NBC No Cutoff NBC Cutoff NBC + MEGAN Cutoff - PLOS

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20. 40. 60. 80. 100. 50. 100. 200. 400. 0. 20. 40. 60. 80. 100. NBC Cutoff. 50. 100. 200. 400. 0. 20. 40. 60. 80. 100. NBC + MEGAN Cutoff. 50. 100. 200. 400. 0. 20.

Recovery (%)

NBC No Cutoff

100

80

80

80

60

60

60

40

40

40

20

20

20

0

0

0

100

200

400

50

100

200

400

100

100

100

80

80

80

60

60

60

40

40

40

20

20

20

0

0

0

50

100

200

400

100 Coverage (%)

NBC + MEGAN Cutoff

100

50 Erroneous recovery (%)

NBC Cutoff

100

80 60 40 20 0

Kingdom Phylum Class Order Family Genus 50 100 200 400 Simulated read length (bp)

50

100

200

400

100

100

80

80

60

60

40

40

20

20

0

0 50 100 200 400 Simulated read length (bp)

50

100

200

400

50

100

200

400

50 100 200 400 Simulated read length (bp)