr. A. Boopathiraja, Assistant Professor Dr. R.Sudha, Assistant Professor Dr. G. Revathi, Assistant Professor Mr. K. Kalaiselvan, Assitant Professor

DEPARTMENT OF ANIMAL SCIENCE, BHARATHIDASAN UNIVERSITY Dr. B. Kadalmani, Assistant Professor

Dr. K. Mahesh, UGC- Assistant Professor

DEPARTMENT OF ENVIRONMENTAL BIOTECHNOLOGY, BDU Dr. S. Achiraman, Assistant Professor DEPARTMENT OF MICROBIOLOGY, BDU Dr. D. Dhanasekaran, Assistant Professor

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

NATIONAL ADVISORY COMMITTEE MEMBERS Dr. S. K. Singh, Prof of Zoology, Dr. T. R. Santhosh Kumar, Banaras Hindu University, Scientist E-II Varanasi, U.P. Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram Dr. Debashish Das, Chief Scientist Dr. P. Geraldine, UGC-BSR Faculty Fellow Stem Cell Research Lab, Dept of Animal Science Narayana Nethralaya, Bangalore Bharathidasan University, Trichy Dr. Lenin Fred, Principal, Dr. R. Thirumurugan, Mar Ephraem College of Engineering & Prof. of Animal Science, Technology, Marthandam. Bharathidasan University, Trichy. Dr. S. Kannan, Prof & HOD of Zoology, Dr. R. Nagarajan, Principal, AVC, Periyar University, Salem Mannampandal Dr. V. Elangovan, Prof & Head Dr. C. Thirunavukkarasu BBAU, Lucknow Asst. Prof of Biochemistry & Molecular Biology, Central University, Pondicherry. Dr. B. Ramalingam, Scientist- D Dr. R. Senthil Kumar, Co-ordinator in National Institute for Research in Tuberculosis, Biochemistry, Royalaseema University, Chennai Kurnool S. Sudhakar, Prof of Biotech. Dr. V. Singaravelu, Associate Professor, Dept. MS University, Triunelveli, of Zoology, Thiruvalluvar University, Vellore Dr. K. Pandimadevi, Associate Professor, Dr. I. Horne Iona Averal, Dean, Dept. of Biotechnology, Holy Cross College, Trichy. Algappa University, Karaikudi. Dr. V. Gokula, HOD of Zoology, Dr. Priscilla Suresh, HOD of Zoology, National College, Trichy. Bishop Heber College, Trichy. Dr. Joseph A. Jerold, Associate. Professor of Dr. T. Nargis Begum, Asst. Prof. of Zoology, Jamal Mohamed College, Trichy. Biotechnolgy, Jamal Mohamed College, Trichy. INTERNATIONAL ADVISORY COMMITTEE MEMBERS Dr. P. Padmanabhan, Deputy Director, Lee Dr. M. Nicoletti, Prof. of Environmental Kong Chian School of Medicine, NTU, Singapore. Biology, Sapienza University, Rome, Itali. Dr. A.M. Shohael, Prof. of Biotec & Genetic Dr. I. Mackraj, Prof. of Medicine & Medical Engineering, Jahangirnagar, University, Sciences, University of Kwazulu- Natal South Africa. Bangladesh. Dr.V. Thiyagarajan, Associate. Professor, Dr. Mohamed Essa, Associate Professor, School of Biological Sciences, The University Food Science and Nutrition, Sultan Qaboos of Hong Kong, Hong Kong. University, Oman. Dr. R. Paulmurugan, Associate Professor, Dr. G. Chandramohan, Associate Professor of Diagnostic Radiology, Stanford University, Community health Sciences King Saud California, USA University, Saudi Arabia Dr. M. Segottuvelan, Research Associate Dr. C. Arunachalam, Associate Professor of Dept. of Animal Sciences, Rutgers University, Medical Bacteriology, King SaudUniversity, New Jersey, USA Saudi Arabia. Dr. Rathnasingh, Senior Scientist, Env. Science Biochemical Team, R& D Center, Daejeon City, South Korea. 9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

9th & 10th February 2018

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

INTERNATIONAL CONFERENCE ON “MOLECULAR ADVANCES IN BIOMEDICAL RESEARCH (ICMABR-18)” 9-10 FEB 2018 PROGRAMME 9TH FEBRUARY 2018 8.30am : Registration 9.30 am : Inauguration Welcome Address : Dr. K. SARAVANAN Asst. Professor of Zoology, Nehru Memorial College Puthanampatti. Introduction about the Conference : Dr. G. ARCHUNAN UGC-BSR Faculty Fellow, Dept. of Animal Science, Bharathidasan University, Trichy. Presidential Address : Dr. A. R. PONPERIASAMY Principal, Nehru Memorial College, Puthanampatti. Inaugural Address : Dr. P. PADMANABHAN Deputy Director, Lee Kong Chian School of Medicine Nanyang Technological University, Singapore. Felicitation : Prof. M.PONNAMBALAM, Chairman, Governing Body Nehru Memorial College, Puthanampatti. Er. Pon. BALASUBRAMANIAN, President, Nehru Memorial College, Puthanampatti. Shri. Pon. RAVICHANDRAN, Secretary, Nehru Memorial College, Puthanampatti. Releasing of Souvenir & : Dr. K. EMMANUVEL RAJAN Conference Abstract Professor and Head, Dept. of Animal Science, Bharathidasan University, Trichy Receiving of Souvenir & : Prof. M.PONNAMBALAM, Chairman, Governing Body Conference Abstract Nehru Memorial College, Puthanampatti. Vote of Thanks : Prof. M. P. SANTHI HOD of Zoology, Nehru Memorial College, Puthanampatti. 11.00 am to 11.15 am : Tea break TECHNICAL SESSION (09.02.2018) 11.15 am to 12.00 pm : Key note Address- I Topic : Translational Molecular Image in biomedical research Resource Person : Dr. P. PADMANABHAN Deputy Director, Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore. 12.00 pm to 1.00 pm : Oral Presentation (OP 1-6) 1.00 pm to 2.00 pm : Lunch Break

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

2.00 pm to 2.45 pm Topic Resource Person

2.45 pm to 3.05 pm Topic Resource Person

3.05 pm to 3.25 pm Topic Resource Person

3.25 pm to 3.45 pm 3.45 pm to 4.25 pm 3.45 pm to 5.00 pm 10.00 am to 10.45 am Topic Resource Person

10.45 am to 11.30 am Title Resource Person

11.30 am to 11.45 am 11.45 pm to 1.00 pm 1.00 pm to 2.00 pm 2.00 pm to 2.30 pm Topic Resource Person 2.30 pm to 3.00 pm Topic Resource Person

3.00 pm to 3.30 pm 9th & 10th February 2018

: Key note Address – II : Stem Cells: Passing the baton from Basics to Clinics : Dr. DEBASHISH DAS, Chief Scientist, Narayana Nethralaya, Bangalore. : Webinar (Onlive Talk) : Biomedical Diagnostic Applications in Translational Research : Dr. R. PAULMURUGAN Associate Professor, Stanford University School of Medicine, California, USA. : Plenary Lecture – I : Angels of Sun (Vitamin-D3): Lights the Vision of Pre-matured Babies : Dr. M. MURUGESWARI Scientist, Narayana Nethralaya, Bangalore. : Tea break : Oral presentation (OP 7-12) : Poster presentation (PP 1-68) TECHNICAL SESSION (10.02.2018) : Key note Address – III : Nanomedicine for Cancer Treatment : Dr. S. KANAGESAN, Institute of Advanced Technology, Universiti Putra Malaysia, Malaysia. : Key note Address – IV : Molecular Mechanism of Lupus Nephratitis : Dr. B. RAMALIGAM Senior Scientist - D, National Institute for Research in Tuberculosis, Chennai. : Tea break : Paper presentation (OP 13-25) : Lunch break : Plenary Lecture – II : Recent Insights into the Basic Biology of Stem Cells and its Therapeutic Advancement : Mr. V. LENIN Bioscience Clinic Middle East, Dubai. : Key note Address – V : Anti-inflammatory effects of non-essential amino acids. : Dr. R. SENTHIL KUMAR, Co-ordinator in Biochemistry, Royalaseema University, Kurnool : Tea break

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

3.30 pm Welcome Address

Conference Report

Valedictory Address

Felicitation

Vote of Thanks

9th & 10th February 2018

: VALEDICTORY FUNCTION : Dr. G. ARCHUNAN UGC-BSR Faculty Fellow Department of Animal Science, BDU. Trichy : Dr. N. RAMESH Assistant Professor Nehru Memorial College, Puthanampatti. : Prof. Dr.P. Shahul Hameed Director and Scientist Center for Environmental Education and Research Tiruchirappalli-620021 : Er. Pon. BALASUBRAMANIAN President, Nehru Memorial College, Puthanampatti. Shri. Pon. RAVICHANDRAN Secretary, Nehru Memorial College, Puthanampatti. Dr. A.R. PONPERIASAMY, Principal, Nehru Memorial College, Puthanampatti. : Dr. K. SARAVANAN Asst. Professor of Zoology, Nehru Memorial College, Puthanampatti.

International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Contents S. No

Title Invited Talk Dr. Parasuraman Padmanabhan Translational Molecular Image in Biomedical Research. Dr. S. Kanagesan Importance of Nanoparticles in Cancer Treatment. Dr. Debashish Das Stem Cells: Passing the Baton from Basics to Clinics. Dr. R. Paulmurugan A molecular imaging biosensor identifies drugs that reactivate mutant-p53 function and enhance combination chemotherapy in cancer. Dr. Senthilkumar Rajagopal Anti-Inflammatory Effects of Non-Essential Amino Acids. Dr. B. Ramalingam Molecular analysis of Lupus Nephritis Kidneys. Plenary Lectures Dr. Lenin Veeraval Recent Insights into the Basic Biology of Stem Cells and its Therapeutic Advancements. Dr. Ponnalagu Murugeswari Angels of Sun (Vitamin-D3): Lights the Vision of Pre-matured Babies.

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P. Arathi and R. Harikumaran Nair Bisphenol- S Induces Reactive Oxygen Species Mediated Cellular Toxicity in Chang Liver Cells. P. Kanimozhi, E. Nevika and Horne Iona Averal Regulation of Hyperandrogenism by Combined Action of Metformin and Pergularia daemia on Lipid and Hormonal Profiles in Albino Rats. M. Angel Mary ., M. Nagalakshmi and M. Rajalakshmi Aspirin-Induced Changes in Fasting Blood Glucose and Biochemical Parameters in Correlation with Histopathological Changes in Pancreas of Albino Wistar Rat- A Dose Dependent Approach. P. Kanimozhi, E. Nevika and Horne Iona Averal Cardioprotective Effect of Stevia rebaudiana on Lipid Profile and Enzyme Levels of Myocardial Infarction Induced Albino Rats. D. Hemasri, S. Sabeena and M. Rajalakshmi Exploring the Antibacterial Activity of Gymnema sylvestreleaves Extract on Escherichia coli and Staphylococcus aureus. R. Krishnamoorthi, E. Natarajan., M. Vigneswaren and S. R. Sivakumar Phytochemical Screening and in Vitro Antibacterial Activity of Naravelia zeylanica L. against Plant Pathogens. R. Mohamed Asik, N. Suganthy., R. Thirumurugan., P. Padmanaban and G. Archunan Anticancer Potential of RgO-ZnO Nanocomposite Synthesized by Hydrothermal Method using Biogenic Source against Cervical Cancer. R. Cecily Rosemary Latha and V. A. Jemi Feiona A Study on Isolation and Culturing of Mesenchymal Stem Cells from the Omental Tissue. S. Alagendran., N. Pushpa and G. Archunan Salivary Metabolomics Screening of Putative Biomarkers during early Diagnosis of Alzheimer's Disease. Srinivasan Mahalingam., Salha Maheen., Kalaivani., Saranya Uruthirapathy., Nargis Begum Tajuddin., Padmanabhan, P and Govindaraju Archunan Analysis Of Salivary Organic Acid In Buffalo With Reference To Estrus. Saranya Uruthirapathy., Salha Maheen., Nargis Begum Tajuddin., Shilu Mathew., N. Thajuddin and Muhammad Ilyas Extracellular Polymeric Substances from Microalgae and Their Biotechnological Potential. Rini Raphael and Umamaheswari Sepperumal

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Surfactant Induced Skeletal Anomalies in Puntius parrah Larvae. S. Umamaheswari and M. Puspavalli Utilisation of Polyethylene Terephthalate for Production of Cellulose by Bacillus Licheniformis. J. Arockia John Paul., B. Karunai Selvi., M. Biruntha and J. Archana RAPD Profiling of Indigenous Earthworm Resources of Sivagangai District. M. V. Gayathri., P. Josephin Jancy Rani and A. Avila Jerley Biosynthesis and Characterization of Red Pigment from Monascus purpureus and its Antioxidant and Antitumor Property. P. Uma Mageshwari and A. J. Bhorgin Lourdu Mary Extraction and Evaluation of Antimicrobial and Hemolytic Activity of the Coelomic Fluid of Lampito mauritii and Examination of its Biomolecules using GC-MS Analysis. C. Senthamil Selvan Assessment of Radiation Dose by Biodosimetry. S. Nithyananthan and C. Thirunavukkarasu Anticancer Potential of Glibenclamide: It’s Synergistic Cytotoxicity with Doxorubicin on Liver Cancer Cells. S. Josephinol ., T. Nargis Begum., M.H. Muhammad Ilyas., Shilu Mathew., Aakila Shahul and Neeraja Anandamyle Antidiabetic Effect of Ethanolic Leaves Extract of Azima tetracantha Lam. in Streptozotocin Induced Diabetic Rats. S. Kalaivani., R. Muthu Selvam., and S. Chandru Isolation and Screening of Plant Growth Promoting Rhizobacteria from Tomato Rhizosphere Soil. G. Kasanraj., A. Prithivrajan., R. Muthu Selvam and G. Archunan Influence of Parity Status on Urinary Protein Profile and its Dynamics in Murrah Buffalo. V. Silambarasan., G. Deepalakshmi and G. Archunan Observation of Courtship Behaviors in White Yorkshire Pig in Reference to the Substantiation of Estrus Phase. Anisha Marina., Rajini, M., M. Kumar., Jeyapriyadharshini, A., Ludas., S. Indu., A. Anita Roy and M. Rajalakshmi Novel Triterpenoids from Cassia fistula l. Regulate P53 and ERK2 Genes to Induce Apoptosis in HT-29 Colon Cancer Cells. S. Thilagameena., G. Smilin Bell Aseervatham andT. Sivasudha In Vitro and In Silico Antioxidant and Hepatoprotective Potential of Pouteria campechiana. G. Chandru and S. Sethupathy Anti- steriodogenic and withdrawal effect of Thespesia populnea (L.) on female genital tract in mice. Poster Presentation (Pharmaceutical Biology & Biotechnology) Aakila Shahul., Neeraja Anandamyle., Girija Nagarajan., Nargis Begum Tajuddin., Muhammad Ilyas and Mohamed Husain. The Influential Role of Probiotics in Rejuvenating the Affected Healthy Cells by Neoplastic Chemotherapy Treatment. G. Priya and K. Saravanan Post-Implantation Changes in the Uterus of Rats with Response to Thespesia populnea Bark Extracts treatment. G. Revathi., S. Elavarasi., K. Saravanan and M. Manisha Phytochemical Screening, GC-MS Analysis and Toxicity study of Antidiabetic Polyherbal Drug. Rajathi D Modilal and Devi, K Evaluation of Anticariogenic Effect of Premna latifolia Leaves. C. Renuka and K. Saravanan, K Antidiabetic Potential of Biophytum sensitivum whole plant extracts in STZ Induced Diabetic Rats. P. Persiyal and Priscilla Suresh Effect of Couroupita guianensis Extract on Growth of Urinary Calculi Struvite Crystallization. P. Karuppannan, K. Saravanan and M.P. Santhi Docking of Dibutyl Phthalate Isolated from Ventilago maderaspatana for the Treatment of Diabetes Mellitus. Nigamananda Dey., Nayef Louri ., K. Sathiya Priya and Kadalmani Balamuthu Use of Allogeneic Cultured Keratinocytes as an Adjunctive Therapeutic Option in Management of

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Partial Thickness Deep Burn in Children - Initial Clinical Experience at BDF Burn Unit. M. Rajeswari ., K. Sathiya Priya ., T. Ramani Devi and B. Kadalmani Molecular Analysis of DES Induced Uterine Fragments by Injection Model in Mice Treated with MMP 2 & 9 Inhibitors-I. R. Arul Priya ., K. Saravanan ., M.A. Akbarsha., B. Umarani and Egbuna Chukwuebuka In vitro Anticancer Activity of Pisum sativumEthanolicSeed Extract against MCF-7 Cancer Cell Line. R. Cecily Rosemary Latha and Renganayaki In Vitro, In Vivo and In Silico Analysis of Anti-inflammatory and Antimicrobial efficacy of Curculigo orchioides Rhizome. V. Balan ., K. Saravanan ., B. Umarani and M. Dhivya Sri Collection and Data-Mining of Bioactive Compounds with Cancer Treatment Properties in the Plants of Rutaceae Family. M. Divya ., A.V. Muralitharan ., S. Pushpa and A. Babby 3D Vasculography – A Precise Diagnostic method of Cardio Vascular Diseases. Biochemistry, Immunology and Chemical Signaling N. Elavarasan ., K. Kokila and V. Sujatha GC-MS analysis and identification of bioactive compounds from Sechium edule root extracts. G. Revathi ., S. Elavarasi ., K. Saravanan and R. Shanmugalakshmi Phytochemical Screening and GC-MS Analysis of Ethanol Extract of Polyherbal Drug. N. Pushpa and S. Alagendran Oxidative stress biomarkers in serum of patients with PCOS. B. Sumathi and V. Ramesh Scanning Electron Microscopic Studies on the Silk Gland of Dicloxacillin Treated Diseased Silkworm Bomybyx mori (L.) (Bombycidae). M. Muthu Meenal ., M. Biruntha ., J. Archana and J. Arockia John Paul Changes in the Protein Profiling of Earthworm, Eudrilus eugeniae under Toxic Substrates Stress. V. Vinotha ., M. Biruntha ., T. Sangeetha., J. Archana and J. Arockia John Paul Immunomodulatory Effect of Coelomic Fluid of Two Earthworms after Exposure to Nanoparticles and Microbial Stress. M. Srinivasan., C. Manikkaraja., P. Kalaivani., S. Muniyasamy and G. Archunan Confirmation of acetic acid in urine as estrus indicator of buffalo adopting HPLC methods. V. Silambarasan and G. Archunan Identification of Estrus-specific Pheromone Compound in Pig, Susscrofa domesticus, by GC-MS Analysis. V. Silambarasan., A.P. Swetha., N. Bavani and G. Archunan Development of Pheromone Based Live Trap for Rats – A Key for Pest Management. R. Jayaraja., G.Karthikeyan., C. Manikkaraja., G. Saibaba., G. Pugalenthi and G. Archunan Cattle Pheromones and their Applications. Microbiology, Nanotechnology & Stem cell Biology M. Gowsalya., G. Vinothini and D. Dhanasekaran Bacteriocinogenic Potential of Actinobacteria against Poultry Pathogens. P. Maheswari., M. Jaquline Mary and M. Rajalakshmi In-Vitro Study of Antimicrobial Activity of A Novel Triterpenoid Derivative Isolated from Ethyl Acetate Extract of Cassia fistula Stem Bark with Respect to Morphological and Molecular Analysis. C. Ireen., R. Arokiya Anitha Mary., V. Mariya Reyoniya., S. Rahamah Nisha., P. Thianisa Mary and M. Rajalakshmi Elephantopus scaber - A Potential Antibacterial agent by In-Vitro and In-Silico Approaches. G. Muthulakshmi and P. Neelanarayanan Phytochemical Screening and Antibacterial Activity of Methanol Leaves Extracts of Coccinia grandis against Fish Pathogens. Nargis Begum Tajuddin., Muhammad Ilyas., Salha Maheen., Saranya Uruthirapathy and Shilu Mathew. Potential Pharmaceuticals and Biomedical Applications of Diatoms Microalgae. Gabriella Maria Dumenil and Sujatha Ilangovan

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Antimicrobial Activity of Mesenchymal Stem Cells Isolated from Wharton’s Jelly (Umbilical Cord) Against Pseudomonas aeruginosa, Staphylococcus aureus and Escherichia coli in vitro. D. Jeeva., S.Jasmine Suba and T. Shalini Gnanam A Comparative Study of the Antimicrobial Activity of Azadirachta indica Leaf Extract & Bacillus cereus (Probiotics) on Selected Prawn Pathogens. G. Priya and P. Deepa Antifungal Potentials of Medicinal Plants: A Review. B. Karunai Selvi., J. Arockia John Paul and M. Biruntha Cellulolytic Efficiency of Fungal Species Isolated From Vermicompost. K. Amirthalakshmi., M. Srinivasan., G. Vinothini., D. Dhanasekaran and G. Archunan Screening of Vaginal Bacterial Profile from Buffalo during the Eestrous Cycle. A. Venkatesan., G. Rupashree., P. Priyatharshini and V. Sujatha Synthesis of Copper Oxide Nanoparticles from Lagerstroemia speciosa Leaves Extract and its Antibacterial Activity. V. Sujatha., A. Venkatesan and S. Kowsalya Greener way Synthesis of Copper Oxide Nanoparticles from Tribulus terrestris Flower Aqueous Extract and its Antibacterial Activity. Sulaiman Ali Alharbi., M.E. Zayed., Arunachalam Chinnathambi., S. Naiyf ., Alharbi and Milton Wainwright Evaluation of the Microbiological and Physicochemical Quality of Artesian Well Water Used for Irrigation in ArRiyadh. S. Prakash., M. Sowndharya and V. Sujatha Phytochemical Profile, Antimicrobial and Larvicidal Activity of Andrographis echioides (L) Leaf Extracts. B. Sowmiya., A. Priya Aarthy and S. Banusrie Molecular docking study on flavonoids and their derivatives as inhibitors of PagN, a virulence factor of Salmonella typhimurium. J. Belinda., P. Carolin Rubina Jency and M. Rajalakshmi Biosynthesis, Characterization and Antibacterial Efficacy of Silver Nanoparticles from Phyllanthus niruri. Neeraja Anandamyle., Aakila Shahul., Girija Nagarajan., Nargis Begum Tajuddin., Shilu Mathew and Muhammad Ilyas Nanoparticle-Assisted – Stem Cell Therapy for Neurological Disorders. R. Hemalatha., S. Indu and M. Rajalakshmi A Comparative Analysis of Antimicrobial Activity of Biosynthesized Silver Nanoparticles and Gymnemic Fraction Isolated from the Acetone Leaves Extract of Gymnema sylvestre. P. Punitha and A. Boopathy Raja Phytochemical Screening and Antioxidant Activity of Zn-Cu Nanocomposite Synthesized from Flower Extract of Ixora coccinea. A. Boopathy Raja Phytochemical Screening and Cytotoxic Effect of Bougainvillea glabra Flower Fraction and Mg Doped ZnS Nanoparticle on Rin 5f and 3t3 L1 Cell Lines. A. Boopathy Raja., B. Keerrthiga and D. Aishwarya Evaluation of Antioxidant and Antibacterial Activity of Cu Doped MgO Nanoparticle. M.P. Santhi., A. Boopathy Raja., S. Radhika., D. Swarna bharathi and V. Ramesh Evaluation of Antibacterial Activity of Ti Nanoparticle against Different Bacterial Strains. D. Rajathi Modilal and S. Mahalakshmi Therapeutic Property of Silver Nanoparticles Synthesized using Premna latifolia Leaves. Lekshmi Krishna., Murali Subramani., Murugeswari Ponnulagu., Nallathambi Jeyabalan., Rohit Shetty., Samanta Sekhar Khora., Debashish Das Decellularized Human Amniotic Membrane Exhibits Protective Role under Oxidative Stress to Maintain the Cell Functionality of Cultured Arpe-19. P. Ida Princy., T. Maria Sylvia and S. Kamalakkannan Evaluation of Microbial Populations and Protein Profile across the Estrous Cycle in Mus musculus. R. Priyadharshini., S. Lashmi Prabha., S. Tamilsurya., G. Manikkaraja., M. Sivakumar and G. Archunan

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In vitro antibacterial and anticancer potential of L-histidine functionalized silver nanoparticles. K. Jeremiah Kirubananth., I. Joseph Antony Jerald., R. Ponmalar and R. Lavanya Comparative evaluation of volatile compounds from the pheromone source (urine) between Prepubertal and Intact Male: Capra hircus. Environmental Biology & Wildlife disease G. Manjula and K. Saravanan Effect of Environmental Volatility on Health Status of Poultry Birds with Special Reference to Egg Production. T. D. Iswarya and Horne Iona Averal Targeting Industrial Wastewater Reuse With Reference to Water Analysis and Histopathological Study in Oreochromis mossambicus. K. Govindaraj and I. Joseph A. Jerald Seasonal Study on the Physical, Chemical and Biological Characteristics of River Cauvery, Tiruchirappalli District, Tamilnadu, India. C. Kanimozhi and V. Ramesh Diversity of Butterfly and Their Host Plants in Namakkal District. K. Pushparaj and V. Ramesh Growth and Feed Efficacy of Silkworm Bombyx mori (L.) Larvae Fed the MR2 Mulberry Leaves Fortification with Different Nutritional Supplementary Compounds. N. Ramesh Survey of Helminth Infections in Sheep (Ovis aries. L) at Puthanampatti Area of Trichy District, Tamilnadu, South India. P. Premalatha., K. Saravanan., P. Karuppannan and M.P. Santhi Phylogenetic Analysis of Freshwater Mussel (Bivalvia: Unionoida). Anumol Anto., S.R. Vasugi and S. Ganga Generation of Intrinsically Coloured Silk by Silkworm Bombyx mori Using Dye Mixed Diet. R. Krishnamoorthy and O. Basith Assessment of Well Water Quality in Chatankuppam Village, Tiruvallur District. G. Lakshmanan., K. Saravanan and B. Umarani A Preliminary Survey of Grasshopper in Grasslands of Kottathur, Trichy District. O. Basith., S. Christobher., M. Periyasamy., H.E. Syedmohamed., A. Sadiq Bukhari and R. Krishnamoorthy Activity Concentration of 210po and, 210pb in Different Aquatic System, Tamil Nadu. Mohd Younis., A. Vijaya Anand and K. Sasikala Colonoscopy Screening Guidelines on the Diagnosis of Colorectal Cancer. K. Kalaiselvan and N. Ramesh Social organization of Indian Peafowl (Pavo cristatus) in Thuraiyur area of Trichy District, Tamilnadu. Vector Biology T. Chinnamani and A. Jeyasankar Antifeedant and Larvicidal Activities of Ethyl Acetate Extract of Jatropha integrrima Against Spodoptera litura and Helicoverpa armigera. A. Chennakesavan., C. Kanimozhi.,V. Ramesh and P. Rajasekar Larvicidal Activity of Plant Extract against Anophelea stephensi and Aedes aegypti. P. Veerasamy and A. Jebanesan Eco Friendly Impact of Phytochemicals Derived from Marine Biodiversity for the Control of Filarial Vector Population. V. Shoba Chemical Composition and Anti-Mosquito Potential of Catharanthus roseus Leaves Extract against Larvae of Aedes aegypti. P. Shahul Hameed

Molecular Mechanisms Controlling the Circadian Rhythm.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Invited Lectures

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Translational Molecular Mol Image in Biomedical omedical Research Dr. Parasuraman Padmanabhan Deputy Director, Lee Kong Chian School of Medicine, Nanyang Technological University (NTU), Singapore -636921 Early detection, disease progression and therapeutic efficacy need to be monitored invivo using some, cellular or molecular activity that can serve as an underlying biomarker, and this requirement has posed a major challenge to traditional imaging technologies that have been developed over the last decade, which mainly encompassed anatomical and phy physiological readouts. Non invasive multimodal molecular imaging has emerged as one of the dynamic aiding tool in medicine both in diagnosis and therapeutics. Each imaging modality has its strengths and application in preclinical/clinical imaging of cellular and molecular activities. This talk will mainly focus on the current development and relevance of novel molecular approaches for in vivo imaging at diverse levels, from the organ to the cellular level in preclinical translational or Clinical research.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Importance of Nanoparticles in Cancer Treatment Dr. S. Kanagesan Department of Physics, Faculty of o Science, Universiti Putra Malaysia, 43400 UPM Serdang, Malaysia. Email: [email protected]

Recent improvements in the field of nanotechnology have led to the development of advanced materials for effective detection of both diagnostic and therapeutic properties to the cancer treatment. In future, the he use of nanotechnology in cancer therapy will potentially reduce the side effects, mortality rates, rates and increase the patient survival. The nanoparticles will play a critical role in the drug delivery, in vivo clinical diagnosis and monitoring of pharmacological and cell-baseddiagnosis. This his technology, however, was developed from far wider in-vitro in and /or in-situ applications.. More often than not, tthe latest development of a multifunctional unit of nanometer dimensions is capable of undertaking a comprehensive range of tasks such as a fastdrug-delivery to destruct cancer cells. Multifunctional nanoparticles have ha the ability to target the delivery of drugsto to the tumor by surface functionalization. In particular rticular, they treat cancer modalities, therefore, perform perfo therapy and diagnosis simultaneously. simultaneousl Multifunctional nanoparticles basically target to tumors and have the potential to bypass the healthy cells. Keywords:

Nanotechnology; anotechnology;

multifunctional

nanoparticles;

targetingtherapy;

Nanoparticle synthesis; drug delivery;

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Stem Cells: Passing the Baton from Basics to Clinics Dr. Debashish Das, Ph.D Chief ief Scientist, Narayana Nethralaya, Nethralaya Stem Cell Research Laboratory Bangalore- 560 099 – Karnataka, INDIA E-Mail: [email protected]; [email protected] Limbus is the region in the cornea cornea-scleral scleral border harboring the ocular stem cells. These ocular stem cells along with cells from conjunctiva and oral mucosal origin are widely used as a therapeutic modality for corneal surface damage. Though the process of therapy has been standardized and well established the outcome of the treatment remained remained variable, for several reasons including variability in culture methodology. Not much is known about the processes defining the success of the outcome. Panels of markers are used to identify the differentiated as well as progenitor cells used for transplantation transplantation on human amniotic membrane. We have developed a protocol that could ould unequivocally promote differentiation of cells from a limbal, conjunctival or oral mucosal biopsy into the corneal lineage. These findings would provide knowledge for better as well as successful stem cell therapy module at least more so in the cases where epithelial origin cells are being proposed for cell therapy. The study outcome on a futuristic note would also envisage for personalized therapy avenue. This study would also als aid in improvising the treatment procedure of limbal transplantation thereby probably increasing the rate of success.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

A molecular imaging biosensor identifies drugs that reactivate mutant-p53 function and enhance combination chemotherapy in cancer Dr. R. Paulmurugan Associate Professor, Department of Radiology, Stanford University, School of Medicine, California, USA. Phone: 650-725-6097, Fax: 650-721-6921, E-mail: [email protected]

Introduction: Misfolding mutations in the tumor suppressor p53 are found in >50% of human cancers, including glioblastoma (GBM). Small molecule drugs that bind to mutant p53 (mt-p53) and induce refolding to a wild-type p53 (wt-p53) conformation may restore its biological function. We developed a molecular imaging biosensor that detects drug-induced structural changes in p53 protein in cells and in vivo. We validate the specificity of this biosensor for drugs that efficiently bind to various mt-p53 proteins and recover their function. We also evaluate the potential role of these drugs in sensitizing GBM cells that are endogenously mutant for p53 protein but that we subsequently engineered by stably transducing with four different biosensors to have the corresponding genetic backgrounds (wt-p53 or three other misfolding-mtp53s). Methods: We developed separate Ln229 cells (endogenously mt-p53P98L) each to stably express one of four varieties of a split-Renilla luciferase reporter protein complementation biosensor (NRLuc-p53-CRLuc), to thus achieve four different cellular p53 statuses (wt-p53, mtp53Y220C, mt-p53G245S and mt-p53G282W). We first studied if these transduced fusion proteins could induce changes in the background p53 cellular status. We then evaluated these cells for luciferase complementation after exposure to drugs that induce structural changes in mt-p53 protein (PhiKan083, a drug of high affinity for p53Y220C, and SCH-529074, a small molecule of high affinity for p53G245S). We used RITA, a small molecule that stabilizes p53 protein without direct binding as a control to prove the specificity of our sensor. Further, we tested the apoptotic effects of these drugs when combined with standard chemotherapies in all transduced Ln229 cells, as well as wild type U87`MG cells (that are endogenously wild-type for p53 status). Results: Ln229 cells changed their endogenous phenotype with respect to p53 status by adoptingap53 pheno type that reflects the transduced biosensor fusion protein. There was activation of p53 structural change and associated luciferase signal when we treated cells with PhiKan083 and SCH-529074. Indeed, cells treated with PhiKan083 showed significant levels of p53 refolding not only formt-p53Y220C, as expected, but also in cells transduced with thewtp53and all other mutant biosensors. The Ln229 cells treated with doxorubicin (Dox) 9th & 10th February 2018

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

ortemozolomide (TMZ) in combination with PhiKan083 (12.5 (12.5-50µM) M) or SCH (1.25-5.0µM) (1.25 showed significant dose dependent enhancement of apoptotic effect (>40%increase) within 24 h after treatment when compared to respective drugs as monotherapy, an effect also observed in U87MG glioblastoma cells. Conclusion: A molecular imaging biosensor consisting ofp53 of protein flanked bysplit luciferase fragments and expressed as a fusion protein maintains its particular p53 function(whether wild-type type or mutant) within cells, andalso induces achange a inthe genetic background of cells with respect to that p53 status accordingly. The use of PhiKan083 and SCHSCH 529074 improves the therapeutic effects of Dox and TMZ in Ln229 and U87MG cells.Applications of this biosensor will be usefulfor discovery of new p53anti-misfolding p53 drugs and subsequent validation of drug hits in small animal models.

Figure 1: a) Schematic figure illustrating the functionality by which Split-Renilla Split luciferase complementation sensor differentiate folded and misfolded proteins in cells. b) Fluorescencee microscopic images of Ln229 cells stably expressing Split-Renilla Split luciferase complementation biosensor at equal level of biosensor fusion protein. c) The level of complementation signal in Ln229 cells stably expressing wild type and mutantmutant p53 biosensors. d) Optical bioluminescence imaging of Ln229 cells stably expressing wt-p53 p53 biosensor after treated with small molecule drugs PhiKan083 (125 µM) and SCH-529074 (5 µM). M). e) Quantitative graph showing the bioluminescence signal measured from image in ‘d’.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Anti--Inflammatory Effects of Non-Essential Essential Amino Acids Dr. Senthilkumar Rajagopal, PhD, MRSC., [London] Co-ordinator Department of Biochemistry, Rayalaseema seema University, Kurnool, AP Email: [email protected]

Glycine is an effective anti-inflammatory, anti inflammatory, immunomodulatory, and cytoprotective agent. Glycine blocks the increases in intracellular calcium ions in specific cells caused by many structurally different stimuli like alcohol, endotoxin, peptidoglycan, polysaccharide, bile acids, peroxisome proliferators, etc. etc The mechanisms behind by which glycine protects remain incompletely understood. Aim: Aim The aim of the present study was to investigate the antiinflammatory effect of glycine, a non non-essential amino acid in an in vitro model and the PKC isozymes responsible for the action of alcohol were investigated using translocation as a measure of activation. Methodology:: Human Umbilical Vein Endothelial cells damage was induced by an ethanol (5%) and glycine lycine was subsequently administered at a dose of 300 µM for 24 hrs. Translocation of PKC isozymes to the membrane studied by western blot. Results: Administration of alcohol significantly elevated the inflammatory cytokines Tumor necrosis factor-α (TNF-α)) and Interleukin 6 (IL (IL-6), 6), activity of myeloperoxidase and depleted reduced glutathione (GSH) concentration compared with control group.. Subsequently, glycine supplementation to alcohol induction significantly lowered the TNF-α α and IL-6, reduced the activity of myeloperoxidase and normalized the glutathione concentration compared with untreated alcohol induction groups. groups Exposure of the cells to ethanol (5%) led to the translocation of PKC ε. Conclusion: Thus, the present study demonstrates that inhibitory effect of glycine by virtue of its ability to optimize the activity activit of myeloperoxidase, normalize the concentrations of inflammatory cytokines and reduced glutathione. glutathione To understand the physiological significance sig of PKC ε translocation to the membrane by alcohol, further studies are warranted to elucidate the mechanisms involved in the activation of calcium channels. Keywords: Alcohol, amino acids, antioxidants, calcium channels, glycine, protein kinase C

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Molecular analysis of Lupus Nephritis Kidneys. Dr.Ramalingam, Dr. B. Scientist-D National Institute for Research in Tuberculosis, Chennai. Email:[email protected]

Lupus nephritis is a devastating complication of Systemic Lupus Erythematosus for which current therapies are insufficiently effective. Integrated immunologic, genomic and proteomic approaches may yield new insights into disease pathogenesis and thereby improve therapeutic strategies for lupus nephritis. Given the lack of sequential biopsies from humans, it also remains essential to study informative animal models of disease. Cross species analyses can identify cells or pathways that are relevant to human disease and can be further studied in mouse models. Using a systems biology approach approach in which we compare molecular data from kidneys of 3 different mouse models of lupus nephritis with data from human lupus biopsies, we have found that inflammatory events escalate rapidly around the time of proteinuria onset. This is followed by hypoxia xia and metabolic stress, and by tubular and endothelial dysfunction. The failure of complete reversal of these abnormalities may increase the sensitivity of the kidney to further insult. We further found that renal macrophages and dendritic cells (DCs) are ar key players in lupus nephritis both in mouse models and humans and that macrophages display a hybrid molecular profile that reflects incomplete resolution of inflammation and excessive tissue remodeling. Finally, our studies have suggested several new biomarkers biomarkers for disease stage that can now be tested longitudinally in human SLE patients

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Plenary Lectures

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Recent Insights into the Basic Biology of Stem Cells and its Therapeutic Advancements. Mr. Lenin Veeraval Molecular Biologist, Bioscience Clinic Middle East, Dubai, United Arab Emirates. Email: [email protected]

Understanding of Stem cells for their biological properties and its potential therapeutic importance is always fascinating and ever expanding in diverse biological fields like Neuroscience, Cancer, Ageing etc. Maintenance of the tight regulation of self renewal and differentiation by the parent stem cells, stimulus derived stem cell activation and quiescence, distinguishing phenotypes of normal and cancer stem cells are some of the basic questions that has been raised a decade ago and new exciting answers are still coming up to prove and disprove the existing concepts. In the aspect of therapeutics, Mesenchymal stem cells (multipotent stem cells) studied well for cell based therapies and regenerative medicine for the treatment of skeletal tissue repair like scar, hair loss and face wrinkles. Recent reports suggest MSC possess immune modulatory functions which help in effective vaccination. Having these promising therapeutic outcomes of MSC, it is the time for stem cell research community to invest even more interest to study on how degenerative disorders and cancers can possibly be treated with stem cell based approaches.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Angels of Sun (Vitamin-D3): Lights the Vision of Pre Pre-matured Babies. Dr. Ponnalagu Murugeswari. Murugeswari Scientist, Stem Cell Laboratory, GROW Lab, Narayana Nethralaya, Bangalore-99. Email: [email protected]

Vitamin D is a fat-soluble soluble vitamin that benefits our our bones, our immune system, and eyes too. Vitamin D3 is mainly synthesized from the conversion of the skin precursor 77 dehydrocholesterol (7DHC) to vitamin D3 by ultraviolet (UV) B sunlight. Extrarenal synthesis of vitamin D3 has been reported in many tissues tiss and cells. Vitamin D deficiency is common in many vascular eye diseases such as age related macular degeneration, diabetic retinopathy and retinopathy of prematurity. Vitamin-D D deficiency has been associated with the pathological angiogenesis. Oxidative ve stress plays a crucial role in pathologic derailed retinal vasculature. In retinopathy of prematurity, hyperoxia hyperoxia leads to regressed vessels and hypoxia induces neovascularization. Vitamin-D D3 supplementation is known to modulate angiogenic factors in these se oxidative stress conditions. The preterm babies (10-20%) (10 20%) do develop retinopathy of prematurity and majority of them are vitamin-D vitamin 3 deficient. The supplementation of vitamin vitamin-D3 in early stage of retinopathy of prematurity may act as a preventive measure in these preterm babies.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

ABSTRACTS

Oral Presentation

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

[OP-1] Bisphenol – S Induces Reactive Oxygen Species Mediated Cellular Toxicity in Chang Liver Cells. Arathi, P and Harikumaran Nair, R* Physiology Research Laboratory, School of Biosciences, Mahatma Gandhi University, Kottayam, Kerala, India - 686 560 Email: [email protected]. Mobile No: 9447260362. Bisphenol S (BPS), an organic compound widely used for the production of polycarbonate plastics and epoxy resins. BPS is an emerging environmental contaminant and a well known endocrine disrupting compound. The exposure of BPS to hepatocytes may impair the xenobiotic mechanism. During the detoxifying process, different types of free radicals are produced. We analyzed the exposure of BPS in different concentration on hepatocytes in order to understand the production of reactive oxygen species (ROS) and ROS mediated cellular modifications. Seven groups of chang liver cells were selected and grouped as normal, vehicle control, BPS treated with 100, 200, 300, 400 and 500 µM respectively. Hepatocytes were incubated with BPS for 48 hrs and different parameters were analyzed. Enhanced ROS production was observed by DCF - DA staining. The enzyme activity of free radical scavenging enzymes, superoxide dismutase and catalase were decreased. It was observed that glutathione and glutathione peroxidase levels were decreased during the exposure dose of BPS was increasing. The over production of ROS leads to depletion of antioxidant activity of the cells which in turn results in membrane damage as evidenced by increased production of MDA and leakage of LDH from the cells. From the study, it is confirmed that the dose dependant exposure of BPS results in reduced cellular viability and apoptosis. Keywords: Bisphenol S, change liver cells, reactive oxygen species, apoptosis.

[OP-2] Regulation of Hyperandrogenism by Combined Action of Metformin and Pergularia daemia on Lipid and Hormonal Profiles in Albino Rats. Kanimozhi, P, Nevika, E and Horne Iona Averal PG and Research Department of Zoology Holy Cross College (Autonomous), Tiruchirappalli.

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Polycystic ovary syndrome is one of the most common female endocrine disorders leading to infertility. The insulin sensitizing treatments such as metformin leads to the improvement of the fertility. They have several sites of action like steroid secretion by ovarian cells, insulin sensitivity in muscle, adipocyte and fat redistribution. According to the present study the metformin has a good healing effect on PCOs condition. Thus it is a potential merit as the first line of treatment for ovulation induction. But to increase the recovering time and minimizing the side effects phytotherapeutic approach can be added to the treatment. Herbal drugs have promising role in the treatment of PCOs and has minimal side effects. They also enhance the immunity of the body and also regularize menstrual cycle. So metformin is used as a co-treatment with the plant Pergularia daemia which improves the glucose level, lipid profile such as LDL, HDL, Cholesterol and triglycerides, and also it recovers the action of hormones such as FSH, LH, estradiol, progesterone and testosterone. From the results it is evident that combined therapy of metformin and Pergularia daemia is better than metformin treatment alone. Keywords: Polycystic ovary syndrome, infertility, metformin, Pergularia daemia, lipid and hormonal profiles.

[OP-3] Aspirin-Induced Changes in Fasting Blood Glucose and Biochemical Parameters in Correlation with Histopathological Changes in Pancreas of Albino Wistar Rat- A Dose Dependent Approach Angel Mary, M1, Nagalakshmi, M1 and Rajalakshmi, M1, 2* 1

2

PG and Research Department of Zoology, Holy Cross College (Autonomous), PG and Research Department of Biotechnology & Bioinformatics, Holy Cross College (Autonomous), Tiruchirappalli, Tamilnadu, India. Email: [email protected]

Aspirin (acetylsalicylic acid, ASA) has been used as one of the most famous, cheap, easily available and widely used Non Steroidal Anti Inflammatory Drug (NSAID). Aspirin and the other NSAIDs do not generally change the course of the disease process in those conditions where they are used for symptomatic relief. The pain killer effect of aspirin is best known for its effects on the two cyclooxygenase enzymes (COX1 & COX2), but, recently, aspirin could specifically inhibit the protein I-kappa-B-kinase beta (IKK-beta). This kinase is used for its role in the cascade of signals that activate the nuclear factor kappa-b (NF-kappa-B) family of cellular genes which regulate inflammatory and immune responses. Now, it turns out that IKK-beta also works in another pathway to contribute to insulin resistance by interfering with insulin signaling. 9th & 10th February 2018

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Hence the aim of the present study is to observe effects of the anti- inflammatory drug aspirin of various doses on pancreas of young albino Wistar rats, by observing histopathological studies in pancreas and by estimating the serum glucose, insulin and C-peptide levels. Administration of aspirin progressively increases the blood sugar level and alters other biochemical parameters. The cells of the Islets of Langerhans of the normal rats had round vesicular nuclei and pale pink cytoplasm. But the cells of the diabetic animals had pycnotic nuclei. Though aspirin is used as therapeutic purpose for pain relief even for diabetic patients, our results suggest that repeated long term administration of higher concentration could cause the insulin resistance followed by the alterations in the biochemical parameters and that high concentration may cause pancreatic damage. Hence, a mild dose alone is always safe Keywords: Aspirin, Blood sugar, pancreas, C-peptide, insulin, β-cells.

[OP-4] Cardioprotective Effect of Stevia rebaudiana on Lipid Profile and Enzyme Levels of Myocardial Infarction Induced Albino Rats Kanimozhi, P, Nevika, E and Horne Iona Averal PG and Research Department of Zoology Holy Cross College (Autonomous) Tiruchirappalli. Plants are being used for the treatment of myocardial infarction since antiquity. Myocardial infarction is the leading cause of death. In the present study, cardioprotective effect of aqueous extract of Stevia rebaudiana leaves in isoproterenol induced myocardial infarction in albino rats was evaluated. In vivo effect of aqueous extract of S. rebaudiana was evaluated in albino rats by isoproterenol induced myocardial injury model. Serum cholesterol, HDL, LDL, triglycerides, SGOT, SGPT, LDH, catalase, glutathione peroxidase, gamma glutamyl transpeptidase, acid phosphatase and alkaline phosphatase were evaluated in each group and compared using appropriate statistical tests. Isoproterenol produced significant myocardial infarction as compared to normal control group. Administration of S. rebaudiana recovers the biochemical profiles as compared to disease control group. The aqueous extract of S. rebaudiana was found to be cardioprotective against myocardial injury. Keywords: Cardioprotective effect, Stevia rebaudiana, Isoproterenol, Myocardial infarction, biochemical profiles. 9th & 10th February 2018

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

[OP-5] Exploring the Antibacterial Activity of Gymnema sylvestreleaves Extract on Escherichia coli and Staphylococcus aureus Hemasri, D1, Sabeena, S2 and Rajalakshmi, M1, 2* 1

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PG and Research Department of Zoology, Holy Cross College (Autonomous), PG and Research Department of Biotechnology & Bioinformatics, Holy Cross College (Autonomous), Tiruchirappalli, Tamilnadu, India. Email: [email protected]

Medicinal plants as a whole or their bioactive compounds are referred in the ayurvedic literature for their tremendous clinical application on infectious diseases. The chemotherapy involving the usage of chemical drugs with drastic side effects could be denied from choosing an alternative approach with phytotherapeutics. Gymnema sylvestre (G.sylvestre) commonly named as periploca of woods is one such medicinal plant belonging to Asclepiadaceae family and has been widely explored for its medicinal properties. The phytochemical analysis of G.sylvestre revealed the existence of numerous secondary metabolites. Antibacterial activity of acetone extracts of G. sylvestre plant was evaluated, showing resistance to Escherichia coli (Gramnegative bacteria) and Staphylococcus aureus (Gram-positive bacteria). The greater zone of inhibition at the concentration 30µg/mL, against E. coli and S. aureus. Similar results was obtained for minimum inhibitory concentration (MIC) tests, minimum bactericidal concentration (MBC) determination. Estimation of total DNA and total protein levels was performed. Presence of several compounds in the acetone leaf extract was analyzed through GC-MS. Therefore the fraction of G. sylvestre leaf acetone extract evaluated for its antibacterial potential was identified to be an effective antibacterial agent. Keywords: G. sylvestre, Antibacterial activity, MIC, MBC, GC-MS.

[OP-6] Phytochemical Screening and in Vitro Antibacterial Activity of Naravelia zeylanica (L.) against Plant Pathogens Krishnamoorthi, R*1, Natarajan, E2,Vigneswaren, M1 and Sivakumar, S.R1 1

Department of Botany, Bharathidasan University, Tiruchirappalli-24, Tamil Nadu, India. 2

PG and Research Department of Botany, National College (Autonomous), Trichy –01 Email: [email protected].

In the present study we carried out phytochemical analysis and in vitro antibacterial activities of ethylacetate extract of Naravelia zeylanica (L.) Dc. against selected gram positive

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

and gram negative bacteria of plant pathogen. The leaves extracts were studied qualitatively to ascertain the presence of phytochemicals such as coumarin, starch, phenols, tannins, proteins, quinine by adopting standard methods. The antibacterial activities against ten bacterial strains viz., Entrococcus faecium, Staphylococcus aurevs, Methiciltris resistance, Bacillus subtiles, Straptomyces albus, Escherichia coli, Kiebslica pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Legionella pneumophila were tested by adopting agar well disc diffusion

method. Ethyl acetate extract showed antibacterial activity against all the selected bacterial plant pathogens even at minimum concentrations and the highest antibacterial activity recorded in Klebsiella pneumonia (-) (4.57±0.22) in 100 mg/ml. From the result, it is concluded that the

ethyl acetate extract can be used against the tested organisms. Keywords: Naravelia, Bacteria, Pathogens, Phytochemical and Ethylacetate.

[OP-7] Anticancer Potential of RgO-ZnO Nanocomposite Synthesized by Hydrothermal Method using Biogenic Source against Cervical Cancer Mohamed Asik, R1, 2, Suganthy, N3, Thirumurugan, R2, Padmanaban, P4 and Archunan, G1, 2* 1

Centre for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli, India 2 National Center for Alternatives to Animal Experiments, Bharathidasan University, Tiruchirappalli, India 3 Department of Nanoscience and Technology, Alagappa University, Karaikudi, Tamil Nadu, India 4 Lee Kong Chian School of Medicine, Nanyang Technological University, 59 Nanyang Drive, Singapore. Email: [email protected] and [email protected] Biogenic synthesis of nanoparticles from marine sources has gained significant importance, as a simple, cost effective and eco-friendly approach when compared to the conventional methods. The present study was carried out to synthesize reduced graphene oxidezinc oxide nanocomposite (rGO-ZnO) using aqueous extracts of Gracilaria edulis by hydrothermal method and assess its anticancer potential against cervical cancer. Synthesis and characterization of rGO-ZnO NPs were assessed by various analytical techniques, including UVVisible spectroscopy, X-ray diffraction (XRD), Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS) and Fe-SEM. Anticancer efficacy of rGO-ZnO NPS was assessed by MTT assay, apoptotic detection by Acridine orange/ Ethidium bromide dual staining, 9th & 10th February 2018

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

nuclear fragmentation by Hoechst staining, mitochondrial membrane potential by JC-1, DNA damage by comet assay, membrane damage by Annexin V-Cy3-6CFDA dual staining. UV-Vis analysis of GO and ZnO showed a characteristic absorption peak at 229 and 374 nm, corresponding to the GO and ZnO NPs. After the hydrothermal treatment, this peak shifted to 269 and 368 nm, which is assigned to the excitation of π-plasmon of graphitic bond suggesting the reduction of GO and formation of rGO-ZnONP. The diffraction peaks at 31.46°, 34.1°, 35.9°, 47.2°, 56.2°, 62.6° and 67.6° in the XRD patterns of ZnO flower and ZnO/RGO composites are indexed to hexagonal wurtzite ZnO (JCPDS Card 36-1451). Moreover, a broad peak at ∼26.5° can be observed when the higher content of GO was introduced during the preparation process. The observation of this (002) diffraction peak indicates the formation of regular layered RGO sheets as well as the formation of ZnO/RGO composite. Fe-SEM analysis illustrated attachment of ZnO nanoparticles on graphene sheets was observed to be uniform and strong. The surface appeared to be rough, modified and textured, which indicates that ZnO nanoparticles have been attached on surface of grapheme nanosheets. A strong peak arising at 632 cm−1 in FTIR analysis is the characteristic of stretching vibration in ZnO nanoparticles.

Results of MTT assay

illustrated dose-dependent cytotoxic effect of rGO- ZnO NPs against cervical cancer cell line SiHa with IC50 value of 40±0.5 µg/ml. ZnO NPs exhibits anticancer effect by activating ROS mediated oxidative stress, disrupting mitochondrial membrane potential leading to activation of caspase mediated apoptosis, disrupting the cell membrane integrity and inducing DNA damage. Results of present study illustrate that rGO-ZnO nanocomposite synthesized through biogenic route exhibits potent anticancer effect against cervical cancer cells via induction of apoptosis and cell cycle arrest, which might provide new insight for future development of novel nanotherapeutic molecules for the treatment of cervical cancers. Keywords: Hydrothermal method; Gracilaria edulis; rGO-ZnO nanocomposite; SiHa cells; cytotoxicity; Reactive oxygen species; Mitochondrial membrane potential; DNA damage.

[OP-8] A Study on Isolation and Culturing of Mesenchymal Stem Cells from the Omental Tissue Cecily Rosemary Latha, R* and Jemi Feiona, V.A. PG and Research Department of Zoology, Holy Cross College, Trichy. Email: [email protected]

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International Conference on “Molecular Advances in Biomedical Research – (ICMABR-18)

Diseases now-a-days have become more challengeable to treat and cure. However conventional treatment methods fail to respond to end stage diseases. In recent decade’s usage of stem cells have shown good results though they have been used as a last line treatment.Stem cells are used as resource in regenerative medicine as replacement therapy which has been a new pillar for human therapeutics. Thus the stem cells take a separate and vital place in the research, medical and clinical fields. In this study the mesenchymal stem cells (MSCs) have been isolated from the omental fat tissue. Since omental fat tissue act as an abundant source for mesenchymal stem cells, it can be harvested in large numbers with low donor-site morbidity by elective liposuction. In this study MSCs have been isolated from stromal vascular fraction (svf) of digested omental fat using collagenase, and then the MSCs are cultured and observed regularly to check their health, contamination and confluency. Once it reaches 80% confluence the cells are passaged in the ratio of 1:4 in a T75 flask and maintained. The cells were sub cultured till passage two and have shown good growth result without any alterations. After reaching the confluence in passage two the MSCs have been cryopreserved in the cryovials in liquid nitrogen for future potential use. The stored vial(s) can then be thawed as and when needed and used for clinical applications, to treat foot ulcers, certain nerve disorders, diabetes mellitus, bone defects, cartilage repair, liver disorder, vascular disorder and many other diseases due to its antiinflammatory and immunomodulatory properties.

[OP-9] Salivary Metabolomics Screening of Putative Biomarkers during early Diagnosis of Alzheimer's Disease Alagendran, S1, 3, Pushpa, N2 and Archunan, G3 1

Department of Biochemistry, Crop Improvement Division, Adhiyamaan College of Agriculture and Research, Krishnagiri - 635 105 2 PG & Research Department of Microbiology, Cauvery College for Women, Trichy- 620 018 3 Centre for Pheromone Technology, Department of Animal Science, School of Life Sciences, Bharathidasan University, Trichy - 620 024. Mild cognitive impairment (MCI) imparts an increased prospect of rising Alzheimer's disease (AD). There is high interest in the discovery of early diagnostic biomarkers that could predict MCI to AD progression, for which saliva metabolomics exhibits a great potential. In this work ELISA was developed to examine metabolic differences of acetylcholine and esterase activities in saliva samples from MCI subjects and age-matched AD subjects; multivariate analyses were used to define the differences between MCI and AD groups; and receiver 9th & 10th February 2018

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operating characteristic analysis shows the sensitivity (94.8%) and specificity (96.6%), indicating potential diagnosis in the AD.

[OP-10] Analysis of Salivary Organic Acid in Buffalo with Reference to Estrus Srinivasan Mahalingam1, Salha Maheen2, Kalaivani1, Saranya Uruthirapathy2, Nargis Begum Tajuddin2, Padmanabhan, P3 and Govindaraju Archunan1* 1

Centre for Pheromone Technology, Department of Animal Science, Bharathidasan University, Tiruchirappalli, Tamilnadu -620024. India. 2 Department of Biotechnology, Jamal Mohamed College, Tiruchirappalli-620020, Tamilnadu, India. 3 Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore. Email: [email protected] Buffaloes are the main milk producing unit and the backbone of farmers, dairy and meat

industries. The estrus behaviour is less intense than that of bovine and hence, it is consequently much more difficult to detect and they are identified as silent heat. Organic compounds like ascorbic acid, acetic acid and propionic acid are identified in farm animals. Though saliva is considered as the best source of biological material and it is noninvasive in comparison to other body sources. The present investigation was aimed to analyse the salivary organic acids using High Performance Liquid Chromatography (HPLC). Reproductively healthy buffaloes were chosen and saliva samples were collected during different phases of estrous cycle i.e., pre-estrus, estrus and di-estrus. Sample were subjected to HPLC analysis for conformation of the organic acids. Among the different peak pattern across the estrous cycle of the buffalo observed in HPLC analysis revealed the presence of organic acids in saliva. However, the organic acids (ascorbic and acetic acid) concentration are varied depend upon the reproductive phases of buffalo, however the propionic acid is not all observed in saliva. The significant concentration of ascorbic acid and acetic acid during the estrus phase was (P˂0.05) high when compared with pre-estrus and di-estrus phases. At the same, when compared with pre-estrus and di-estrus the rate of ascorbic acid and acetic acid is high in pre-estrus. Throughout the comparison of these acids between all the phases of estrous cycle the ascorbic acid found high during estrus phase. Overall the results conclude that rate of ascorbic acid and acetic acid are consider as estrus indicator in buffalo saliva. Keywords: estrus, buffalo, acetic acid, ascorbic acid, HPLC, saliva.

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[OP-11] Extracellular Polymeric Substances from Microalgae and Their Biotechnological Potential Saranya Uruthirapathy1, Salha Maheen1, Nargis Begum Tajuddin1*, Shilu Mathew2, Thajuddin, N3 and Muhammad Ilyas4 1

Department of Biotechnology, Jamal Mohamed College, Tiruchirappalli-620020, Tamil Nadu, India. 2 Biomedical Research Centre, Qatar University, Doha, Qatar. 3 Department of Microbiology, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India. 4 Department of Bioinformatics, School of Life Sciences, Bharathidasan University, Tiruchirappalli-620 024, Tamil Nadu, India. Email: [email protected]. Extracellular polymeric substances (EPSs) are natural polymers of high molecular weight secreted by microorganisms into their environment. They are mostly composed of polysaccharides (exopolysaccharides) and proteins, but also include other macro-molecules such as DNA, lipids and humic substances. EPSs are the construction material of bacterial settlements and either remains attached to the cell's outer surface, or are secreted into its growth medium. These compounds are important in biofilm formation and cells attachment to surfaces. EPSs constitute 50% to 90% of a biofilm's total organic matter. Microalgae have been studied as natural resource for various applications, most especially sustenance, animal feed, biofuels, pharmaceuticals, and nutraceuticals. In addition to the intracellular compounds of interest, microalgae can likewise excrete different extracellular polymeric substances (EPS) are the exopolysaccharides into their prompt living condition during their life cycle to frame a hydrated biofilm matrix. The fundamental barriers to the industrial application of microalgae for biofuel production still include high costs of removing the algae from the water and the water from the algae which can account for up to 30–40% of the total cost of biodiesel production. Algal biofilms are becoming increasingly popular as a strategy for the concentration of microalgae, making harvesting/dewatering easier and cheaper. Most remarkably, EPS hold their steady matrix structure and form a 3-D polymer organize for cells to interact with each other, and mediate their grip to surfaces. EPS also play a role as extracellular vitality and carbon sinks. They are also abundant source of structurally and compositionally diverse biopolymers which possess remarkable bioactivities for extraordinary high-value applications, particularly as antivirals, antitumor agents, antioxidants, anticoagulants or antithrombotic, immunomodulatory 9th & 10th February 2018

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ability, hypoglycaemic, antibiotics, and hostile to inflammatories. Their prevalent rheological properties additionally make microalgal EPS especially valuable in mechanical engineering (e.g., biolubricants and drag reducers) and food science (e.g., thickener and additives) applications. The significance of exopolysaccharides and EPS proteins, with their specific metabolic attributes, are likewise depicted as a result of their potential high-esteem applications.Their properties are mainly due to their structure and physicochemical characteristics, which depend on the organism they are produced by. In the biomedical field, the polysaccharides from algae can be used in controlled drug delivery, wound management, and regenerative medicine. Keywords: EPSs, Biofilm, Pharmaceuticals, Nutraceuticals, Biofuel, Biopolymers, Rheological.

[OP-12] Surfactant Induced Skeletal Anomalies in Puntius parrah Larvae Rini Raphael1 and Umamaheswari Sepperumal2 1

Dept. of Zoology, Carmel College, Mala, Thrissur, Kerala Email: [email protected] 2 PG and Research Department of Zoology, Periyar EVR College, Tiruchirappalli – 620 023, Tamil Nadu, India Email: [email protected] Earlier developmental stages of fishes are more sensitive to fluctuations in the environment than the adult and they can be considered as an effective bio tool to assess pollution in the aquatic system. Several chemical water pollutants are capable of inducing notochordal damage in larvae, and can interfere with the normal development. In this study, newly hatched larvae of Puntius parrah were exposed to different concentrations of Sodium Dodecyl Sulphate, an anionic surfactant and were examined after 72 hours hatching. A significant higher number of Puntius parrah larvae exposed to Sodium Dodecyl Sulphate were detected with skeletal

malformations and severity of the abnormality was highest at the highest concentration of SDS. The major skeletal malformations observed in the present investigation include scoliosis, lordosis, end tail anomalies and abnormal jaw formations. As far as fisheries sector is concerned, skeletal deformities is a major factor that affects the production cost, the external morphology of the fish as well as its survival and growth. The present observations reveal the teratogenic effect of Sodium Dodecyl Sulphate on fishes. It is recommended that all kinds of wastewaters, sewage and agricultural wastes must be treated before discharging into the aquatic systems.

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[OP-13] Utilisation of Polyethylene Terephthalate for Production of Cellulose by Bacillus Licheniformis Umamaheswari, S* and Puspavalli, M. P.G and Research Department of Zoology Periyar EVR College, Tiruchirappalli, Tamil Nadu- 620 023. *Email: [email protected], Mobile: 91-9443411627 Cellulose has a wide application in food paper making industry, medical and electronic goods. This research work primarily focuses on tapping the potential of bacteria to produce cellulose, which could be helpful to develop a protocol to produce bacterial cellulose at a large scale. At the same time, the accumulation of synthetic polymeric wastes like PET is of environmental concern. If the bacteria are able to utilize PET as a carbon source and produce cellulose, the twin problem of accelerating demand for cellulose and load of synthetic polymeric PET wastes could to be reduced. The demand for cellulose in paper making industry is escalating. Thus alternate source of cellulose could be a better option for meeting this demand. With this aim, the present study was designed to evaluate the potential of cellulose production by soil bacteria using polyethylene terephthalate as a carbon source. The results indicate that Bacillus licheniformis isolated from soil has the potential to produce cellulose by using PET as a

carbon source. This could in turn reduce the PET waste accumulation and depletion of forest in the environment. Yet, a viable protocol has to be developed for optimal production of BC using PET.

[OP-14] RAPD Profiling of Indigenous Earthworm Resources of Sivagangai District. Arockia John Paul, J1*, Karunai Selvi, B2, Biruntha, M3 and Archana, J3 Dept. of Zoology, Arumugam Pillai Seethai Ammal College, Tiruppattur– 630 211. 2 Department of Botany, V.V. Vanniaperumal College for Women, Virudhunagar – 626 001. 3 Department of Animal Health and Management, Alagappa University, Karaikudi – 630 003. Email: [email protected] 1

Nine different species of earthworms belonging to three families and seven genera have been reported. Vermicomposting potential of locally abundant earthworm species showed that Amynthas sp. was able to produce vermicompost efficiently followed by Perionyx excavatus, Lampito mauritiiand Drawida pellucidapallida. RAPD analysis was performed to identify the

level of genetic variation and polymorphism in Lampito mauritii collected from ten different

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locations and Drawida pellucidapallida collected from nine different locations. The genetic similarities between the earthworms Lampito mauritti and Drawida pellucidapallidacan be identified by comparing the banding patterns DNA barcoding has highlighted the expanding use of the mtDNA gene, cytochrome oxidase I (COI), as a genetic marker for species identification. Cytochrome oxidase I (COI) sequencing was performed in the newly identified earthworm species Amynthas sp. Cytochrome oxidase I (COI) sequencing was performed in the newly identified earthworm species Amynthas sp. JPA-2017 isolate APSAC E2 and deposited in GEN BANK of NCBI (Accession number: KY886135). Highly efficient vermicomposting earthworm, Amynthas sp. were reported and domesticated successfully.

Keywords: Earthworm, RAPD analysis, DNA barcoding, COI Sequence.

[OP-15] Biosynthesis and Characterization of Red Pigment from Monascus purpureus and its Antioxidant and Antitumor Property Gayathri, M.V, Josephin Jancy Rani, P and Avila Jerley, A* PG & Research Department of Zoology, Holy Cross College, Tiruchirappalli – 620002 Email: [email protected] Mobile: 9788662011 The fruiting bodies of some macromycetes and sporocarp are used as sources of pigments. Fungal pigments that are generally biosynthesized during their metabolism are belong to polyketides groups.Filamentous fungi also synthesize various natural pigments which have ecological functions and are of value to the producer. Among them the fungi Monascus purpures is unique in nature which produces many secondary metabolites. In this paper the studies were focussed on Solid-state fermentation which was carried out using rice as a substrate for the production of pigments using a fungal culture of Monascus purpureus MTCC 1090. The pigment complex was extracted with solvent system and purified by column chromatography and TLC to obtain the red pigment fraction, monascorubramine. The spectrophotometric analysis revealed λmax at 500 nm. FT-IR & NMR analysis was done to identify their functional groups, which

confirms their structure. Microbial pigments are highly responsible for the health benefits and play a key role as antioxidants due to the presence of hydroxyl substituent and their aromatic structures which enables them to scavenge free radicals which may lead to cancer. These antioxidants interact and stabilize free radicals which prevent some of the damages caused by 9th & 10th February 2018

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them.Here in this research the antioxidant activity of the RYR was carried out by free radical scavenging assay and this red pigment showed the presence of antioxidant potency which is one of the important health components for functional foods. Further they are analysed for antitumor activity tested against HEp-2 cell lines by cell cytotoxicity assay and by MTT assay. The morphological changes in cells and 15,02% of cytotoxicity confirms that pigment from fermented rice of Monascus purpureus has effective anti-tumour property. Hence, microbial pigments have therapeutic ability and can provide a novel material for human health concern.

[OP-16] Extraction and Evaluation of Antimicrobial and Hemolytic Activity of the Coelomic Fluid of Lampito mauritii and Examination of its Biomolecules using GC-MS Analysis. Uma Mageshwari, P and Bhorgin Lourdu Mary, A. J* PG and Research Department of Zoology, Holy Cross College, Trichy. Email: [email protected] Mobile: +91-9487358444 Earthworms and their products have been used as a potential drug for the treatment of various diseases. Coelomocytes and humoral immune proteins, both components of the earthworm coelomic fluid helps to survive in soil with different pathogenic microorganism. In earthworm, coelomocytes serves the major immune response that provides defense mechanism against the disease causing micro-organisms. Coelomic fluid from earthworm Lampito mauritii were collected and evaluated for antimicrobial and hemolytic activity, also examined for the presence of biomolecules using gas chromatography- mass spectrometry. The results of antimicrobial activity confirmed that the coelomicfluid has positive effect on Staphylococcus aureus, Klebsiella pneumonia, Salmonella typhi, E. coli, Pseudomonas sp., and Aspergillus niger. The gas chromatography- mass spectrometry analysis revealed that the presence of several

biomolecules incoelomic fluid. Molecular docking of the identified compounds with microbial enzyme autolysin confirms that coelomic fluid prevents infection by binding to pathogenic moiety. The immune response of earthworm might be due to the presence of biomolecules and the protein component in the coelomic fluid. Key words: Lampito mauritii, antimicrobial, hemolytic, coelomic fluid.

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[OP-17] Assessment of Radiation Dose by Biodosimetry Senthamil Selvan, C Department of Medical Physics, Bharathiar University, Coimbatore-641 046, Tamil Nadu. Email: [email protected]. Ionizing radiation has many practical uses in medicine, research, and other areas, it presents a health hazard if used improperly. Biological dosimetry is the measurement of radiation induced changes in the human body to assess acute and long term health risks. Irradiation of 10 ml blood sample undergone few steps to analyze laboratory data mainly which involve Cells cultures and lymphocytes study. Exposures over 1 Gray the patients are monitored by healthcare professions and followed International Atomic Energy Agency (IAEA) recommendation values mentioned in technical report no. 260 and 405. Nationally nuclear activity used in all disciplinary, monitoring and safety bodies of Atomic Energy Regulatory Board (AERB), India, all across the country for biodosimetry. Biological dose estimation provides an independent means of obtaining dose information otherwise exclusively based on computer modeling, dose reconstruction, and physical dosimetry.Need for biodosimetry for initial triage for military forces and civilian populations when there are radiation exposures that involve potentially a large number of persons. It gives several advantages are premature chromosome condensation which enables analysis in interphase, simplicity to count lymphocytes, measures actual cellular damage rather than dose received and most useful when compare with other types of dosimetry.Based on this to increase number of the facility and develop data on cytogenetic, somatic mutation, and gene expression with help of biodosimetry.

[OP-18] Anticancer Potential of Glibenclamide: It’s Synergistic Cytotoxicity with Doxorubicin on Liver Cancer Cells Nithyananthan, S and Thirunavukkarasu, C Department of Biochemistry and Molecular Biology, Pondicherry University, Puducherry 605 014, India. Email: [email protected]; [email protected] Drug repurposing has been an emerging therapeutic strategy, which involves exploration of a new therapeutic approach for the use of an existing drug. Glibenclamide (Gli) is an antidiabetic sulfonylurea drug extensively used for the treatment of type-2 diabetes, it has also been

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shown to possess anti proliferative effect against several types of tumors. The present study was executed to understand the mechanisms underlying the interaction of Gli with DNA under physiological conditions. The binding mechanism of Gli with DNA was scrutinized by UVvisible absorption spectroscopy and fluorescence emission spectroscopy. The conformational changes and electrochemical properties were analyzed by circular dichroism spectroscopy and cyclic voltammetry. Isothermal titration calorimetry was employed to examine thermodynamic changes and molecular docking technique used to analyze the interaction mode of Gli with DNA. The spectroscopic studies revealed that Gli interact with DNA through groove binding mode, further isothermal titration calorimetry depicted a stronger mode of interaction favorably groovebinding. Recently systemic combination therapy has shown significant promise in inhibiting multiple targets simultaneously yielding high therapeutic competence with lesser side effects. In this concern we intended to study the combined cytotoxicity of Gli with doxorubicin (Dox). The results of MTT assay and acridine orange (AO) /ethidium bromide (EtBr) staining showed synergistic cytotoxicity of Gli-Dox combination on HepG2 & MCF-7 cells. The present study documents the intricate mechanism of Gli–DNA interaction and delivers a multifaceted access for chemotherapy by Gli-Dox combination. Keywords: Glibenclamide; DNA interaction; grove binding; Combination therapy; Anti-cancer.

[OP-19] Antidiabetic Effect of Ethanolic Leaves Extract of Azima tetracantha Lam. in Streptozotocin Induced Diabetic Rats Josephinol, S1, Nargis Begum, T2*, Muhammad Ilyas, M .H3, Shilu Mathew4, Aakila Shahul2 and Neeraja Anandamyle2 1

Department of Biochemistry, Holy Cross College, Tiruchirappalli-620 002, Tamil Nadu. 2 Department of Biotechnology, Jamal Mohamed College, Tiruchirappalli-620 020. 3 Department of Bioinformatics, Bharathidasan University, Tiruchirappalli-620024, 4 Biomedical Research Centre, Qatar University, Doha, Qatar. Email: [email protected] The goal of the present studywas to investigate anti-diabetic activity of ethanolic leaves

extract of Azima tetracantha Lam. in streptozotocin induced diabetic rats.At a regular interval of an experimental protocol blood glucose, insulin, glycogen, High-density lipoprotein (HDL), Low-density lipoprotein (LDL), very low density lipoprotein (VLDL), Free fatty acid (FFA), phospolipid (PL) and total cholesterol (TC) and body weight proportion were assessed in streptozotocin induced diabetic rats. Histology of pancreas and liver was also studied. The 9th & 10th February 2018

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statistical analysis of streptozotocin induced diabetic rats were observed in accordance to 4, 15, 30, 45, 60 days. A significant decrease in blood glucose and significant increase in insulin and glycogen were observed in diabetic rats treated with A. tetracantha Lam. treatment resulted in significant reduction of LDL, VLDL, FFA, TC while increased HDL content is observed. Histology of diabetic rats treated with A. tetracantha Lam. showed the pancreatic β-cells regeneration. These findings suggest that A.tetracantha Lam. has potent antidiabetic activity in streptozotocin induced diabetic rats. Keywords: Azima tetracantha Lam., Antidiabetic, Streptozotocin, Histology, Insulin

[OP-20] Isolation and Screening of Plant Growth Promoting Rhizobacteria from Tomato Rhizosphere Soil Kalaivani, S1, 2, Muthu Selvam, R1 and Chandru, S1* 1 Department of Microbiology, Winro Research Institute of Biological Sciences, Tiruchirappalli - 620007. Tamil Nadu, India. 2 Department of Biotechnology, Pavendar Bharathidasan College of Engineering & Technology, Tiruchirappalli - 620 024.Tamil Nadu, India. Email: [email protected] Plant growth promoting rhizobacteria (PGPR) are the rhizosphere bacteria that can enhance the plant growth by a wide variety of mechanisms. They help in providing free living nitrogen fixing bacteria, increase supply of other nutrients, produce plant hormones, enhance other beneficial bacteria or fungi, control bacterial and fungal diseases and help in controlling insect pest. The present study deals with isolation and characterization of PGPR were isolated from tomato rhizosphere soil. The rhizosphere soil was collected from tomato plant growing sites in Ayyalur, Dindigul and the physico-chemical and biological properties were analysed from the rhizosphere soils. Initially, total offour difference type bacterial strains were isolated from the rhizosphere soil. The two PGPR isolates were selected based on the indole acetic acid, siderophore production and phosphate solubilization activity. PGPR strains TM1 and TM3 produced maximum amount of indole acetic acid and siderophore. TM3 strain was showed maximum number of phosphate solubilizing (11mm). On the basis of morphological, biochemical analysis has revealed that, the PGPR isolates were authentically identified as Pseudomonas sp. (TM1) and Bacillus sp. (TM3). Bacterium provides benefits to the plant,

resulting in plant growth stimulation, plant protection as well as production of antibiotics, geochemical cycling of minerals and plant colonization.

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[OP-21] Influence of Parity Status on Urinary Protein Profile and its Dynamics in Murrah Buffalo Kasanraj, G1,2 , Prithivrajan, A1, 2, Muthu Selvam, R1, 3* and Archunan, G3* 1

Department of Biotechnology, Winro Research Institute of Biological Sciences, Tiruchirappalli. Department of Biotechnology, Pavendar Bharathidasan College of Engineering & Technology, Tiruchirappalli. 3 Department of Animal Science, Bharathidasan University, Tiruchirappalli. Email: [email protected]; [email protected]

2

Estrus prediction is difficult in murrah buffalo which affects the dairy industry based economy of the nation. Several attempts have been made for decades but no cost-effective method is developed till date for estrus detection in this animals. Luteinizing hormone detection based estrus detection method has been proposed but the accuracy of detection is un-satisfactory. However, the sensitivity of the kit might be increased when urinary proteins including common peptides and phase specific urinary peptides were used as auxiliary markers.Hence, parity specific variation in the urinary protein has to be investigatedin terms of profile and quantity of specific peptides.Reproductively healthy female buffaloes were recruited for the present study and animals were segregated based on its parity status as nulliparous, primiparous and multiparous. Mid-stream urine samples were collected and urinary proteins were concentrated using cut-off membrane centrifugal column and various organic solvents. The urinary proteins were separated in polyacrylamide gel-matrix. Protein profile variation was analysed and the quantity of each bands were estimated using software. The urinary proteins without any parity specific variation will be consider as a common peptide for the development of estrus specific kit as an auxiliary marker. Keywords: Estrus, Murrah Buffalo, leuteinizinng hormone, Urine, Peptide

[OP-22] Observation of Courtship Behaviors in White Yorkshire Pig in Reference to the Substantiation of Estrus Phase Silambarasan V, Deepalakshmi G and Archunan G* School of Life Sciences, Bharathidasan University, Tiruchirappalli, Tamilnadu, India. *Email - [email protected]

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Sexual behaviors in animalswere studied by biologists,and ethologists in the management of wildlife resources for welfare of animals whereas other studiesinvolved in analyzing hormone and pheromone actions.Courtship behavior is a set of animal behaviors exposed in order to perform pre-copulatory selection. It is done as means of communication for expressing the desire to mate with opposite partner. Sus scrofa domesticus, a breed of white Yorkshire has been widely used by developing and developed nations. Pig was also utilized for developing commercial food products, organic manure, leather materials etc. The procreation in swine is seen to be very low at its success level. A variety of human and environmental factors, in addition to the sow’s own genetic potential, affect the procreation efficiency, farrowing success and litter size. Artificial insemination (AI) can overcome such impediments. The success rate of AI procedure depends upon detection of estrus stage in sow which may be done by observing certain behavioral changes expressed by animal but confirmation was carried out by canvassing the courtship behavioral responses. Sniffing, mounting, hyper salivation are the major courtship behavioral responses that are elicited by boar owing to the exposure of various bodily fluids from different stages of sow.The behavioral response exhibited by boar is found to vary towards the different reproductive stages of sow. Understanding the courtship behavioral responses aids in identification of major pheromone source. This may pave a way for further pheromonal analysis. Keywords: Courtship behavior, estrus, hyper salivation, artificial insemination, pig.

[OP-23] Novel Triterpenoids from Cassia fistula l. Regulate P53 and ERK2 Genes to Induce Apoptosis in HT-29 Colon Cancer Cells Anisha Marina, M1, Rajini, M. Kumar2, Jeyapriyadharshini1, Ludas, A2, Indu, S2, Anita Roy, A2 and Rajalakshmi, M1, 2* 1

2

PG and Research Department of Zoology, Holy Cross College (Autonomous), PG and Research Department of Biotechnology & Bioinformatics, Holy Cross College (Autonomous), Tiruchirappalli, Tamilnadu, India. Email: [email protected]

Incidence of colon cancer is on a rise in the current decade. Modern means of medication to tackle the disease yet suffer from several side effects, and an alternative means, to reduce the risk phytotherapeutics, is of contemporary focus. Our study explored, adopting in-vitro and insilico approaches, the anticancer efficacy of three novel triterpenoids derived from ethyl acetate

extract of Cassia fistula L., against human colon cancer cell line (HT-29). Two of the triterpenoids were found to be non-toxic on normal VERO cells and at the same time

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demonstrated selective cytotoxicity to cancer cells where in the IC50 concentrations were 50 µM and 40 µM, respectively, bringing up apoptotic features such as chromatin condensation, DNA fragmentation, membrane leakage and enhanced depolarization of mitochondrial membrane, with subsequent cell cycle arrest at S-phase and G2/M phase, respectively. RT-PCR analysis revealed that both the compounds produced upregulation of p53 expression and downregulation of ERK2 expression. The rates of invasion and metastasis were significantly decreased on exposure to the triterpenoids. Furthermore, enhanced catalase activity was evident on destruction of cancer cells. Docking results showed a stable interaction between the triterpenoids and the cancer targets (ERK2 & p53). The data suggest that both the triterpenoids exhibit high antiproliferative effect in HT-29 cells and are promising as alternative cancer therapeutics. Keywords: Cassia fistula L., colon cancer, antioxidant activity, triterpenoids, p53, ERK2.

[OP-24] In Vitro and In Silico Antioxidant and Hepatoprotective Potential of Pouteria campechiana Thilagameena, S1, Smilin Bell Aseervatham, G1* and Sivasudha, T2 1

PG Research Department of Biotechnology and Bioinformatics, Holy Cross College (Autonomous), Tiruchirappalli 620024, Tamilnadu, India 2 Department of Environmental Biotechnology, Bharathidasan University, Tiruchirappalli 620024, Tamilnadu, India The current research aims to analyze the antioxidant and hepatoprotective effect of P. campechiana fruit extract against acetaminophen intoxicated rats. Pouteria campechiana

(Kunth) Baehni is a significant remedy for coronary trouble, liver disorders, epilepsy, skin disease and ulcer. P. campechiana fruit extract effectively quenched the free radicals in a concentration-dependent manner within the range of the given concentrations in all antioxidant models. The presence of polyphenolic compounds were confirmed through UPLC-MS/MS and the following compounds were identified (ginsenoside, naringenin, chrysoeriol 8-c-glucoside, luteolin-6-C-glucoside, apigenin-6,8-di-C-β-D-glucopyranoside). Hepatoprotective activity of PCAE was analyzed with rat as animal model. The animals were treated with acetaminophen (250mg/kg body weight; p.o.) thrice at the interval of every 5 days after the administration of P. campechiana aqueous extract and silymarin (50 mg/kg). An oxidative stress in liver which

leads to an increase of serum marker enzymes was triggered by the administration of acetaminophen treatment which has been founded. However, treatment with P. campechiana 9th & 10th February 2018

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fruit extract significantly reduced the prominent liver marker enzymes (aspartate transaminase, alanine transaminase, and alkaline phosphatase) and increased the antioxidant enzymes (viz., superoxide dismutase and catalase). In the same manner, glutathione indicating the effect of the extract in restoring the normal functional ability of hepatocytes. Molecular docking studies of the selected compounds from P. campechiana into CYP4502E1 and ACC1 binding site revealed a clear preference for the binding energy and H-bond interaction. These results strongly suggest that P. campechiana fruit extract has strong antioxidant and significant hepatoprotective effect against acetaminophen induced hepatotoxicity

[OP-25] Anti- steriodogenic and withdrawal effect of Thespesia populnea (L.) on female genital tract in mice Chandru, G¹* and Sethupathy, S ¹Department of Zoology, A.V.C. College (Autonomous), Mannampandal - 609 305, Tamil Nadu, India 2 Department of Biochemistry, RMMC & H., Annamalai University, Annamalai nagar-608 002, Tamil Nadu, India. Email: [email protected] Phone: 91-9994820962 Thespesia populnea Corr. floral extract has been shown to have anti-steroidogenic effect.

Bark extract of Thespesia populnea has no toxicity as per the earlier reports of traditional medicine used by the tribal peoples of Indira Gandhi Wild Life Sanctuary, Pollachi, South India. In the present study, anti-steroidogenic effect of Thespesia populnea (Malvacea) bark was evaluated in adult female mice (Mus musculus). Methanolic extract of Thespesia populnea bark (METP) was administered orally at the doses of 100, 250, 400 mg/kg body weight for a duration of 60 days, and normal saline (0.9%) to control group. There was significant (P