PCR PE PE PCR PE PE - PLOS

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ul PCR in the PE. 1.0 al PCR in the PE. 0.125. 4.0. 2.0. 1.0. 0.5. 0.25. PE. 217 PE. 217. PE. 217. M-1b: S-3a : X-la. M-2b : S-7a:J-5a. M-1b : S-7a : J-5a.
0.125

0.25

0.5

M

1.0

4.0

A

2.0

µl Bulk DNA in the PCR

C

PCR Serial dilutions of the PCR (1.0 µl)

PE

0.125

0.5

1.0

1.0 µl PCR in the PE

2.0

4.0

B

0.25

µl PCR in the PE

PE

217

217

D

S-7a/J-5a

Serial dilutions of the PCR (0.25 pg)

E. 1.0µl 0.5µl 0.25µl 0.125µl 0.0625µl 1.0µl 0.5µl 0.25µl 0.125µl 0.0625µl

0.25pg

0.5pg

1.0pg

2.0pg

4.0pg

0.25pg

0.5pg

1.0pg

4.0pg 2.0pg 1.0pg 0.5pg 0.25pg M-1b/J-5a

M-2b : J-ia

2.0pg

M-2b : X-1a + Plasmid1 (tagM/Jδ1/Jβ2.7)

M-2b : S-8a : C-3b + Plasmid1 : Plasmid2 (tagM/Jδ1/tagC) (tagM/Dδ3/tagS2)

4.0pg 2.0pg 1.0pg 0.5pg 0.25pg

0.25pg

0.5pg

1.0pg

4.0pg

0.25pg

0.5pg

1.0pg

M-1b : S-7a : J-5a + Plasmid1 : Plasmid2 (tagM/Jβ2.7) (tagS2/Jβ2.7)

S-7a/J-5a M-2b/J-5a

S-3a/X-1a

4.0pg

4.0pg

2.0pg

M-1b/X-1a

2.0pg

M-2b : S-7a : J-5a + Plasmid1 : Plasmid2 (tagM/Jβ2.7) (tagS2/Jβ2.7)

M-1b : S-3a : X-1a + Plasmid1 : Plasmid2 (tagM/Jβ2.7) (tagS1/Jβ2.7)

M-2b* S-8a*

M-2b/C-3b M-2b/S-8a