References. 1) Baecher-Allan, C., Brown, J.A., Freeman, G.J., and Hafler,. D.A. 2001. CD4 CD25HIGH regulatory cells in human peripheral blood. J. Immunol.
Microbiol. Immunol., 50(8), 597–605, 2006
Peripheral Blood Mononuclear Cells Immunophenotyping in Pulmonary Tuberculosis Patients before and after Treatment Warly Barcelos1, Olindo Assis Martins-Filho2, Tânia Mara Pinto Dabés Guimarães1, Márcio Hamilton Prostzner Oliveira3, Silvana Spíndola-de-Miranda4, Beatriz Nascimento Carvalho5, and Vicente de Paulo Coelho Peixoto de Toledo*, 1 1
Departamento de Análises Clínicas e Toxicológicas, Faculdade de Farmácia da UFMG, Belo Horizonte, MG, Brasil, 2Centro de Pesquisas René Rachou/FIOCRUZ, Belo Horizonte, MG, Brasil, 3Centro de Saúde Oswaldo Cruz/ Prefeitura de Belo Horizonte, MG, Brasil, 4Departamento de Clínica Médica, Faculdade de Medicina da UFMG, Belo Horizonte, MG, Brasil, and 5 Fundação Centro de Hematologia e Hemoterapia de Minas Gerais (HEMOMINAS, Belo Horizonte, MG, Brasil) Received December 15, 2005; in revised form, April 17, 2006. Accepted May 8, 2006
Abstract: Tuberculosis (TB) is a lung disease caused by Mycobacterium tuberculosis. The interaction between the bacillus and the host may lead to a protective cellular immune response. In the present study, we propose the “in vitro” evaluation of this cellular immune response in patients with tuberculosis before and after chemotherapic treatment. Eleven patients with TB and 9 asymptomatic subjects with tuberculin skin test negative (TST) (purified protein derivative (PPD) ≤10 mm) were evaluated. The peripheral lymphocytes of the subjects were analyzed utilizing the following surface markers: CD3, CD4, CD8, CD19, CD25, CD56, CD14, CD16 and HLA-DR. At the end of the treatment, symptomatic patients presented a significant predominance (P
