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Persoonia – Volume 36, 2016
Penidiellopsis radicularis
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Fungal Planet description sheets
Fungal Planet 460 – 4 July 2016
Penidiellopsis Sandoval-Denis, Gené, Deanna A. Sutton & Guarro, gen. nov. Etymology. Named after its morphological resemblance to the genus Penidiella.
Classification — Teratosphaeriaceae, Capnodiales, Dothi deomycetes. Conidiophores differentiated, solitary, erect, straight to geniculate-sinuous, rarely branched, pale to medium brown, smoothand thick-walled. Conidiogenous cells integrated, terminal or intercalary, pale to medium brown, smooth, mono- and polyblastic, giving rise to one or more sets of ramoconidia, scars truncate, slightly darkened, unthickened and not refractive. Ramoconidia 0 –1-septate, obovoid, ellipsoid or slightly
clavate, pale to medium brown, smooth- and thick-walled, apical part with denticle-like loci, basal scar flattened, slightly darkened, unthickened and not refractive. Conidia in branched acropetal chains, 0-septate, obovoid, ellipsoid or limoniform, pale to medium brown, smooth, thick-walled, with conidial scars truncate or protuberant, somewhat darkened, unthickened and not refractive. Type species. Penidiellopsis radicularis Sandoval-Denis, Gené, Deanna A. Sutton & Guarro. MycoBank MB815361.
Penidiellopsis radicularis Sandoval-Denis, Gené, Deanna A. Sutton & Guarro, sp. nov. Etymology. Named after its root-like growth pattern in culture media.
Mycelium superficial and immersed, composed of septate, branched, pale brown, smooth to finely verruculose and thinwalled hyphae, 1.5 – 4.5 μm wide. Conidiophores straight or geniculate, septate, slightly constricted at the septum, 40–150 × 3.5– 5 μm, pale to medium-brown, usually darkening at the medial portion, smooth-, thick-walled. Conidiogenous cells terminal or intercalary, 12–18 × 4–5 μm, with one or several conidiogenous loci, 1– 2.5 μm wide. Ramoconidia 0(–1)-septate, ellipsoid, obovoid or somewhat clavate, 7–11 × 3 – 4 μm, pale to medium brown, smooth and thick-walled. Conidia 0-septate, obovoid or limoniform, 5–9 × 3–4 μm, pale brown, smooth and thick-walled, with protuberant conidial scars. Culture characteristics — (in the dark, 25 °C after 14 d), colonies on PDA attaining 6–7 mm diam, dark green (30F3/F8) (Kornerup & Wanscher 1978), erumpent and folded, velvety; reverse dark green (30F3) to black. On SNA attaining 4–8 mm diam, olive grey to olive (3F2/F8), flat, velvety; reverse olive (3F7/F8). On OA attaining 6 – 8 mm diam, dark green (30F8) to black, flat, velvety; reverse dark green (30F8). Typus. USA, South Carolina, West Columbia, from human nail, date unknown, D.A. Sutton (holotype CBS H-22389, culture ex-type CBS 140695 = UTHSC DI-13-256 = FMR 13369; ITS sequence GenBank LN834441, LSU sequence GenBank LN834445, MycoBank MB815362).
Notes — The genus Penidiellopsis (Ps.) is similar to Peni diella (Pa.), however, both genera are clearly differentiated genetically and morphologically. While Penidiella produces penicillate branched conidiophores, those of Penidiellopsis are mostly unbranched, although its conidial chains exhibit a continuous bi- or trifurcating elongation pattern (Crous et al. 2007a). The monotypic genus Xenopenidiella exhibits also similar morphological features, however, is genetically distinct and produces dimorphic conidiophores with loosely branched apices (Quaed vlieg et al. 2014). Our phylogenetic results showed that Ps. radicularis is closely related to Pa. aggregata and Pa. drakensbergensis, two species not included in Penidiella s.str. (sensu Quaedvlieg et al. 2014) (for phylogenetic tree, see MycoBank). However, the new genus Penidiellopsis is genetically well-delimited, and also differs from the latter two species by its shorter ramoconidia (vs 8–15 μm and 10–15 μm long in Pa. aggregata and Pa. drakensbergensis, respectively) and its wider, aseptate intermediate and terminal conidia. Based on a megablast search of NCBIs GenBank nucleotide database, the closest hit using the ITS sequence is Pa. aggre gata CBS 128772 (GenBank JF499842; Identities= 466/508 (92 %), Gaps = 11/508 (2 %)), followed by Pa. drakensbergen sis CPC 19778 (GenBank NR_111821; Identities = 469/519 (90 %), Gaps = 23/519 (4 %)) and Teratosphaeria agapanthi CBS 129064 (GenBank JF770456; Identities = 435/509 (89 %), Gaps= 18/509 (3 %)). Closest hits using the LSU sequence were to Pa. aggregata CBS 128772 (GenBank JF499862; Identities = 539/551 (98 %), Gaps = 0/551 (0 %)), Pa. drakensberg ensis CPC 19778 (GenBank KC005792; Identities = 536/551 (97 %), Gaps = 0/551 (0 %)) and Teratosphaeria macowanii CPC 1872 (GenBank EU019254; Identities = 534/551 (97 %), Gaps = 0/551 (0 %)).
Colour illustrations. USA, South Carolina, view of the Gervais Street Bridge (image credit: Wikimedia commons); conidiophores, conidiogenous cell bearing conidia in branched chains. Scale bars = 5 µm.
Marcelo Sandoval-Denis, Josepa Gené & Josep Guarro, Mycology Unit, Medical School and IISPV, Universitat Rovira i Virgili (URV), Sant Llorenç 21, 43201 Reus, Tarragona, Spain; e-mail:
[email protected],
[email protected] &
[email protected]. Deanna A. Sutton & Nathan P. Wiederhold, Fungus Testing Laboratory, Department of Pathology, University of Texas Health Science Center, 7703 Floyd Curl Dr., San Antonio, Texas 78229-3900, USA; e-mail:
[email protected] &
[email protected] © 2016 Naturalis Biodiversity Center & Centraalbureau voor Schimmelcultures
