Nanostructured PCL-Magnetite Materials, Physicochemical Properties and Biocompatibility for Biomedical Applications C. Chapa1, K. Y. Castrejón1, C. A. Martínez1, T. Pasquoto2, R. de Lima2, L. Fernandes3, P. E. García1
1
2
Institute of Engineering and Technology, University of Juarez, Henry Dunant #4600, Ciudad Juárez, ZIP 32310 México
Department of Biotechnology, University of Sorocaba. Rod. Raposo Tavares, Km 92,5 - Artura, Sorocaba São Paulo, 18023-000, Brazil 3
Department of Environmental Engineering, State University of Sao Paulo. Avenida Três de Março, #511, Alto da Boa Vista, Sorocaba - SP, 18087-180, Brazil E-mail:
[email protected]
Abstract The development of biomedical applications such as hyperthermia and controlled drug delivery has been possible due to the properties of nanostructured materials. Magnetic nanoparticles (MNP) have been used in this application due their properties associated with the dissipation of thermal energy in presence of external stimulus. On the other hand, biocompatible polymeric materials, such as PCL, have been developed extensively for controlled release due to its biocompatibility and biodegradability. PCL_MNP nanostructure using emulsion/evaporation method was obtained. The influence of two surface modified magnetite nanoparticles in physicochemical and biocompatibility properties was evaluated in order to assess their suitability as Nanomedicine system. The PCL_MNP nanostructure has an average particle size of 454 ± 6 nm; assessment of stability was carried out by measuring hydrodynamic diameter, zeta potential, polydispersity index conducted over a period of 120 days, the nanostructured material were found to be stable in solution. To achieve the nanocomposite, magnetite nanoparticles (M) with average size 13.45 nm were obtained by chemical coprecipitation and their surface was modified by silanol condensation (MS) and chemisorption of oleic acid (MOA and MSOA) with the development of ferrofluids. It was deduced from TGA results that the difference between weight loss of ferrofluids and nanoparticles was the amount of coating, 30 % for MOA and 46 % for MSOA. Besides, it was shown by FTIR that oleic acid forms a coating by chemisorption on both magnetite (M) and magnetite-silica (MS) nanoparticles; the band 2855 cm-1 attributed to the free OH in oleic acid is decreased in MOA and MSOA. Then PCL_MNP composite were obtained by emulsion/evaporation method; assessment of stability was carried out by measuring hydrodynamic diameter, zeta potential, polydispersity index conducted over a period of 120 days, the nanostructured material were found to be stable in solution. Furthermore, the MTT-based cell viability assay showed a dependence of the materials concentration in cell survivorship; in PCL_MOA cell viability was found between 44.5 to 84.6 % and PCL_MSOA was 48 to 82.6 % varying concentration from 6.0 to 0.03 % in volume, respectively. Keywords coating materials, nanostructured materials, chemical synthesis References [1] C. Berry, Curtis S. J Phys D: Appl Phys 36R (2003) pp 182-197. [2] R Grillo, A. Pereira, N. de Melo, R. Porto, L. Feitosa, P. Tonello, N. Dias, A. Rosa, R. de Lima, L. Fernandes. J. Hazard. Mater. 186 (2011) pp 1645–1651. [3] H. Misara, H. Fessi. J Colloid and Interface Sc 300 (2006) pp 584–90. [4] C. Chapa, J. Roacho. C. Martínez, I. Olivas, F. Jimenez, K. Castrejon, P. Garcia J. Alloys Comp. (2014), http://dx.doi.org/10.1016/j.jallcom.2014.01.155. [5] R. Payman, A. Holmgren. App Surface Sc 255 (2009) pp 5891–5895 [6] P. Tartaj, T. González, M. Bomati, C. Serna, P. Bonville. Phys Rev B, 69:9 (2004), pp 4401-4408. [7] R. Terrazas, C. Martínez, C. Rodríguez, H. Monreal, P. García. Cent. Eur. J. Chem. 8 no. 5 (2010) pp 10411046.
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