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Control/Tracking Number: 2015-P-2535-ISSCR Activity: Poster Current Date/Time: 2/11/2015 2:13:09 PM DIFFERENTIAL ABILITIES OF DERMAL STEM CELLS DERIVED SCHWANN CELLS TO SUPPORT AXONAL REGENERATION AND REMYELINATION
Author Block: Kumar, Ranjan1, Sinha, Sarthak2, Stykel, Morgan1, Raharjo, Eko1, Raharjo, Eko1, Midha, Rajiv1, Biernaskie, Jeff1 1University of Calgary, Calgary, AB, Canada, 2University of Toronto, Toronto, ON, Canada
Abstract: Schwann cells (SCs) play a key role in supporting axonal regeneration and remyelination following a peripheral nerve injury. It is well known that outcomes following delayed nerve repair are poorer. Data suggests that in the chronically denervated nerve, SCs progressively lose their capacity to support axonal regeneration and may be less robust for remyelination. We hypothesized that recapitulating the early denervation phenotype of SCs in chronic denervation may restore remyelination and regeneration support capacity. In this study, we compared SCs from adult rodent sciatic nerve with acute and chronic denervation, adult rodent dermal stem cells derived precursor SCs (aSKP-SCs), and nerve derived SCs from E16 embryonic nerve. SCs re-express key pro-myelinating transcription factors (Oct-6 and Krox20) following acute (day 5) nerve injury, but lose this phenotype with chronic denervation (day 56) both in vivo and in cultured nerve SCs in vitro. We found that aSKP-SCs express Oct-6 and Krox-20, in vitro, to similar levels as the ones from acutely denervated nerve and significantly greatly than ones from chronically denervated nerve. We next tested and compared the various SCs for myelination both in vitro and in vivo and neurite outgrowth assay (DRG-SCs co-culture) in vitro. Additionally we compared aSKP-SCs and SCs for cellular proliferation. Adult SKP-SCs were comparable to acutely denervated nerve SCs or embryonic nerve SCs in terms of proliferation, survival in injured nerve, in vitro and in vivo myelination, and in vitro neurite outgrowth. Chronically denervated SCs were significantly poorer in all these capabilities. From this study we conclude that: 1) temporal delay following injury results in important phenotypic changes in distal Schwann cells within the nerve and 2) adult SKP-SCs can be used as an alternate therapy to restore myelination and promote axonal regeneration, in injured peripheral nerve, making these cells a favorable source of autologous cell transplantation. Travel Grant Justification: I have shown in depth dedication, mature judgment, team ethics and collaborative skills in forming three successful collaborations including university of Calgary (home), University of Alberta (Provincial) and University of British Columbia (inter provincial). During my trainee period I successfully learned and trained fellow trainees in stem cell isolation, expansion and to conduct functional studies. I have demonstrated outstanding qualities of leadership and professional skills and therefore, have been called upon to lead trainee outreach and education committee at home institution. Through my capable leadership I inspired fellow trainees for organizing workshops and stem cell talks. Abstract Submission Topic (Complete): Tissue Engineering, Organ Development and Regeneration ; Road to the Clinic Poster Presentation Category (Complete): *Poster Presentation Category: Regeneration Mechanisms
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Oasis, The Online Abstract Submission System
2015-02-11, 1:14 PM
Keywords (Complete): adult dermal stem cells ; autologous cell therapy ; nervous system repair Additional Information (Complete): *Does the research described in this abstract include human research participants?: No *Is the research described in this abstract part of an academic-industry partnership or collaboration?: No
Awards (Complete): *Would you like your submission to be considered?: Yes *I would like to apply for a 2015 travel award.: Yes Title: Trainee (student or post-doc)
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