Monoclonal Antibodies to Human Plasma Low-Density Lipoproteins. II ...
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Monoclonal Antibodies to Human Plasma Low-Density Lipoproteins. II ...
We describe the importance of the low-density lipoproteins. (LDL) used in preparing radioimmunoassay standard curves and the clinical application of ...
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Monoclonal Antibodies to Human Plasma Low-Density Lipoproteins. II. Evaluation for Use in Radioimmunoassay for Apolipoprotein B in Patients with Coronary Artery Disease James G. Patton, Juan-Jos#{233} Badimon, and Simon J. T. Mao1 We describe the importance of the low-density lipoproteins (LDL) used in preparing radioimmunoassay standard curves and the clinical application of monoclonal antibodies to LDL. LOL isolated from five normal men produced five parallel displacement curves with conventional mouse antiserum but there was a significant difference of immunoreactivity among the LOL. None of our four monoclonal antibodies could eliminate the heterogeneous immunoreactivity of different LDL. Thus, the determination of plasma apolipoprotein (apo) B will vary depending on the selection of LDL standards, and the comparison of absolute ape B values between laboratones will be of questionable value unless they use the same LDL standard. Nonetheless, in a radioimmunoassay our monoclonal antibody, LP-22, detected a more significant (p 0.3), greater than the concentrations determined with the conventional antibody method (x). The
ape
Table 2. Apo B Concentrations (mean ± SD) in 10 Normolipemic and 10 Hypertriglyceridemic Subjects Concn, g/L Hypertri-
