Nucleic Acids Research. 3360. 3480. 3600. 3720. 3840. 3980. 4080. 4200. 4320. 4440. 4560. 4680. 4800. 4920. 5040. $160. 5280. AGAGMA. $400.
Nucleic Acids Research
Volume 17 Number 13 1989
Nucleotide sequence of cDNA encoding
P4N protein in the mouse cerebellum
Teiichi Furuichil, Shingo Yoshikawa' 2 and Katsuhiko Mikoshibal2 'Division of Behavior and Neurobiology, National Institute for Basic Biology, 38 Nishigonaka, Myodaijicho, Okazaki 444 and 21nstitute for Protein Research, Osaka University, 3-2 Yamadaoka, Suita 565, Japan Submitted June 13, 1989 EMBL accession no. X15373
P400
protein is a membrane-associated glycoprotein that is phosphorylated by protein kinase A (1, 2). P400 cDNA clones were isolated from a mouse cerebellum cDNA library constructed in lambda gtll (3). The library was immunoscreened with three independent monoclonal antibodies against P400 (1,2). The sequence of 9,871 bp contains a single large open reading frame beginning with ATG at nucleotide 329 and ending with TAG at nucleotide 8,576, and encodes a 2,749 amino-acid protein (3). Most of the sequence was determined from at least two independent clones (3). Sequence variants which might be due either to cloning artefacts or to something else are as follows: T to C, T to C and C to T at nucleotide position 763, 826 and 1024, respectively. In all cases no amino-acid change occurs. According to our preliminary data, the transcription initiation site is 23 nucleotides further upstream than the 5' end shown
here.
GCTGUGCtTTCCTCAAGCCTGCCGGGtGGGAGGWACrGWGTGTGr.TGTGGAGGA(GTrGGAdCACGAGGGTGG"AGAA^AGCGCACGCGGAGGAG4TG;TGGTGT CCGTCCCAtCCTAAC;WAkACT=tCTCCTCCCCuATGMTGC6rrkCG=&AltGrCA=TCCTrrCGGTCtACCrGAAAGGCTGCTCCCGGTCACCAGGAGC TG^rATTACA"ACCAGGAtTTGCACCTt=TGtCCrATTG$GCTCMTtGACCTGGGTCAGGCTTTMVACGrGACAtGTCTCGAAATGTrATtTCCTACATAt T.CACATfTGTTCTCTGtATG=GATCTACCAAtGG^rATTTrATCiC TTAGCCTGGTTGAtGACCGTTCr.T6TGTACAAGAGCCV,60=TAACiATCCACCAA
GAAATTCAAMTOCrcTCtTTAAGCTA6CTATGAAtCTGATAC ACAAAC'TTrCTGGuAAACT(CT AGCATACAGATGCAGTGCTGCTCAACAA ATrCTscAtrCTGCAGoAC ATsacro AATGTGGGACCTCATCCAATATGGCAACGTGAtCCAGC c cTGCCATT TC&AGCAATAAATi CCTCATrCTCATAACACTCCAGCCTtGCTACrGA:&TATG;CCCGCCTACGTTGC"=TGAAATCAAGGGTCCGGTTTTACATTCAACCATTTTACAAGCTTCGCTC CATCCCAGATrCGTCCr
TAG:ACTrTArCTGTTTTAATCCTrTCCCATCCCCrCGCCAGCTCTACAtACAGTTCAOCTCtGGATAACCCiGCTGCAATCAGGTCAACTC
TTCTAAAGGAcrArcTcGATGTG;TAcTCTrCCAtGcscA^GCAGAAGTT TCT T TC6GAtcrcscTGGM=GTGGGAAGTGAG(TcAGTCAcACGACCc ATCTWCCTGCtATGCCtACTOWA6CCrTCCCTTCGCgTCACCATCTTACAl;GCUTTrGCGACTtTAGeAGTACCGA,TGCTTTCAGAGAATGOCCTGAGrTTTCAGCC CTCAGTrCC*TC.TCAG6TOCATCTCArClACGTTAG AAAAAATGGTTACTCCTTGGTTCCTGCC6TaCTCrTCTCCTCATCTTTGAGCTAGA CrcTCAACTctAATrAcStGGArAcAtGCMTCATcATGATcMTAAcT
CAOCTrG^TC
AGcnATTCTcTQCC_TAGCXcc6W
cc#TcdS
CCCCACrGACtCTCTCGATWCCCrTTCaCACtACTeCCTATT=CTCACACCTCrTGACcAcA,tGACACA^A W=TG6CCTGTrCTCA A 1TTCTACCT6C=
120 240 360 480 600
7 20 840 960 1080 1200 1 320 1440
AAACAT6ACGACAAGGAAGAGGi
15S60
AACATTGC CATACTTTr.TCCCtrGTTCTGUrCTGGCTTCrGG^CTGGCAATTGMTATGAGCAi CTCACACCTGAG AAGTCTGTCCACrTGACWTTGAC TT(GGtTTACrTTTGTACGGGTGGAACTAACTCTGG
1680 1 800 19 20 2040 2160 2280 2400 2520 2640 2760 2880 3000 3 120 3 240
CAr
C
CGTGCTCNdTCrCCAT=CT6V.W 6TtCI ccAWcAcGrdTTGWTT6TCTrTCTAC;occA i
GATcicATAtAcGAACAGAAtATTCTAAc-ATCTcTrCAGCGTTCWksc=TTCicG TGCGG6 G;GATMC60CTGCTTCCGCTOGCACCCtGGCCTCAG6CC.ATGCT6CMCWACATjATTTGCCrGACTCr.CTACAGGTCCTGCGACACTC4CAGCAAGACTACAGGAAGAArcCi GG"TACATOCAMTTGGCTTCAtTAGACCA6TMTAT&TG$CATC CCCTT ATTGTCAC.TACACCGTACTCCTCC AGAAGCACATCAC A |-CA-A'tCATTCTtGGATTACCTCTCTGACCTCtGCGTATCCAtGAACAAGTCiATrCTGTGACACAGGAGCt
TCCWAGcATGCACtTTGTcAc=tGG^
CATCTGTAASGCT6T6CTCAATCCCTGCTCuATCCTGATTGJAGCTGGtrrTCTCTrTrCrTGUCGtTTGMTTC
TTCACTGGGAGMATGCTCTGCAWGCGGGi
GGATC4CAQPkQGTGTGGTCTTCT /-CCCATA-AArTAT=TAYCGTw.ATGTCMArGTT 6T.TAAAiGGGAcuAMGG.CGGGACACCTCAG CTACTrACAGCTTCAGCTGCTCTTTGACcATr.oCTCACD6TACCTGGCACAATCAiATGCTC"CGCAGCTGGATGT TGATCTCATtCTCCGCTGCiTGTCTGACGi GAACCTCCCCT CTCAOCACTTTTrTTATCCTTCA>TcATGr.AccGAr.ATCc6cAGACSrcArTACCTCGtGATATGC6CGACCTGTGGCAGAATTCC cTCTGACAT6GATTr.ATCTACTGTCCAMTOCACTMAAArCATAZ TTAAGGiGACGTTTGCAcWAGATCr TTTGrTrGGAGGAGTACCTiAGATACGTGrTTTTCAuG ArTT=sTTcTCTr.ATArWAAAS;TcAcrTfcACGr.TTt cSTcTAGOCA6TCTCATiTACTTTGGTtTCTACAACTt TTCTGACCTtTc=TTcAAAiCCAAGATcct CrTcScAATTTA.TGT6CAcTGTGA6TATCTAcAT6=TrT scrrGrcrAGAAGTAAAGGrCAGTAcTGAtGGGCTAT6CAcTcGGCGT6AGCTGAt GACCCAGGTGGTGC TXCTTCrG=ATCCT=ACTGCTnCGGCCCCCTGAAGG^AtGTGAAGcAG6cAG AGGAAAGAGGACATCATGGTCATGGACACCAA
5385
Nucleic Acids Research GT TGAAGATCA T TGAAATAC TCCAG TT TAT T T TGAATGTGAGAT TGGAT TATAGGATCTCCTGCCTCC TGTGTATATTTAAGCGAGAGT T TGATGAAAGCAAT TCCC AG TCATCAGAAAC
ATOCTCCGGiWAGCAGC6AGGAAGGGC6AAGTA.ATGT6CCAG(;TGCT6TTGACTTTGiACACAT TGAiGAACAAGCG6AAGGCATCTt TGGAGGAAGtGArGAGA.ACiCACCT T TGGi CCTGGATGA&ATGGT(;GCiGAACC T TCC fCAGGGTCCT& TOCAC T TGiCAATGCATGiC TACCCACC6CTGGTGTCT&=CC TGCiGC TCC TC T T iCGGCAC T TC iGCC AGAGGC GGAGGTCCT iCA=TTCWZAGGT TCiAC TGC TGGT tACTAGCCWATGTGGACAiCTACAAACI,.,ATCAAGCAA6AC T TGGACCiAC T AAGGTCW TGTGGAGiAG TC TG AGC CTGGGTGTA6&U=AAdGTCCCGATGiGCC TATGGA&GAGCCTCC4GTGAAAATGiGCATAAGWACCGAGGAG-."CGAGCAi=CAC TGAA6CACGAGAG6CCAGC AGC T i CAACTACCGiGTGGTGA04AGATTT TGAt TCGACT TAC.6AAGCTC TGC6TrcAGGAGA6=GTCGGT6AGGAAGWX.6GGAAGCAGCiGCAACGAC Tk TGAGGAACi TGGGCGCACi CGCTGTGGT&TGGAGCT(;6TGCAGATCC6CTACGAGAA6MCGAAGACiCAAAGATGCWAGATCAT6CGGC TGGCT6ATGAATTT T tGCAGAAT T T6 TGTrCAGGCiACCAGCAGAi
TCAAGCTTTdCTGCATAAAdACATAAACCtGTTTCTCAAdCCAGGGATC6TGGAGGCAGtGACGATGCA6CACATCTTCiTGAACAACTtCCAGCTGTaAGTGAGATCiACGAGAGAGt GGTCCAGCAdTTTGTTCACtGCATAGAGAtUACGGTCGiAACGTOCAGiATATCAAGTtTCTCCAGAC6ATTGTCAAr,6cAGAAGGGAiATTCATTAAiAAGTGCCAA6ACATGGTCAt GGCTGAGCT fGTCAACTCT6GGAGAGGACGtCC TCGTGTTdTACAATGACkA=TCT TtCCAGACTCT6ATrCAGATGiTGCGGTCCGiGCGTGACCG.ATGGATGAGiACAGCCC TC t CATGTACCA6ATCCATCTGdTGGAGCTCT tGGCCGTGTG6ACAGAGGGCWAATGTGTiCACGGAGAT6AAGTGCAACtOC T TGCTCC6GC TMATGAWCO TCGTd TGGTCAC TCi TGAAWTG6ATCCCCGAGdTTAAGATCG6TTACATTAA.TTCCTGAAT6ACTGCTATGtGGATACGGA6GTGGAGATGiAGGAGAT TTiCACAAGC&JZCACATG TGGiAGT TGT TTGi GAATTTCCT6GTGGACATCtGCAO= T6TAAC AAGCGACAGGAAGCACGCAGACTOCATTCTGGAGAAGTACGTCACTGAAATCGTGATGAGCATCGTCACCACCTTCTTCAG ACTCTGCAGAC=6CAGOCTGTCTTTGTGCAACTCCTGCAAGGCGTGTTCCGAGTTTACCACTGCAACTGGCTGATGCCGAGCCAAAAAGrCTC CTCT=TTdT
GGTWJZAI;dTGCATCCGr,e,TGCTCTCTG;MTAGCCAk.WX=,GGMiTAGCCATTCZTGTTGACCTkACAGCCWTCWAACCtCT TCCTGAA6Tcr'CACAACiT TGTGCAGAi AACAG=T6AXTGG=fTATCAGCCC6AMCGCCGCtCGCAGAGACtCTGTACTGG6AGCATCCAGk.CTACCGWTATCATTGiGAGGTTACA6UCATCGTGtCTGCCCTAGi TC4TGCAGGCTGiGCTGTCTGT6CTCGTGGAT6TTCTACACA4=AGAACT&TCTTCCCC6AGAACAC(;GiTGCCAGGAGdAAATGTGAGir.TGGArG T T t GGACC=Td CATCTGCAA6CTAATAAAA6ATACCAAGCiACTGCTOGA6GAGAATGWAGAAACTATdCATTAAAGTdTTACAGACC6TCAG(;GAAAtGATGACCAAk.ACAGAGGCtATGGAGAG"
5160 5280 5400
GCCTGGTGAGAGGAGGGGCTCCTGGATCTAGTTCCACAAGCAGGGGTGAGATGAG;CCTGGCTGAGGT
TCAGTGTCACCTCCACAAGGAGGGGGCCTCCAACC TGGTCATCGATCTCAT
CAGGGATGOCCCTCTCCCoGCCAAAGCCCAiAGGACiACAGATAiCAGAAGAGr.tCCC(;GATCAGCTCCTGGAAGCATCTGCTGCCACCAGG;AAAGCT TTACCACCTTCCGGAG rGGAGGCICC£CTGATGACCATTACCAGTCTGGGG;AGGGCACCCAGGCTiCAACCACAiA=CAAGGAtGACCTAGAGiTGAGCGCTGTCATCACCATCATGCAGCCTiTCCTGCrCT T
TTGGTGTGTGiGACACTGCAGTT TC TGGAC tGTATT TGTCGG GAGCACAACCGGAGGCTT6GTCTTCTTGGACTGTACATATGAAAGAATGTAGCACTTATCAAC;PTGGAG;CTCTGACGGAGTACTGTCAkGGCCT TGCCtATGAGAACCi
CC TGCAGCTGCTGTGTGACCCACAACCGAGATCTGCAC,AATTTCCTTCGTTGCCAAATAATAAGACCZACTACAAT
GAACTGCATCGCACCCACAGCTCCAATGGCATCGATATCATCACAC-CCCTCATCCTCAiTGATATCAACCCTCTGGGuACAAGCGGAtGrGACC TGGT TTAGAACTGAAGAACAA TG6 TTCGAAGCT6CTACTGGCCATCATGG^AAAAGACACCGAtGTGAAATGCAGAAG.GAfCCTGT^CACAA6TGAGGCCCAACGA^GCTGGT GGAAGTGATCAAGAAGGCCTfACATGCAAGG TGAAGTGCATTTGA6GrATGCGAGAACGGTGAGGATGGiGCTGCCTCACCCAGACr.tGGCCCACAAe,ATCTACATCeTCGCTCACCiGTTGGCTCGGCATAACAAAGAAC TTCAAAe CATGCTGAAiCCTGGAGCCCAGGTGGATGGGGATGAAGCtCTGGAGTTCTACCCGAAGCCACACACACAiAT TGAGT TGTCAGACTGGiCCGGACAATGGAACAGATCGTCT TCCCTGT GCCCAGCATC TGTGAATTOCTGACTACAGGATCGACT tCGAATATAT tACACCACACGCCGCATCGCA&AGGTAGCiAATCAATGACT TCTTCC TGCGC TCCGAGGACC TCTrTrTAi CGAGATGAACTGGCAGAAGiWATTCGAG6CAGCCTGTCT TGTACTGGtGTGCCCGAACATGTCTT TCTGGAGCAGCiTCTCCTTSCAiCCTGGCCGTCCTGATGAACC TG,CTGGTGGC, GrTTTTTCTAfCCATTTGUr.TGAGCGG,GAACAiCTAGCCACACtGGTCAGGCCtCCTGTGGACiGCATGCTCiTCTCTCTGGCCAT TGTCAT TGC TCTGCcciArccCCACGGd
CATCCGGGCC;TTAATTGCTtCTACAATCCtACGACTGATiTT TTCAG T TGCTTGCAGC6CACCTGTTtCTGCTGGCAGCTT TCAATGtC TGCAATAAiATCATCTTCCTGATGArGCTT TGTG(GCCAACTGTGGGACCtTCACCAGACGdCTACCGGGCCATGGTTCTGGATGTGGACTtCCTCTATCAtTTTGCTGTATCTACTCATC TGTGCCATGGGCCTCT TCGTACATGAGT TCT T CTATAGCT TGTGCTTTTTGATTTAGTGTiCAGAGA,GGAGACT TTGCTTiATGTCATTAGMTGTCACCCGAATGGACGGTCCATCATCT TGACACCGGTCC TGGCTCTGATCCTGG T TTACCTGT TCTCAATTGTGGGCTATC TGTTCT TCAAGGAtGACTTTATCtTGGAAGTAGiTArCGTTGcrCCAATGAACAGCTGTTCCAGi^ACTGGCGAGATTrTGGCCiACGAT TTCCT GTACTC TGATGTGTGCAGGGTAGAGACGG6GGAGAACTGCACCTCTCCTGCACCCAAAGiAGCTGCT6CCTrGCCGA^GAAACGGAACkGGATAAGG^ACACACGTGT6AGACCCTGC r
CATGTGCATcGTCACTGTTcTGAGTCACGGGcTGCGGAGtGGGGGAGGGC,TArGAGAcGtGCTCAGGAAdccATC,cAAAGGAGCCTC fGTTTG;C TGCiAGrGGTGA TCtACGACCTCC r CT TCT TCTTCATGGTCATCiTCATCGTCCTGAACCTGATtTTCoGGGGTCiTCATCGACACCTTTGCTGACTGAGGAGTAGAAbGCAAAAiGAAGGAGGAGATCTSTAAAAiCCACGTGCT T CATC TGCGGCTTGGAAACGGAC;AAGTTTGACAATAGC GTCACCT TTGAAGAGCACATCAAGGAAGAACACAACATGTGGCACTATCTGTGCTT CATrCGTGCTrGGTGAAAGTrGAAGGA CTCCACAGAGTACACCGGG6CCTGAGAGT TiCGTGGCAGA6AGATCWGAACAGAAACCtT ATTGGT TCCTCAGAATGiGArGCCATGT6CCCTGGTCAGCArGCGAT TC TGAAGGGGAACi GAACGAGCTWGAACCTGCAGGAGAAGCtGGAGTCTACCATGAACGC TGrTCACCAATCtT TCTGGCCAGC TGTCAGAACTAAAGGACC iGATGACAGAiCAGAGGAAGCAGAAACAAAG AATCGGCCTfCTAGGACATCCTCCTrCACAtGAATGTCAACCCACAGCAGCCGGCCTAGCCAAATGArGGCiGAGGGACTCtGCTCAGCCCtC TGTATATCiC TGTCAGGGtGGGTACGGC T CAT TGCT TCfGAT TTGCCCiCTAAGGGTACATGTGCGCT tAGTACAT T TTAAATACTCiGTTrTT GTATtfGTATGTATAtiGA TTGC TATiC TCAGAGGT tTGGAC TTTCGTA TTGT AAT t AGCTCTGT T6GCATGGTGACTTGTCACTCCTGCCAAAAAtATTAAAAATGCCTTTTTTGGoGGACTACiGAAAGTACCtGAT TTGCACtTGAACCAGAtTATAGAT TTiAAAGTATATG ACATGTAT TtTGTATT T&AiACTAGAATA6CCAGTATTTiTGTT TTT TAtAAAAC TGTC-AATACAAATtATGCAATCACCAT AAC T T TTAAC TCC TGiG TGTCC TAGAGGAGTACACi TcTTTc^GAc,TGA rTTTGTTGATACTCGTGt AATAAATGGi TAAATATCAiATGCTGC TGC TGC JGCCAAAATTATAT TAiTAGCGAGTTtfC TGrGCCCCTGGGCAAT T T TTACCT TGT Ai TTATCC TATdGTGATGCTGtTTCTCGTT GcTAATGGCAT fAGTGCCCC TGTATCCT AGTGATAACTCCAGGTCTG TGAA&CA^TTCAAACiGCA TTCAT T tTGAGAAAAGCAAC T TTAGT f TCAAGGAT^AiTTTTAAGCTtCAAT TAAtCATTTAAAGtGTTTCTTTAAGAGAGCCATdTTAGAGGCT6ACACTTTAsC-TTGAAAr,GAGrTTGATGAATtAAT TT T TTAAAGG,GAACTT T T TACATGACGT TTGGAATA.iCAGCATAT TGC TGACCAGTCAG TGTCATC iCCCGGGTGAiTTTTr GATGTCACG TTATAGtCAAA TGAGT tAGC TGATGGtTrTC TAGAT T tTC TTCCTC TG AACCATrGA TGCAGTAGGTAiGAAGT TAT TiTGCGTATATiCATATATA^CiT TCATAArCGACAAAGTAGGAGCTGTCCCCrTTAGGA TGCi TAGC TGCCCCT AGrGGTACG TAGC TGAACAC TGACAATGGCGTTCTTCTGiAAGAGCCACGTTTGGGTTTtAT TTCTTTGiCACATGATTtCTTTTCTGGATGGG;TGCAAiGTATCACAGGAAGTGTTTTCTCTC TGTCGCCT TGTTTTGt ACCTGGGTCfCGCTTTACTiGACCGTCTCtGCACAAAAGtTTAAAAAC TGAACCGTATGCAGAGTTCCGiAGCAAGTCAiGT TTGTAAAtGCATACCTAAAAATAT TTAiTAAACGATGC AGAATCC TAAAAAAAAAAAAAAAAAAAAAAA
5520 5640 5760 5880 6000 6120 6240 6360 6480 6600 6720 6840 6960 7080 7200 7320
7440 7 560 7680 7800 7920 8040
8160 8280 8400
8520 8640 8 760 8880 9000
9120 9240 9 360 9480 9600 9720
9ss4
N., Niinobe, M., Nakahira, K., and Mikoshiba, K. (1988). J. Neurochem. 51, 1724-1730. Maeda, N., Niinobe, M., Inoue, Y., and Mikoshiba, K. (1989).
Dev.
5386
5040
998i1
References 1. Maeda,
3.
4080 4200 4320 4440 4560 4680 4800 4920
AATGAATGCATCCATGACCGAGTAT TCCiTGAAAGCATtCTGCTGGCCiTCAC TTCTGGAGGGC,CCCAAAACCiTrCAGCAcTCGT T TTTCT:CCTGCTAcAGAAGATAAGA ATCAGCAGWTTCTTCAACGdTTTTTTACGiTCGAATGAA6GTGGCCCAGCAGGAAATCAC=AGTG*ACATGTAACiCCAGCGACTtGGGAAACAAiAAGAAAGAT6AGAAG TGGi
2.
3 360 3480 3600 3720 3840 3960
Biol.
Furuichi,
133, 67-76.
T.,
et
al., submitted.
