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Sep 13, 2007 - Preterm Birth without Progesterone Withdrawal in. 15-Hydroxyprostaglandin Dehydrogenase. Hypomorphic Mice. Jeffrey D. Roizen, Minoru ...
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Molecular Endocrinology 22(1):105–112 Copyright © 2008 by The Endocrine Society doi: 10.1210/me.2007-0178

Preterm Birth without Progesterone Withdrawal in 15-Hydroxyprostaglandin Dehydrogenase Hypomorphic Mice Jeffrey D. Roizen, Minoru Asada, Min Tong, Hsin-Hsiung Tai, and Louis J. Muglia Departments of Pediatrics and Obstetrics and Gynecology and Center for Preterm Birth Research (J.D.R., M.A., L.J.M.), Washington University School of Medicine, St. Louis, Missouri 63110; and Department of Pharmaceutical Sciences (M.T., H.-H.T.), College of Pharmacy, University of Kentucky, Lexington, Kentucky 40536 Parturition is a complex mammalian physiological process whose fundamental determinants have remained elusive. The increasing incidence of human preterm birth, a leading cause of infant mortality, highlights the importance of further understanding mechanisms regulating the timing of birth. Parturition is initiated in most nonprimate mammals, including mice, through a decrease in circulating progesterone caused by elevated prostaglandins. In humans, other higher primates, and guinea pigs, no consistent decrease in circulating progesterone occurs before the onset of labor. The divergence in endocrine control of labor initiation between most mammals compared with the great apes and guinea pigs gives rise to the question: how could a mechanism for the initiation of labor not requiring the withdrawal of progesterone evolve? Here, we

genetically modulate prostaglandin signaling to determine the role of prostaglandin catabolism in the timing of birth. We find spontaneous preterm labor in the absence of progesterone withdrawal in 15-hydroxyprostaglandin dehydrogenase hypomorphic mice. The onset of labor in these hypomorphic mice is preceded by prematurely increased concentrations of prostaglandin E2 and F2␣. Moreover, genetic crosses demonstrate a role for fetal genotype in birth timing. Together, these findings demonstrate a 15-hydroxyprostaglandin dehydrogenase-dependent shift in the physiology of murine parturition to one resembling the physiology of higher primates. Thus, endocrine control of labor has the capacity to plastically adapt to changes in genetically determined prostaglandin signals. (Molecular Endocrinology 22: 105–112, 2008)

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initiated by a decrease in circulating progesterone (progesterone withdrawal) (3–5). However, in the great apes, including the human, no such decrease occurs before normal labor (6, 7). Despite the difference in regulation of progesterone concentrations, myometrial biochemical changes associated with increased contractility are conserved across all mammalian species. This conservation of the final pathway for myometrial activation has been suggested to reflect that functional progesterone withdrawal occurs in humans to initiate labor (8–10). Alternatively, other pathways conserved across mammals, such as prostaglandins (PGs) (11), may supercede the necessity for progesterone withdrawal. In a range of species, PGE2 and PGF2␣ facilitate labor as contractile agonists (11). In rodents, PGF2␣ has an additional role in initiating labor by stimulating luteolysis and progesterone withdrawal (12, 13). The enzyme primarily responsible for the breakdown of PGE2 and PGF2␣ is 15-hydroxyprostaglandin dehydrogenase (15-HPGD) (14, 15). The regulation of PG breakdown rather than synthesis as an important determinant of labor onset has been suggested by several observations. In humans, a decrease in 15-HPGD mRNA and activity in the chorion trophoblast cells in laboring women relative to nonlaboring women at term has been measured (16). This difference is further ac-

RETERM LABOR OCCURS in approximately 12% of births in the United States and is the chief cause of neonatal morbidity and mortality (1). Of substantial concern, annual rates of prematurity are increasing. Intervention focused on preventing preterm labor would be an ideal strategy to address this problem, but the mechanisms underlying the timing of normal labor and the pathogenesis of spontaneous idiopathic preterm labor remain unknown (2). Understanding the mechanism determining the timing of labor onset presents a particularly difficult problem in that it likely involves both redundant signaling mechanisms and integration of input from two distinct genetic components, the mother and the fetus. Moreover, extrapolation of mechanisms from animal model systems to humans has proven problematic. In most mammalian species aside from the great apes, labor is First Published Online September 13, 2007 Abbreviations: dpc, Day post coitus; EP receptor, PGE2 receptor; ES, embryonic stem; FP receptor; PGF2␣ receptor; 15-HPGD, 15-hydroxyprostaglandin; 20␣-HSD, 20␣-hydroxysteroid dehydrogenase; PG, prostaglandin; RMSE, root mean squared error. Molecular Endocrinology is published monthly by The Endocrine Society (http://www.endo-society.org), the foremost professional society serving the endocrine community. 105

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we generated and analyzed 15-HPGD hypomorphic (HpgdH/H) mice.

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Roizen et al. • Preterm Birth in 15-HPGD Hypomorphic Mice

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The 15-HPGD hypomorphic allele (HpgdH) arose as a result of an aberrant homologous recombination event resulting in tandem duplication of the 5⬘ end of the Hpgd gene along with insertion of neomycin resistance cassettes in intronic regions (Fig. 1A). To define the consequences of this integration event on Hgpd gene transcription and activity, we examined 15HPGD mRNA by Northern blot (Fig. 1, B and C) and in situ hybridization (Fig. 2) and performed activity assays (Fig. 3). 15-HPGD mRNA abundance was measured in the tissues expressing the highest levels of this enzyme: the liver, lung, and placenta (15). In all of these tissues, HpgdH/H mice have a significant decrease in 15-HGPD mRNA relative to control mice (Fig. 1, B and C). In day post coitus (dpc)-17.5 implantation site and fetus, 15-HPGD in situ mRNA hybridization further highlights the developing lung, liver, and placenta in the wild-type mice as tissues of high 15HPGD expression, with reduced mRNA expression in the HpgdH/H mice (Fig. 2). In both the adult liver and lung, HpgdH/H mice have a significant decrease in PG dehydrogenase activity relative to wild-type mice (Fig. 3A). We also measured PG dehydrogenase activity in the placenta, uterus, and fetal membranes (Fig. 3, B–D) because of their importance in PG production and metabolism during gestation. On dpc 11.5, each of these tissues from HpgdH/H matings demonstrate a significant decrease in 15-HPGD activity relative to control tissues. On dpc 17.5, HpgdH/H placenta and uterus, but not fetal membranes, display a significant decrease in 15-HPGD activity relative to the same wild-type control tissue. In wild-type pregnancy, 15-HPGD activity decreases significantly to activity levels similar to those observed in the hypomorphic gestational tissues at dpc 18.25 and 19.0 (Fig. 3, B–D). To define the consequences of this alteration in 15-HPGD activity on birth timing, we compared the length of gestation in pregnancies arising after mating

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Fig. 1. Presence of 15-HPGD Hypomorphic Alleles Results in Decreased 15-HPGD mRNA Gene Expression A, Wild-type (WT) Hpgd⫹/⫹ and hypomorphic HpgdH/H alleles; B, Northern blot analysis of 15-HPGD mRNA in the adult lung, liver, and dpc 15.5 placenta, with cyclophilin mRNA detection provided as loading control; C, quantification of differences in 15-HPGD mRNA abundance in the adult lung, liver, and dpc 15.5 placenta normalized to wild-type values. Values are mean ⫾ SEM. ***, P ⬍ 0.001; *, P ⬍ 0.05; n ⫽ 3.

centuated in women who are laboring preterm (16, 17). Analogously, in a mouse model of bacterial-induced preterm labor, induction of labor correlates with a decrease in 15-HPGD mRNA in the fetal membranes and the fetus (18). To test the hypothesis that 15-HPGDmediated PG breakdown regulates the timing of labor,

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Fig. 2. 15-HPGD mRNA Abundance Is Decreased in the dpc 17.5 15-HPGD Hypomorphic Implantation Site and Fetus In situ hybridization demonstrates abundant 15-HPGD mRNA expression in wild-type (WT) but not hypomorphic (Hypo) fetus and surrounding structures. H&E, Hematoxylin and eosin stain of an adjacent section; Li, liver; Lu, lung; Plac, placenta; U, uterus.

Roizen et al. • Preterm Birth in 15-HPGD Hypomorphic Mice

Fig. 3. Presence of 15-HPGD Hypomorphic Alleles Results in Decreased 15-HPGD Activity The 15-HPGD hypomorphic alleles decrease 15-HPGD activity in adult lung and liver (A), dpc 11.5 fetal membranes (B), dpc 11.5 and 17.5 uterus (C), and dpc 11.5 and 17.5 placenta (D) compared with wild-type mice. 15-HPGD activity significantly decreases after dpc 17.5 in wild-type mice compared with dpc 11.5 in wild-type mice in fetal membranes (B), uterus (C), and placenta (D). Values are mean ⫾ SEM. ***, P ⬍ 0.001; **, P ⬍ 0.01; *, P ⬍ 0.05; n ⫽ 3–4. Measurements at dpc 18.25 do not differ between genotypes, and only wild-type samples are measured at dpc 19.0.

hypomorphic mice and wild-type littermates (Fig. 4). When both the female and male are hypomorphic (HpgdH/H), labor occurs more than half a day early relative to wild-type females (Hpgd⫹/⫹) mated with the HpgdH/H males (Fig. 4A; P ⬍ 0.05) and almost a full day early relative to wild-type pregnancies (Hpgd⫹/⫹⫻

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Hpgd⫹/⫹, Fig. 4B; P ⬍ 0.05). However, when HpgdH/H females are mated with wild-type (Hpgd⫹/⫹) males, they do not labor significantly early relative to wildtype (Hpgd⫹/⫹) females mated with the same males. Multiple regression was used to examine the relative contributions of maternal genotype and fetal genotype on the timing of birth in these matings. Both maternal and fetal genotypes each have a statistically significant effect on the timing of labor. Maternal genotype when examined alone was found to be statistically significantly predictive for the timing of labor [P ⫽ 0.0002; r2 ⫽ 0.28; root mean squared error (RMSE) ⫽ 0.4252]. Similarly, fetal genotype when examined alone was found to be statistically significantly predictive for the timing of labor (P ⬍ 0.0001; r2 ⫽ 0.42; RMSE ⫽ 0.3886). However, when both maternal and fetal genotypes are included in the regression model, only fetal genotype demonstrated a statistically significant effect on the timing of labor (whole model predicted P ⬍ 0.0001; r2 ⫽ 0.42; RMSE ⫽ 0.3919; maternal genotype leverage predictive P ⫽ 0.73; fetal genotype leverage predictive P ⫽ 0.01). There was no significant effect of maternal or paternal genotype on pup survival (data not shown). Because PGF2␣ is primarily inactivated by 15-HPGD (19), and PGF2␣ has a known prominent role in the induction of labor in mice (13), the concentration of PGF2␣ was compared in gestational tissues between hypomorphic pregnancies and wild-type pregnancies (Fig. 5, A–C). PGF2␣ concentrations were significantly elevated in hypomorphic mice relative to the wild-type mice in the uterus and placenta at dpc 17.5 and in the uterus at dpc 18.25. Additionally, PGF2␣ concentrations were significantly elevated in hypomorphic mice at dpc 17.5 in the uterus and ovaries and in dpc 18.25 uterus relative to hypomorphic mice at dpc 15.5. Normally, in mouse pregnancy, signaling of high PGF2␣ levels through ovarian corpus luteal PGF2␣ receptors (FP receptors) cause the initiation of labor by inducing luteolysis and a decrease in circulating progesterone (12, 13). To determine whether this mechanism underlies the early labor in 15-HPGD hypomorphic mice, serum progesterone was measured during the last several days of pregnancy from hypomorphic (HpgdH/H⫻ HpgdH/H) and wild-type (Hpgd⫹/⫹⫻ Hpgd⫹/⫹) pregnancies (Fig. 6A). The gravid wild-type females exhibit a progressive fall in serum progesterone as labor approaches similar to what has been observed in inbred C57BL/6 mice previously (20). In contrast, the hypomorphic mice show no decrease in maternal serum progesterone before labor. We also measured serum ␤-estradiol in hypomorphic and control pregnancies at 17.5 (HpgdH/H 7.5 ⫾ 2.9 pg/ml vs. Hpgd⫹/⫹ 9.3 ⫾ 1.0 pg/ml; n ⫽ 3) and 18.25 (HpgdH/H 10.3 ⫾ 1.8 pg/ml vs. Hpgd⫹/⫹ 5.0 ⫾ 1.7 pg/ml; n ⫽ 3) dpc and found no significant differences. These surprising results prompted us to determine how the corpus luteum is maintained in the context of high PGF2␣ concentrations. We measured the abundance of the other PG metabolized by 15-HPGD,

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Roizen et al. • Preterm Birth in 15-HPGD Hypomorphic Mice

Fig. 4. 15-HPGD Hypomorphic Pregnancies Labor Early Relative to Wild-Type Pregnancies A, Gestation length after mating with a 15-HPGD hypomorphic male; B, gestation length after mating with a wild-type (WT) male. Values are mean ⫾ SEM. *, P ⬍ 0.05 relative to mating between a 15-HPGD female with a wild-type male; n ⫽ 7, except n ⫽ 14 for wild-type male ⫻ female, and n ⫽ 15 for 15-HPGD hypomorphic homozygous (homo) male ⫻ female. Het, Heterozygous.

PGE2, which has been shown to have luteotropic actions and oppose PGF2␣ luteolytic effects (21, 22). PGE2 concentration was significantly elevated in the placenta and ovary of hypomorphic pregnancies at dpc 17.5 and 18.25 (Fig. 6, B and C). Thus, it is likely that the maintenance of the corpus luteum in the context of high PGF2␣ occurs because of increased trophic effects of PGE2. In mice, high PGF2␣ levels cause the initiation of labor by inducing expression of 20␣-hydroxysteroid dehydrogenase (20␣-HSD) (23, 24) in the corpus luteum leading to luteolysis and a decrease in circulating progesterone concentration. Consistent with the hypothesis that elevated PGE2 maintains ovarian progesterone secretion, we find that despite elevation in ovarian PGF2␣ concentration in the hypomorphic females at dpc 17.5, no induction of 20␣-HSD mRNA occurs (Fig. 7A). This result is in contrast to the robust induction of 20␣-HSD mRNA that wild-type females display at dpc 19.0, when progesterone levels are falling just before labor. Consistent with the failure to induce 20␣-HSD mRNA, we found no histological evidence for luteolysis in the hypomorphic ovaries at dpc 18.25, with corpora lutea demonstrating prominent vascular spaces, uniform cellular appearance, and organized architecture (Fig. 7B) as opposed to those changes reported in wild-type mice (25).

DISCUSSION Although several genetically altered murine models exhibit prolonged or delayed labor (12), 15-HPGD hypomorphic mice are the first genetic murine system to exhibit spontaneous preterm labor. This early labor is not entirely unexpected; 15-HPGD is known to have a critical role in metabolizing PGF2␣, a PG that causes the initiation of labor through its luteolytic action on the ovaries (13) and directly promotes uterine contractions

(11). The decrease in HPGD activity we find in wildtype control placenta, fetal membranes, and uterus late in gestation suggests that down-regulation of HPGD normally occurs to enhance paracrine and more distant actions of PGs. Because HPGD activity is low from at least dpc 11.5, the delay in parturition in the Hpgd⫹/⫹ mice to later in pregnancy indicates that the induction of PG synthesis is also an important determinant for parturition timing. In the uterus, induction of cyclooxygenase-1 mRNA and protein has been found to precede the time at which the Hpgd⫹/⫹ mice deliver their pups (20, 25), consistent with the pattern of tissue PGs that we measure. However, the maintenance of circulating maternal progesterone concentration in association with the early labor was unexpected. Before this study, another group reported the creation of mice completely lacking 15-HPGD activity (26); however, the labor phenotype in these mice was not described. Unlike our hypomorphic line, these conventional knockout mice die in the perinatal period because of failure to close the ductus arteriosus. We have also generated a complete knockout HPGD line and find similar perinatal mortality (Roizen, J. D., and L. J. Muglia, unpublished data). After indomethacin rescue, mating of HPGD KO males and females results in no pregnancies that last beyond the first week of gestation (Roizen, J. D., and L. J. Muglia, unpublished data), making the current hypomorphic model particularly valuable. Future studies to further mechanistically link augmented uterine PG concentration with the onset of labor in the absence of progesterone withdrawal should be greatly facilitated by the hypomorphic line. Detailed definition of the mechanism of myometrial activation in 15-HPGD hypomorphic mice will offer new insights into how term and preterm labor are initiated in humans and mechanisms of evolution of parturition control in general. Our findings also implicate the utility of evaluating genetic variation in the human HGPD gene as contributing to the risk of human preterm birth.

Roizen et al. • Preterm Birth in 15-HPGD Hypomorphic Mice

Fig. 5. Presence of 15-HPGD Hypomorphic Alleles Leads to Increased Concentrations of PGF2␣ 15-HPGD hypomorphic pregnancies have increased concentrations of PGF2␣ in the placenta (A), uterus (B), and ovary (C) on dpc 17.5 and dpc 18.25 (uterus only) relative to control mice. HpgdH/H ovarian PGF2␣ concentration is significantly increased at dpc 17.5 in comparison with dpc 15.5. Values are mean ⫾ SEM. ***, P ⬍ 0.001; **, P ⬍ 0.01; *, P ⬍ 0.05; n ⫽ 4–5.

The observation of the early initiation of labor in the context of sustained circulating progesterone in the 15HPGD hypomorphic mice is notable in light of the delayed labor observed in the FP receptor knockout mice (13). In the FP receptor knockout mice, labor is delayed due to maintained circulating progesterone. In these mice, there is no evidence of elevated PGE2, however. Although FP receptor activation by its ligand has effects that uniformly accelerate the timing for birth by promoting myometrial contraction and luteolysis, the consequences of PGE2 actions on its receptors (EP receptors) is more complex. In the myometrium, action on the EP2 and EP4 receptors cause myometrial relaxation, whereas action on EP1 and EP3 promote myometrial contraction (27). Both normal gestational changes in relative expres-

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sion of the FP and EP isoforms in the myometrium, along with changes that may result from the altered PG and progesterone milieu in our hypomorphic line, likely contribute to the timing for preterm birth in the context of high circulating serum progesterone levels. Our results suggest that the increased concentration of PGE2 arising from the placenta may prepare the HpgdH/H uterus for contractions and cause early labor in the setting of maintained circulating progesterone. Alternatively, high concentrations of PGF2␣ may act via the FP receptor to prepare the uterus for labor even with high progesterone levels, a uterine activity that would not be present in FP receptor knockout mice. This mechanism is also possible because uterine PGF2␣ is elevated at dpc 17.5 and 18.25 in the gravid HpgdH/H mice. The profile of PGE2 induction in the HpgdH/H pregnancy further shifts the normal pattern of murine pregnancy to one resembling human pregnancy. Previous studies have shown increased expression of cyclooxygenase isoforms in the placenta as parturition approaches in mice (28), likely accounting for the steady increase in PGE2 that we find in this tissue in the hypomorphic line. The high expression of HPGD in wild-type mice prevents this increase in PGE2 as is demonstrated in unchanged PGE2 in wild-type placenta through dpc 18.25, as we found. The reprogramming of rodent pregnancy from a PGF2␣-progesterone-withdrawal system to a PGE2-no-progesterone-withdrawal system suggests that higher primate parturition could have evolved in a one-step process, because an integrated regulatory network already exists to use such a pathway. In addition to initiating labor in the absence of a decrease in circulating progesterone, the HpgdH/H mice reveal an important role for the fetus in the timing of murine parturition. Hypomorphic females labor significantly early only if their pups are also hypomorphs. Classic studies have previously revealed an important role for the fetal hypothalamic-pituitary-adrenal axis in labor initiation in sheep (29), and recent studies have suggested a novel hormonal role for surfactant apoprotein-A arising from the fetus in contributing to labor initiation in mice (30). The balance of PG signaling during gestation, in contrast to the proposed role of fetal adrenal activity or surfactant apoprotein-A, involves opposing functions of maternal and fetal systems. Previous studies in cycloxygenase-1 (25) or cytosolic phospholipase A2 (31) knockout mice demonstrated that the mother is primarily responsible for the generation of PGs, hastening the onset of labor. We show that the fetus (or fetally derived extraembryonic tissues) has a significant role in breaking down PGs and delaying labor. Such results harmonize well with the current understanding of the relative evolutionary benefits to the mother and the fetus of alterations in the timing of labor (32). Delays in the timing of labor are advantageous to the fetus relative to the mother as a delay increases the chance of further organ and cognitive maturation at the time of delivery, whereas early labor is advantageous to the mother in that it enables

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Roizen et al. • Preterm Birth in 15-HPGD Hypomorphic Mice

Fig. 6. Measurement of Serum Progesterone and Tissue PGE2 in 15-HPGD Hypomorphic Pregnancies A, Serum progesterone concentration in hypomorphic and wild-type pregnancies displayed as a function of days before labor onset. Values are mean ⫾ SEM. *, P ⬍ 0.05; n ⫽ 3 for all, except n ⫽ 5 for hypomorphic pregnancies one half day before labor. B, PGE2 concentrations in the placenta. *, P ⬍ 0.05 compared with all other measures; n ⫽ 4. C, PGE2 concentration in ovary. *, P ⬍ 0.05; n ⫽ 4.

her to more easily deliver a smaller fetus, conserve nutrients, and better protect herself from environmental stressors.

MATERIALS AND METHODS Generation of HpgdH/H Mice To construct the conditional 15-HPGD targeting vector, loxP sites were inserted into unique restriction sites up-

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