Production of an Inhibitor of Rat Mesangial Cell the Glomerulus and Its Alteration in Puromycin Neph rosis1’2 Gerald
C. Groggel3
and
M. Laurette
(J. Am.
Soc.
Laurette Hughes, Department of Vermont, Burlington, VT
Nephrol.
1993;
by
Hughes
Impairment GO. Groggel, M. Medicine, University
Growth
of
be renal
4:155-161)
of mesangial
important
in
the
cell growth
regulation
pathogenesis
of
proilferation,
glomerulus
may
progressive
disease.
Key Words:
Mesangial
cell,
R
ABSTRACT Mesangial progressive due
cell proliferation renal disease.
to dysregulation
is found in many This proliferation
of mesangial
cell
forms of may be
growth.
The
studies presented here test the hypothesis that the normal glomerulus produces a regulator of mesangial cell growth. Conditioned media (CM) from primary glomerular cultures were able to inhibit rat mesangial cell growth in a dose- and time-dependent
fashion,
inhibition. pears
from
30.0
The growth
to have
± 3.8
to 86.6
inhibitor
a molecular
± 3.9%
growth
in glomerular
weight
CM ap-
of less than
3,000.
Glomerular CM caused significantly more growth inhibition than did 3T3 fibroblast CM, 77.9 ± 2.8% growth inhibition by 10% glomerular CM versus 21.2 5.4%
