Gel electrophoresis equipment (e.g., Mini-PROTEAN Tetra Cell [Bio-Rad] or XCell SureLock Mini-. Cell [Invitrogen]). Fluorescent imaging system (e.g., LI-COR ...
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A Rapid Western Blotting Protocol for the Xenopus Oocyte Yaping Lin-Moshier and Jonathan S. Marchant Cold Spring Harb Protoc 2013; doi: 10.1101/pdb.prot072793 Email Alerting Service Subject Categories
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Protocol
A Rapid Western Blotting Protocol for the Xenopus Oocyte Yaping Lin-Moshier and Jonathan S. Marchant1 Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455
Often experimentalists require a quantitative assessment of the levels of heterologously expressed proteins to best interpret changed Ca2+ signaling patterns. Here, we detail a rapid and convenient western blotting method for individual Xenopus oocytes. The method exploits recently introduced rapid blotting systems, commercially available from Invitrogen (iBlot) or Bio-Rad (Trans-Blot Turbo). The key advantage is speed: from live cell to transferred membrane in
