New insights into morphological, stereological and ...

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118 interdisciplinary. New insights into morphological, stereological and functional studies of the adrenal gland under exposure to the potent goitrogen thiourea.
Interdiscip Toxicol. 2018; Vol. 11(1): 101–107. doi: 10.2478/intox-2018-00xx

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interdisciplinary

Copyright © 2018 SETOX & IEPT CEM SASc. This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (https://creativecommons.org/licenses/ by-nc-nd/4.0).

ORIGINAL article

New insights into morphological, stereological and functional studies of the adrenal gland under exposure to the potent goitrogen thiourea Arijit Chakraborty, Deotima Sarkar, Priyanki Dey, Amar K. Chandra Endocrinology and Reproductive Physiology Laboratory, Department of Physiology, University of Calcutta, 92, APC Road, Kolkata-700 009, India ITX110118A05  •  Received: 29 November 2017  •  Accepted: 01 February 2018

ABSTRACT Thiourea (thiophen-3-yl-acetic acid) is a well established antithyroid drug used for treating hyperactivity of the thyroid gland as it blocks the conversion of thyroxine (T4) to triiodothyronine (T3) in peripheral tissues. Human exposures to thiourea include contaminated drinking water and vegetables for its extensive use in fertilizers. Chronic thiourea exposure can cause thyroid dysfunction leading to redox imbalance. However, such effects on morphological, quantitative, functional and hypothalamo-pituitary-adrenocortical axis (HPA) analysis of the adrenal gland are yet to be explored. The aim was to explore the effect of thiourea on structural and functional status of the adrenocortical region with special reference to the HPA axis. Control rats were fed a normal laboratory standardized diet whereas to experimental rats, thiourea at a dose of 0.3 mg/day/Kg body weight was administered orally, once every day for consecutive 28 days. Histology and histometry, including morphometry of the adrenal, adrenal ∆5 3β HSD and 17β HSD activity, LPO level and serum corticosterone profile were assessed. Statistical significance was studied by ‘Mann-Whitney U’ test at p