Nahmias Yaakov, Ph.D.

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Nahmias Yaakov, Ph.D. The Selim and Rachel Benin School of Computer Science & Engineering. The Alexander Silberman Institute of Life Sciences.
Nahmias Yaakov, Ph.D. The Selim and Rachel Benin School of Computer Science & Engineering The Alexander Silberman Institute of Life Sciences Department of Cell and Developmental Biology The Hebrew University of Jerusalem Head of Bioengineering Program Director, Center for Bioengineering Website: http://www.cs.huji.ac.il/~ynahmias Cell: (054) 610 4088 Office: (02) 658 4406 Fax: (02) 658 5261 E-mail: [email protected] Center for Engineering in Medicine Massachusetts General Hospital Ph: (617) 398 3442 E-mail: [email protected]

Research Statement My work lies at the intersection of tissue engineering, microfabrication, and systems biology. My group is focused on the development of high throughput microfabricated platforms for the study of liver development, regeneration, and metabolism. We apply the tools we develop for Metabolic Reprogramming: understanding how liver cells process information and make metabolic decisions. Our goal is to control patient’s metabolism using this approach. We look toward the development of the next generation of drugs for the treatment of cancer, viral infection, as well as metabolic diseases such as diabetes, obesity, and atherosclerosis.

Education 1995-1999

B.Sc.

2000-2004

Ph.D.

2004

Postdoc

2004-2006

Postdoc

Technion, Israel Institute of Technology Haifa, Israel Chemical Engineering, Biology (Magna Cum Laude) University of Minnesota Minneapolis, MN Biomedical Engineering, Scientific Computation University of Minnesota Minneapolis, MN Biomedical Engineering Harvard Medical School Boston, MA Massachusetts General Hospital (MGH)

Professional Experience 2010-current 2010-current 2009-current 2006-current 2007-2009 2004-2006 2004-2006

Director, Center for Bioengineering, Hebrew University of Jerusalem Head, Bioengineering Program, Hebrew University of Jerusalem Senior Lecturer, Bioengineering Department, Hebrew University of Jerusalem Instructor in Surgery and Bioengineering, Harvard Medical School Scientific Staff, Shriners Burns Hospital, Boston, MA Research Associate, Department of Surgery, Massachusetts General Hospital Fellow, Shriners Burns Hospital, Boston, MA

2004 2000-2001 1999-2004 1997-1999 1996

Research Associate, Biomedical Engineering, University of Minnesota Teaching Assistant, Biomedical Engineering, University of Minnesota Research Assistant, Biomedical Engineering, University of Minnesota Research Assistant, Food Engineering and Biotechnology, Technion Quality Control, Carmel-Mizrahi Winery, Rishon Le-Zion, Israel

Honors and Awards Chair, 3rd Methods in Bioengineering Conference, Harvard Medical School (2011) Member of the European Research Council LS9 Review Panel (2011) Golda Meir Fellow (2010) Young Investigator Award, Cell Transplant Society (2009) NIH Research Scientist Career Award (2008) Chair, 2nd Annual Methods in Bioengineering Conference, MIT (2007) Shriners Burns Institute, Postdoctoral Fellowship (2006) Chair, 1st Annual Methods in Bioengineering Conference, MIT (2006) BMES Annual Fall Meeting, Nashville, Presentation Award (2003) BMEI Tissue Engineering Interest Group, Doctoral Fellowship (2003) Medical Alley, Dean of Medical School Award (2003) BMEI Doctoral Fellowship (2002) Medical Alley, BMEI Director Award (2002) BMES Student Chapter, Annual Poster Symposium, Best Poster (2001) BMES Annual Poster Symposium, First year category, Best Poster (2000) UMN International Student Scholarship (1999) Department of Chemical Engineering, Dean Award of Excellence (1996-1999) Technion, Israel Institute of Technology, Presidential Award of Excellence (1998)

Dissertation Y. Nahmias. Laser-guided direct writing for three-dimensional tissue engineering: Analysis and application of radiation forces. PhD Thesis, University of Minnesota, 2004.

Publications 1. Y. Nahmias and D. J. Odde, Analysis of Radiation Forces in Laser Trapping and Laser-Guided Direct Writing Applications, IEEE Journal of Quantum Electronics, 38:131-141 (2002) 2. Y. Nahmias, B.Z. Gao, and D. J. Odde, Dimensionless Parameters for the Design of Optical Traps and Laser Guidance Systems, Applied Optics, 43(20):3999-4006 (2004) 3. Y. Nahmias, A. Arneja, T. Tower, M. J. Renn, and D. J. Odde, Cell Patterning on Biological Gels via Cell Spraying Through a Mask, Tissue Engineering, 5/6 (11): 701-708 (2005). 4. Y. Nahmias, R. Schwartz, C. Verfaillie, and D. J. Odde, Laser guided direct writing for threedimensional tissue engineering, Biotechnology and Bioengineering, 92(2):129-136 (2005). 5. Y. Nahmias, R. Schwartz, W-S. Hu, C. Verfaillie, and D. J. Odde, Endothelium-mediated hepatocyte recruitment in the establishment of liver-like tissue in vitro, Tissue Engineering 2006;12(6):1627-1638 6. Y. Nahmias, M. Casali, L. Barbe, F. Berthiaume, M. L. Yarmush, Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary hepatocytes, Hepatology, 43(2):257-265 (2006).

7. Y. Nahmias, I. Kramvis, L. Barbe, M. Casali, F. Berthiaume, M. L. Yarmush, A novel formulation of oxygen carrying-matrix enhances liver specific function of cultured hepatocytes, FASEB J., 20(14):2531-2533 (2006) 8. Y. Nahmias, D. J. Odde, Micropatterning of living cells by laser-guided direct writing: application to fabrication of hepatic-endothelial sinusoid-like structures, Nature Protocols, 1(5):2288-2296(2006) 9. C. H. Cho, N. Parashurama, E. Y.H. Park, K. Suganuma, Y. Nahmias, F. Berthiaume, A. W. Tilles, M. L. Yarmush, Homogenous differentiation of hepatocyte-like cells from ES cells, FASEB J. 22(3):898-909 (2008) 10. Q. Song, R. Risco, M. Latina, F. Berthiaume, Y. Nahmias, ML. Yarmush, Selective targeting of pigmented retinal pigment epithelial (RPE) cells by a single pulsed laser irradiation, Opt Express. 16(14):10518-28 (2008) 11. N. Parashurama, Y. Nahmias, C. H. Cho, D. van Poll, A. W. Tilles, F. Berthiaume, M. L. Yarmush, Activin alters the kinetics of endoderm induction in ES cells cultured on collagen gels, Stem Cells 26(2):474-84 (2008) 12. D. van Poll, B. Parekkadan, C. H. Cho, F. Berthiaume, Y. Nahmias, A. W. Tilles, M. L. Yarmush, Mesenchymal Stem Cell-Derived Molecules Reduce Hepatocellular Death and Enhance Regeneration in a Rat Model of Fulminant Hepatic Failure, Hepatology 47(5):1634-43 (2008) 13. Y. Nahmias, J. Goldwasser, M. Casali, D. van Poll, T. Wakita, R. T. Chung, M. L. Yarmush, ApoB dependent HCV Secretion is Inhibited by the Grapefruit Flavonoid Naringenin, Hepatology 47(5):1437-45 (2008) 14. Y. Nahmias, M. L. Yarmush, R. T. Chung, Reply: A Grapefruit a Day for Patients Infected with Hepatitis C? , Hepatology 47(6):2142-3 (2008) 15. O. Benny, O. Fainaru, A. Adini, F. Cassiola, L. Bazinet, I. Adini, E. Pravda, Y. Nahmias, S. Koirala, G. Corfas, R. J. D'Amato, J. Folkman, An orally delivered small-molecule formulation with antiangiogenic and anticancer activity, Nature Biotechnology. 26(7):799-807 (2008) 16. R. Jindal, Y. Nahmias, A. W. Tilles, F. Berthiaume, M.L. Yarmush. Amino acid mediated heterotypic interaction govern performance of hepatic tissue model. FASEB J. 23(7):2288-98 (2009). 17. T. J. Maguire, E. Novik, P. Chao , J. Barminko, Y. Nahmias, M. L. Yarmush, K. C. Cheng, Design and application of microfluidic systems for in vitro pharmacokinetic evaluation of drug candidates. Curr Drug Metab. 10(10):1192-9. (2009) 18. H. Tolboom, P. Roos, M.L. Izamis, J. Milwid, K. Uygun, Y. Nahmias, F. Berthiaume, M.L. Yarmush, Recovery of warm ischemic livers by ex vivo normothermic perfusion in a rat model of liver transplantation. Transplantation. 87(2):170-7 (2009) 19. S. Kidambi, J. Yarmush, W. Fong, S. Kamath, J. SchianodiCola, Y. Nahmias, Propofol induces a Dose-Dependent Alteration in the Expression of c-Fos and Egr-1 in Neuronal Cells, Neuroreport 6;20(7):657-62 (2009) 20. S. Kidambi, R. Yarmush, E. Novik, P.B. Chao, M.L. Yarmush, Y. Nahmias, Oxygen-mediated enhancement of metabolism, functional polarization, gene expression, and drug clearance in cocultures of primary hepatocytes, Proc Natl Acad Sci USA 106(37):15714-9 (2009) 21. J. Wagoner, A. Negash, O.J. Kane, L.E. Martinez, Y. Nahmias, N. Bourne, D.M. Owen, J. Grove, C. Brimacombe, J.A. McKeating , E. Pécheur , T.N. Graf , N.H. Oberlies , V. Lohmann, F. Cao, J.E. Tavis, and S.J. Polyak Multiple Effects of Silymarin on the Hepatitis C Virus Lifecycle. Hepatology. 51(6):1912-21 (2010). 22. B. Uygun, A. Soto-Gutierrez, H. Yagi, M.L. Izamis, M.A. Guzzardi, C. Shulman, J. Milwid, N. Kobayashi, A. Tilles, F. Berthiaume, M. Hertl, Y. Nahmias, M.L. Yarmush, K. Uygun. Organ

reengineering through development of a transplantable recellularized liver graft using decellularized liver matrix. Nature Medicine. 16(7):814-20. (2010) 23. D. van Poll, Y. Nahmias, A. Soto-Gutierrez, M. Ghasemi, H. Yagi, N. Kobayashi, M. L. Yarmush, M. Hertl, Human Immune-reactivity against Liver Sinusoidal Endothelial Cells from GalTalpha(1,3)GalT-Deficient Pigs, Cell Transplant. 2010;19(6):783-9 24. H. Yagi, A. Soto-Gutierrez, N. Navarro-Alvarez, Y. Nahmias, J. Goldwasser, Y. Kitagawa, A.W. Tilles, R.G. Tompkins, B. Parekkadan, M.L. Yarmush. Reactive bone marrow stromal cells attenuate systemic inflammation via sTNFR1. Mol Ther. 18(10):1857-64. (2010) 25. A. Soto-Gutierrez, N. Navarro-Alvarez, H. Yagi, Y. Nahmias, M. L. Yarmush, N. Kobayashi, Engineering of an Hepatic Organoid to Develop Liver Assist Devices. Cell Transplant. 2010;19(6):815-22 26. J. Goldwasser, P. Y. Cohen, E. Yang, P. Balaguer, M. L. Yarmush, Y. Nahmias, Transcriptional Regulation of Human and Rat Hepatic Lipid Metabolism by the Grapefruit Flavonoid Naringenin: Role of PPARα, PPARγ and LXRα, PLoS One.5(8). pii: e12399 (2010) 27. M. L. Izamis, N. S. Sharma, B. Uygun, R. Bieganski, N. Saeidi, Y. Nahmias, K. Uygun, M.L. Yarmush, F. Berthiaume. In situ metabolic flux analysis to quantify the liver metabolic response to experimental burn injury. Biotechnol Bioeng. 2011 Apr;108(4):839-52. 28. P. Koria, H. Yagi, Y. Kitagawa, Z. Megeed, Y. Nahmias, R. Sheridan, M.L. Yarmush, Selfassembling elastin-like peptides growth factor chimeric nanoparticles for the treatment of chronic wounds. Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1034-9 29. J. Goldwasser, P.Y. Cohen, W. Lin, D. Kitsberg, P. Balaguer, S. J. Polyak, R. T. Chung, M. L. Yarmush, Y. Nahmias, Naringenin Inhibits the Assembly and Long-Term Production of Infectious Hepatitis C Virus Particles Through a PPAR-Mediated Mechanism, J Hepatol. 2011 Feb 24. [Epub ahead of print] 30. M. Shulman, M. Cohen, A. Soto-Gutierrez, H. Yagi, H. Wang, J. Goldwasser, C.W. LeeParsons, O. Benny-Ratsaby, M.L. Yarmush, Y. Nahmias, Enhancement of Naringenin Bioavailability by Complexation with Hydroxypropoyl-β-cyclodextrin, PLoS One. 2011 Apr 6;6(4):e18033. 31. M.L. Izamis, K. Uygun, N. S. Sharma, B. Uygun, R. M. Bieganski, Y. Nahmias, M. L. Yarmush, F. Berthiaume. Development of Metabolic Indicators of Hypermetabolism: VLDL and Acetoacetate Are Highly Correlated to Severity of Burn Injury in Rats, submitted 32. E. Merquiol, D. Uzi, T. Mueller, D. Goldenberg, Y. Nahmias, R. Xavier, B. Tirosh, O. Shibolet, HCV causes chronic endoplasmic reticulum stress leading to adaptation and interference with the unfolded protein response, submitted 33. K. Hirschberg, M. Asher, Y. Nahmias, E.H. Sklan, The hepatitis C virus NS5A is a major lipid droplets binding protein, submitted 34. M.A. Guzzardi, D. Kitsberg, N. Habib, N. Friedman, B. Uygun, K. Uygun, R.T. Chung, Y. Nahmias, Transcriptional-metabolic effect of HCV infection in primary human hepatocytes, in preparation

Book Chapters 1. Y. Nahmias, F. Berthiaume, M. L. Yarmush, Integration of Technologies for Hepatic Tissue Engineering, Adv Biochem Eng Biotechnol. 103:309-29, (2007) 2. Y. Nahmias, M. L. Yarmush, Tissue Engineering Application in General Surgery, Fundamentals of Tissue Engineering and Regenerative Medicine, May (2009) 3. K. R. King, R. Jindal, Y. Nahmias, M. L. Yarmush Dynamic Gene-Expression Analysis in a Microfluidic Living Cell Array (mLCA), Microdevices in Biology and Medicine (Methods in Bioengineering Series), Aug (2009)

4. M. Shulman, Y.Nahmias, Long Term Culture and Co-Culture of Primary Rat and Human Hepatocytes, Epithelial Cell Culture Protocols, in press

Edited Books 1. Microdevices in Biology and Medicine (Methods in Bioengineering Series), Y. Nahmias, SN. Bhatia, Editors, Artech House, Aug (2009)

Conference Presentations and Published Abstracts 1. E. Ezra, E. Keinan, Y. Nahmias, Analysis of Flow Irregularities In Microfluidic Devices, Presented at the Hebrew University Center for Nanoscience and Nanotechnology Annual Conference, Dead Sea, Israel, June, 2011 2. D. Bavli, Y. Nahmias, Development of One-Step Microfluidic Isolation-Patterning of Stem Cell Mixtures, Presented at the Hebrew University Center for Nanoscience and Nanotechnology Annual Conference, Dead Sea, Israel, June, 2011 3. Y. Nahmias, Microliver Technologies: Design and Application of Metabolic Programming. Keynote Presentation at the 6th International Conference on Microtechnologies in Medicine and Biology, Lucerne, Switzerland, May, 2011 4. Y. Nahmias, Establishment of liver-like tissue in vitro through endothleial-mediated hepatocyte recruitment, polarization and function: applications in drug screening and HCV infection. Presented at FISEB/ILANIT 2011, Eilat, February, 2011 5. Y. Nahmias, Oxygen-Mediated Enhancement of Primary Hepatocyte Metabolism, Functional Polarization, and Gene Expression: Achieving In Vivo Levels of Drug Clearance and HCV Infection In Vitro, Predictive Human Toxicity and ADME/Tox Studies, Brussels, Belgium, January, 2011 6. E. Ezra, Y. Nahmias, Development of a High-throughput Transcriptional Activity Array, Presented at the NanoIsrael 2010 Conference, Tel Aviv, Israel, November, 2010 7. Y. Nahmias, Metabolic Programming and the Design of HCV Therapeutics, Presented at the 2nd Molecular Viral Hepatitis Conference, Tel-Aviv Sourasky Medical Center, June 2010 8. E. Ezra, Y. Nahmias, Development of a High-throughput Transcriptional Activity Array, Presented at the Hebrew University Center for Nanoscience and Nanotechnology Annual Conference, Ashkelon, Israel, June, 2010 9. J. Goldwasser, RT. Chung, Y. Nahmias, Naringenin Inhibits the Assembly and Long-Term Production of Infectious Hepatitis C Virus (HCV) Particles Through a PPAR-Mediated Mechanism. Presented at EASL International Liver Congress, Vienna, Austria, April, 2010 10. S. Kidambi, Y. Nahmias, Oxygen-mediated enhancement of primary hepatocyte metabolism, functional polarization, and gene expression: Achieving in vivo levels of drug clearance and HCV infection in vitro. Presented at EASL International Liver Congress, Vienna, Austria, April, 2010 11. J. Goldwasser, RT. Chung, Y. Nahmias, Naringenin inhibits HCV assembly through activation of PPARα and inhibition of LXRα. Presented as the 16th International Symposium on HCV and Related Viruses, Nice, France, October, 2009 12. J. Goldwasser, S. Polyak, RT. Chung, Y. Nahmias, Inhibition of HCV assembly: A new tool in the STAT-C toolbox. Presented as the 16th International Symposium on HCV and Related Viruses, Nice, France, October, 2009 13. Y. Nahmias, J. Goldwasser, RT. Chung, Development of the Grapefruit Flavonoid Naringenin as an HCV Therapy: Efficacy, Mechanism and Delivery. Presented at the Japan Cell Transplantation Society Meeting, Okayama, Japan, April 2009.

14. Y. Nahmias, J. Goldwasser, RT. Chung, Development of the Grapefruit Flavonoid Naringenin as an HCV Therapy: Efficacy, Mechanism and Delivery. Presented at the Japan Cell Transplantation Society Meeting, Okayama, Japan, April 2009. 15. Y. Nahmias, Establishment of Liver-like Tissue In Vitro Through Endothelium-Mediated Hepatocyte Recruitment, Polarization and Function. Presented at the Japan Cell Transplantation Society Meeting, Okayama, Japan, April 2009. 16. Y. Nahmias, J. Goldwasser, M. Casali, RT. Chung, ML. Yarmush, Modulating the Lipoprotein-Dependent HCV Secretion, Presented at the AICHE Meeting, Philadelphia, PA, November 2008 17. S. Kidambi, J.Yarmush, W. Fong, S. Kamath, J. Schianodicola, Y. Nahmias, Propofol Induces a GABA-Independent Expression of C-Fos and Egr-1 In Neuronal Cells, Presented at the AICHE Meeting, Philadelphia, PA, November 2008. 18. J. Goldwasser, RT. Chung, Y. Nahmias, Long term ApoB-dependent HCV secretion is blocked by the grapefruit flavonoid naringenin, Presented as the AASLD Annual Meeting, San Francisco, CA, October 2008 19. J. Goldwasser, RT. Chung, Y. Nahmias, Long term ApoB-dependent HCV secretion is blocked by the grapefruit flavonoid naringenin, Presented as the 15th International Symposium on HCV and Related Viruses, San Antonio, TX, October 2008 20. Q. Song, R. R. Delgado, M. Latina, F. Berthiaume, Y. Nahmias, M. L. Yarmush, In Vitro Studies of Selective Targeting of Pigmented Retinal Pigment Epithelial (RPE) Cells and Subsequent Bio-Stimulation, Presented at the AICHE Meeting, Salt Lake City, UT, November 2007. 21. R. Jindal, K. R. King, Y. Nahmias, M. Toner, M. L. Yarmush, Microfluidic Co-Culture Arrays For Parallel Screening Of Pair-Wise Cell-Cell Communication Between Multiple Cell Types, Presented at the AICHE Meeting, Salt Lake City, UT, November 2007. 22. R. Jindal, Y. Nahmias, A. W. Tilles, F. Berthiaume, M. L. Yarmush, Endothelial Cells Induced Early Recovery Of Hepatocytes In An Organotypical Model, Presented at the AICHE Meeting, Salt Lake City, UT, November 2007. 23. N. Parashurama, Y. Nahmias, C. H. Cho, D. van Poll, A. W. Tilles, F. Berthiaume, M. L. Yarmush, Activin Alters The Kinetics Of Endoderm Induction In Embryonic Stem Cells Cultured On Collagen Gels. Presented at the AICHE Meeting, Salt Lake City, UT, November 2007. 24. Y. Nahmias, M. Casali, N. Parashurama, R. Chung, M. L. Yarmush, “Hepatitis C Virus Lifecycle along the Lipoprotein Pathway.” Biomedical Science and Engineering Seminar Series, Massachusetts General Hospital, December, 2006. 25. Y. Nahmias, M. Casali, L. Barbe, F. Berthiaume, M. L. Yarmush, Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary hepatocytes, Proceedings of the 2006 Annual Fall Meeting, Presented at the Biomedical Engineering Society Meeting, Chicago, IL, October, 2006. 26. Y. Nahmias, I. Kramvis, L. Barbe, M. Casali, F. Berthiaume, M. L. Yarmush, A novel formulation of oxygen carrying-matrix enhances liver specific function of cultured hepatocytes, Proceedings of the 2006 Annual Fall Meeting, Presented at the Biomedical Engineering Society Meeting, Chicago, IL, October, 2006. 27. Y. Nahmias, M. Casali, L. Barbe, F. Berthiaume, M. L. Yarmush, Liver endothelial cells promote LDL-R expression and the uptake of HCV-like particles in primary hepatocytes, Presented at the AICHE Meeting, San Francisco, CA, November 2006. 28. Y. Nahmias, I. Kramvis, L. Barbe, M. Casali, F. Berthiaume, M. L. Yarmush, A novel formulation of oxygen carrying-matrix enhances liver specific function of cultured hepatocytes, Presented at the AICHE Meeting, San Francisco, CA, November 2006.

29. L. Barbe, H. Tolboom, Y. Nahmias, F. Berthiaume, M. L. Yarmush (2005) “In-Vitro Model of Hepatic Ischemia/Reperfusion Injury” Presented at the AICHE Meeting, Cincinnati, OH, October 2005. 30. Y. Nahmias, M. Casali, F. Berthiaume, M. L. Yarmush, Sinusoidal endothelial regulation of LDL-uptake in hepatocyte cultures, Proceedings of the 2005 Annual Fall Meeting, Presented at the Biomedical Engineering Society Meeting, Baltimore, MD, September, 2005. 31. Y. Nahmias and D. J. Odde, Direct Writing And Hepatocyte Co-Culture Support Of Endothelial Tube-Like Network Formation In-Vitro. Proceedings of the 2003 Annual Fall Meeting, 2.1.5. Presented at the Biomedical Engineering Society Meeting, Nashville, TN, October, 2003. 32. Y. Nahmias and D. J. Odde, Generalized Analysis And Design Of Radiation Force-Based Systems (Optical Trapping, Laser Guidance).Proceedings of the 2003 Annual Fall Meeting, 5.6.4. Presented at the Biomedical Engineering Society Meeting, Nashville, TN, October, 2003. 33. Y. Nahmias and D. J. Odde, Direct Capillary Formation in Liver Organoid Cultures." Presented at the Medical Alley Conference, St Paul, MN, November, 2003. 34. Y. Nahmias and D. J. Odde, Laser-Guided Direct Writing for Three Dimensional Tissue Reconstruction. Presented at the Design of Medical Devices Conference, Minneapolis, MN, April, 2003. 35. Y. Nahmias and D. J. Odde, Laser-Guided Direct Writing for Tissue Engineering Applications.Presented at the Medical Alley Conference, Minneapolis, MN, November, 2002. 36. Y. Nahmias and D. J. Odde, A dimensionless parameter for escape force calculation and general design of radiation force-based systems such as laser trapping and laser guidance. Biophysical Journal, 82, 166a. Presented at the Biophysical Society Annual Meeting, San Francisco, CA, February, 2002. 37. Y. Nahmias and D. J. Odde, Laser-guided direct writing of multi-potent adult progenitor cells for tissue engineering. Annals of Biomedical Engineering, 29(1), S-157. Presented at the Biomedical Engineering Society Meeting, Durham, NC, October, 2001. 38. Y. Nahmias and D. J. Odde, Multi-potent adult stem cell patterning by laser-guided direct writing. Presented at the Fifth Annual Hilton Head Workshop on Engineering Tissues, Hilton Head, SC, February, 2001. 39. Y. Nahmias and D. J. Odde, Multi-potent adult stem cell patterning by laser-guided direct writing.Presented at the Design of Medical Devices Conference, Minneapolis, MN, April, 2001. One of 12 Presidential Sesquicentennial Conferences

Granted Patents 1. E. Shimoni, O. Ramon, I. Kopelman, S. Mizrahi, N. Salzman, Y. Nahmias, A. Oren. Oral Delivery of Proteins and Peptides, International Patent (PCT/IL2008/000539), April 27, 2008. 2. Y. Nahmias, RT. Chung, ML. Yarmush, Naringenin Complexes and Methods of Use Thereof, International Patent (PCT/US2009/059864), October 7, 2009. 3. Y. Nahmias, E. Novik, ML. Yarmush, R. Freedman, Compositions and Methods of Functionally Enhanced In Vitro Cell Culture System, International Patent (PCT/US2009/065781), November 24, 2009. 4. Y. Nahmias, Nuclear Receptor Screening Array, Provisional Patent Application, July 28, 2011 5. Y. Nahmias, Method to induce specific metabolic programs by pharmaceutically modulating the underlying transcription factor network, July 28, 2011

Invited Seminars 1. MicroLiver Technologies, Department of Chemical Engineering, Northeastern University, August, 2011 2. Metabolic Reprogramming, Institute of Virology, Universitätsklinik Essen, Germany, January 2011 3. Development of a High-Throughput Transcriptional Activity Array, Department of Genetics, Hebrew University, October, 2010 4. Metabolic Reprogramming, Department of Biochemistry, Hadassah Medical School, October, 2010 5. Metabolic Reprogramming, Institute of Biochemistry, Food Science and Nutrition, Faculty of Agriculture, Hebrew University, October, 2010 6. Development of a High-Throughput Transcriptional Activity Array, Katholieke Universiteit Leuven (SCIL), August, 2010 7. Metabolic Reprogramming, Interdepartemental Stem Cell Institute Leuven (SCIL), August, 2010. 8. Metabolic Reprogramming, The 73rd Meeting of the Board of Governors of the Hebrew University of Jerusalem, June, 2010. 9. Liver Tissue Engineering and the Rational Design of HCV Therapeutics, Liver Unit, Hadassah University Hospital, February, 2010. 10. Liver Tissue Engineering and the Rational Design of HCV Therapeutics, Center for Innovative Regenerative Therapies, Children’s Hospital, University of Pittsburgh, December, 2009. 11. Liver Tissue Engineering, Biopolymers and Stem Cell-Based Tissue Engineering Workshop, School of Pharmacy, Hadassah University Hospital, December, 2009. 12. Host Lipid Metabolism as the target of new HCV antivirals, Computational Biology Seminar, The Hebrew University of Jerusalem, School of Engineering, November, 2009. 13. Rational design of HCV therapeutic, Research and Innovation in Medical Technologies Workshop, Hadassah University Hospital, November, 2009. 14. Host Lipid Metabolism as the target of new HCV antivirals, Gastrointestinal Unit Seminar, Tel Aviv Sourasky Medical Center, April, 2009. 15. Host Lipid Metabolism as the Target of Flavonoid-derived HCV Antivirals, Liver Center Seminar Series, Albert Einstein College of Medicine, January, 2009. 16. Microfabrication and the challenge of rationale design of HCV therapeutics, Developmental and Cell Biology Seminar Series, Hebrew University of Jerusalem, December, 2008. 17. Modulating the Lipoprotein-Dependent Hepatitis C Virus Uptake and Release, Gastrointestinal Unit Seminar Series, Massachusetts General Hospital, July, 2008. 18. Liver Tissue Engineering, Cornell, April, 2008 19. Integration of Technologies for Hepatic Tissue Engineering, Tel Aviv University, March, 2008 20. Liver Tissue Engineering: From Lasers to Grapefruits, Bioengineering Seminar Series, University of Pennsylvania, March, 2008 21. Methods & Applications of Cell Patterning, Short Course: An Introduction to Microtechnology and Microfluidics for Biology and Medicine, 2nd Annual Methods in Bioengineering Conference, July, 2007. 22. Hepatitis C Virus Lifecycle along the Lipoprotein Pathway, Biomedical Science and Engineering Seminar Series, Massachusetts General Hospital, December, 2006. 23. Endothelium-Mediated Hepatocyte Recruitment and Receptor Expression. Department of Biomedical Engineering, Technion, Israel-Institute of Technology, July, 2005.

24. Direct Writing Techniques for Three-Dimensional Tissue Engineering. Presented at Complex Fluids / Microstructure / Macromolecules Seminar, Technion, Israel-Institute of Technology, September, 2003.

Professional Membership Panel Member for the ERC Starting Grant 2011 (LS9) Biomedical Engineering Society (BMES) American Institute of Chemical Engineers (AICHE) European Association for the Study of the Liver (EASL)

Journal Peer Review PNAS, Tissue Engineering, Applied Optics, PLoS One, BioTechniques, Cellular and Molecular Bioengineering, Biotechnology and Bioengineering, Biophysics, Cytotechnology, Annals of Biomedical Engineering

Research Support Close to $4.3 million in research funding in Israel, and $1 million at Harvard ACCEPTED: 1707/11 (PI: Yaakov Nahmias) 01/08/2011 Israel Science Foundation (ISF) – Equipment Grant $150,000 “Fluorescent Activated Cell Sorter System” This is a one-time grant to purchase an institutional FACS machine. ONGOING: 0398307 (PI: Yaakov Nahmias) 01/12/2010-30/11/2013 Japan – Israel Collaboration, Ministry of Science $300,000 “Organ Engineering: Development of Artificial Liver Tissue Using Embryonic Stem Cells ” This three-year collaboration with Prof. Yuko Kitagawa and Dr. Hiroshi Yagi at Keio University, aims to develop a transplantable liver organoid using advanced decellularization technology and human embryonic stem cells. AOR 266777 (PI: Catherine Verfaillie) 01/01/2011-00/00/2016 FP7-HEALTH-2010-Alternative-Testing $10,000,000 “HeMiBio – Hepatic Microfluidic Bioreactor ” The goal of HeMiBio is to develop a hepatic microfluidic bioreactor from human iPSC-derived hepatocytes, hepatic sinusoidal endothelial cells (HSEC) and stellate cells (HSC), suitable for inclusion in a repeated dose toxicity testing strategy of pharmaceuticals/cosmetic ingredients. (PI: Yaakov Nahmias) 01/03/2011-28/02/2014 Marie Curie International Reintegration Grants (IRG) $148,190 “Gene network-based maturation of embryonic stem cell-derived hepatocytes in a microfabricated array”

The goal of this project is to develop a microfabricated transcription factor activity array for the study of the functional maturation of embryonic stem cell-derived hepatocytes. (PI: Shashi Murthy) 04/01/2010-03/31/2013 NIH/NBIB $317,771 “Microfluidic Cell Separation for Tissue Engineering and Regenerative Medicine” This subcontract project aims to develop a microfluidic isolation and culture of skin stem cells from discarded tissue for applications in regenerative medicine and burn injuries. 242699 (PI: Yaakov Nahmias) 04/01/2010-03/31/2015 European Research Council – Starting Grant (FP7) $2,970,252 “Microfabrication-Based Rational Design of Transcriptional-Metabolic Intervention for the Treatment of Hepatitis C Virus (HCV) Infection” This project aims to develop a high-throughput screening platform for the development of combinatorial transcriptional-targeted drugs for the treatment of HCV and other metabolic diseases. 1K01-DK080241 (PI: Yaakov Nahmias) 03/01/2008-01/31/2013 NIH/NIDDK $702,000 “Gene network-based differentiation of stem cells in a microfabricated array” The goal of this project is to develop a microfabricated transcription factor activity array for the control of stem cells differentiation using real-time functional genomics. P41 EB002503 (PI: Mehmet Toner) 04/01/2009-03/31/2014 NIH/NBIB $1,198,514 “Bio-MicroElectroMechanical Systems (BioMEMS) Resource Center” The BioMEMS resource center is aimed to provide unique capabilities to biomedical investigators in order to efficiently use microsystems technologies to probe, perturb, engineer, sort, and analyze living cells and tissues. COMPLETED: P30-DK040561 (PI: Yaakov Nahmias) 04/01/2009- 03/31/2011 Harvard Clinical Nutrition Research Center $20,000 “Study of the Hepatitis C Virus (HCV) antiviral properties of polymethoxyflavones” This proposal aims to study the ability of polymethoxyflavones to block the replication and assembly of HCV as a potential dietary supplement-derived therapy P30-DK040561 (PI: Yaakov Nahmias) 06/01/2008- 05/31/2010 Harvard Clinical Nutrition Research Center $18,000 “Grapefruit flavonoid naringenin for the treatment of HCV infection” The goal of this project is to elucidate the pharmacokinetic profile of naringenin in HCV infected patients and its ability to lower viral titer. 8512-856 (PI: Yaakov Nahmias) 01/01/2008-12/31/2009 Shriners Burns Institute $60,000 “Self-assembled KGF Nanoparticles for Enhanced Epidermal Regeneration” This project aim to develop a growth factor production and delivery platform that will accelerate wound healing and regeneration in burn injury.

8505-856 (PI: Yaakov Nahmias) 07/01/2007-07/01/2009 Shriners Burns Institute $122,362 “Novel Oxygen Carrying Dermal Substitute & Topical Oxygen Treatment” The main objective of this fellowship is to develop an oxygen carrying dermal substitute based on novel fluorocarbon – collagen complex for the treatment of burn injuries and chronic wounds.