Proteomic Forum 2015

www.proteomic-forum.de

Proteomic Forum 2015 Technical University Berlin 22–25 March 2015

Program

14. April 2015

6. Berliner LC /MS/MS Symposium 45 Fach-Referenten, 20 Industriepartner, 14 Fortbildungskurse, 5 applikationsspezifische Vortragsreihen, 1 schwimmender Chemieprofessor ...

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Table of Contents

3 Proteomic Forum 2015 

The German Society of Proteome Research

4

Welcome Note

5

Organization and Imprint

6

Index of Abstract IDs

7

Scientific Program

8

Program Overview

8

Sunday, 22 March 2015

12

Monday, 23 March 2015

17

Tuesday, 24 March 2015

21

Wednesday, 25 March 2015

25

Poster Sessions

29

Poster Session A – Sunday 22 March 2015

29

Poster Session B – Monday, 23 March 2015

38

Poster Session C – Tuesday, 24 March 2015

47

Lecture Abstracts of Young Investigator Award Session General Information

57–65 67

Poster Sessions Speakers’ Ready Room Requirements for Poster Awards and Prizes Travel Social Program

71

Sponsors and Media Cooperation

73

Exhibitors Plan

76

Author List (Session Chairs, Speakers and Presenting Authors)

78

4

DGPF

The German Society of Proteome Research Objectives of the Society The German Society of Proteome Research as organizer coordinates and provides a network comprising academic institutions, commercial companies and special institutions at the national and international level in order to support the extension of scientific knowledge in the field of proteome research and protein analysis. President: Albert Sickmann 1st Vice President: Uwe Völker 2nd Vice President: Marius Ueffing Recorder: Hans-Peter Braun Treasurer: Helmut E. Meyer Organizing Committee Ruedi Aebersold (Zurich/CH), Christoph Borchers (Victoria, CA), Hans-Peter Braun (Hannover/DE), Christian Huber (Salzburg/AT), Christian Kleinhammer (Graz/AT), George Tsangaris (Athens/GR), Karl Mechtler (Vienna/AT), Helmut E. Meyer (Bochum/DE), Albert Sickmann (Dortmund/DE), Marius Ueffing (Tübingen/DE) Andrea Urbani (Rome/IT), Uwe Völker (Greifswald/DE) Session Chairs & Pre-Congress Organizers The organizers of the Proteomic Forum 2015 wish to thank all those who have been actively involved in the organization of the scientific program as pre-conference program organizer or session chair. Their involvement is highly appreciated and has greatly contributed to the quality of the final program.

5

Welcome Note

Proteomic Forum 2015 

Dear Colleagues, On behalf of the Organizing Committee it is a great pleasure to invite you to join the Proteomic Forum 2015. Again, the Proteomic Forum will bring together leading experts from all over the world and provide a critical mass to present and to discuss progress and new technologies in all fields of proteome research and application. Join us to enjoy great science and Berlin, the exciting capital of Germany bridging Western and Eastern Europe. The congress will start with an Educational Workshop on Sunday, introducing newcomers to basic proteomic techniques and analytical strategies. Experts will benefit from being updated in the latest developments in proteomics technologies including data handling. Scientific sessions with a decent share of presentations selected from submitted abstracts will cover specific thematic areas in proteomics. Three poster sessions will provide the opportunity to present the latest results from your lab. Participants will have the opportunity to get insight into the most recent advances in proteomics technologies, Molecular and Cellular Proteomics as well as Clinical Proteomics and Systems Biology. Specifically, the program will cover New Technologies, Imaging, Subcellular Proteomics, Post-Translational Modifications, Gene Regulation and Epigenetics, Plant and Agricultural Proteomics, Microbial Proteomics, Disease Proteomics, Host-Pathogen Interaction & Antibiotical Resistance, Extracellular Vesicles, Structural Proteomics and Computational Omics. The congress will also focus on applications and commercialization in research, dia-gnostics and therapy. An industrial exhibition will present the latest products in Proteomics Technologies. You are cordially invited by the German Society of Proteome Research. We are delighted to welcoming you!

Albert Sickmann President of the DGPF and Congress Chair

Albert Sickmann

6

Organization and Imprint

Venue

Congress Chair

Technical University Berlin Strasse des 17. Juni 135 10623 Berlin (DE)

22–25 March 2015

Prof. Dr. Albert Sickmann Direktor Forschungsbereich Bioanalytik Leibniz–Institut für Analytische Wissenschaften – ISAS – e.V. Otto-Hahn-Strasse 6b 44227 Dortmund/DE

Congress Website

Congress Secretary


Dr. rer. nat. Christian Kleinhammer Life Science Service Burggasse 12/11 8010 Graz/AT

Date

Professional Congress Organizer Conventus Congressmanagement & Marketing GmbH Nadia Al-Hamadi/Sylvia Rudolph Carl-Pulfrich-Strasse 1 07745 Jena (DE) Phone +49 3641 31 16-315/-356 Fax +49 3641 31 16-241 [email protected] www.conventus.de Design/Layout Layout Pictures Print Print run Editorial Deadline

Martina Kogler, Koma Design, Berlin/DE The German Society of Proteome Research, Visit Berlin, Fotolia, Shutterstock 15 March 2015 600 14 March 2015

7

Index of Abstract IDs


Please find the explanation of abstract IDs in the following: ED

Abstracts of Educational Day Session

HUPO

Abstracts of HUPO Workshop

NC

Abstracts of News Corner Session

O

Abstracts of Oral Abstract Presentations

P

Abstracts of Poster Presentations

PL

Abstracts of Plenary Lectures

YIA

Abstracts of Young Investigator Award Session

All abstracts are provided within the abstract book that can be downloaded online at www.proteomic-forum.de.

Program Overview Sunday, 22 March 2015 R0104

R0107 09:00–10:30 EuPA Initiatives

R0110

R0112 09:00–10:30 HUPO Workshop Cardiovascular Disease

10:00–11:30 p. 13 Educational Day

11:00–12:30 EuPA Initiatives

p. 12

11:00–12:30 p. 14 HUPO Workshop Cardiovascular Disease 12:00-13:30 p. 14 Educational Day

p. 12

p. 15

14:00–15:45 EuPA Initiatives not open to public

14:00–15:30 Educational Day

14:00–15:30 HUPO Workshop Cardiovascular Disease

p. 15

p. 13

p. 14 16:00–17:30 Initiation of a Northwestern European Proteomics Working Group

16:00–17:30 News Corner Session

p. 15

p. 15

17:30–19:00 Welcome and Opening Lecture

p. 16 19:00–21:00 Poster Session A & Get Together Bioinformatics – Free Topics – Proteomics for Human Health I – Proteomics in Biotechnology – Quantitative Proteomics I

in Poster and Industrial Exhibition p. 16

Plenary Session

Symposium

News Corner, YIA Session

Lecture

Monday, 23 March 2015 R0104

R0110

R0107

09:00–10:30 New Technologies

p. 17

11:00–12:30 Post-Translational Modifcations and Pathways

p. 17 12:45–13:45 Thermo Fisher Scientific GmbH

12:45–13:45 Bruker Daltonik GmbH p. 17

14:00–15:00 Affinity Proteomics

14:00–15:00 Gene Regulation & Epigenetics p. 18

p. 18 14:00–15:00 Plant & Agricultural Proteomics I

p. 18

p. 19

15:00–16:30 Poster Session B Affinity Proteomics – Imaging – Mass Spectrometry Technologies – Plant and Microbial Proteomics I Post-Translational Modifcations I – Proteomics for Human Health II

Industrial Exhibition p. 19 16:30–17:30 Disease Proteomics

16:30–17:30 Imaging p. 19

16:30–17:30 Microbial Proteomics p. 20

p. 20

17:30–18:30 General Assembly DGPF

p. 20

Poster Session

Pre-Workshops

Lunch Symposium

Social Program

Meeting DGPF

Program Overview Tuesday, 24 March 2015 R0104 09:00–10:30 Interaction Proteomics

R0110

R0107

R0111

p. 21

11:00–12:30 Subcellular Proteomics

p. 21 12:45–13:45 12:45–13:45 Thermo Fisher Scientific GmbH Biognosys AG

12:45–13:45 Waters GmbH

p. 21 14:00–15:00 Host-Pathogen Interaction & Antibiotical Resistance

14:00–15:00 Plant & Agricultural Proteomics II

p. 22

p. 22

p. 22

14:00–15:00 Quantitative Strategies p. 23

p. 23

15:00–16:30 Poster Session C Plant and Microbial Proteomics II – Post-Translational Modifcations II – Proteomics for Human Health II Quantitative Proteomics II – Structural Proteomics – Subcellular Proteomics

in Poster and Industrial Exhibition p. 23 16:30–18:00 Young Investigator Award Session

Abstracts p. 24

from 20:00 Congress Dinner at the Restaurant “Nolle” p. 24

Plenary Session

Symposium

News Corner, YIA Session

Lecture

Wednesday, 25 March 2015 R0104

R0110

R0107

09:00–10:30 Systems Biology

p. 25

11:00–12:30 Proteomics in Health and Disease

p. 25 12:45–13:45 ABSCiex

12:45–13:45 Matrix Science Ltd. p. 25

14:00–15:00 Computational Omics I

14:00–15:00 Extracellular Vesicles p. 26

15:30–16:30 Computational Omics II

p. 25 14:00–15:00 Post-Translational Modifcations

p. 26

15:30–16:30 Career session

p. 27 16:30–17:45 Award Ceremony & Closing Lecture

p. 26

15:30–16:30 Structural Proteomics

p. 27

p. 27

p. 28

Poster Session

Pre-Workshops

Lunch Symposium

Social Program

Meeting DGPF

12

Scientific Program

Pre-Workshops • Sunday, 22 March 2015 09:00–10:30 HUPO Workshop • Cardiovascular Disease Mechanism and understanding complexity of protein modifications Room R0112 open for public Chair Merry Lindsey (Jacksonville/US) 09:00 Exploiting antibody and capture reagents for diseases affecting HUPO01 the cardiovasclular system Jochen Schwenk (Stockholm/SE) 09:25 Load induced changes and sex differences HUPO02 in the cardiac proteome Vera Regitz-Zagrosek (Berlin/DE) 09:50 Chamber specific protein expression in the human heart HUPO03 Anthony Gramolini (Toronto/CA) 10:15 Functional plasticity of cardiac interfibrillary mitochondria HUPO04 (IFM) as response mechanism to stress Juliana Heidler (Frankfurt a. M./DE) 10:30–11:00 Coffee break 11:00–12:30 HUPO Workshop • Cardiovascular Disease Biomarkers towards clinical relevance in CVD Room Chair 11:00 11:25

11:50

12:15

R0112 open for public Anthony Gramolini (Toronto/CA) Biomarkers to cardiac extracellular matrix HUPO05 Merry Lindsey (Jacksonville/US) High-throughput ELISA: sensitivity, specificity HUPO06 and understanding Oliver Pötz (Reutlingen/DE) Journey toward application: SRM multiplex assays, kits HUPO07 and high throughput applications Christoph Borchers (Victoria/CA) Comparative lipidomic and proteomic analysis of the plasma HUPO08 elimination rate of current lipid apheresis methods Stefan Wallner (Regensburg/DE)

12:30–14:00 Lunch break

13

Scientific Program


14:00–15:30 HUPO Workshop • Cardiovascular Disease In the road to translation in CVD Room Chair 14:00 14:25 14:50

15:15

R0112 open for public Jochen Schwenk (Stockholm/SE) Metabolomics profiling in late stage heart failure HUPO09 Peipei Ping (Los Angeles/US) Saving clinical trials: mechanistic proteomics for drug selection HUPO10 Jennifer van Eyk (Los Angeles/US) HUPO11 Moving from inventory to personalized medicine: reducing cardiovascular mortality Albert Sickmann (Dortmund/DE) Workshop summary: making it count, saving lives HUPO12 by reducing heart disease John Yates (San Diego/US)

09:00–10:30 Workshop • EuPA Initiatives Session I Room R0107 09:00 Welcome and introduction to EuPA initiatives 2015–2017 Andrea Urbani (Rome/IT) 09:30 Past and future perspectives of EuPA Emøke Bendixen (Aarhus/DK) 09:45 EuPA educational program Karl Mechtler (Vienna/AT) 10:00 EuPA congress and communication activities Fernando Corrales (Pamplona/ES) 10:15 EuPA journals initiatives Andrew Pitt (Birmingham/UK)

open for public

14

Scientific Program

Sunday, 22 March 2015 10:30–11:00 Coffee break 11:00–12:30 Workshop • EuPA Initiatives Session II Room R0107 11:00 EuPA funding initiatives Albert Sickmann (Dortmund/DE) 11:15 EuPA Initiatives Andrey Lisitsa (Moscow/RU) 11:30 iMOP 11:45 Imaging 12:00 Standardization 12:15 Biobanking 12:30 Proteome Informatics 12:45 Large Consortia Integration

open for public

13:00–14:00 Lunch break 14:00–15:00 Workshop • EuPA Initiatives Internal EuPA EC/Advisory Board Meeting Coffee with the “Senators” Room R0107 not open to public Chairs Friedrich Lottspeich (Stockdorf/DE), Peter Roepstorff (Odense/DK) 15:00–15:45 Workshop • EuPA Initiatives EuPA Company Club Meeting Room R0107 Meet the proteomics technology vendors and companies 10:00–11:30 Workshop • Educational Day Session I Room R0110 Chair Sara Galozzi (Bochum/DE) 10:00 Protein quantification by SWATH Christina Ludwig (Zurich/CH) 10:45 Label-free quantification using ion-mobility enhanced data-independent acquisition (UDMSE) Stefan Tenzer (Mainz/DE) 11:30–12:00 Coffee break

not open to public

open for public ED1 ED2

15

Scientific Program


12:00–13:30 Workshop • Educational Day Session II Room R0110 Chair Carina Ramallo Guevara (Bochum/DE) 12:00 Quantification of PTMs Martin Røssel Larsen (Odense/DK) 12:45 Targeted quantification by SRM and PRM Bruno Domon (Strassen/LU)

ED3 ED4

13:30–14:00 Lunch break 14:00–15:30 Workshop • Educational Day Session III Room R0110 Chair Christine von Törne (Neuherberg/DE) 14:00 The nuts and bolts of performing top down MS and top down proteomics Neil Kelleher (Evanston/US) 14:45 Bioinformatic challenges in quantitative proteomics Oliver Kohlbacher (Tübingen/DE)

ED5

ED6

15:30–16:00 Coffee break 16:00–17:30 Workshop • Initiation of a Northwestern European Proteomics Working Group Room R0110 Chair Bruno Domon (Strassen/LU) 16:00–17:30 Room Chair 16:00 16:10

16:20

16:30

News Corner R0112 Helmut E. Meyer (Bochum/DE) A universal method for peptide identification on orbitrap fusion MS Pamela Saliba (Dreieich/DE) Systematic development of targeted proteomics assays using expressed prostatic secretions: liquid biopsies to identify extracapsular prostate cancers Thomas Kislinger (Toronto/CA) Biophysical analysis of molecular interactions with electro-switchable DNA nanolevers on a chip Ulrich Rant (Planegg/DE) Individual pharmacokinetics of thiol-containing drugs can be estimated by their interaction with the serum protein transthyretin Andrea Henze (Nuthetal/DE)

NC1 NC2

NC3

NC4

16

16:40

16:50

17:00

17:10 17:20

Scientific Program

A novel 6x5 peptide mixture for LC-MS/MS performance monitoring Mike Rosenblatt (Madison/US) Application of oncoproteomics in changing the treatment paradigm in acute lymphoblastic leukemia (ALL) Elena Lopez Villar (Madrid/ES) Capillary LC-MSMS on tripleTOF® mass spectrometry systems for proteomic applications Nicholas Morrice (Warrington/UK) Early warning signals of aging in the hematopoietic stem cell niche Marco Hennrich (Heidelberg/DE) Multiplexed mass spectrometric analysis of proteostasis elucidates the mode-of-action of cancer drugs Marcus Bantscheff (Heidelberg/DE)

17:30–19:00 Welcome & Opening Lecture Room R0104 Chair Albert Sickmann (Dortmund/DE), Bruno Domon (Strassen/LU), Andrea Urbani (Rome/IT) 18:00 Understanding diseases using mass spectrometry John Yates (San Diego/US) 19:00–21:00 Poster Session A* & Get-Together Room Foyers ground and first floor, Atrium Bioinformatics (see p. 29 ff.) Free Topics (see p. 30 ff.) Proteomics for Human Health I (see p. 32 ff.) Proteomics in Biotechnology (see p. 34 ff.) Quantitative Proteomics I (see p. 35 ff.) *All posters are displayed throughout the whole congress.

NC5

NC6

NC7

NC8 NC9

PL01

P014–P030 P032–P049 P123–P145 P192–P198 P199–P215

17

Scientific Program


Monday, 23 March 2015 09:00–10:30 Room Chairs 09:00 09:30 10:00

Plenary Symposium • New Technologies R0104 Oliver Pötz (Reutlingen/DE), Christian Huber (Salzburg/AT) Capillary electrophoresis for bottom-up proteomics Norman J. Dovichi (Notre Dame/US) On the value of proteoform resolved measurements Neil Kelleher (Evanston/US) Advanced time-domain data analysis for improved FTMS-based proteomics Yuri Tsybin (Lausanne/CH)

PL02 PL03 PL04

10:30–11:00 Industrial exhibition and coffee break 11.00–12.30 Room Chairs 11:00

11:30 12:00

Plenary Symposium • Post-Translational Modifications and Pathways R0104 Albert Sickmann (Dortmund/DE), Sasha Singh (Boston/US) Computational analysis of mitochondrial protein dynamics PL05 in heart failure Peipei Ping (Los Angeles/US) Comprehensive strategy for the study of PTMs PL06 Martin Røssel Larsen (Odense/DK) Exploring alternative proteases and fragmentation methods PL07 for proteomics Albert J. R. Heck (Utrecht/NL)

12:30–14:00 Industrial exhibition and lunch break 12:45–13:45 Lunch Symposium – Thermo Fisher Scientific GmbH Of proteins, peptides and others big and small with orbitraps big and small Room R0110 This workshop will describe recent innovation in the analysis of both intact and digested proteins and other molecules on different Orbitrap mass spectrometer platforms. Shannon Eliuk will describe the sophisticated workflows possible on the Thermo Scientific™ Orbitrap Fusion™ Tribrid™ Mass Spectrometer enabling discovery in the fields of proteomics, glycomics and glycoproteomics. Prof Albert Heck will discuss his experience in the field of analysis of intact proteins and proteins complexes as well as his recent authoratitive work in the area of the proteomics of cellular reprogramming. 12:45 From proteins to omes: supreme versatility for qualitative and quantitative analysis Shannon Eliuk (Dreieich/DE)

18

13:05 13:25

Scientific Program

Proteomics of cellular reprogramming Albert J. R. Heck (Utrecht/NL) Exploring the benefits of unrestrained mass range and high resolving power on an orbitrap (Jumbo) for top-down and native analysis of proteins and protein complexes Albert J. R. Heck (Utrecht/NL)

12:45–13:45 Lunch Symposium – Bruker Daltonik GmbH Orthogonal time-of-flight based proteomics Room 12:45

13:15

14:00–15:00 Room Chair 14:00

14:20

14:40

14:00–15:00 Room Chairs 14:00

14:20

14:40

R0107 Accurate proteome quantification and bioinformatics analysis of impact II data with MaxQuant Jürgen Cox (Munich/DE) Evaluation of quadrupole time-of-flight mass spectrometry for qualitative and quantitative proteomic Stephanie Kaspar (Bremen/DE) Symposium • Affinity Proteomics R0104 Matthias Mann (Martinsried/DE) Quantitative chemical proteomics identifies the interactome of PDE3A in cancer cells Eleonora Corradini (Utrecht/NL) Deciphering preferential interactions within supramolecular protein complexes: the proteasome case Marie-Pierre Bousquet-Dubouch (Toulouse/FR) Unravelling global RNA-binding protein architectures by quantitative proteomics Christian Frese (Heidelberg/DE) Symposium • Gene Regulation & Epigenetics R0110 Brian T. Chait (New York/US), Kathryn Lilley (Cambridge/UK) Characterization of histone PTM crosstalk by middle-down mass spectrometry and data integration Veit Schwämmle (Odense/DK) Chromatin factors mapped by co-regulation patterns in proteomic experiments Georg Kustatscher (Edinburgh/UK) Novel approaches to investigate global and locus-specific chromatin composition Jeroen Krijgsveld (Heidelberg/DE)

O01

O02

PL10

O03

O04

O05

19

Scientific Program


14:00–15:00 Room Chair 14:00 14:20 14:40

Symposium • Plant & Agricultural Proteomics I R0107 Hans-Peter Braun (Hannover/DE) Mining protein modifications in arabidopsis proteomics data Joshua Heazlewood (Berkeley/US) Understanding plant biology through functional proteomics Sacha Baginski (Halle/DE) Geolocalization of chloroplast proteins: an important feature for a better understanding of chloroplast metabolism Myriam Ferro (Grenoble/FR)

15:00–16:30 Poster Session B* and coffee break Room Foyers ground, first floor, Atrium Affinity Proteomics (see p. 38 ff.) Imaging (see p. 39 ff.) Mass Spectrometry Technologies (see p. 40 ff.) Plant and Microbial Proteomics I (see p. 41 ff.) Post-Translational Modifcations I (see p. 42 ff.) Proteomics for Human Health II (see p. 44 ff.) 16:30–17:30 Room Chair 16:30

16:50

17:10

Symposium • Disease Proteomics R0104 Andrea Urbani (Rome/IT) The oncogenic EGFR network hairball: proteomic approach for finding the tangled knot Mohamed Ali Jarboui (Tübingen/DE) Altered phosphorylation and Calpain-dependent cleavage profiles of Scott syndrome platelets revealed by quantitative proteomics Fiorella Andrea Solari (Dortmund/DE) A renal Fanconi-syndrome caused by mistargeting of a peroxisomal protein Joerg Reinders (Regensburg/DE)

*All posters are displayed throughout the whole congress.

PL08 PL09 O06

P001–P013 P050–P055 P056–P065 P066–P079 P092–P107 P146–P169

O07

O08

O09

20

16:30–17:30 Room Chair 16:30 16:50

17:10 16:30–17:30 Room Chair 16:30

16:50

17:10

Scientific Program

Symposium • Imaging R0110 Gerd Schmitz (Regensburg/DE) Peptide MALDI Imaging – searching for the best protocol Corinna Henkel (Dortmund/DE) MALDI mass spectrometry imaging revealed specific drug distribution of vemurafenib against BRAF V600E in malignant melanoma tissues Yutaka Sugihara (Lund/SE) Single cell proteomics in health and disease Bernd Bodenmiller (Zurich/CH) Symposium • Microbial Proteomics R0107 Uwe Völker (Greifswald/DE) Metaproteomic analysis of taxonomic and functional signatures in several grana padano experimental caseification models Alessio Soggiu (Milan/IT) Miscincorporation of nonproteinogenic amino acids in bacterial proteome analyzed by global, unbiased quantitative MS of protein modifications Boris Macek (Tübingen/DE) Metaproteomics – novel insights into old questions in medical microbiology and microbial ecology Kathrin Riedel (Greifswald/DE)

17:30–18:30 General Assembly German Society of Proteome Research (DGPF e.V.) Room R0110 1. Report of the Board 1.1 General Development of the DGPF 1.2. Conferences, Education 1.3. Working Groups and projects 1.4. Report of the Treasurer 2. Report of the Account Reviewers 3. Discharge of the Board 4. Change of Statutes 5. Regular election of the Board and the Extended Board 6. Election of Account Reviewers 7. Additional Points requested by members 8. Date of the 15th General Assembly 9. Miscellaneous

O10 O11

PL11

O12

PL12

PL13

21

Scientific Program


Tuesday, 24 March 2015 09:00–10:30 Room Chairs 09:00

09:30 10:00

Plenary Symposium • Interaction Proteomics R0104 Julia Burkhart (Dortmund/DE), Friedrich Lottspeich (Stockdorf/DE) Chemical cross-linking and mass spectrometry for mapping protein-protein interactions Andrea Sinz (Halle, Saale/DE) Protein-lipid networks Anne-Claude Gavin (Heidelberg/DE) The proteome of the synapse Henning Urlaub (Göttingen/DE)

PL14

PL15 PL16

10:30–11:00 Industrial exhibition and coffee break 11:00–12:30 Room Chairs 11:00

11:30

12:00

Plenary Symposium • Subcellular Proteomics R0104 Anne-Claude Gavin (Heidelberg/DE), Steven Verhelst (Munich/DE) Protein turnover by progressive 15N labelling for analysis of plant mitochondrial biogenesis and assembly Harvey Millar (Crawley/AU) Hybrid methods for defining the structure and function of cellular machines Brian T. Chait (New York/US) Subcellular protein localization by mass spectrometry Kathryn Lilley (Cambridge/UK)

PL17

PL18

PL19

12:30–14:00 Industrial exhibition and lunch break 12:45–13:45 Lunch Symposium – Thermo Fisher Scientific GmbH High throughput, optimized shotgun proteomics with DDA and DIA on the Thermo Scientific™ Q exactive™ Room R0110 For most of the short history of proteomics most mass spectrometry based workflows have worked via data dependent LCMS experiments. However, in the last several years there has been growing interest in workflows absent of data dependent mass spectral acquisition. These approaches have come to be known as data independent analysis (DIA). In addition, a great deal of development has occurred up-front of the mass spectrometer to optimize and simplify proteomics workflows. In this workshop two speakers, Prof. Matthias Mann and Dr. Yue Xuan, will discuss recent developments in optimizing shotgun proteomics experiments. Prof. Mann will focus on achievements up-front of the mass spectrometer aimed at maximizing up-time and throughput while Dr. Xuan will focus on the data acquisition approach.

22

12:45

13:15

Scientific Program

DIA alysis on the orbitrap platform – using novel data independent analysis methods to obtain characterization of a biological pathway Yue Xuan (Dreieich/DE) Technology development towards high-throughput proteomics Matthias Mann (Martinsried/DE)

12:45–13:45 Lunch Symposium – Biognosys AG Next-generation proteomics technologies for comprehensive proteome quantification Room R0107 12:45 Introduction Oliver Rinner (Zurich/CH) 12:50 Data independent acquisition (DIA) using orbitrap analyzers Lukas Reiter (Zurich/CH) 13:15 Evolution and regulation of metabolism by systematic SWATH-MS profiling Markus Ralser (Cambridge/UK) 12:45–13:45 Lunch Symposium – Waters GmbH Travelling wave ion mobility spectrometry (TWIMS) enhanced MS technologies for OMICS sciences Room R0111 12:45 Introduction Marc Kipping (Eschborn/DE) 12:50 Ion mobility – mass spectrometry of glycans and glycopeptides Kevin Pagel (Berlin/DE) 13:10 Perfect timing – fragment ion mobility based performance increase on a qTOF Dominic Helm (Munich/DE) 13:30 Characterization of the post-synaptic density proteome using label-free quantitative proteomics Stefan Tenzer (Mainz/DE) 14:00–15:00 Room Chair 14:00 14:20

14:40

Symposium • Host-Pathogen Interaction & Antibiotical Resistance R0104 Paola Roncada (Milan/IT) AP-MS and BioID analysis of the HBx Viral-Host interactome Emanuela Milani (Zurich/CH) Physiology of staphylococcus aureus isolated from human macrophages and the role of CodY in host pathogen interaction Susanne Engelmann (Braunschweig/DE) Host pathogen interactions in the pig, and the relevance to antimicrobial resistance Emøke Bendixen (Aarhus/DK)

O13 O14

PL21

23

Scientific Program


14:00–15:00 Room Chair 14:00

14:20

14:40

14:00–15:00 Room Chair 14:00

14:20

14:40

Symposium • Plant & Agricultural Proteomics II R0110 Harvey Millar (Crawley/AU) Evaluation of plant genetic resources for stress defence mechanisms using proteomic approaches Hans-Peter Mock (Seeland/DE) Quantitative proteomic analysis of lysine acetylation in arabidopsis leaves Iris Finkemeier (Cologne/DE) Mass spectrometry identification of free flow separated protein complexes from the thylakoid membrane of arabidopsis thaliana Lutz Eichacker (Stavanger/NO) Symposium • Quantitative Strategies R0107 Roland Kellner (Darmstadt/DE) Quantitative proteomics meets highly sensitive phosphoproteomics – a simple workflow that combines both for low sample amount experiments Stefan Loroch (Dortmund/DE) Extensive proteome remodeling and protein aggregation in C. elegans revealed by SILAC-based quantitative proteomics Dirk Walther (Martinsried/DE) HR-PRM for quantification of low abundance plasma proteins implicated in cardiovascular diseases Sasha Singh (Boston/US)

15:00–16:30 Poster Session C* and coffee break Room Foyers ground, first floor, Atrium Plant and Microbial Proteomics II (see p. 47 ff.) Post-Translational Modifcations II (see p. 48 ff.) Proteomics for Human Health III (see p. 49 ff.) Quantitative Proteomics II (see p. 52 ff.) Structural Proteomics (see p. 53 ff.) Subcellular Proteomics (see p. 54 ff.) *All posters are displayed throughout the whole congress.

PL20

O15

O16

O17

O18

PL22

P080–P091 P108–P122 P170–P190 P216–P232 P233–P238 P239–P251

24

16:30–18:00 Room Chair 16:30 16:45

17:00

17:15

17:30 17:45

from 20:00

Scientific Program

Young Investigator Session (see abstracts on p. 57 ff.) R0104 Roland Kellner (Darmstadt/DE) GRP78 phosphorylation dynamics during sperm maturation Vivian Lobo (Mumbai/IN) Exposure of mycobacterium tuberculosis to thioridazine alters the cell envelope permeability; an alternative mechanism for the synergistic effects with other antibiotics Jeroen de Keijzer (Leiden/NL) Improving proteotypic peptide prediction using information retrieval based measures Andrea Argentini (Ghent/BE) Label-free quantification of GeLC-MALDI data with a novel software reveals a subtype-specific protein signature for pancreatic ductal adenocarcinoma Wiebke Nadler (Heidelberg/DE) Proteomic analysis of barley cell nuclei Ivo Chamrád (Olomouc/CZ) Proteomic evaluation of nucleocytoplasmic partitioning and the CRM1-dependent nuclear exportome Samir Karaca (Göttingen/DE) Congress Dinner in the Restaurant “Nolle” (see p. 71)

YIA1 YIA2

YIA3

YIA4

YIA5 YIA6

25

Scientific Program


Wednesday, 25 March 2015 09:00–10:30 Room Chairs 09:00 09:30 10:00

Plenary Symposium • Systems Biology R0104 Marius Ueffing (Tübingen/DE), René P. Zahedi (Dortmund/DE) Genetic variability and the quantitative proteome Ruedi Aebersold (Zurich/CH) Time resolved insulin signaling in vivian Matthias Mann (Martinsried/DE) Protein abundance evolution and its systems-level constraints Christian von Mering (Zurich/CH)

PL23 PL24 PL25

10:30–11:00 Industrial exhibition and coffee break 11:00–12:30 Room Chairs 11:00 11:30 12:00

Plenary Symposium • Proteomics in Health and Disease R0104 Stefanie Hauck (Munich/DE), Helmut E. Meyer (Bochum/DE) Proteomic approach towards personalized sarcoma treatment Tadashi Kondo (Tokyo/JP) N-terminomics in immunity: mechanisms of disease Christopher M. Overall (Vancouver/CA) MRM and iMALDI for clinical research and diagnostic Christoph Borchers (Victoria/CA)

12:30–14:00 Industrial exhibition and lunch break 12:45–13:45 Lunch Symposium – ABSciex Germany GmbH NEWS from SCIEX Room R0110 12:45 Analysis and quantification of complex posttranslational modifications with LC and capillar electrohoresis MS/MS Axel Imhof (Munich/DE) 13:15 The next step of SWATH™ in next generation proteomics and where it meets next generation sequencing Jörg Dojahn (Darmstadt/DE) 12:45–13:45 Lunch Symposium – Matrix Science Ltd. Large Scale Proteomics Room R0107 12:45 Analysis of the human proteome in proteomicsDB Bernhard Kuster (Munich/DE) 13:15 Reporting complex quantitation data sets using mascot insight Patrick Emery (London/UK)

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Symposium • Computational Omics I R0104 Lennart Martens (Ghent/BE) Proteogenomic RNA-Seq data integration beyond six-frames and single nucleotide variations Bernhard Y. Renard (Berlin/DE) Summarization vs. peptide-based models in label-free quantitative proteomics: performance, pitfalls and data analysis guidelines Lieven Clement (Ghent/BE) Applications of predictions of peptide properties Lukas Käll (Solna/SE) Symposium • Extracellular Vesicles R0110 Corinna Henkel (Dortmund/DE) Analysis of extracellular vesicles (EVs) in diagnostics and quality control of blood products in transfusion medicine Gerd Schmitz (Regensburg/DE) Development of a blood test for Alzheimer’s disease by proteomic identification a specific phenotype in platelets Maria Zellner (Vienna/AT) Role of extracellular vesicles in neurological diseases associated with protein misfolding Andrew Hill (Parkville/AU) Symposium • Post-Translational Modifcations R0107 Martin Røssel Larsen (Odense/DK) Chemical proteomics highlights protein kinases involved in macrophage differentiation and function Erik Richter (Greifswald/DE) Identifying kinase substrates via a heavy ATP kinase assay and quantitative mass spectrometry (HAKA-MS) Andre Müller (Vienna/AT) Hydrophilic interaction chromatography for the enrichment of sialylated glycopeptides Lylia Drici (Odense/DK)

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Symposium • Computational Omics II R0104 Lukas Käll (Solna/SE) Speaking to spectra: what we can learn without using search engines Lennart Martens (Gent/BE) MS/MS-free protein identification in complex mixtures using multiple enzymes with complementary specificity Mark Ivanov (Mikhail/RU) Advances in pulse-chase experiments using mass spectrometry revealing protein turnover rates Christian Fufezan (Münster/DE)

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15:30–16:30 Career Day Room R0110 Chairs Kai Stühler (Düsseldorf/DE), Christian Moritz (Kaiserslautern/DE) Well-known companies present their current job offers and inform about requested competences for candidates, their average job postings during a year, the success of unsolicited applications and answer to many more questions of interested participants and potential job candidates. The following companies will present during the symposium:

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Symposium • Structural Proteomics R0107 Christoph Borchers (Victoria/CA) Using cross-linking coupled to mass spectrometry and integrated modeling to study the molecular architecture of the 40S•eIF1•eIF3 translation initiation complex Florian Stengel (Zurich/CH) Scalable HX-MS methods to characterize ultra-large protein complexes David Schriemer (Calgary/CA) Crosslinking-MS analysis combined with molecular modelling provides novel insight into the architecture of the Parkinson’s-associated protein kinase LRRK2 Giambattista Guaitoli (Neuherberg/DE)

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16:30–16:45 Award Ceremony Room R0104 Award Ceremony of Young Investigator Prizes, Promotion Prize and Poster Prizes

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Closing Lecture R0104 Marius Ueffing (Tübingen/DE) Metabolic profiling technologies and applications Elaine Holmes (London/UK) Farewell

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Poster Sessions


Poster Session A • Sunday, 22 March 2015, 19:00–20:30 Bioinformatics Free Topics Proteomics for Human Health I Proteomics in Biotechnology Quantitative Proteomics I

P014–P031 (see p. 29 ff.) P032–P049 (see p. 30 ff.) P123–P145 (see p. 32 ff.) P192–P198 (see p. 34 ff.) P199–P215 (see p. 35 ff.)

Foyer ground floor Foyer ground floor Atrium Atrium Atrium

Bioinformatics P014 Robust peptide-based models in quantitative proteomics L. Goeminne, K. Gevaert, K. Vandepoele, L. Clement (Gent/BE) P015 Analysis of labeled and non-labeled DIA and DDA proteomic data using Progenesis QI for proteomics L. A. Gethings, G. Atwal, M. Palmer, M. Kipping (Wilmslow/UK), J. Pope (Newcastle upon Tyne/UK), A. Collins, A. Jones (Liverpool/UK), J. I. Langridge, J. P. Vissers (Wilmslow/UK) P016 PRIDE Cluster: how to take advantage of spectrum clustering in a heterogeneous repository of MS proteomics data R. Wang (Cambridge/UK), J. Griss (Cambridge, Vienna/US, AT), S. Lewis (Cambridge/US), J. A. Dianes, N. D. Toro, H. Hermjakob, J. A. Vizcaino (Cambridge/UK) P017 Selection of SRM-compatible peptides to detect single amino acid variations O. Kiseleva, A. Lisitsa, G. Krasnov, A. Kudryavtseva (Moscow/RU) P018 Estimating the limits of detection in metaproteomics M. Kuhring, B. Y. Renard (Berlin/DE) P019 Using public proteomics data to find the blind spots in mass spectrometry K. Verheggen (Gent/BE) P020 – withdrawn P021 – withdrawn P022 Peakjuggler – A proteome discoverer node for label-free MS1 quantification J. Doblmann, F. Dusberger, K. Mechtler (Vienna/AT) P023 GelMap: annotation and evaluation of gel-based proteomic data J. Klodmann, M. Senkler, H.-P. Braun (Hannover/DE) P024 Combining SWATH/DIA proteomics data with large scale RNA expression data enables cross-omics quantitative biology O. Rinner, C. Escher, R. Bruderer, O. Bernhardt, S. Messner (Schlieren/CH), S. Bleuler, P. Zimmermann (Zurich/CH), L. Reiter (Schlieren/CH)

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P025 MetaProteomeAnalyzer – a software suite for the functional and taxonomic characterization of (meta) proteome data T. Muth, A. Behne, R. Heyer, F. Kohrs, M. Hoffmann, U. Reichl (Magdeburg/DE), L. Martens (Gent/BE), E. Rapp (Magdeburg/DE) P026 Informatics support for isomeric separation and the structural identification of labeled N-glycans from proteins M. Oppermann (Stockholm/SE) P027 On the use of multiple databases for DB search in mass spectrometry-based proteomics C. Has, M. D. Munga, C. Ciftci, J. Allmer (Urla Izmir/TR) P028 A search strategy for the identification of cross-linked peptides based on their CID behavior S. Giese (Berlin, Edinburgh/DE, UK), L. Fischer (Edinburgh/UK), J. Rappsilber (Berlin, Edinburgh/DE, UK) P029 xiFDR – false discovery rate estimation in cross-linking/mass spectrometry L. Fischer (Edinburgh/UK), J. Rappsilber (Berlin, Edinburgh/DE, UK) P030 Complexation of proteins with cationic porphyrins and significance of fatty acids A. Gyulkhandanyan (Yerevan/AM) P031 Speaking to spectra: what we can learn without using search engines L. Martens (Gent/BE) Free Topics P032 Computational and mass spectrometry-based workflow for the discovery and validation of human missing proteins: application to chromosomes 2 and 14 Y. Vandenbrouck (Grenoble/FR), C. Carapito (Strasbourg/FR), L. Lane (Geneva/CH), M. Benama (Grenoble/FR), A. Opsomer (Strasbourg/FR), E. Mouton-Barbosa, L. Garrigues, A. Gonzalez de Peredo (Toulouse/FR), A. Burel (Strasbourg/FR), C. Bruley (Grenoble/FR), A. Gateau (Geneva/FR), D. Bouyssie (Toulouse/FR), M. Jaquinod (Grenoble/FR), O. Burlet-Schiltz (Toulouse/FR), A. Van Dorsselaer (Strasbourg/FR), J. Garin (Grenoble/FR) P033 Odorant binding proteins in the field mouse (Apodemus sylvaticus) Z. Zdráhal (Brno/CZ), L. Havrdova (Prague/CZ), O. Sedo (Brno/CZ), P. Klempt, D. Vinkler, R. Stopkova, P. Stopka (Prague/CZ) P034 How and when, came into existence, the first stem cells on Earth? R. Amirmardfar (Tabriz/IR)

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P035 Comparative metabolomics analysis of prostate cancer cells with different ethnic backgrounds J. Nyalwidhe, T. Burch (Norfolk/US), J. Rhim (Bethesda/US), J. I. Langridge, A. Baker, G. Isaac (Wilmslow/UK) P036 Influence of column- and gradient-length on protein identification by nanoLC-MS/MS of whole cell shotgun proteome analysis of marine bacteria L. Wöhlbrand, R. Rabus, B. Blasius, C. Feenders (Oldenburg/DE) P037 MALDI-TOF MS profiling of spider venoms O. Bocanek, O. Sedo, S. Pekar, Z. Zdráhal (Brno/CZ) P038 Low temperature stress has an impact on the regulation of sexual development in Aspergillus nidulans B. Hanf, T. Krüger, D. Mattern, A. A. Brakhage, O. Kniemeyer (Jena/DE) P039 Exploring the potential of a last generation UHR-Q-TOF for rapid generation of accurate information on proteoforms mass, distribution and relative abundancy P.-O. Schmit (Wissembourg/FR), W. Jabs, K. Meyer, C. Albers, S. Kaspar (Bremen/DE) P040 Pinpointing protein-protein interactions of the yeast mediator complex by glutaraldehyde cross-linking and GeLCMS M. Bach, H. Uthe, J. T. Vanselow, A. Schlosser (Würzburg/DE) P041 LysargiNase mirrors trypsin for protein C-termini and methylation site identification P. F. Huesgen (Jülich, Vancouver/DE, CA), P. F. Lange, L. D. Rogers, N. Solis, U. Eckhard (Vancouver/CA), O. Kleifeld (Clayton/AU), T. Goulas, F. X. Gomis-Ruth (Barcelona/ES), C. M. Overall (Vancouver/CA) P042 Optimization of capillary electrophoresis method for monitoring of RepA protein – DNA binding reaction P. Baran, P. Zaleski, P. Wawrzyniak, A. Bierczynska-Krzysik, G. Plucienniczak, A. Plucienniczak (Warszawa/PL) P043 Comparative venomic characterization of four medicinally important turkish vipers (Macrovipera lebetina, Montivipera xanthina, Vipera ammodytes, V. kaznakovi) N. Igci (Ankara/TR), D. Petras (Berlin/DE), B. Gocmen (Izmir/TR), R. Süssmuth (Berlin/DE), M. Z. Yildiz (Adiyaman/TR), F. D. Ozel-Demiralp (Ankara/TR) P044 Metaproteome analysis of the salivary and tongue proteome A. Rabe, M. Gesell Salazar, S. Fuchs, H. Senkbeil, T. Kocher, U. Völker (Greifswald/DE)

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P045 Protein expression patterns in ileal tissue of sheep infected with Mycobacterium avium subsp. paratuberculosis: in search for disease mechanisms and biomarkers S. Pisanu (Alghero/IT), T. Cubeddu, S. Rocca (Sassari/IT), S. Uzzau, M. F. Addis (Alghero/IT) P046 Novel findings on innate immunity of the mammary gland and their implications for diagnosis, as inferred from the sheep mastitis model S. Pisanu, T. Cubeddu (Sassari/IT), S. Ghisaura (Alghero/IT), D. Pagnozzi, G. Marogna, S. Rocca (Sassari/IT), S. Uzzau, M. F. Addis (Alghero/IT) P047 Differential peptidomics of raw and pasteurized ovine milk cheese S. Pisanu (Alghero/IT), M. Pes (Olmedo/IT), R. Anedda (Alghero/IT), D. Pagnozzi (Alghero/IT), A. Pirisi (Olmedo/IT), S. Uzzau, M. F. Addis (Alghero/IT) P048 Omics analysis in the brain: how similar are transcriptome and proteome? C. Moritz, T. Mühlhaus, T. Schulenborg (Kaiserslautern/DE), S. Tenzer (Mainz/DE), E. Friauf (Kaiserslautern/DE) P049 In vivo proteomics approaches unravel adaptation strategies of the pathogen S. aureus HG001 and host defense mechanisms of human alveolar epithelial cells upon internalization K. Surmann, M. Simon, P. Hildebrandt, S. Michalik, H. Pförtner, S. Stentzel, V. M. Dhople, R. Schlüter, P. Gierok, M. Lalk, B. Bröker, F. Schmidt, U. Völker (Greifswald/DE) Proteomics for Human Health I P123 Concentration gradients in human hippocampal regions of interest (CA1, CA2, CA3, fascia dentata) – relevance for neurodegenerative diseases? A. Schrötter (Dortmund/DE), H. Heinsen (Würzburg/DE), L. T. Grinberg (San Francisco, Sao Paulo/US, BR), H. E. Meyer (Dortmund/DE) P124 Proteome-wide analysis reveals an age-associated cellular phenotype of in situ aged human fibroblasts D. M. Waldera-Lupa, F. Kalfalah (Düsseldorf/DE), S. Sass (Neuherberg/DE), F. Kruse (Düsseldorf/DE), V. Rieder, J. Tigges, F. Boege (Düsseldorf/DE), F. Theis (Garching/DE), G. Reifenberger, K. Stühler (Düsseldorf/DE) P125 Beyond the hairball, uncovering the quantitative EGFR interactome in oncogenic K-Ras Colorectal cancer model L. Dernayka, M. A. Jarboui (Tübingen/DE), C. Raso, S. Kennedy (Dublin/IE), K. Bryan (Adelaide/AU), K. Boldt, M. Ueffing (Tübingen/DE) P126 Protein alterations in women with chronic widespread pain – a proteomic study of the trapezius muscle P. Olausson, D. Sjöström, E. Blixt, N. Ghafouri, B. Ghafouri, B. Gerdle (Linköping/SE)

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P127 Investigating biological variation in human hepatocytes of phase I and II drug metabolism enzymes N. Morrice (Warrington/UK), C. Baumann, J. Dojahn, M. Macht (Darmstadt/DE), C. Hunter (Redwood City/US), H. Zhang (Groton/US), X. Wang (Framingham/US) P128 Comparative proteomics analysis reveals that small GTPase signaling pathway may playimportant roles in cadmium induced colon cell maligant transformation J. Lu (Zhenjiang/CN) P129 Lipidomic and proteomic characterization of platelet derived extracellular vesicles F. El Magraoui (Dortmund/DE), A. Pienimaeki Roemer (Regensburg/DE), K. Kuhlmann (Bochum/DE), G. Schmitz (Regensburg/DE), H. E. Meyer (Dortmund/DE) P130 MALDI imaging for determining the protein network associated with atrial fibrillation H. Thiele, S. Mohamed, H. H. Sievers, J. Yan, T. Hanke (Lübeck/DE), O. Klein (Berlin/DE) P131 Dynamic analysis of differential global phosphorylation changes in lung cancer by discovery proteomics D. Treue, J. Lechner, M. Bockmayr (Berlin/DE), B. Thomas (Oxford/UK), F. Klauschen (Berlin/DE) P132 A mass spectrometry method to identify naturally processed and presented HPV epitopes R. Blatnik, S. Hoppe, M. Wühl, C. Rösli, A. B. Riemer (Heidelberg/DE) P133 Identification of proteins and molecular pathways regulating tumor-initiating pancreatic cancer stem cells employing proteomics and transcriptomics L. Weilnböck, A. Loipetzberger (Salzburg/AT), M. Rurik (Tübingen/DE), O. Kohlbacher (Tübingen/DE), F. Aberger, C. Huber (Salzburg/AT) P134 Integrated OMICS reveal organ-specific proteome deterioration during aging in rat A. Ori (Heidelberg/DE), B. Toyama (La Jolla/US), M. Harris (Berkeley/US), T. Bock (Heidelberg/DE), N. Ingolia (Berkeley/US), M. Hetzer (La Jolla/US), M. Beck (Heidelberg/DE) P135 Deorphanizing the matrix metalloprotease MT5-MMP via proteomic substrate identification in the secretome J. Herber, S. Lichtenthaler (Munich/DE) P136 Ribosomal proteins generate protein signatures in health & disease A. Friedrich, C. Brandl, T. Karl, B. Wimmer, P. Radler, J. Teufl, A. Klausegger (Salzburg/AT), S. Klauck (Heidelberg/DE), M. Breitenbach, H. Hintner, J. W. Bauer, H. Breitenbach-Koller (Salzburg/AT)

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P137 Investigations into the mechanism of action of the neurotrophic drug Cerebrolysin F. Füssl (Salzburg/AT), S. Winter (Unterach/AT), C. Huber (Salzburg/AT) P138 Effect of ascitic fluid from ovarian cancer patients on the proteome of transformed cells A. Toledo-Leyva, J. C. Villegas-Pineda, J. C. Osorio-Trujillo, V. Hernández-Ramírez, D. Gallardo-Rincón, P. Talamás-Rohana (Mexico/MX) P139 TXNL1 characterize aneuploid subtypes of colorectal and endometrial cancer T. Gemoll, U. J. Roblick, S. Szymczak (Lübeck/DE), T. Braunschweig (Aachen/DE), S. Becker (Stockholm/SE), U. Hellman (Uppsala/SE), C. Lundgren (Stockholm/SE), J. Lahmann (Lübeck/DE), M. Difilippantonio, T. Ried (Bethesda/US), H. Jörnvall, G. Auer (Stockholm/SE), J. K. Habermann (Lübeck/DE) P140 MALDI-imaging reveals TYB4 to classify diploid from aneuploid colon cancer T. Gemoll, S. Strohkamp, K. Schillo, C. Thorns, J. K. Habermann (Lübeck/DE) P141 A data independent strategy for a multi-omic approach to investigate obesity treatment within a mouse model G. Kramer, N. Dekker (Amsterdam/NL), L. A. Gethings (Wilmslow/UK), V. Lee, R. Beynon (Liverpool/UK), J. I. Langridge, J. P. Vissers (Wilmslow/UK), J. M. Aerts (Amsterdam/NL) P142 New proteomic biomarkers for psoriasis and psoriasis arthritis R. Büchler, J. Reindl, P. Muckova, S. Wendler, S. Opitz, T. Krüger, N. Krieg , J. Pesek, S. Nemitz, J. Norgauer, H. Rhode (Jena/DE) P143 Acute respiratory infection – biomarker search in an animal model disease P. Muckova, R. Buechler, S. Wendler, P. Reinhold, H. Rhode (Jena/DE) P144 Characterisation of regenerative phenotype of unrestricted somatic stem cells from human umbilical cord blood by functional secretome analysis K. Stühler, J. Schira, H. Falkenberg, M. Hendricks, G. Kögler, D. M. Waldera-Lupa (Düsseldorf/DE), H. E. Meyer (Dortmund/DE), H.-W. Müller (Düsseldorf/DE) P145 Identification of low molecular weight biomarkers of prostate cancer by 2-D gel electrophoresis J. Alruwaili (Arar/SA) Proteomics in Biotechnology P192 Evaluating sample preparation for mesenchymal stem cell secretome analysis – a 2D gel electrophoretic approach A.-K. Koch, V. Charwart, C. Kasper, M. Marchetti-Deschmann (Vienna/AT)

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P193 Comparative proteomic analysis of human osteosarcoma and pulmonal metastasis T. Gemoll, F. Epping, L. Heinrich, B. Fritzsche, U. J. Roblick, S. Szymczak, S. Hartwig, R. Depping, H.-P. Bruch, C. Thorns, S. Lehr, A. Paech, J. K. Habermann (Lübeck/DE) P194 A comparative study on the quantification of non-biological protein modifications using mass spectrometry E. Soher, C. Stephan, M. Marchetti-Deschmann (Vienna/AT) P195 Cattle subfertility and bacterial infection: uterine epithelial cells response after LPS exposure C. Piras, A. Soggiu (Milan/IT), V. Greco (Rome/IT), Y. Guo (Uppsala/SE), L. Bonizzi (Milan/IT), A. Urbani (Rome/IT), P. Humblot (Uppsala/SE), P. Roncada (Milan/IT) P196 The separation and identification of glycans using an ultra high resolution column and an ultra high resolution mass spectrometer J. Saba, K. Hempel, M. Oppermann (Dreiech/DE) P197 Understanding organic matrix proteins of oyster shell in context with climate change and biomineralization A. Upadhyay, D. Ramadoss, V. Thiyagarajan (Hong Kong/CN) Quantitative Proteomics I P199 Impact of conventional cigarette smoking and cessation on the lung proteome of ApoE-/- mice quantified by Tandem Mass Tag (TMT) LC MS/MS approach C. Nury, T. Schneider, S. Dijon, B. Titz, A. Elamin, F. Martin, M. Cabanski (Neuchatel/CH), B. Phillips (Singapore/SG), P. Vanscheeuwijck, N. V. Ivano, J. Hoeng, M. C. Peitsch (Neuchatel/CH) P200 Proteasome isoforms exhibit only quantitative differences in cleavage and epitope generation M. Mishto (Berlin/DE) P201 Integrated metabolome and proteome profiling of ρ0 Cells I. Gielisch, D. Meierhofer (Berlin/DE), I. Wittig (Frankfurt/DE) P202 Label free MS1-based quantitative analysis of mouse skeletal muscle after 30 days in zero-gravity on the bion-M1 biosatellite G. Tascher (Strasbourg, Paris/FR), S. Blanc, A. Van Dorsselaer, G. Gauquelin-Koch (Paris/FR), F. Bertile (Strasbourg/FR) P203 Absolute protein quantification of clinically relevant metabolizing enzymes by mass spectrometry-based targeted proteomics D. Busch, C. Gröer, S. Oswald, W. Siegmund (Greifswald/DE)

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P204 Verification of a parkinson’s disease protein signature by multiple reaction monitoring LC-MS T. Alberio (Insubria/IT), K. McMahon, M. Cuccurullo, L. A. Gethings, G. Weibchen, C. Lawless (Manchester/UK), M. Zibetti, L. Lopiano (Torino/IT), J. P. Vissers (Wilmslow/UK), M. Fasano (Insubria/IT) P205 High quantification efficiency for discovery and validation approaches on a Q-TOF platform S. Kaspar, U. Schweiger-Hufnagel (Bremen/DE), P.-O. Schmit (Wissembourg/FR) P206 Performance investigation of a novel integrated microfluidics platform in high-throughput LC-MS MRM disease protein marker verification C. Hughes, D. Turkovic, J. P. Vissers, L. A. Gethings, J. I. Langridge (Wilmslow/UK) P207 Method development for the quantification of cardiac biomarkers by targeted mass spectrometry F. Polten, K. Wollert, A. Pich (Hannover/DE) P208 A new TMT-SRM 16plex neurodegeneration assay to measure biomarkers in cerebrospinal fluid C. Lößner (Frankfurt a. M./DE), M. Ward (London/UK), H. Zetterberg, J. Gobom, K. Blennow (Gothenburg/SE), I. Pike (London/UK) P209 Extracellular matrix and intracellular proteome of primary skin fibroblasts in 2D compared to 3D tissue culture R. Tölle (Freiburg i. Br./DE) P210 Secretomics of the endometrium during diapause and reactivation in the tammar wallaby (Macropus eugenii) F. C. w, C.-S. Ang, N. Williamson, M. Renfree, D. Gardner, G. Shaw (Melbourne/AU) P211 Poly(ADP-ribose) glycohydrolase silencing down-regulates TCTP and Cofilin-1 associated with metastasis in benzo(a)pyrene carcinogenesis H. Huang, X. Li, Z. Zhuang, J. Liu (Shenzhen/CN), X. Li (West (Lafayette/US)), W. Gao, Y. Liu (Shenzhen/CN) P212 Extending the limits of quantitative proteome profiling with data-independent acquisition and application to acetaminophen treated 3D liver microtissues R. Bruderer, O. Bernhardt, T. Gandhi, S. M. Miladinovic (Schlieren/CH), L.-Y. Chen (West Lafayette/US), S. Messner, T. Ehrenberger, V. Zanotelli, Y. Butscheid, C. Escher (Schlieren/CH), O. Vitek (West Lafayette/US), O. Rinner, L. Reiter (Schlieren/CH)

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P213 Clinical proteomic assays based on high-resolution mass spectrometry B. Domon, S. Gallien, Y. J. Kim, G. Berchem (Strassen/LU) P214 DIA and Spectronaut: comprehensive and precise proteome profiling L. Reiter, O. Bernhardt, T. Gandhi, R. Bruderer (Schlieren/CH)

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P215 Quantitative strategies for multiplexed high-throughput protein analysis A. Bhangu-Uhlmann, W. Deininger (Bad Abbach/DE)

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Poster Sessions

Poster Session B • Monday, 23 March 2015, 15:00–16:30 Affinity Proteomics Imaging Mass Spectrometry Technologies Plant and Microbial Proteomics I Post-Translational Modifcations I Proteomics for Human Health II

P001–P013 (see p. 38 ff.) P050–P055 (see p. 39 ff.) P056–P065 (see p. 40 ff.) P066–P079 (see p. 41 ff.) P092–P107 (see p. 42 ff.) P146–P169 (see p. 44 ff.)

Foyer ground floor Foyer ground floor Foyer first floor Foyer first floor Foyer first floor Atrium

Affinity Proteomics P001 DNA-Affinity purification followed by mass spectrometry (AP-MS): application to the analysis of transcriptional regulators interacting with a defined DNA sequence A. Tacheny, M. Dieu, P. Renard (Namur/BE) P002 Unbiased allele-specific quantitative proteomics unravels molecular mechanisms influenced by cis-regulatory genomic variations S. Hauck (Neuherberg/DE), H. Lee (Freising/DE), C. von Törne (Neuherberg/DE), M. Claussnitzer (Freising/DE), H. Grallert (Neuherberg/DE), I. Dahlman, P. Arner (Stockholm/SE), H. Hauner, H. Laumen (Freising/DE) P003 Label-free quantitative proteomic analysis of LRRK2-associated protein interaction networks in neuronal cell model systems A. Meixner, D. C. Schöndorf (Tübingen/DE), M. Deleidi, T. Gasser (Tübingen/DE), M. Ueffing (Tübingen/DE), C. J. Gloeckner (Munich/DE) P004 Stoichiometry determination of the intraflagellar transport complex A by absolute auantification Y. Wissinger (Tübingen/DE), J. Fuchs, N. Mischerikow (Vienna/AT), A. Meixner (Tübingen/DE), K. Mechtler (Vienna/AT), M. Ueffing, K. Boldt (Tübingen/DE) P005 Analysis of virus attachment complexes by combining native gel electrophoresis and nLC-MS/MS with cluster analysis of the protein co-migration behaviour J. Doellinger, A. Nitsche (Berlin/DE) P006 Chemical proteomic analysis of cytokinin molecular partners in Arabidopsis R. Simerský, V. Skalický, R. Lenobel, V. Mik, I. Chamrád, M. Srnad (Olomouc/CZ) P007 Comparison of the compounds VI16832 and KiNet-1 for small molecule affinity chromatography of protein kinases K. Morgenroth, E. Richter, M. Harms, J. Mostertz, F. Hochgräfe (Greifswald/DE)

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Poster Sessions


P008 On terminal alkynes that can react with active-site cysteine nucleophiles in proteases title of the contribution S. Goerdayal (Utrecht/NL), R. Ekkebus, S. van Kasteren (Amsterdam/NL), A. Scholten, A. J. R. Heck (Utrecht/NL), H. Ovaa (Amsterdam/NL) P009 Chemoproteomics: does kinase abundance after enrichment reflect kinase activity? B. Ruprecht (Freising, Munich/DE), J. Zecha, S. Heinzlmeir (Freising, Heidelberg/DE), G. Medard (Freising/DE), S. Lemeer (Freising, Utrecht/DE,NL), B. Kuster (Freising, Heidelberg, Munich/DE) P010 Chemically cleavable linkers for target identification S. Verhelst (Dortmund/DE) P011 Presenting AFFIRM – a novel immunoaffinity platform that combines recombinant antibody fragments and LC-SRM analysis for sensitive multiplexed protein analysis S. Waldemarson, A. Säll, H. Persson (Lund/SE) P012 Abduction of protein complexes from mammalian cells by Virotrap S. Eyckerman, K. Titeca, E. Van Quickelberghe, E. Cloots, A. Verhee, N. Samyn, L. De Ceuninck, E. Timmerman, D. De Sutter, S. Lievens, S. Van Calenbergh, J. Tavernier, K. Gevaert (Gent/BE) P013 In vivo interaction mapping in C. elegans embryos J.-X. Chen (Berlin/DE), P. G. Ciprian, D. Mecenas, K. C. Gunsalus, F. Piano (New York/US), M. Selbach (Berlin/DE) Imaging P050 3D molecular histology – correlation of 3D histology and 3D molecular imaging H. Thiele, J. Lotz (Lübeck/DE), F. Hoffmann (Lübeck, Jena/DE), S. Heldmann (Lübeck/DE), D. Trede (Bremen/DE), G. Ernst, O. Guntinas-Lichius, F. von Eggeling (Jena/DE) P051 MALDI-Imaging mass spectrometry: discrimination of pathophysiological processes in traumatized skeletal muscle H. Thiele (Lübeck/DE), O. Klein, G. Nebrich, K. Strohschein, G. N. Duda, T. Winkler (Berlin/DE), D. Trede (Bremen/DE) P052 Lipid visualization and identification through collision cross section aided correlation of MALDI imaging and MS/MS fragmentation data sets M. Towers, E. Claude, I. Edwards, J. P. Vissers (Wilmslow/UK)

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Poster Sessions

P053 Classification of prostate cancer tissue samples into prognostic subgroups – Testing the potential of MALDI MSI as a clinical tool B. Beine (Dortmund/DE), D. Dietrich, G. Kristiansen (Bonn/DE), H. E. Meyer (Dortmund/DE), B. Sitek (Bochum/DE), C. Henkel (Dortmund/DE) P054 xiNET – a visualization tool for exploring cross-linking/mass spectrometry results C. Combe (Edinburgh/UK), C. Forbrig (Berlin/DE), J. Rappsilber (Berlin, Edinburgh/DE, UK) P055 Multiple mass spectrometric imaging analyses from single tissue section with Desorption Electrospray Ionization (DESI) on a oa-TOF mass spectrometer I. Edwards, E. Claude, E. Jones (Wilmslow/UK) Mass Spectrometry Technologies P056 Increasing depth of coverage in data independent acquisition D. Waidelich, J. Dojahn, M. Macht (Darmstadt/DE), B. Collins, R. Aebersold (Zurich/CH), C. Hunter (Redwood City/US) P057 Exploring impact of dynamic accumulation for improving MS/MS quality of QqTOF data C. Baumann, D. Merkel, J. Dojahn, M. Macht (Darmstadt/DE), N. Bloomfield (Redwood City/ US), S. Seymour (Redwood City, San Francisco/US), C. Hunter (Redwood City/US) P058 Characterization of an improved ultra-high resolution quadrupole time of flight (UHR-TOF) instrument for proteomics applications S. Kaspar, M. Lubeck, A. Michalski, O. Raether, C. Gebhardt (Bremen/DE) P059 Identification of complex I assembly intermediates in TMEM126A and TMEM126B deficient cells V. Strecker, L. Bleier, M. Steger, H. Heide, B. Schwamb, M. Zörnig, S. Dröse (Frankfurt a. M./DE), U. Brandt (Nijmegen/NL), I. Wittig (Frankfurt a. M./DE) P060 A complete workflow for achieving high accuracy multiplexed N-terminomics data using TMT10plex P. Saliba (Dreieich/DE) P061 MALDI in-source decay for characterization of engineered proteins M. Michalak, S. Fiedler (Heidelberg/DE), H.-J. Gabius (Munich/DE), J. Kopitz, M. Schnölzer (Heidelberg/DE) P062 Comparison of human whole saliva samples of patients suffering from chronic periodontitis using different mass spectrometric approaches A. Murr (Greifswald/DE), M. Kipping (Eschborn/DE), M. Gesell Salazar, E. Hammer, V. M. Dhople, T. Kocher, U. Völker (Greifswald/DE)

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Poster Sessions


P063 A quality control tool for LCMS based Proteomics: MQQC H. Zauber, M. Selbach (Berlin/DE) P064 Comparison of data-dependent and data-independent acquisition techniques for relative protein quantification A. Dörrbaum, G. Dürnberger S. Opravil, M. Schutzbier, Z. Demianova, K. Mechtler (Vienna/AT) P065 Detection of stress responses in lactic acid bacteria Lactobacillus paracasei ssp. paracasei and Lactobacillus brevis by MALDI-TOF mass spectrometry A.-S. Schott, B. C. Schurr, J. Behr, R. F. Vogel (Freising/DE) Plant and Microbial Proteomics I P066 Mechanism of drug resistance in Candida albicans C. YingYing (Shanghai/CN) P067 Comparative proteome analysis of differentially expressed proteins in rubber latex of Hevea brasiliensis from Phytophthora resistant (BPM24) and susceptible (RRIM600) clones P.-O. Havanapan (Nakhonpathom/TH), N. Pongthanom (Nakhonpathom/TH), A. Bourchookarn (Pattani/TH), C. Kriitanai (Nakhonpathom/TH) P068 Comparative proteomic analysis reveals involvement of the Tor pathway in iron regulation of the human-pathogenic fungus Aspergillus fumigatus T. Krüger, C. Baldin, V. Valiante (Jena/DE), H. Haas (Innsbruck/AT), O. Kniemeyer, A. A. Brakhage (Jena/DE) P069 Metaproteomics: analysing the active fraction of the gut and rumen associated microbiota J. Seifert, B. Tilocca, S. Deusch (Stuttgart/DE) P070 Mining cross-reactive allergens from coconut and date palm pollen through homology driven proteomics B. Saha, S. Gupta Bhattacharya (Kolkata/IN) P071 Physiological role of ETHE1 for A. thaliana seed metabolism C. Lorenz, T. Busch (Hannover/DE), D. Gerhard (Christchurch/NZ), H.-P. Braun, T. Hildebrandt (Hannover/DE) P072 Reduction of the signal suppression effects in MALDI O. Sedo (Brno/CZ), A. Nemec, L. Krizova (Prague/CZ), Z. Zdráhal (Brno/CZ)

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Poster Sessions

P073 Nitrogen deficiency responses of Solanum tuberosum genotypes revealed by proteomic and phosphoproteomic approaches A. Jozefowicz (Gatersleben/DE ), S. Kaspar (Bremen/DE), A. Matros, H.-P. Mock (Gatersleben/DE) P074 Host targets of luteoviral protein in plants using BioID M. Kraner, U. Sonnewald (Erlangen/DE) P075– withdrawn P076– withdrawn P077 – withdrawn P078 Semi-in vivo tobacco pollen tube secretome D. Potesil (Brno/CZ), S. Hafidh, J. Fila, V. Capkova (Prague/CZ), E. Srbova, K. Stejskal (Brno/CZ), D. Honys (Prague/CZ), Z. Zdráhal (Brno/CZ) P079 Food safety: improve knowledge of secretome during bacterial competition P. Roncada, I. Alloggio, C. Piras (Milan/IT), V. Greco (Rome/IT), A. Soggiu (Milan/IT), N. Losio (Brescia/IT), A. Urbani (Rome/IT), L. Bonizzi (Milan/IT) Post-Translational Modifcations I P092 Analysis of posttranslational cysteine modifications in a neural differentiation model by redox proteomics K. Nau, K. Stühler, G. Poschmann (Düsseldorf/DE) P093 Biosensing of extracellular complexes of glycosylphosphatidylinositol-anchored proteins and phospholipids as biomarkers for stress-induced diseases G. Müller, M. Tschöp (Garching-Hochbrück/DE) P094 Using SWATH™ acquisition for characterization and quantification of the epigenetic histone modifications J. Dojahn (Darmstadt/DE), S. Mollah, C. Hunter, E. Johansen, L. Xiong (Redwood City/US), B. A. Garcia (Philadelphia/US) P095 PFP-based separation improves characterization of histone modification status by mass spectrometry G. Lochmanová (Brno/CZ), T. Pani-Jankovi, A. Fichtenbaum (Vienna/AT), Z. Zdráhal (Brno/CZ), G. Mitulović (Vienna/AT) P096 Comparison of ERLIC with a new mixed-mode strategy using WCX-RPC for phosphopeptide separation prior to mass spectrometry C. Regl, L. Weilnböck, C. Huber (Salzburg/AT)

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Poster Sessions


P097 Regulatory signaling pathways underlying the dynamic translocation of mucins upon carbachol stimulation E. Berger, H. Schneider, T. Pelaseyed, G. C. Hansson (Gothenborg/SE) P098 Using multiple collision energies to improve N- and O-glycopeptide identification by LC-MS/MS U. Schweiger-Hufnagel, S. Pengelley (Bremen/DE), H. Hinneburg, D. Kolarich (Potsdam/DE) P099 Phosphorylation-mediated host signalling during interaction with staphylococcal alpha-toxin or Staphylococcus aureus E. Richter, M. Harms, R. Nölker, K. Ventz, J. Mostertz, F. Hochgräfe (Greifswald/DE) P100 Quantitative separation of protein isoforms by free flow electrophoresis G. Weber, R. Wildgruber, U. Sukop-Köppel, L. Eichacker (Feldkirchen/DE) P101 Multiplex quantitation of reversible thiol modifications in proteins with iodoTMT reagents S. Blankenburg, R. Nölker, D. Ulbrich, J. Mostertz, F. Hochgräfe (Greifswald/DE) P102 Mass spectrometry-based analysis of thiol-redox and phosphorylation cross talk in human bronchial epithelial cells J. Mostertz, R. Nölker, A. M. Klingebeil, D. Ulbrich, S. Blankenburg, E. Richter, F. Hochgräfe (Greifswald/DE) P103 MALDI and ESI identify orthogonal parts of the phosphoproteome B. Ruprecht (Freising/DE), C. Roesli (Heidelberg/DE), S. Lemeer (Freising, Utrecht/DE, NL), B. Kuster (Freising/DE) P104 Generation of biomarkers for the detection of ADP-ribosylated proteins during cellular stress V. Bilan, M. O. Hottiger (Zurich/CH), G. Hopfgartner (Geneva/CH) P105 Development of a phosphoproteomic workflow for the analysis of crosstalk between insulin signaling and selected metabolic pathways M. Rahm, A. Schaefer, M. Ueffing (Tübingen/DE), S. M. Hauck (Oberschleißheim/DE) P106 Analysis of post translational modifications using DIA with high resolution MS1 and retention time prediction R. Bruderer, T. Gandhi, O. Bernhardt, L. Reiter (Schlieren/CH) P107 Proteomic analysis of cysteine persulfides S. Longen, F. Richter, K.-F. Beck, I. Wittig, J. Pfeilschifter (Frankfurt a. M./DE)

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Poster Sessions

Proteomics for Human Health II P146 Coupling SPRi and MALDI MS for interaction analysis U. Anders, J. Schaefer (Zurich/CH), C. Frydman, F. Hibti (Palaiseau/FR), C. Schuhmacher (Unterhaching/DE), D. Suckau (Bremen/DE), A. Plückthun, R. Zenobi (Zurich/CH) P147 Proteomic profiling of patient-derived CD4+ T-cells for biomarker discovery in type 1 diabetes M. Lepper, C. von Törne, E.-M. Sedlmeier, A.-G. Ziegler, S. Hauck (Neuherberg/DE) P148 Platelet proteome changes in stable angina pectoris patients induced by aspirin treatment P. Majek, K. Pecankova, Z. Riedelova-Reicheltova, M. Maly, M. Oravec, J. Suttnar, J. Dyr (Prague/CZ) P149 Platelet-protein-profiling as a screening method for colorectal cancer S. Strohkamp, T. Gemoll, S. Johanning (Lübeck/DE), S. Hartwig, S. Lehr, S. Becker, B. Franzén (Stockholm/SE), U. J. Roblick, T. Keck (Lübeck/DE), G. Auer (Stockholm/SE), J. K. Habermann (Lübeck/DE) P150 Peripheral blood mononuclear cell proteome changes in patients with myelodysplastic syndrome K. Pecankova, P. Majek, J. Cermak, J. Dyr (Prague/CZ) P151 Drug-dependent regulation of B-Raf containing macromolecular protein complexes B. Diedrich, K. Rigbolt, M. Röring, T. Brummer, J. Dengjel (Freiburg/DE) P152 A kit for mass-spectrometry based absolute quantification of metabolic enzymes K. Schnatbaum, S. Kempa, G. Mastrobuoni, F. Bindel, P. Ensle, J. Avramova-Nehmer, H. Wenschuh, U. Reimer (Berlin/DE) P153 Analysis of cerebrospinal fluid using high resolution mass spectrometry S. Galozzi, K. Barkovits, K. Marcus (Bochum/DE) P154 Molecular and proteomic characterization of two primary cultures recovered from the ascites of mexican ovarian adenocarcinoma patients O. L. Garibay-Cerdenares, V. Hernández-Ramírez, J. C. Osorio-Trujillo, B. Chávez Munguía, A. González-Robles (Mexico/MX), M. Hernández Ortíz, S. M. Encarnación Guevara (Cuernavaca/MX), D. Gallardo-Rincón, P. Talamás-Rohana (Mexico DF/MX) P155 Proteomic changes of Caco-2 cells after silver nanoparticle treatment S. Juling, D. Lichtenstein, L. Böhmert, S. Selve, A. Niedzwiecka, M. Schümann, H. Sieg, A. Thünemann, E. Krause, A. Braeuning, A. Lampen (Berlin/DE)

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Poster Sessions


P156 Comparison of two different iron oxide nanoparticle protein corona after isolation from complex HepG2 cell lysate S. Juling, D. Lichtenstein, J. David, H. Sieg, C. Rozycki, B. Niemann, A. Braeuning, A. Lampen (Berlin/DE) P157 A first proteome wide prediction of the dynamics of insulin resistance unveils systems wide mechanisms behind type II diabetes R. Magnusson, E. Nyman, M. Gustavsson, P. Strålfors, G. Cedersund (Linköping/SE) P158 Proteomic approach to study a couple of monozygotic twins discordant for the chronic fatigue syndrome: focus on mitochondria involvement F. Ciregia (Pisa, Rome/IT), L. Giusti (Pisa/IT), L. Kollipara, R. P. Zahedi (Dortmund/DE), M. Ronci (Rome/IT), L. Bazzichi, C. Giacomelli (Pisa/IT), A. Urbani (Rome/IT), A. Sickmann (Dortmund/DE), A. Lucacchini (Pisa/IT) P159 A refined mass spectrometry based draft of the human proteome H. Hahne (Freising/DE), M. Wilhelm (Freising, Walldorf/DE), J. Schlegl (Walldorf/DE), A. Moghaddas Gholami (Freising/DE), M. Lieberenz (Walldorf/DE), M. M. Savitski (Heidelberg/DE), B. Hallström (Stockholm/SE), E. Ziegler (Walldorf/DE), T. Mathieson (Heidelberg/DE), L.-H. Li, D. Wang (Freising/DE), L. Butzmann, S. Gessulat (Walldorf/DE), H. Marx, S. Lemeer (Freising/DE), K. Schnatbaum, U. Reimer, H. Wenschuh (Berlin/DE), A. Asplund (Uppsala/SE), M. Mollenhauer, J. Slotta-Huspenina (Freising/DE), J.-H. Boese, A. Gerstmair (Walldorf/DE), M. Bantscheff (Heidelberg/DE), F. Pontén (Uppsala/SE), M. Uhlen (Stockholm/SE), F. Faerber (Walldorf/DE), B. Kuster (Freising/DE) P160 Systematic exploration of the ciliary protein landscape by large-scale affinity proteomics K. Boldt (Tübingen/DE), J. van Reeuwijk (Nijmegen/NL), Q. Lu (Heidelberg/DE), K. Koutroumpas (Evry/FR), N. Horn (Tübingen/DE), S. van Beersum (Nijmegen/NL), Y. Texier (Munich/DE), M. T. Nguyen (Nijmegen/NL), The Syscilia Consortium EU (Brussels/BE), F. Kepes (Evry/FR), R. B. Russell (Heidelberg/DE), M. Ueffing (Tübingen/DE), R. Roepman (Nijmegen/NL) P161 Clostridium difficile toxins A and B glucosylate small GTPases and alter the proteome of human cells J. Junemann, J. Erdmann, J. Zeiser, N. Jochim, I. Just, R. Gerhard, A. Pich (Hannover/DE) P162 Time-resolved quantitative phosphoproteomics to study platelet signaling in health and disease F. Beck (Dortmund/DE), J. Geiger (Würzburg/DE), S. Gambaryan (S. Petersburg/RU), S. Loroch (Dortmund/DE), O. Pötz (Reutlingen/DE), C. Fufezan (Müsnter/DE), J. Heemskerk (Maastricht/NL), U. Walter, A. Sickmann (Dortmund, Mainz/DE), R. P. Zahedi (Dortmund/DE)

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Poster Sessions

P163 Proteomic study of SET-mediated phosphorylation and regulatory analysis of nucleolin in trichloroethylene-induced hepatic cytotoxicity J. Liu, X. Ren, B. Xia, W. Liu, X. Yang (Shenzhen/CN) P164 A plasma proteomics study of experimental thyrotoxicosis in humans B. Engelmann (Greifswald/DE), A.-L. Dirk (Lübeck/DE), E. Hammer, N. Friedrich (Greifswald/DE), D. Führer (Essen/DE), G. Homuth (Greifswald/DE), G. Brabant (Lübeck/DE), U. Völker (Greifswald/DE) P165 Disease proteomics to study the deregulation of molecular mechanisms in inherited skin fragility K. Thriene (Freiburg i. Br./DE) P166 Investigation of platelet proteome changes in human endotoxemia model G. Schmidt (Vienna/AT) P167 Investigation of the platelet proteome in a human endotoxemia model G. Schmidt, A. Assinger, W. Schrottmaier, B. Jilma, M. Zellner (Vienna/AT) P168 Identification of breast cancer biomarker signature for risk classification using a targeted proteomics approach U. Korf, J. Sonntag (Heidelberg/DE), A. Wachter, T. Beissbarth (Göttingen/DE) P169 Longitudinal study of urinary proteome using targeted LC-MS approach N. Khristenko (Strassen/LU), I. Larina (Moscow/RU), B. Domon (Strassen/LU)

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Poster Sessions


Poster Session C • Tuesday, 24 March 2015, 15:00–16:30 Plant and Microbial Proteomics II P080–P091 (see p. 47 ff.) Post-Translational Modifcations II P108–P122 (see p. 48 ff.) Proteomics for Human Health III P170–P190 (see p. 49 ff.) Quantitative Proteomics II P216–P232 (see p. 52 ff.) Structural Proteomics P233–P238 (see p. 53 ff.) Subcellular Proteomics P239–P251 (see p. 54 ff.)

Foyer first floor Foyer first floor Atrium Atrium Atrium Atrium

Plant and Microbial Proteomics II P080 Quantitative ChaFRADIC reveals that mitochondrial Icp55 cleavage specificity is conserved between yeast and Arabidopsis S. Venne (Dortmund/DE), C. Carrie, J. Soll (Martinsried/DE), R. P. Zahedi (Dortmund/DE) P081 Efficient purification of dilute samples for quantitative proteomics studies F. Bonn, J. Bartel, K. Büttner, M. Hecker, A. Otto, D. Becher (Greifswald/DE) P082 Characterization of the protein complexes associated to the phosphatidylinositol 3-kinase during Phaseolus vulgaris-rhizobia interaction C. V. Dorantes Torres, G. Estrada Navarrete, F. E. Sanchez Rodriguez, N. D. J. Cruz Mireles (Cuernavaca/MX) P083 Impact of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) in the routine diagnostic microbiology laboratory in Alexandria University, Egypt N. Abouseada, M. Moheb, P. Moez (Alexandria/EG) P084 Why database matters: impact of sequence databases on metaproteome analysis of a mock microbial mixture A. Tanca, A. Palomba (Alghero/IT), M. Deligios (Sassari/IT), T. Cubeddu, C. Fraumene, G. Biosa, D. Pagnozzi, M. F. Addis (Alghero/IT), S. Uzzau (Alghero, Sassari/IT) P085 A straightforward and efficient analytical pipeline for metaproteome characterization A. Tanca, A. Palomba, S. Pisanu (Alghero/IT), M. Deligios (Sassari/IT), C. Fraumene (Alghero/IT), V. Manghina (Sassari/IT), D. Pagnozzi, M. F. Addis (Alghero/IT), S. Uzzau (Alghero, Sassari/IT) P086 Enrichment or depletion? The impact of stool pretreatment on metaproteomic characterization of the human gut microbiota A. Tanca, A. Palomba, S. Pisanu (Alghero/IT), M. F. Addis (Alghero/IT), S. Uzzau (Alghero, Sassari/IT)

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Poster Sessions

P087 Functional proteomics on Arabidopsis MAP3K mutants and MAP2K overexpressor line T. Takac, P. Vadovic, S. Bekesova (Olomouc/CZ), T. Pechan (Mississippi/US), I. Luptovciak, V. Smekalova, M. Ovecka, J. Samaj (Olomouc/CZ) P088 Quantifying protein dynthesis and degradation in Arabidopsis P. Ihmor, W. Gruissem, M. Robinson, K. Baerenfaller (Zurich/CH) P089 Global and quantitative study of protein complexes in Arabidopsis thaliana M. Górka, A. Graf, D. Walther (Potsdam/DE) P090 A redox dependent interaction between ROC4 and GWD might play a role in regulating starch degradation in plants C. Swart, A. Skeffington (Potsdam/DE) P091 Investigation of Hülle cells from the filamentous fungus Aspergillus nidulans B. Dirnberger (Göttingen/DE) Post-Translational Modifcations II P108 A proteomic investigation of proteasome malfunctioning J. Demmers (Rotterdam/NL) P109 A Strategy for high throughput lysine methylation screening F. Richter (Frankfurt a. M., Freiburg i. Br./DE), G. Mittler (Freiburg i. Br./DE), I. Wittig (Frankfurt a. M./DE) P110 Identification of targets of the Ser/Thr kinase PrkC from Staphylococcus aureus by quantitative phosphoproteomics M. Steger, A. Schlosser (Würzburg/DE) P111 Subtilisin – an alternative enzyme for PTM research H. Gonczarowska-Jorge, M. DellÁica, C. Dickhut, R. P. Zahedi (Dortmund/DE) P112 A MS/MS based workflow for the detection of unknown stable protein adducts M. Mueller, A. Acosta-Martin, P. Antinori Malaspina, O. Horlacher, A. Scherl, F. Lisacek, P. Lescuyer (Geneva/CH) P113 Identification of interaction networks and posttranslational modifications of mutant and wild type IDH1 in glioma cell lines N. Overbeck, A. Stefanski, V. Scherbaum, C. B. Knobbe-Thomsen, K. Stühler (Düsseldorf/DE)

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Poster Sessions


P114 Global analysis of the impact of protein phosphorylation on mRNA-binding ability K. Imami, M. Milek, M. Landthaler, M. Selbach (Berlin/DE) P115 Post-translational modification analysis of A. nidulans histone 3 C. Gruber (Vienna/AT), A. Gacek-Matthews, J. Strauss (Tulln/AT), F. Altmann (Vienna/AT) P116 Identification of the myofibrillar Z-disc as a nodal point in skeletal myocyte signaling by large-scale phosphoproteomics L. Reimann, H. Wiese, A. L. Fricke (Freiburg/DE), Y. Leber, Z. Orfanos (Bonn/DE), G. Radiziwill (Freiburg/DE), P. van der Ven, D. O. Fürst (Bonn/DE), B. Warscheid (Freiburg/DE) P117 Advances in antibody-based proteomic analysis M. P. Stokes, B. Textor, H. Gu, C. L. Farnsworth, J. M. Ren, K. A. Lee, J. C. Silva (Danvers/US) P118 One for all? TiO2 and IMAC enrichment of phospho- and glycopeptides from serum E. Klement, T. Raffai, Z. Darula, E. Hunyadi-Gulyas, K. F. Medzihradszky (Szeged/HU) P119 N- and O-glycopetide analysis from HCD data Z. Darula, E. Hunyadi-Gulyas (Szeged/HU), M. Bern (San Carlos, Palo Alto/US), K. F. Medzihradszky (Szeged/HU) P120 Wound fluid degradomics: system-level insight into protease dynamics in cutaneous wound healing U. auf dem Keller, F. Sabino, O. Hermes, F. Egli, T. Kockmann, P. Schlage (Zurich/CH), P. Croizat (Heidenheim/DE), J. Kizhakkedathu (Vancouver/CA), H. Smola (Heidenheim/DE) P121 Quantitative MS-based analysis of histone modification patterns as markers for response to HDAC inhibitors in breast cancer R. Noberini, C. Miccolo, G. Pruneri, S. Chiocca, S. Minucci, T. Bonaldi (Milan/IT) Proteomics for Human Health III P170 Proteome analysis and comparative study of normal endometrial stem cells, endometriosis stem cells S. Heidari-Keshel, M. Rezaei-Tavirani, M. Ebrahimi (Tehran/IR)

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Poster Sessions

P171 Moroccan cases with Alzheimer disease: genetic and proteomic aspects N. El Kadmiri (Casablanca/MA) P172 Analysis of protein-protein interactions in living cell using NanoBRET™ technology T. Machleidt (Madison/US), C. Woodroofe (San Luis Obispo/US), J. Mendez, N. Murphy (Madison/US), C. End (Mannheim/DE), K. Zimmermann, P. Otto, M. Schwinn, M. Robers, D. Daniels (Madison/US), T. Kirkland (San Luis Obispo/US), K. Wood (Madison/US) P173 Cigarette smoke treatment induces alterations in protein networks of AMD risk genes J. Merl-Pham, F. Gruhn, N. Senninger, G. John-Schuster, A. Yildirim, S. Hauck (Neuherberg/DE) P174 Key event in human allergic contact dermatitis – adduct formation of the common xenobiotic human contact sensitizer methylisothiazolinone H. Regine, S. Nicolai, A. Luch, H.-J. Thierse (Berlin, Heidelberg/DE) P175 Mass spectrometry assays of plasma biomarkers to predict radiographic progression of knee osteoarthritis M. Oppermann, M. Lopez (Boston/US) P176 Host cell protein synthesis response to infection with different influenza A virus strains K. Eichelbaum, B. Bogdanow, A. Sadewasser, K. Paki, T. Wolff, M. Selbach (Berlin/DE) P177 Proteomic alterations in human renal epithelial cells exposed to nephrotoxins L. Kollipara (Dortmund/DE), A. Limonciel (Innsbruck/AT), A. Sickmann (Dortmund/DE), P. Jennings (Innsbruck/AT) P178 Identification of widespread alterations in RNA metabolism in sporadic inclusion-body myositis using SILAC-based quantitative proteomics T. Opialla, M. Berger, A. Marg, S. Kempa, S. Spuler (Berlin/DE) P179 Measuring Wnt-pathway activity in cancer models O. Pötz (Reutlingen/DE), C. Esdar (Darmstadt/DE), C. Sommersdorf (Reutlingen/DE), R. Schneider (Darmstadt/DE), T. O. Joos (Reutlingen/DE) P180 Comprehensive analysis of acute- and late-cellular response to photon and carbon irradiation using quantitative proteomics M. Winter, T. Rackwitz, P. Seidel, C. Zhou, R. Mayer, U. Warnken, A. Abdollahi, M. Schnölzer (Heidelberg/DE)

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Poster Sessions


P181 Critical comparison of sample preparation strategies for shotgun proteomic analysis of formalin-fixed, paraffin-embedded samples A. Tanca, M. Abbondio (Alghero, Sassari/IT), S. Pisanu, D. Pagnozzi (Alghero/IT), S. Uzzau (Alghero, Sassari/IT), M. F. Addis (Alghero/IT) P182 Host-adaptation of Burkholderia pseudomallei alters metabolism and virulence factors: a global comparative proteomic analysis V. Mariappan, K. M. Vellasamy, J. Vadivelu (Kuala Lumpur/MY) P183 Investigation of the effect of cigarette smoke exposure on changes in the mitochondrial proteome of cultured human retinal pigment epithelium cells U. Ohmayer, J. Merl-Pham (Neuherberg/DE), M. Gorza, F. Perocchi (Munich/DE), S. Hauck (Neuherberg/DE) P184 Identification of galectin-specific glycoprotein ligands on RPE and RMG cells J. Obermann, S. Hauck (Neuherberg/DE), C. Alge-Priglinger (Munich/DE) P185 Proteome analysis and comparative study of normal endometrial stem cells, endometriosis stem cells and bone marrow mesenchymal stem cells S. Heidari-Keshel (Tehran/IR) P186 Proteomic profiling of Moroccan cobra Naja haje legionis venom and screening of its pharmacological activities I. Malih (Casablanca/MA), M. R. Ahmad Rusmili, T. Y. Tee (Selangor/MY), R. Saile, N. Ghalim (Casablanca/MA), I. Othman (Selangor/MY) P187 Striatal global phosphoproteomics analysis of the zQ175 Huntington’s disease mouse model following PDE10A inhibition J. N. Dybowski (Martinsried/DE), V. Beaumont (Los Angeles/US), C. Schaab, K. Grundner-Culemann, S. Elschenbroich (Martinsried/DE), D. Lavery (Los Angeles/US), C. Schmidt (Cambridge/US), I. Munoz-San Juan (Los Angeles/US) P188 Clinical interest of human intestinal mucus proteomics P.-A. Gómez Buitrago, C.-A. Gonzalez-Correa, G. Taborda-Ocampo, R. Acuña-Sornoza, M. Santacoloma-Osorio (Manizales/CO), M.-A. Zezzi-Arruda (Campinas/BR) P189 H2S role in amyotrophic lateral sclerosis: unravelling new cellular and molecular mechanisms V. Greco, A. Spalloni (Rome/IT), C. Piras (Milan/IT), N. B. Mercuri (Rome/IT), P. Roncada (Milan/IT), P. Longone, A. Urbani (Rome/IT)

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Poster Sessions

P190 Survival and proliferation of acute myeloid leukemia cells is triggered by FLT3-ITD and TLR9 activating different signaling pathways via Bruton’s tyrosine kinase J. Corso (Göttingen/DE), T. Oellerich (Frankfurt a. M., Heidelberg/DE), S. Mohr, C. Doebele, S. Münch, G. Bug (Frankfurt a. M./DE), J. Beck (San Jose/US), J. Wicht (Heidelberg/DE), M. Oellerich (Frankfurt a. M./DE), H. Bohnenberger, C. Perske (Göttingen/DE), E. Schütz (San Jose,/US), H. Serve (Frankfurt a. M., Heidelberg/DE), H. Urlaub (Göttingen/DE) Quantitative Proteomics II P216 Quantitative mass spectrometric analysis reveals the target acetylome of Epigenetic Inhibitor UVI5008 V. Sharma, A. de Lera, E. Lasonder, H. G. Stunnenberg (Plymouth/UK) P217 Identification of a novel neurotrophic factor from primary retinal Müller cells using SILAC C. von Törne, J. Menzler, A. Ly, N. Senninger (Neuherberg/DE), M. Ueffing (Tübingen/DE), S. Hauck (Neuherberg/DE) P218 Using study design to streamline data processing of highly multiplexed quantitative experiments M. Scigelova, T. N. Arrey, E. Damoc (Bremen/DE), R. Viner (San Jose/US), B. Delanghe (Bremen/DE) P219 Relative quantitation of N-linked glycans using carbonyl-reactive Tandem Mass Tag™ (TMT™) reagents J. Saba, M. Oppermann, P. Suemchen (Stockholm/SE) P220 Quantifying safety protein biomarkers for drug induced organ injury in animal models and humans B. H. J. van den Berg, H. Planatscher, F. Weiss, H. Hammer, T. O. Joos, O. Pötz (Reutlingen/DE) P221 Quantification of palmitoylated proteins in T cells using acyl-biotin-exchange E. Morrison, B. Kuropka, S. Kliche, E. Krause, C. Freund (Berlin/DE) P222 MSQBAT: a platform-independent software suite for the label-free quantification of proteins from (Ge)LC-MALDI-MS and (Ge)LC-ESI-MS data A. Kerner, W. Nadler, C. Rösli (Heidelberg/DE) P223 Phosphoserine-mediated protein interactions of the T cell adaptor protein ADAP A. Manns, E. Krause, C. Freund (Berlin/DE)

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Poster Sessions


P224 Oyster shotgun proteomics provide new insights into various cellular pathways of multiple climate-related stressors D. Ramadoss, V. Thiyagarajan (Hong Kong/CN) P225 Global protein oxidation profiling of Podospora anserina mitochondria suggests efficient removal of damaged proteins even at high age C. Ramallo Guevara (Bochum/DE), O. Philipp, A. Hamann, H. D. Osiewacz (Frankfurt a. M./DE), S. Rexroth, M. Rögner, A. Poetsch (Bochum/DE) P226 High resolution quadrupole TOF for quantitative proteomics S. Beck, F. Meier (Martinsried/DE), A. Michalski, M. Lubeck, S. Kaspar, O. Raether (Bremen/DE), I. Paron, J. Cox, M. Mann (Martinsried/DE) P227 pyQms – a versatile and fast python module for accurate quantitative mass spectrometry J. Barth, A. Niehues, M. Hippler, C. Fufezan (Münster/DE) P228 In vivo cell type specific analysis of protein-protein interactions D. Vasiljevic, S. Seelk, B. Tursun, M. Selbach (Berlin/DE) P229 Development of a gel-based quantitative mass spectrometry assay for determining the fragmentation of cardiac troponin T in serum A. Streng, D. de Boer, F. Bouwman, E. Mariman, M. van Dieijen-Visser, W. Wodzig (Maastricht/NL) P230 specL – An R/Bioconductor package to prepare peptide spectrum matches for use in targeted proteomics C. Panse, J. Grossmann, C. Trachsel, R. Schlapbach (Zurich/CH) P231 High resolution proteomics C. Koehler (Oslo/NO) P232 Multilayered Genetic and Omics Dissection – a new age for biomedical researches Y. Wu (Zurich/CH), E. Williams, J. Auwerx (Lausanne/CH), R. Aebersold (Zurich/CH) Structural Proteomics P233 FtsZ protofilaments use a hinge-opening mechanism for constrictive force generation Y. Li (Hangzhou, Zhejiang Province/CN)

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Poster Sessions

P234 Quantitative XL-MS and different crosslinker reveal parallel orientation of HOP2 and MND1 E. Rampler, T. Stranzl, Z. Orban-Nemeth, D. Hollenstein, O. Hudecz, P. Schloegelhofer, K. Mechtler (Vienna/AT) P235 Enrichment of cross-linked peptides using charge-based fractional diagonal chromatography V. Tinnefeld, S. Verhelst, R. P. Zahedi, A. Sickmann (Dortmund/DE) P236 CASP 11: a blind study towards CLMS as a structure determination tool M. Mendes (Berlin/DE), A. Belsom (Edinburgh/UK), M. Schneider (Berlin/DE), L. Fischer (Edinburgh/UK), O. Brock (Berlin/DE), J. Rappsilber (Berlin, Edinburgh/DE, UK) P237 Protein structure determination in biological matrices using 3D proteomics and computational biology A. Belsom (Edinburgh/UK), M. Schneider (Berlin/DE), L. Fischer (Edinburgh/UK), O. Brock (Berlin/DE), J. Rappsilber (Berlin, Edinburgh/DE, UK) P238 Quantitative cross-linking/mass spectrometry illuminates protein conformational changes that drive activation of the complement Z. Chen, L. Fischer (Edinburgh/UK), J. Cox (Martinsried/DE), S. Tahir, J.-C. Bukowski-Wills, P. Barlow (Edinburgh/UK), J. Rappsilber (Berlin, Edinburgh/DE, UK) Subcellular Proteomics P239 Hitting the target: novel reagents for the chemical-proteomics based identification of vascular accessible biomarkers S. Hanke, A. Kerner (Heidelberg/DE), Y. Zhang (Dresden/DE), C. Rösli (Heidelberg/DE) P240 Membrane microdomain dynamics in P. falciparum infected erythrocytes: proteome, lipidome and interactome F. Fratini, G. Sferra, C. Ferreri (Bologna/IT), M. Ponzi, E. Pizzi (Rome/IT) P241 Reshaping of the autophagosomal proteome by influenza A virus J. Dengjel, A. Becker, C. Muenz, M. Gannage (Freiburg/DE) P242 Identification of six cell surface proteins for specific liver targeting S. Kux van Geijtenbeek, P. Cutler, S. Simon, A. Ducret (Basel/CH) P243 Secretome and proteome analysis of monocyte-derived dendritic cells stimulated with the major birch pollen allergen Bet v 1 L. Strasser, C. Ramsauer, J. Horejs-Hoeck, C. Huber (Salzburg/AT)

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Poster Sessions


P244 A plasma membrane proteomic analysis of mouse and human cardiovascular proteins A. Gramolini (Toronto/CA) P245 Protein determination in RNAlater fixed cells returned from the ISS J. Pietsch, S. Riwaldt (Magdeburg/DE), A. Sickmann (Dortmund/DE), J. Segerer, A. Schwarzwälder, C. Bruderrek (Friedrichshafen/DE), J. W. Bauer (Martinsried/DE), M. Wehland-von Trebra, M. Infanger (Magdeburg/DE), D. Grimm (Magdeburg, Arhus/DE, DK) P246 Functional plasticity of cardiac interfibrillary mitochondria (IFM) as response mechanism to stress J. Heidler, I. Wittig (Frankfurt a. M./DE), D. Henze (Halle, Saale/DE), C. Krüger (Bad Vilbel/DE), S. Kostin, T. Braun (Bad Nauheim/DE), M. Szibor (Helsinki/FI) P247 Redox complexome profiling: a method to determine the redox dependent dynamics of protein-protein interactions in a whole organelle. I. Wittig, E. Bonke, L. Bleier, F. Richter (Frankfurt a. M./DE), U. Brandt (Frankfurt a. M., Nijmegen/DE, NL), S. Dröse (Frankfurt a. M./DE) P248 Hydrazide-based protein-centered glycoproteomics for the analysis of LPS-mediated membrane protein expression changes in THP-1 cells M. Müller, R. Lehmann, T. Conrad, D. Driesch, H. Slevogt (Jena/DE) P249 The value of subcellular fractionation in proteogenomics studies P. Díez, M. Gonzalez-Muñoz, N. Ibarrola, R. M. Degano, C. Drosten, A. Orfao, J. De Las Rivas, M. Fuentes (Salamanca/ES) P250 Proteomics of alternatively activated macrophages reveals novel signalling pathway downstream of scavenger receptor A M. Guo, M. Gierliński, A. Prescott, B. Dill (Dundee/UK), D. Russell (Ithaca/US), M. Trost (Dundee/UK) P251 A large-scale approach to study mitochondrial protein complexes of Arabidopsis thaliana S. Martinez Jaime, M. Górka, A. Graf (Potsdam/DE)


Lecture Abstracts* DGPF Young Investigator Award Tuesday, 24 March 2015: 16:30 –18:00

*76 Participants took part in the competition – 6 are invited for the Young Investigator Award Session

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Lecture Abstracts of Young Investigator Award Session

Lecture Abstracts of Young Investigator Award GRP78 phosphorylation dynamics during sperm maturation V. Lobo1, P. Rao1, R. Gajbhiye1, V. Kulkarni1, P. Parte1 1 National Institute for Research in Reproductive Health (Mumbai/IN)

YIA1

Question: Glucose regulated protein 78 (GRP78), an ‘ER resident chaperone’ plays an important role in protein folding and degradation of misfolded or unfolded proteins. As spermatozoa lack a recognizable ER, GRP78 presence in mature spermatozoa and its relevance to sperm function is an enigma. Previous work by our lab has revealed reduced GRP78 phosphorylation in asthenozoospermatozoa. The present study proposes to investigate in-depth this differential phosphorylation of GRP78. Methods: Differential phosphorylation of GRP78 in asthenozoospermatozoa was determined by Differential in Gel Electrophoresis (DIGE). The presence of GRP78 in rat and human spermatozoa was studied by Western blot (WB) analysis and its localization by Indirect Immunofluorescence (IIF). Enzyme linked Immunosorbent assay (ELISA) was optimized to determine surface localization of GRP78 in intact rat spermatozoa. Immunoprecipitation (IP) experiments were done to determine the type of amino acid residues phosphorylated in GRP78. The extent of GRP78 phosphorylation in rat mature- and immature sperm and its differential phosphorylation in asthenozoospermatozoa vis a vis normal spermatozoa was discerned by Nanofluidic proteomic immunoassay (NIA) and Phosphatase assays. Results: Using Differential in Gel Electrophoresis (DIGE) phosphorylated GRP78 was observed to be > 2 fold less in asthenozoosperm vis-à-vis normozoosperm. WB showed presence of GRP78 in rat testis and spermatozoa as well as in human spermatozoa. IIF indicated surface localization of GRP78 on the equatorial region in case of rat- and in equatorial, midpiece and neck region in case of human- spermatozoa. Post permeabilization it was observed in the neck and ventrally at tip of sperm head for rat and in midpiece of human spermatozoa. Surface localization of GRP78 was also validated by ELISA. GRP78 was observed to be phosphorylated at serine, threonine and tyrosine residues by IP studies. NIA coupled with Phosphatase assays revealed three phosphoforms GP4.96, GP4.94 and GP4.85 in mature rat spermatozoa versus two phosphoforms GP4.96 and GP4.94 in testicular spermatozoa. In human spermatozoa, 3 phosphoforms GP5.04, GP4.96 and GP4.94 were observed, of which, GP4.94 and GP5.04 were significantly reduced in asthenozoospermatozoa. Conclusions: Our study provides novel information on the spatial distribution of phosphorylated GRP78 during sperm maturation. Based on our observations we have proposed a schematic demonstrating the putative dynamic changes in GRP78 phosphorylation in spermatozoa during its maturation. The observation of reduced phosphorylation of GRP78 in the asthenozoosperm and identification of the phosphoforms reduced, suggests relevance of GRP78 phosphorylation to sperm function.

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Exposure of Mycobacterium tuberculosis to thioridazine alters YIA2 the cell envelope permeability; an alternative mechanism for the synergistic effects with other antibiotics J. de Keijzer1, P. de Haas2, A. de Ru1, E. Heerkens2, L. Amaral3, D. van Soolingen2, P. van Veelen1 1 Leiden University Medical Centre (Leiden/NL) 2 National Institute for Public Health and the Environment (RIVM) (Bilthoven/NL) 3 Universidade Nova de Lisboa (Lisboa/PT) The emergence of multidrug resistant tuberculosis mandates the search for alternative drugs. Several neuroleptics, such as the phenothiazine thioridazine, exhibit in vitro activity against Mycobacterium tuberculosis. Thioridazine has already been successfully used in combination with antibiotics for compassionate therapy of patients presenting with extensively drug resistant infections of tuberculosis. Using an unbiased proteomic approach, we set out to unravel the molecular mechanism of this potential new anti-tuberculosis component by examining the impact of continuous thioridazine exposure on the proteome of M. tuberculosis. We discovered that under the influence of thioridazine several proteins involved in the maintenance of the cell wall permeability barrier are differentially regulated. By assessing accumulation of fluorescent dyes in M. tuberculosis over time, we demonstrated that long-term thioridazine exposure of M. tuberculosis affected the mycobacterial cell envelope and increased the permeability towards both hydrophilic and hydrophobic fluorescent compounds, thereby the enhancing the uptake of compounds. Furthermore, we demonstrated that treatment of M. tuberculosis with thioridazine altered the composition of the plasma membrane. These observations may explain the previously reported synergistic effects between thioridazine and other anti-TB drugs. The knowledge of thioridazines mode of action is a major step forward and could facilitate further development of this class of drugs for therapy of multidrug resistant pulmonary infections of tuberculosis. In fact, due to the increased permeability of the cell envelope to old drugs and new anti-tuberculosis antibiotics, not only would the old drug(s) be restored to an effective therapeutic level, the dose levels of new but toxic anti-tubercular compounds may be significantly reduced. Improving proteotypic peptide prediction using information retrieval based measures A. Argentini1,2, L. Martens1,2 1 VIB , Department of Medical Proteins Research (Gent/BE) 2 Ghent University, Department of Biochemistry (Gent/BE)

YIA3

Introduction: The prediction of proteotypic peptides is a relevant problem in targeted proteomics, where the right choice of the selected peptide for the proteins of interest improves the quality of the result. Several Machine Learning based predictors for proteotypic peptides exist, and these can be divided into two main groups: (I) the problem is solved using binary classifiers (Fusaro et al. 2009)-(Eyers et al. 2011), or (II) a ranking function is learned from the data (learn-to-rank methods) (Qeli et al. 2014). Objectives: The work described here focused on using an intermediate approach that combines the easy interpretability of the binary classifier approach with the extra information obtained

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from ranking all peptides for a given protein. Moreover, our approach avoids the necessity of specifying an a priori relevance for each peptide in typical learn-to-rank methods (Qeli et al. 2014). Our approach is shown to outperform the best binary classifiers currently available. Material & Methods: The CPTAC dataset (Paulovich et al. 2010) of 43 MS experiments from 5 yeast samples with different concentrations of spiked-in UPS proteins was used. MS1 apex peak intensities were extracted using an in-house tool. 569 sequence-based features (aa composition, aaIndex (Kawashima et al. 1999)) were calculated for each peptide. As training and test sets we have used the yeast and the UPS spiked proteins, respectively. Cross validation was used during training, and the average precision for the 2nd and the 5th position (P@2 and P@5) of the ranked list of peptides on the test set. As predictor we have trained both a fast linear SVM, and a Random Forest regression model that predicts peptide intensity. Results: Our predictor based on a linear SVM outperforms all competitors evaluated both in terms of P@2 and P@5 (Figure 1). The regression model shows better results with respect to the competitors but does not outperform the linear SVM. The optimization of the evaluated classifiers using P@2 and P@5 in the cross-validation thus allows our approach to obtain a well ranked list of the best ionized peptides. Conclusions: In this work we showed that the use of apex peak intensities is really effective to improve the prediction of proteotypic peptides. Moreover, we highlight that addressing the problem as a ranking problem instead of a classification provides a more reliable, more useful prediction, in which candidate peptides for SRM are reported in a ranked order of proteotypicity.

Figure 1 The P@2 and P@5 (average precision at the 2nd and the 5th position of the ranked list of peptides) result of our predictors and the best binary classifiers available (ESP, CONSeQuence) on the test set. Our linear SVM outperforms all the competitors in both the metrics evaluated.



Label-free quantification of GeLC-MALDI data with a novel software reveals YIA4 a subtype-specific protein signature for pancreatic ductal adenocarcinoma W. Nadler1,2, A. Kerner1,2, B. Klaus1,2,3, L. Kuhlmann1,2, C. Rösli1,2 1 German Cancer Research Center (Heidelberg/DE) 2 Heidelberg Institute for Stem Cell Technology (Heidelberg/DE) 3 European Molecular Biology Lab, Centre for Statistical Data Analysis (Heidelberg/DE) Introduction: In 2011, Collisson and co-workers defined three different subtypes (classical, exocrine-like and quasi-mesenchymal) of pancreatic ductal adenocarcinoma (PDAC) based on a gene signature study using microdissected human tumors. Patients with distinct subtypes showed significant differences in clinical outcome and therapeutic response (Collisson et al., Nat Med. 2011). A proteomic signature of the different subtypes could yield biomarkers for diagnosis and targeted therapies. Objectives: In order to identify biomarkers for cancer diagnosis and patient stratification, 12 primary human PDAC cell lines from 9 different patients were proteomically characterized with a label-free GeLC-MALDI approach. The novel software suite MSQBAT was developed to quantify the GeLC-MALDI data and results were evaluated using multiple reaction monitoring of biological markers and GeLC spike-in test sets. Material & Methods: The proteome of 12 patient-matched PDAC cell lines (grown under serum-free conditions) and 2 control cell lines were analyzed in a GeLC-based bottom-up approach (Figure 1). Peptides from each of the 12 gel fractions per cell line were separated into 600 fractions with a 110 min gradient (11-40% ACN, 0.1% TFA). The fractions were spotted on a MALDI target plate using a SunChrom robot and analysed with a MALDI TOF/ TOF™ 5800 mass spectrometer (AB SCIEX). Peak lists (Data Explorer) and identifications (ProteinPilot™, both AB SCIEX) of proteotypic peptides (PepSir, build in-house) were loaded into MSQBAT and the gel sample sets were quantified with respect to a super-alignment of all cell lines. Results: A total of 5627 proteins were identified based on proteotypic peptides and 4492 of these were quantified within all 14 cell lines. Unsupervised clustering robustly separated the exocrine subtype and the controls from the other cell lines. Comparison of the proteomic data with data from an Illumina® Gene Expression Array showed highly consistent results for clusters of regulated gene families. Within the exocrine subtype proteins involved in biotransformation, e.g. members of the CYP450 and AKR families were significantly up-regulated (Figure 2). Several markers were validated with multiple reaction monitoring, confocal laser scanning microscopy and classical proteomics techniques. Using the resulting MRMs, the performance of the MSQBAT software was evaluated. Despite different sample preparation techniques (quantification along 12 gel slices for MSQBAT or shotgun for MRM quantification), both methods yielded a highly consistent and stable quantification. Conclusions: MSQBAT, a novel software suite, enables the label-free quantification of GeLCbased MALDI data, resulting in the identification of a subtype-specific protein signature for PDAC and the accession of regulated protein families with potential clinical relevance.

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Figure 1

Figure 2




Proteomic analysis of barley cell nuclei YIA5 I. Chamrád1, J. Urinovska1, R. Lenobel1, H. Jerabkova2, B. Petrovska2, M. Sebela1 1 Centre of the Region Haná for Biotechnological and Agricultural Research, Palacky University, Department of Protein Biochemistry and Proteomics (Olomouc/CZ) 2 Institute of Experimental Botany AS CR, Department of Proteomics of Cell Structures, (Olomouc/CZ) Introduction: In eukaryotes, a majority of the hereditary information is localized in the nucleus. Therefore, almost all DNA-related processes occur in this organelle, including DNA repair, replication, recombination, and transcription as well as appropriate regulatory circuits. These important biological phenomena cannot be completely understood without a secure characterization of the nuclear proteins. Given the (potentially) high number of individual protein species to be analyzed, modern high-throughput proteomic approaches are ideally suitable for comprehensive study of nuclear proteomes. Indeed, many works have been published on proteomic analysis of animal nuclei over the past decade. On the other hand, only sparse proteomic reports have dealt with nuclear proteins from plants. This clear imbalance is presumably due to troublesome sample preparation caused by high integrity of plant cells, presence of many interfering secondary metabolites and high endogenous protease activity. To shed new light on “the protein dark matter” of plant nuclei a protocol comprising flow cytometry sorting of formaldehyde-fixed nuclei coupled to protein/peptide separation and MS analysis was proposed. Hereby, preliminary results from its application to a study of barley cell nuclei in G2 phase are presented. Objectives: The main objective of this study was to test the compatibility of plant nuclei flow cytometry sorting and subsequent proteomic analysis of nuclear proteins. Materials and methods: The methodology involved the following steps: barley seedling cultivation, formaldehyde fixation, homogenization of seedling root tips, preparation of crude nuclei and their flow cytometry sorting, protein extraction, SDS-PAGE, in-gel digestion of proteins, nanoLCESI-MS and nanoLC-MALDI-MS and bioinformatic analysis of acquired data. Results: Using the proposed protocol, over 2000 proteins were identified covering three orders of protein abundances. A GO term analysis confirmed that the vast majority of the proteins were nucleus-related with the most enriched processes connected to chromatin assembly and organization, ribosome biogenesis and RNA processing. To our knowledge, this dataset represents the largest complex of plant nuclear proteins identified in a single proteomic study so far. Conclusion: Successful identification of thousands of proteins indicates that the presented protocol has several advantages. Firstly, the results suggest that fast and simple sample preparation combined with formaldehyde fixation helps to preserve the nuclear proteome from unwanted artificial changes (protein degradation etc.). Secondly, flow cytometry allows the separation of G1, S and G2 phase nuclei and provides fractions with high purity. Thirdly, the protocol can be easily extended to any type of plant tissue. Fourthly, as the intact plant chromosomes can be readily isolated employing the same flow sorting procedure, the protocol can be adapted for the study of chromatin-associated proteins. Altogether, it seems that coupling flow cytometry with proteomic techniques provides an elegant and powerful means for mapping of the plant nuclear proteome.

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Proteomic evaluation of nucleocytoplasmic partitioning YIA6 and the CRM1-dependent nuclear exportome S. Karaca1, K. Kirli2, D. Görlich2, H. Urlaub1,2 1 MPI for Biophysical Chemistry, Bioanalytical Mass Spectrometry Group (Göttingen/DE) 2 University Medical Center Goettingen, Department of Clinical Chemistry (Göttingen/DE) Question: The defining eukaryotic organelle is the nucleus, which is separated from cytoplasm by the nuclear envelope (NE). Passive diffusion and importin- as well as exportin-mediated active nuclear transport through nuclear pore complex determine how proteins partition between the cytoplasm and the nucleus. Despite the importance of this partition in eukaryotic life, it has not been thoroughly characterized due to the challenges to obtain cytoplasmic and nuclear proteomes without cross-contamination. Therefore, we worked with a model system (X. leavis oocytes) that allows manual micro-dissection to obtain cytoplasmic and nuclear proteomes with unmatched purity. We futher employed CRM1/Exportin-1 affinity chromatography (CAC) to identify cargo spectrum of most promiscuous exportin. Methods: Enucleation of X. laevis oocyte: Oocytes were manually micro-dissected with fine forceps to obtain cytoplasmic and nuclear proteomes. Universal Proteomics Standard 2 was spiked into cytoplasmic and nuclear extract to estimate absolute protein concentration. CRM1/Exportin-1 affinity chromatography: CRM1 immobilized beads were incubated with X. leavis extract in the absence or presence of RanQ69L. LC-MS/MS and data analysis: X. leavis cytoplasmic and nuclear extracts and eluted proteins from CRM1 affinity chromatography were separated by SDS-PAGE and in-gel digestied. Extracted peptides were analyzed with Agilent LC system coupled to either LTQ-Orbitrap Velos or Q-Exactive (both Thermo Scientific) as triplicates. Raw MS data were analyzed with MaxQuant (1) and were searched against X. leavis protein database (2). Results: Here, we quantified the nucleocytoplasmic distribution of ~7500 proteins: ~2500 of these showed an exclusive nuclear, ~2000 an exclusive cytoplasmic and the rest an intermediate localization (Figure 1.C). These numbers have interesting implications e.g. for RNA metabolism, protein degradation, and organization of the cytoskeleton. CRM1 affinity chromatography led to identification of hundreds of potential CRM1 export cargoes (Figure 2.C). Majority of the potential CRM1 cargoes relate to cytoplasmic activities that should be (at least temporarily) suppressed inside nuclei. These include nearly all translation factors, certain cytoskeletal proteins and vesicle coat proteins. Conclusions: We present a valuable resource for the nucleocytoplasmic distribution of Xenopus leavis proteins, provide insights into eukaryotic compartmentation, and dramatically expand the known CRM1 cargo repertoire. References: 1 Cox, J. & Mann, M., Nat. Biotechnol., 26, 1367–1372 (2008). 2 Wühr, M. et al., Curr. Biol. 24, 1467–1475 (2014).



Figure 1 A. Workflow of spatial proteomics of X. leavis oocyte. B. SDS-PAGE analysis of cytosolic and nuclear extracts. C. Venn diagram shows protein identificaiton comparison between cytoplasmic and nuclear proteomes.

Figure 2 A. Workflow of CRM1 affinity chromatography (CAC). B. SDS-PAGE analysis of eluted proteins with and without RanQ69L incubation. C. Scatterplot represents label free quantification of CAC. Blue-colored dots represent potential CRM1 cargoes.

ProkaGENOMICS 2015 6th European Conference on Prokaryotic and Fungal Genomics

CALL FOR ABSTRACTS

29 September–2 October 2015

GÖTTINGEN • GERMANY

ABSTRACT DEADLINE: 20 APRIL 2015 www.prokagenomics.org

General Information


Poster Sessions The poster sessions take place Sunday (poster session A), Monday (poster session B) and Tuesday (poster session C) and all posters are assigned to these sessions according to the scheme below. The poster exhibition is located on the ground floor, first floor and in the historic “Lichthof”, the Atrium at the Technical University Berlin. For finding your exact poster position, please have a look at the poster plan onsite and look for your abstract category. Please follow the signage onsite. The presenting authors should be at their poster during the corresponding poster session and award session in order to participate in the competition. Poster Session A Sunday, 22 March 2015, 19:00–20:30 hrs Bioinformatics Free Topics Proteomics for Human Health I Proteomics in Biotechnology Quantitative Proteomics I

P014–P031 (see p. 29 ff.) P032–P049 (see p. 30 ff.) P123–P145 (see p. 32 ff.) P192–P198 (see p. 34 ff.) P199–P215 (see p. 35 ff.)

Foyer ground floor Foyer ground floor Atrium Atrium Atrium


Foyer ground floor Foyer ground floor Foyer first floor Foyer first floor Foyer first floor Atrium


Foyer first floor Foyer first floor Atrium Atrium Atrium Atrium

Poster Session B Monday, 23 March 2015, 15:00–16:30 hrs Affinity Proteomics Imaging Mass Spectrometry Technologies Plant and Microbial Proteomics I Post-Translational Modifcations I Proteomics for Human Health II Poster Session C Tuesday, 24 March 2015, 15:00–16:30 hrs Plant and Microbial Proteomics II Post-Translational Modifcations II Proteomics for Human Health III Quantitative Proteomics II Structural Proteomics Subcellular Proteomics

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General Information

Registration Fees Full congress pass Day ticket (Monday–Wednesday) Member DGPF or EuPA* 500 EUR 200 EUR Non-Member 600 EUR 250 EUR Member, Student 300 EUR 100 EUR Non-Member, Student 400 EUR 150 EUR Congress Dinner 40 EUR The participation in the pre-Workshops on Sunday is free, except the Educational Day Session, which will be charged at the day ticket fares. * Please have your EuPA membership number at hand. General Terms and Conditions Please find our General Terms and Conditions at www.proteomic-forum.de Congress Language The official congress language is English. Instructions for Speakers To ensure the conference runs smoothly and fairly for all participants, please do not allow your presentation to run overtime. The chairs are instructed to strictly monitor the time and to interrupt the presentation if the speaking time is exceeded. The only multimedia technology accepted is MS-PowerPoint. A presentation notebook with a PDF reader and MS Office PowerPoint 2010/2007 will be provided. Using your own laptop is not recommended. A notebook, presenter and laser pointer are available at the speakers’ podium in the lecture hall. A technical supervisor can help you. Please note: Certain encodings for video and audio files could lead to problems. Please visit our speakers’ ready room. Should you wish to use non-digital equipment, please contact us. We can be reached at [email protected]. Speakers’ Ready Room All presenters are requested to submit their presentation at least two hours before the start of their session to the media-check. If your session is in the morning, please submit your presentation the preceding evening. The speakers’ ready room is located on the first floor in Room 1036 (see opening hours p. 70). For submission of your presentation, please use a USB flash drive, CD or DVD disc and that should not be electronically protected with software. You may view and/or edit your presentation. The media check for the pre-Workshops and the News Corner Session on Sunday, 22 March 2015 will be in the respective room.

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General Information


Requirements for Poster Posters should be printed no larger than DIN A0 (84.1 cm x 118.9 cm) and not laminated. Poster pinboards are 120 cm x 150 cm and are equipped with pins and a poster number. The mounting material will be supplied on site. No other material should be used. The posters should be mounted on the respective day until 9:00 hrs, except Sunday until 17:00 hrs and must be removed by Tuesday, 24 March 2015 until 17:00 hrs. Please note that all posters which have not been removed cannot be saved or sent back to the authors. Abstracts All abstracts are provided within the abstract book that can be downloaded online at www.proteomic-forum.de. Awards and Prizes Poster Prizes of the German Society for Proteome Research First Prize 1.000 EUR Second Prize 500 EUR The evaluation will take place on site by an independent poster committee. Poster prize candidates will be informed about the status of their poster on site and are kindly asked to attend the award ceremony on Wednesday, 25 March 2015 from 16:30-16:45 hrs. Young Investigator Award Scientists participating at the Proteomic Forum 2015 and being younger than 33 years are invited to apply for this year´s Young Investigator Award of the German Society for Proteome Research. There are 3 prizes: 1.000, 500 and 250 Euro. In addition: all selected presenters are invited to take part in the Speaker’s Dinner on top of the German Reichstag. A senior scientist evaluation committee will invited 6 selected applicants to present their results during the YIA Session on Tuesday, 24 March 2015, 16:30–18:00 hrs. The winners are determined by voting directly by the audience after the last presentation. Promotion Prize The promotion of young scientists is one of the main tasks of the German Society for proteome research (DGPF e.V.). For this purpose, the DGPF has inaugurated the Promotion Prize donated with 2.500 EUR which will be awarded for the first time to an outstanding dissertation in the field of proteomics which was finished during the last 2 years. Here, the originality as well as the duration of work and a conceivably high degree of independency will be the basis for awarding the Promotion Prize. All winners will be announced at the Award Ceremony on Wednesday, 25 March 2015, 16:30–16:45 hrs.

70

General Information

Opening Hours

Industrial Exhibition Poster Exhibition Check-In Speakers’ Ready Room Internet Lounge Cloakroom

Sunday 15:30–21:00 17:00–21:00 08:00–19:00 16:00–19:00 16:00–19:00 08:00–21:00

Monday 10:30–16:30 08:00–18:00 08:00–18:00 08:00–18:00 08:00–18:00 08:00–19:00

Tuesday 10:30–16:30 08:00–18:00 08:00–18:00 08:00–18:00 08:00–18:00 08:00–18:30

Wednesday 10:30–15:30 08:00–15:30 08:00–17:00 08:00–15:00 08:00–15:00 08:00–18:00

Internet Wireless internet will be available in the Internet lounge in Room 1036 or with your own laptop. An individual WiFi-code will be handed out at the Check-In. Travel Public transport stops near the main campus Underground: Ernst-Reuter-Platz, Line 2 (red line) Bus: Ernst-Reuter-Platz, Lines M45, 245 and X9 Steinplatz, Lines M45 and 245 S-Bahn: Zoologischer Garten, Lines S5, S7, S75 Tiergarten, Lines S5, S7, S75 By car Please have a look at the congress website concerning the arrival description. Parking There are several parking areas along the „Straße des 17. Juni“ number 110–114, just next to the Ernst-Reuter-Haus, as well as in the Bachstraße at the S-Bhf. Tiergarten. By train or plane Please have a look at the congress website concerning travel cooperations with German Railways and for description for arriving at airport Berlin Tegel or Schönefeld and how to get to the congress venue. Acting today for tomorrow: Travel by train from Euro 99 with 100% green power to the CONGRESS Acting today for tomorrow: Travel by train from Euro 99 with 100% green power to the Proteomic Forum 2015! To book call +49 (0)180 6 31 11 53** and quote ”CONVENTUS” as reference. Have your credit card ready please.

71

Social Program


Get-Together and Poster Session A • Sunday, 22 March 2015 Join the Get-Together and meet all exhibitors and supporters of the Proteomic Forum 2015 within the industrial and poster exhibition area in the foyers of the ground and first floor and the Atrium of the Technical University in Berlin. All participants are cordially invited free of charge. Date

Sunday, 22 March 2015

Time

19:00–20:30 hrs

Place

Industrial exhibition

Fee

free of charge

Congress Dinner • Tuesday, 24 March, 2015 The restaurant “Nolle” is located in the very heart of Berlin, directly under the historic S-Bahn railway arches at Friedrichstrasse Station. The restaurant, named after Prussian General Friedrich Graf Kleist who was granted the honor of the additional last name “von Nollendorf” for his outstanding service, offers an impressive and original atmosphere of a typical 1920´s restaurant – marble, mirrors, leather furnishings and Art Deco paintings recreate the atmosphere of former Berlin. It is a place of traditional hospitality, showing Berlin as it was in the past and as it continues to be in the present, giving a perfect ambience for our Congress Dinner. Join us for an unique evening, only a few steps away from the most famous attractions of Berlin! Date

Tuesday, 24 March 2015

Time

20:00 hrs

Place

Restaurant “Nolle”

Fee

40 EUR

Address

Georgenstraße/S-Bahnbogen 203 10117 Berlin/DE

Participants are asked to organize their arrival on their own initiative. The restaurant “Nolle”, next to Station “Friedrichstrasse”, is easy to access by public transport: S-Bahn S1, S2, S25, S5, S7, S75 Subway U6 Tram M1 Bus 147, N6 Train RE1, RE2, RE7, RB14 Connection details are given on the website of the Berliner Verkehrsbetriebe (BVG).

67.

www.dghm-kongress.de

Jahrestagung der Deutschen Gesellschaft für Hygiene und Mikrobiologie (DGHM) e. V.

2015 27.–30. September 2015

Halle Münsterland • Münster

Themenschwerpunkte • Infektion – Toxine, Invasine und Glykosylierung • Krankenhaushygiene und Public Health • NGS für mikrobielle genomische Überwachung und darüber hinaus: eine Technologie für alles • TLR und Inflammasom • Zoonosen (Viren, Bakterien und Parasiten)

© www.muenster.de • Presseamt | © fotolia.com • Jakob Kamender

Wissenschaftliche Leitung Prof. Dr. rer. nat. Dr. h. c. Helge Karch Prof. Dr. med. Georg Peters Westfälische Wilhelms-Universität Münster Universitätsklinikum Münster

Sponsors and Media Cooperation


Sponsors Platinum Sponsor Thermo Fisher Scientific GmbH (Dreieich/DE) Gold Sponsor Waters GmbH (Eschborn/DE) Silver Sponsor Matrix Science Ltd. (London/UK) Bronze Sponsors AB SCIEX Germany GmbH (Darmstadt/DE)

Biognosys AG (Schlieren/CH)

Bruker Daltonik GmbH (Bremen/DE)

Sponsor Young Investigator Awards SERVA Electrophoresis GmbH (Heidelberg/DE) Media Cooperation BIOCOM AG “transcript” BIOCOM AG “European Biotechnology” succidia AG „lab&more“ Wiley-VCH Verlag GmbH & Co. KGaA/GIT Verlag „GIT Labor-Fachzeitschrift“ Wiley-VCH Verlag GmbH & Co. KGaA „Proteomics“

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74

Exhibitors

Exhibitors The following exhibitors are looking forward to welcome you and to present their comprehensive range of innovative products for you: Alphabetical order Company Agilent Technologies (Waldbronn/DE) Bioinformatics Solutions Inc. (Waterloo/CA) Bruker Daltonik GmbH (Bremen/DE)

Booth no. 4 18 6

DECODON GmbH (Greifswald/DE)/ NH Dye AGNOSTICS (Halle, Saale/DE)

20

EURISO-TOP GmbH (Saarbrücken/DE)

15

EuPA (Martinsried/DE)

13

HUPO (Santa Fe/US)

14

Expedeon (Cambridge/UK)

8

HORIBA Jobin Yvon GmbH (Bensheim/DE)

16

JPT Peptide Technologies GmbH (Berlin/DE)

5

KNAUER Wissenschaftliche Geräte GmbH (Berlin/DE)

11

LI-COR Biosciences GmbH (Bad Homburg/DE)

9

Matrix Science Ltd. (London/UK)

3

OmicScouts GmbH (Freising/DE)

21

Promega GmbH (Mannheim/DE)

19

Protein Simple (San Jose/US)

14a

SERVA Electrophoresis GmbH (Heidelberg/DE)

10

SHARP Devices Europe (Hamburg/DE)

12

SunChrom GmbH (Friedrichsdorf/DE)

7

Shimadzu Deutschland GmbH (Duisburg/DE)

17

Thermo Fisher Scientific GmbH (Dreieich/DE)

1

Waters Gmbh (Eschborn/DE)

2

Exhibitors


Numerical order Booth no.

Company

1

Thermo Fisher Scientific GmbH (Dreieich/DE)

2

Waters Gmbh (Eschborn/DE)

3

Matrix Science Ltd. (London/UK)

4

Agilent Technologies (Waldbronn/DE)

5

JPT Peptide Technologies GmbH (Berlin/DE)

6

Bruker Daltonik GmbH (Bremen/DE)

7

SunChrom GmbH (Friedrichsdorf/DE)

8

Expedeon (Cambridge/UK)

9

LI-COR Biosciences GmbH (Bad Homburg/DE)

10

SERVA Electrophoresis GmbH (Heidelberg/DE)

11

KNAUER Wissenschaftliche Geräte GmbH (Berlin/DE)

12

SHARP Devices Europe (Hamburg/DE)

13

EuPA (Martinsried/DE)

14

HUPO (Santa Fe/US)

14a

Protein Simple (San Jose/US)

15

EURISO-TOP GmbH (Saarbrücken/DE)

16

HORIBA Jobin Yvon GmbH (Bensheim/DE)

17

Shimadzu Deutschland GmbH (Duisburg/DE)

18

Bioinformatics Solutions Inc. (Waterloo/CA)

19

Promega GmbH (Mannheim/DE)

20

DECODON GmbH (Greifswald/DE)/ NH Dye AGNOSTICS (Halle, Saale/DE)

21

OmicScouts GmbH (Freising/DE)

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76

Exhibitors Plan

Technical University Berlin Ground Floor Industrial exhibiton Main Entrance

First Floor

14

11 12 13

1

10

2

9 3

8 4

7 5 6

R 0104

Poster exhibition Main Entrance

Straße des 17. Juni

R0112 R0111

R0110

R 0107

Info Point

Cafeteria

Scientific Program

Poster Exhibition

Industrial Exhibition

77

Exhibitors Plan


Technical University Berlin First Floor

14a 15

From ground floor

From ground floor

21 20

Atrium

Check-In

Speaker’s Ready Room

19 18

16 17

R 0104

Catering

Cloakroom

Elevator

78

Author List

Author List A Abbondio, M. Abdollahi, A. Aberger, F. Abouseada, N. Acosta-Martin, A. Acuña-Sornoza, R. Addis, M. F. Aebersold, R. Aerts, J. M. Ahmad Rusmili, M. R. Alberio, T. Albers, C. Alge-Priglinger, C. Allmer, J. Alloggio, I. Alruwaili, J. Altmann, F. Amaral, L. Amirmardfar, R. Anders, U. Anedda, R. Ang, C.-S. Antinori Malaspina, P. Argentini, A. Arner, P. Arrey, T. N. Asplund, A. Assinger, A. Atwal, G. Auer, G. auf dem Keller, U. Auwerx, J. Avramova-Nehmer, J. B Bach, M. Baerenfaller, K. Baginski, S. Baker, A. Baldin, C.

51 50 33 47 48 51 32, 47, 51 25, 40, 53 34 51 36 31 51 30 42 34 49 59 30 44 32 36 48 24, 59 38 52 45 46 29 34, 44 49 53 44

P181 P180 P133 P083 P112 P188 P045, P046, P047, P181, P084, P085, P086 P232, PL23, P056 P141 P186 P204 P039 P184 P027 P079 P145 P115 YIA2 P034 P146 P047 P210 P112 YIA3 P002 P218 P159 P167 P015 P139, P149 P120 P232 P152

31 48 19 31 41

P040 P088 PL09 P035 P068

Author List


Bantscheff, M. Baran, P. Barkovits, K. Barlow, P. Bartel, J. Barth, J. Bauer, J. W. Baumann, C. Bazzichi, L. Beaumont, V. Becher, D. Becker, A. Becker, S. Beck, F. Beck, J. Beck, K.-F. Beck, M. Beck, S. Behne, A. Behr, J. Beine, B. Beissbarth, T. Bekesova, S. Belsom, A. Benama, M. Bendixen, E. Berchem, G. Berger, E. Berger, M. Bernhardt, O. Bern, M. Bertile, F. Beynon, R. Bhangu-Uhlmann, A. Bierczynska-Krzysik, A. Bilan, V. Bindel, F. Biosa, G. Blanc, S. Blankenburg, S. Blasius, B.

16, 45 31 44 54 47 53 33, 55 33, 40 45 51 47 54 34, 44 45 52 43 33 53 30 41 40 46 48 54 30 13, 22 37 43 50 29, 36, 37, 43 49 35 34 37 31 43 44 47 35 43 31

P159, NC9 P042 P153 P238 P081 P227, PL32 P136, P245 P127 P158 P187 P081 P241 P139, P149 P162 P190 P107 P134 P226 P025 P065 P053 P168 P087 P236, P237 P032 PL21 P213 P097 P178 P106, P212, P214, P024 P119 P202 P141 P215 P042 P104 P152 P084 P202 P101, P102 P036

80

Blatnik, R. Bleier, L. Blennow, K. Bleuler, S. Blixt, E. Bloomfield, N. Bocanek, O. Bockmayr, M. Bock, T. Bodenmiller, B. Boege, F. Boese, J.-H. Bogdanow, B. Böhmert, L. Bohnenberger, H. Boldt, K. Bonaldi, T. Bonizzi, L. Bonke, E. Bonn, F. Borchers, C. Bourchookarn, A. Bousquet-Dubouch, M.-P. Bouwman, F. Bouyssie, D. Brabant, G. Braeuning, A. Brakhage, A. A. Brandl, C. Brandt, U. Braun, H.-P. Braunschweig, T. Braun, T. Breitenbach-Koller, H. Breitenbach, M. Brock, O. Bröker, B. Bruch, H.-P. Bruderer, R. Bruderrek, C. Bruley, C. Brummer, T. Bryan, K.

Author List

33 40, 55 36 29 32 40 31 33 33 20 32 45 50 44 52 32, 38, 45 49 35, 42 55 47 12, 25, 27 41 18 53 30 46 44, 45 31, 41 33 40, 55 19, 29, 41 34 55 33 33 54 32 35 29, 36, 37, 43 55 30 44 32

P132 P247, P059 P208 P024 P126 P057 P037 P131 P134 PL11 P124 P159 P176 P155 P190 P004, P160, P125 P121 P079, P195 P247 P081 HUPO07, PL28 P067 O02 P229 P032 P164 P155, P156 P068, P038 P136 P247, P059 P071, P023 P139 P246 P136 P136 P236, P237 P049 P193 P106, P212, P214, P024 P245 P032, O06 P151 P125

81

Author List


Büchler, R. Bug, G. Bukowski-Wills, J.-C. Burch, T. Burel, A. Burkhart, Julia Burlet-Schiltz, O. Busch, D. Busch, T. Butscheid, Y. Büttner, K. Butzmann, L. C Cabanski, M. Capkova, V. Carapito, C. Carrie, C. Cedersund, G. Cermak, J. Chait, B. T. Chamrád, I. Charwart, V. Chávez Munguía, B. Chen, J.-X. Chen, L.-Y. Chen, Z. Chiocca, S. Ciftci, C. Ciprian, P. G. Ciregia, F. Claude, E. Claussnitzer, M. Clement, L. Cloots, E. Collins, A. Collins, B. Combe, C. Conrad, T. Corradini, E. Corrales, F. Corso, J. Cox, J. Croizat, P.

34 52 54 31 30 21 30 35 41 36 47 45

P142, P143 P190 P238 P035 P032

35 42 30 47 45 44 18, 21 24, 38, 63 34 44 39 36 54 49 30 39 45 39, 40 38 26, 29 39 29 40 40 55 18 13 52 18, 53, 54 49

P199 P078 P032 P080 P157 P150 PL18 YIA5, P006 P192 P154 P013 P212 P238 P121 P027 P013 P158 P052, P055 P002 P014, O20 P012 P015 P056 P054 P248 O01

P032 P203 P071 P212 P081 P159

P190 P226, P238 P120

82

Cruz Mireles, N. D. J. Cubeddu, T. Cuccurullo, M. Cutler, P. D Dahlman, I. Damoc, E. Daniels, D. Darula, Z. David, J. de Boer, D. De Ceuninck, L. Degano, R. M. de Haas, P. Deininger, W. de Keijzer, J. Dekker, N. Delanghe, B. De Las Rivas, J. Deleidi, M. de Lera, A. Deligios, M. DellÁica, M. Demianova, Z. Demmers, J. Dengjel, J. Depping, R. Dernayka, L. de Ru, A. De Sutter, D. Deusch, S. Dhople, V. M. Dianes, J. A. Dickhut, C. Diedrich, B. Dietrich, D. Dieu, M. Díez, P. Difilippantonio, M. Dijon, S. Dill, B. Dirk, A.-L. Dirnberger, B.

Author List

47 32, 47 36 54

P082 P045, P046, P084 P204 P242

38 52 50 49 45 53 39 55 59 37 24, 59 34 52 55 38 52 47 48 41 48 44, 54 35 32 59 39 41 32, 40 29 48 44 40 38 55 34 35 55 46 48

P002 P218 P172 P118, P119 P156 P229 P012 P249 YIA2 P215 YIA2 P141 P218 P249 P003 P216 P084, P085 P111 P064 P108 P151, P241 P193 P125 YIA2 P012 P069 P049, P062 P016 P111 P151 P053 P249 P001 P139 P199 P250 P164 P091

83

Author List


Doblmann, J. Doebele, C. Doellinger, J. Dojahn, J. Domon, B. Dorantes Torres, C. V. Dörrbaum, A. Dovichi, Norman J. Drici, L. Driesch, D. Dröse, S. Drosten, C. Ducret, A. Duda, G. N. Dürnberger, G. Dusberger, F. Dybowski, J. N. Dyr, J. E Ebrahimi, M. Eckhard, U. Edwards, I. Egli, F. Ehrenberger, T. Eichacker, L. Eichelbaum, K. Ekkebus, R. Elamin, A. Eliuk, S. El Kadmiri, N. El Magraoui, F. Elschenbroich, S. Emery, Patrick Encarnación Guevara, S. M. End, C. Engelmann, B. Engelmann, S. Ensle, P. Epping, F. Erdmann, J. Ernst, G. Escher, C. Esdar, C.

29 52 38 25, 33, 40, 42 15, 16, 37, 46 47 41 17 26 55 40, 55 55 54 39 41 29 51 44

P022 P190, P213 P005 P127, P056, P057, P094 P169, ED4 P082 P064

49 31 39, 40 49 36 23, 43 50 39 35 17 50 33 51 25 44 50 46 22 44 35 45 39 29, 36 50

P170 P041 P052, P055 P120 P212 P100, O16 P176 P008 P199

O25 P248 P247, P059 P249 P242 P051 P064 P022 P187 P148, P150

P171 P129 P187 P154 P172 P164 O14 P152 P193 P161 P050 P212, P024 P179

84

Estrada Navarrete, G. Eyckerman, S. F F. Medzihradszky, K. Faerber, F. Falkenberg, H. Farnsworth, C. L. Fasano, M. Feenders, C. Ferreri, C. Ferro, M. Fichtenbaum, A. Fiedler, S. Fila, J. Finkemeier, I. Fischer, L. Forbrig, C. Franzén, B. Fratini, F. Fraumene, C. Frese, C. Freund, C. Friauf, E. Fricke, A. L. Friedrich, A. Friedrich, N. Fritzsche, B. Frydman, C. Fuchs, J. Fuchs, S. Fuentes, M. Fufezan, C. Führer, D. Fürst, D. O. Füssl, F. G Gabius, H.-J. Gacek-Matthews, A. Gajbhiye, R. Gallardo-Rincón, D. Gallien, S. Galozzi, S. Gambaryan, S.

Author List

47 39

P082 P012

45 34 49 36 31 54 19 42 40 42 23 30, 54 49 40 44 54 47 18 52 32 49 33 46 35 44 38 31 55 27, 45, 53 46 49 34

P119, P118 P159 P144 P117 P204 P036 P240 O06 P095 P061 P078 O15 P028, P236, P237, P029, P238 P054 P149 P240 P084, P085

40 49 58 34, 44 37 14, 44 45

P061 P115 YIA1 P154, P138 P213 P153 P162

P221, P223 P048 P116 P136 P164 P193 P146 P004 P044 P249 P162, P227, PL32 P164 P116 P137

Author List


Gandhi, T. Gannage, M. Gao, W. Garcia, B. A. Gardner, D. Garibay-Cerdenares, O. L. Garin, J. Garrigues, L. Gasser, T. Gateau, A. Gauquelin-Koch, G. Gavin, A.-C. Gebhardt, C. Geiger, J. Gemoll, T. Gerdle, B. Gerhard, D. Gerhard, R. Gerstmair, A. Gesell Salazar, M. Gessulat, S. Gethings, L. A. Gevaert, K. Ghafouri, B. Ghafouri, N. Ghalim, N. Ghisaura, S. Giacomelli, C. Gielisch, I. Gierliński, M. Gierok, P. Giese, S. Giusti, L. Gloeckner, C. J. Gobom, J. Gocmen, B. Goeminne, L. Goerdayal, S. Gómez Buitrago, P.-A. Gomis-Ruth, F. X. Gonczarowska-Jorge, H. Gonzalez-Correa, C.-A. Gonzalez de Peredo, A.

36, 37, 43 54 36 42 36 44 30 30 38 30 35 21 40 45 34, 35, 44 32 41 45 45 31, 40 45 29, 34, 36 29, 39 32 32 51 32 45 35 55 32 30 45 38 36 31 29 39 51 31 48 51 30

P106, P212, P214 P241 P211 P094 P210 P154 P032 P032 P003 P032 P202 PL15 P058 P162 P139, P140, P149, P193 P126 P071 P161 P159 P044, P062 P159 P015, P204, P206, P141 P014, P012 P126 P126 P186 P046 P158 P201 P250 P049 P028 P158 P003 P208 P043 P014 P008 P188 P041 P111 P188 P032

86

Gonzalez-Muñoz, M. González-Robles, A. Górka, M. Görlich, D. Gorza, M. Goulas, T. Graf, A. Grallert, H. Gramolini, A. Greco, V. Grimm, D. Grinberg, L. T. Griss, J. Gröer, C. Grossmann, J. Gruber, C. Gruhn, F. Gruissem, W. Grundner-Culemann, K. Guaitoli, G. Gu, H. Gunsalus, K. C. Guntinas-Lichius, O. Guo, M. Guo, Y. Gupta Bhattacharya, S. Gustavsson, M. Gyulkhandanyan, A. H Haas, H. Habermann, J. K. Hafidh, S. Hahne, H. Hallström, B. Hamann, A. Hammer, E. Hammer, H. Hanf, B. Hanke, S. Hanke, T. Hansson, G. C. Harms, M. Harris, M.

Author List

55 44 48, 55 64 51 31 48, 55 38 12, 55 35, 42, 51 55 32 29 35 53 49 50 48 51 27 49 39 39 55 35 41 45 30

P249 P154 P251, P089 YIA6 P183 P041 P251, P089 P002 P244 P079, P195, P189 P245 P123 P016 P203 P230 P115 P173 P088 P187 O28 P117 P013 P050 P250 P195 P070 P157 P030

41 34, 35, 44 42 45 45 53 40, 46 52 31 54 33 43 38, 43 33

P068 P139, P140, P193, P149 P078 P159 P159 P225 P164, P062 P220 P038 P239 P130 P097 P099, P007 P134

Author List


Hartwig, S. Has, C. Hauck, S.

35, 44 30 25, 38, 43, 44, 50, 51, 52

Hauner, H. Havanapan, P.-O. Havrdova, L. Heazlewood, J. Heck, A. J. R. Hecker, M. Heemskerk, J. Heerkens, E. Heidari-Keshel, S. Heide, H. Heidler, J. Heinrich, L. Heinsen, H. Heinzlmeir, S. Heldmann, S. Hellman, U. Helm, D. Hempel, K. Hendricks, M. Henkel, C. Hennrich, M. Henze, A. Henze, D. Herber, J. Hermes, O. Hermjakob, H. Hernández Ortíz, M. Hernández-Ramírez, V. Hetzer, M. Heyer, R. Hibti, F. Hildebrandt, P. Hildebrandt, T. Hill, Andrew Hinneburg, H. Hintner, H. Hippler, M. Hochgräfe, F. Hoeng, J.

38 41 30 19 17, 18, 39 47 45 59 49, 51 40 12, 55 35 32 39 39 34 22 35 34 20, 26, 40 16 15 55 33 49 29 44 34, 44 33 30 44 32 41 26 43 33 53 38, 43 35

P149, P193 P027 P002, P147, P184, P105, P217, P173, P183 P002 P067 P033 PL08 P008, PL07 P081 P162 YIA2 P170, P185 P059 P246 P193 P123 P009 P050 P139 P196 P144 P053, O10 NC8 NC4 P246 P135 P120 P016 P154 P154, P138 P134 P025 P146 P049 P071 PL30 P098 P136 P227 P099, P007, P101, P102 P199

88

Hoffmann, F. Hoffmann, M. Hollenstein, D. Holmes, E. Homuth, G. Honys, D. Hopfgartner, G. Hoppe, S. Horejs-Hoeck, J. Horlacher, O. Horn, N. Hottiger, M. O. Huang, H. Huber, C. Hudecz, O. Huesgen, P. F. Hughes, C. Humblot, P. Hunter, C. Hunyadi-Gulyas, E. I Ibarrola, N. Igci, N. Ihmor, P. Imami, K. Imhof, A. Infanger, M. Ingolia, N. Isaac, G. Ivano, N. V. Ivanov, M. J Jabs, W. Jaquinod, M. Jarboui, M. A. Jennings, P. Jerabkova, H. Jilma, B. Jochim, N. Johanning, S. Johansen, E. John-Schuster, G. Jones, A.

Author List

39 30 54 28 46 42 43 33 54 48 45 43 36 17, 33, 34, 42, 54 54 31 36 35 33, 40, 42 49

P050 P025 P234 PL34 P164 P078 P104 P132 P243 P112 P160 P104 P211 P096, P137, P243, P133 P234 P041 P206 P195 P127, P056, P057, P094 P118, P119

55 31 48 49 25 55 33 31 35 27

P249 P043 P088 P114

31 30 19, 32 50 63 46 45 44 42 50 29

P039 P032 O07, P125 P177 YIA5 P167 P161 P149 P094 P173 P015

P245 P134 P035 P199 O26

89

Author List


Jones, E. Joos, T. O. Jörnvall, H. Jozefowicz, A. Juling, S. Junemann, J. Just, I. K Kalfalah, F. Käll, Lukas Karaca, S. Karl, T. Kaspar, S. Kasper, C. Keck, T. Kelleher, N. Kellner, R. Kempa, S. Kennedy, S. Kepes, F. Kerner, A. Khristenko, N. Kim, Y. J. Kipping, M. Kirkland, T. Kirli, K. Kiseleva, O. Kislinger, T. Kizhakkedathu, J. Klauck, S. Klaus, B. Klauschen, F. Klausegger, A. Kleifeld, O. Klein, O. Klement, E. Klempt, P. Kliche, S. Klingebeil, A. M. Klodmann, J. Kniemeyer, O. Knobbe-Thomsen, C. B. Koch, A.-K.

40 50, 52 34 42 44, 45 45 45

P055 P220, P179 P139 P073 P155, P156 P161 P161

32 26, 27 24, 64 33 18, 31, 36, 40, 42, 53 34 44 15, 17 23, 24 44, 50 32 45 52, 54, 61 46 37 22, 29, 40 50 64 29 15 49 33 61 33 33 31 33, 39 49 30 52 43 29 31, 41 48 34

P124 PL29 YIA6 P136 P058, P205, P073, P226, P039 P192 P149 PL03 P152, P178 P125 P160 P239, YIA4, P222 P169 P213 P015, P062 P172 YIA6 P017 NC2 P120 P136 YIA4 P136 P131 P041 P051, P130 P118 P033 P221 P102 P023 P068, P038 P113 P192

90

Kocher, T. Kockmann, T. Koehler, C. Kögler, G. Kohlbacher, O. Kohrs, F. Kolarich, D. Kollipara, L. Kondo, T. Kopitz, J. Korf, U. Kostin, S. Koutroumpas, K. Kramer, G. Kraner, M. Krasnov, G. Krause, E. Krieg, N. Kriitanai, C. Krijgsveld, J. Kristiansen, G. Krizova, L. Krüger, C. Krüger, T. Kruse, F. Kudryavtseva, A. Kuhlmann, K. Kuhlmann, L. Kuhring, M. Kulkarni, V. Kuropka, B. Kustatscher, G. Kuster, B. Kux van Geijtenbeek, S. L Lahmann, J. Lalk, M. Lampen, A. Landthaler, M. Lane, L. Lange, P. F. Langridge, J. I. Larina, I.

Author List

31, 40 49 53 34 15, 33 30 43 45, 50 25 40 46 55 45 34 42 29 44, 52 34 41 18 40 41 55 31, 34, 41 32 29 33 61 29 58 52 18 25, 39, 43, 45 54

P044, P062 P120 P231 P144 P133 P025 P098 P158, P177 PL26 P061 P168 P246 P160 P141 P074 P017 P155, P221, P223 P142 P067 O05 P053 P072 P246 P068, P142, P038 P124 P017 P129 YIA4 P018 YIA1 P221 O04 P103, P159, P009 P242

34 32 44, 45 49 30 31 29, 31, 34, 36 46

P139 P049 P155, P156 P114 P032 P041 P015, P206, P141, P035 P169

91

Author List


Larsen, M. R. Lasonder, E. Laumen, H. Lavery, D. Lawless, C. Leber, Y. Lechner, J. Lee, H. Lee, K. A. Lee, V. Lehmann, R. Lehr, S. Lemeer, S. Lenobel, R. Lepper, M. Lescuyer, P. Lewis, S. Lichtenstein, D. Lichtenthaler, S. Lieberenz, M. Lievens, S. Li, L.-H. Lilley, K. Limonciel, A. Lindsey, M. Lisacek, F. Lisitsa, A. Liu, J. Liu, W. Liu, Y. Li, X. Li, X. Li, Y. Lobo, V. Lochmanová, G. Loipetzberger, A. Longen, S. Longone, P. Lopez, M. Lopez Villar, E. Lopiano, L. Lorenz, C. Loroch, S.

15, 17, 26 52 38 51 36 49 33 38 49 34 55 35, 44 39, 43, 45 38, 63 44 48 29 44, 45 33 45 39 45 18, 21 50 12 48 14, 29 36, 46 46 36 36 36 53 24, 58 42 33 43 51 50 16 36 41 23, 45

ED3, PL06 P216 P002 P187 P204 P116 P131 P002 P117 P141 P248 P149, P193 P103, P159, P009 P006, YIA5 P147 P112 P016 P155, P156 P135 P159 P012 P159 PL19 P177 HUPO05 P112 P017 P211, P163 P163 P211 P211 P211 P233 YIA1 P095 P133 P107 P189 P175 NC6 P204 P071 P162, O17

92

Losio, N. Lößner, C. Lottspeich, F. Lotz, J. Lotz, J. Lubeck, M. Lucacchini, A. Luch, A. Ludwig, C. Lu, J. Lundgren, C. Luptovciak, I. Lu, Q. Ly, A. M Macek, B. Machleidt, T. Macht, M. Magnusson, R. Majek, P. Malih, I. Maly, M. Manghina, V. Mann, M. Manns, A. Marchetti-Deschmann, M. Marcus, K. Marg, A. Mariappan, V. Mariman, E. Marogna, G. Martens, L. Martinez Jaime, S. Martin, F. Martin, F. C. Marx, H. Mastrobuoni, G. Mathieson, T. Matros, A. Mattern, D. Mayer, R. McMahon, K. Mecenas, D.

Author List

42 36 14, 21 39 39 40, 53 45 50 14 33 34 48 45 52

P079 P208

20 50 33, 40 45 44 51 44 47 18, 22, 25, 53 52 34, 35 44 50 51 53 32 26, 27, 30, 59 55 35 36 45 44 45 42 31 50 36 39

PL12 P172 P127, P056, P057 P157 P148, P150 P186 P148 P085 P226 P223 P192, P194 P153 P178 P182 P229 P046 YIA3, P025, P031 P251 P199 P210 P159 P152 P159 P073, PL20 P038 P180 P204 P013

P050 P050 P058, P226 P158 P174 ED2 P128 P139 P087 P160 P217

Author List


Mechtler, K. Medard, G. Meier, F. Meierhofer, D. Meixner, A. Mendes, M. Mendez, J. Menzler, J. Mercuri, N. B. Merkel, D. Merl-Pham, J. Messner, S. Meyer, H. E. Meyer, K. Miccolo, C. Michalak, M. Michalik, S. Michalski, A. Mik, V. Miladinovic, S. M. Milani, E. Milek, M. Millar, Harvey Minucci, S. Mischerikow, N. Mishto, M. Mittler, G. Mitulović, G. Mock, H.-P. Moez, P. Moghaddas Gholami, A. Mohamed, S. Moheb, M. Mohr, S. Mollah, S. Mollenhauer, M. Morgenroth, K. Moritz, C. Morrice, N. Morrison, E. Mostertz, J. Mouton-Barbosa, E. Muckova, P.

13, 29, 38, 41, 54 39 53 35 38 54 50 52 51 40 50, 51 29, 36 15, 25, 32, 33, 34, 40 31 49 40 32 40, 53 38 36 22 49 21, 23 49 38 35 48 42 23, 42 47 45 33 47 52 42 45 38 27, 32 16, 33 52 38, 43 30 34

P004, P234, P022, P064 P009 P226 P201 P003, P004 P236 P172 P217 P189 P057 P173, P183 P212, P024 P123, P129, P053, P144 P039 P121 P061 P049 P058, P226 P006 P212 O13 P114 PL17 P121 P004 P200 P109 P095 P073, PL20 P083 P159 P130 P083 P190 P094 P159 P007 P048 NC7 P221 P099, P007, P101, P102 P032 P142, P143

94

Mueller, M. Muenz, C. Mühlhaus, T. Müller, Andre Müller, G. Müller, H.-W. Müller, M. Münch, S. Munga, M. D. Munoz-San Juan, I. Murphy, N. Murr, A. Muth, T. N Nadler, W. Nau, K. Nebrich, G. Nemec, A. Nemitz, S. Nguyen, M. T. Nicolai, S. Niedzwiecka, A. Niehues, A. Niemann, B. Nitsche, A. Noberini, R. Nölker, R. Norgauer, J. Nury, C. Nyalwidhe, J. Nyman, E. O Obermann, J. Oellerich, M. Oellerich, T. Ohmayer, U. Olausson, P. Opialla, T. Opitz, S. Oppermann, M. Opravil, S. Opsomer, A. Oravec, M.

Author List

48 54 32 26 42 34 55 52 30 51 50 40 30

P112 P241 P048 O24 P093 P144 P248 P190 P027 P187 P172 P062 P025

24, 52, 61 42 39 41 34 45 50 44 53 45 38 49 43 34 35 31 45

YIA4, P222 P092 P051 P072 P142 P160 P174 P155 P227 P156 P005 P121 P099, P101, P102 P142 P199 P035 P157

51 52 52 51 32 50 34 30, 35, 50, 52 41 30 44

P184 P190 P190 P183 P126 P178 P142 P026, P219, P196, P175 P064 P032 P148

95

Author List


Orban-Nemeth, Z. Orfanos, Z. Orfao, A. Ori, A. Osiewacz, H. D. Osorio-Trujillo, J. C. Oswald, S. Othman, I. Otto, A. Otto, P. Ovaa, H. Ovecka, M. Overall, C. M. Overbeck, N. Ozel-Demiralp, F. D. P Paech, A. Pagel, K. Pagnozzi, D. Paki, K. Palmer, M. Palomba, A. Pani-Jankovi, T. Panse, C. Paron, I. Parte, P. Pecankova, K. Pechan, T. Peitsch, M. C. Pekar, S. Pelaseyed, T. Pengelley, S. Perocchi, F. Perske, C. Persson, H. Pesek, J. Pes, M. Petras, D. Petrovska, B. Pfeilschifter, J. Pförtner, H. Philipp, O. Phillips, B.

54 49 55 33 53 34, 44 35 51 47 50 39 48 25, 31 48 31

P234 P116 P249 P134 P225 P154, P138 P203 P186 P081 P172 P008 P087 P041 P113 P043

35 22 32, 47, 51 50 29 47 42 53 53 58 44 48 35 31 43 43 51 52 39 34 32 31 63 43 32 53 35

P193 P046, P047, P181, P084, P085 P176 P015 P084, P085, P086 P095 P230 P226 YIA1 P148, P150 P087 P199 P037 P097 P098 P183 P190 P011 P047 P142 P043 YIA5 P107 P049 P225 P199

96

Author List

Piano, F. Pich, A. Pienimaeki Roemer, A. Pietsch, J. Pike, I. Ping, Peipei Piras, C. Pirisi, A. Pisanu, S.

39 36, 45 33 55 36 13, 17 35, 42, 51 32 32, 47, 51

Pitt, Andrew Pizzi, E. Planatscher, H. Plucienniczak, A. Plucienniczak, G. Plückthun, A. Poetsch, A. Polten, F. Pongthanom, N. Pontén, F. Ponzi, M. Pope, J. Poschmann, G. Potesil, D. Pötz, O. Prescott, A. Pruneri, G. R Rabe, A. Rabus, R. Rackwitz, T. Radiziwill, G. Radler, P. Raether, O. Raffai, T. Rahm, M. Ralser, M. Ramadoss, D. Ramallo Guevara, C. Rampler, E. Ramsauer, C. Rant, U. Rao, P.

13 54 52 31 31 44 53 36 41 45 54 29 42 42 12, 17, 45, 50, 52 55 49 31 31 50 49 33 40, 53 49 43 22 35, 53 15, 53 54 54 15 58

P013 P207, P161 P129 P245 P208 HUPO09, PL05 P079, P195, P189 P047 P045, P046, P047, P181, P085, P086 P240 P220 P042 P042 P146 P225 P207 P067 P159 P240 P015 P092 P078 P162, P220, HUPO 6, P179 P250 P121 P044 P036 P180 P116 P136 P058, P226 P118 P105 P197, P224 P225 P234 P243 NC3 YIA1

97

Author List


Rapp, E. Rappsilber, J.

30 30, 40, 54

Raso, C. Regine, H. Regitz-Zagrosek, V. Regl, C. Reichl, U. Reifenberger, G. Reimann, L. Reimer, U. Reinders, J. Reindl, J. Reinhold, P. Reiter, L. Renard, B. Y. Renard, P. Renfree, M. Ren, J. M. Ren, X. Rexroth, S. Rezaei-Tavirani, M. Rhim, J. Rhode, H. Richter, E. Richter, F. Riedel, K. Riedelova-Reicheltova, Z. Rieder, V. Ried, T. Riemer, A. B. Rigbolt, K. Rinner, O. Riwaldt, S. Robers, M. Robinson, M. Roblick, U. J. Rocca, S. Roepman, R. Roepstorff, P. Roesli, C. Rogers, L. D. Rögner, M.

32 50 12 42 30 32 49 44, 45 19 34 34 22, 29, 36, 37, 43 26, 29 38 36 49 46 53 49 31 34 26, 38, 43 43, 48, 55 20 44 32 34 33 44 22, 29, 36 55 50 48 34, 35, 44 32 45 14 43 31 53

P025 P054, P028, P236, P237, P029, P238 P125 P174 HUPO02 P096 P025 P124 P116 P152, P159 O09 P142 P143 P106, P212, P214, P024 O19, P018 P001 P210 P117 P163 P225 P170 P035 P142, P143 P099, P007, P102, O23 P247, P107, P109 PL13 P148 P124 P139 P132 P151 P212, P024 P245 P172 P088 P139, P193, P149 P045, P046 P160 P103 P041 P225

98

Roncada, P. Ronci, M. Röring, M. Rosenblatt, M. Rösli, C. Rozycki, C. Ruprecht, B. Rurik, M. Russell, D. Russell, R. B. S Saba, J. Sabino, F. Sadewasser, A. Saha, B. Saile, R. Saliba, P. Säll, A. Samaj, J. Samyn, N. Sanchez Rodriguez, F. E. Santacoloma-Osorio, M. Sass, S. Savitski, M. M. Schaab, C. Schaefer, A. Schaefer, J. Scherbaum, V. Scherl, A. Schillo, K. Schira, J. Schlage, P. Schlapbach, R. Schlegl, J. Schloegelhofer, P. Schlosser, A. Schlüter, R. Schmidt, C. Schmidt, F. Schmidt, G. Schmit, P.-O. Schmitz, G. Schnatbaum, K. Schneider, H.

Author List

22, 35, 42, 51 45 44 16 33, 52, 54, 61 45 39, 43 33 55 45

P079, P195, P189 P158 P151 NC5 P239, P132, YIA4, P222 P156 P103, P009 P133 P250 P160

35, 52 49 50 41 51 15, 40 39 48 39 47 51 32 45 51 43 44 48 48 34 34 49 53 45 54 31, 48 32 51 32 46 31, 36 20, 26, 33 44, 45 43

P196, P219 P120 P176 P070 P186 P060, NC1 P011 P087 P012 P082 P188 P124 P159 P187 P105 P146 P113 P112 P140 P144 P120 P230 P159 P234 P040, P110 P049 P187 P049 P166, P167 P039, P205 P129, O21 P152, P159 P097

Author List


Schneider, M. Schneider, R. Schneider, T. Schnölzer, M. Scholten, A. Schöndorf, D. C. Schott, A.-S. Schriemer, D. Schrötter, A. Schrottmaier, W. Schuhmacher, C. Schulenborg, T. Schümann, M. Schurr, B. C. Schutzbier, M. Schütz, E. Schwamb, B. Schwämmle, V. Schwarzwälder, A. Schweiger-Hufnagel, U. Schwenk, Jochen Schwinn, M. Scigelova, M. Sebela, M. Sedlmeier, E.-M. Sedo, O. Seelk, S. Segerer, J. Seidel, P. Seifert, J. Selbach, M. Selve, S. Senkbeil, H. Senkler, M. Senninger, N. Serve, H. Seymour, S. Sferra, G. Sharma, V. Shaw, G. Sickmann, A. Sieg, H. Siegmund, W. Sievers, H. H.

54 50 35 40, 50 39 38 41 27 32 46 44 32 44 41 41 52 40 18 55 36, 43 12, 13 50 52 63 44 30, 31, 41 53 55 50 41 39, 41, 49, 50, 53 44 31 29 50, 52 52 40 54 52 36 13, 14, 16, 17, 45, 50, 54, 55 44, 45 35 33

P236, P237 P179 P199 P061, P180 P008 P003 P065 PL33 P123 P167 P146 P048 P155 P065 P064 P190 P059 O03 P245 P205, P098 HUPO01 P172 P218 YIA5 P147 P033, P072, P037 P228 P245 P180 P069 P114, P013, P176, P063, P228 P155 P044 P023 P217, P173 P190 P057 P240 P216 P210 P245, P158, P235, P162, P177 P155, P156 P203 P130

100

Silva, J. C. Simerský, R. Simon, M. Simon, S. Singh, S. Sinz, A. Sitek, B. Sjöström, D. Skalický, V. Skeffington, A. Slevogt, H. Slotta-Huspenina, J. Smekalova, V. Smola, H. Soggiu, A. Soher, E. Solari, F. A. Solis, N. Soll, J. Sommersdorf, C. Sonnewald, U. Sonntag, J. Spalloni, A. Spuler, S. Srbova, E. Srnad, M. Stefanski, A. Steger, M. Stejskal, K. Stengel, F. Stentzel, S. Stephan, C. Stokes, M. P. Stopka, P. Stopkova, R. Strålfors, P. Stranzl, T. Strasser, L. Strauss, J. Strecker, V. Streng, A. Strohkamp, S. Strohschein, K.

Author List

49 38 32 54 17, 23 21 40 32 38 48 55 45 48 49 20, 35, 42 35 19 31 47 50 42 46 51 50 42 38 48 40, 48 42 27 32 35 49 30 30 45 54 54 49 40 53 34, 44 39

P117 P006 P049 P242 PL22 PL14 P053 P126 P006 P090 P248 P159 P087 P120 P079, O12, P195 P194 O08 P041 P080 P179 P074 P168 P189 P178 P078 P006 P113 P110, P059 P078 O27 P049 P194 P117 P033 P033 P157 P234 P243 P115 P059 P229 P140, P149 P051

101

Author List


Stühler, K. Stunnenberg, H. G.

Suckau, D. Suemchen, P. Sugihara, Y. Sukop-Köppel, U. Surmann, K. Süssmuth, R. Suttnar, J. Swart, C. Szibor, M. Szymczak, S. T Taborda-Ocampo, G. Tacheny, A. Tahir, S. Takac, T. Talamás-Rohana, P. Tanca, A. Tascher, G. Tavernier, J. Tee, T. Y. Tenzer, S. Teufl, J. Texier, Y. Textor, B. Theis, F. The Syscilia Consortium EU Thiele, H. Thierse, H.-J. Thiyagarajan, V. Thomas, B. Thorns, C. Thriene, K. Thünemann, A. Tigges, J. Tilocca, B. Timmerman, E. Tinnefeld, V. Titeca, K. Titz, B. Toledo-Leyva, A. Tölle, R.

27, 32, 34, 42, 48 52 44 52 20 43 32 31 44 48 55 34, 35

P146, P092, P124, P144, P113 P146 P219 O11 P100 P049 P043 P148 P090 P246 P139, P193

51 38 54 48 34, 44 47, 51 35 39 51 14, 22, 32 33 45 49 32 45 33, 39 50 35, 53 33 34, 35 46 44 32 41 39 54 39 35 34 36

P188 P001 P238 P087 P154,P138 P181, P084, P085, P086 P202 P012 P186 P048, ED1 P136 P160 P117 P124 P160 P050, P051, P130 P174 P197, P224 P131 P140, P193 P165 P155 P124 P069 P012 P235 P012 P199 P138 P209

102

Author List

Toro, N. D. Towers, M. Toyama, B. Trachsel, C. Trede, D. Treue, D. Trost, M. Tschöp, M. Tsybin, Y. Turkovic, D. Tursun, B. U Ueffing, M.

29 39 33 53 39 33 55 42 17 36 53

P016 P052 P134 P230 P050, P051 P131 P250 P093 PL04 P206 P228

25, 28, 32, 38, 43, 45, 52

Uhlen, M. Ulbrich, D. Upadhyay, A. Urbani, A. Urinovska, J. Urlaub, H. Uthe, H. Uzzau, S.

45 43 35 13, 16, 19, 35, 42, 45, 51 63 21, 52, 64 31 32, 47, 51

P125, P003, P004, P160, P105, P217 P159 P101, P102 P197 P158, P079, P195, P189 YIA5 YIA6, P190, PL16 P040 P045, P046, P047, P181, P084, P085, P086

V Vadivelu, J. Vadovic, P. Valiante, V. van Beersum, S. Van Calenbergh, S. van den Berg, B. H. J. Vandenbrouck, Y. Vandepoele, K. van der Ven, P. van Dieijen-Visser, M. Van Dorsselaer, A. van Eyk, Jennifer van Kasteren, S. Van Quickelberghe, E. van Reeuwijk, J. Vanscheeuwijck, P. Vanselow, J. T. van Soolingen, D. van Veelen, P.

51 48 41 45 39 52 30 29 49 53 30, 35 13 39 39 45 35 31 59 59

P182 P087 P068 P160 P012 P220 P032, O06 P014 P116 P229 P032, P202 HUPO 10 P008 P012 P160 P199 P040 YIA2 YIA2

103

Author List


Vasiljevic, D. Vellasamy, K. M. Venne, S. Ventz, K. Verhee, A. Verheggen, K. Verhelst, S. Villegas-Pineda, J. C. Viner, R. Vinkler, D. Vissers, J. P. Vitek, O. Vizcaino, J. A. Vogel, R. F. Völker, U. von Eggeling, F. von Mering, C. von Törne, C. W Wachter, A. Waidelich, D. Waldemarson, S. Waldera-Lupa, D. M. Wallner, S. Walter, U. Walther, D. Wang, D. Wang, R. Wang, X. Ward, M. Warnken, U. Warscheid, B. Wawrzyniak, P. Weber, G. Wehland-von Trebra, M. Weibchen, G. Weilnböck, L. Weiss, F. Wendler, S. Wenschuh, H. Wicht, J. Wiese, H. Wildgruber, R.

53 51 47 43 39 29 21, 39, 54 34 52 30 29, 34, 36, 39 36 29 41 20, 31, 32, 40, 46 39 25 15, 38, 44, 52

P228 P182 P080 P099 P012 P019 P235, P010 P138 P218 P033 P015, P204, P052, P206, P141 P212 P016 P065 P164, P044, P062, P049 P050 PL25 P002, P147, P217

46 40 39 32, 34 12 45 23, 48 45 29 33 36 50 49 31 43 55 36 33, 42 52 34 44, 45 52 49 43

P168 P056 P011 P124, P144 HUPO08 P162 O18, P089 P159 P016 P127 P208 P180 P116 P042 P100 P245 P204 P133, P096 P220 P142, P143 P152, P159 P190 P116 P100

104

Author List

Wilhelm, M. Williams, E. Williamson, N. Wimmer, B. Winkler, T. Winter, M. Winter, S. Wissinger, Y. Wittig, I.

45 53 36 33 39 50 34 38 35, 40, 43, 48, 55

Wodzig, W. Wöhlbrand, L. Wolff, T. Wollert, K. Wood, K. Woodroofe, C. Wühl, M. Wu, Y. X Xia, B. Xiong, L. Xuan, Y. Y Yang, X. Yan, J. Yates, J. Yildirim, A. Yildiz, M. Z. YingYing, C. Z Zahedi, R. P. Zaleski, P. Zanotelli, V. Zauber, H. Zdráhal, Z. Zecha, J. Zeiser, J. Zellner, M. Zenobi, R. Zetterberg, H. Zezzi-Arruda, M.-A. Zhang, H. Zhang, Y.

53 31 50 36 50 50 33 53

P159 P232 P210 P136 P051 P137 P180 P004 P201, P246, P247, P107, P109, P059 P229 P036 P176 P207 P172 P172 P132 P232

46 42 22

P163 P094

46 33 13, 16 50 31 41

P163 P130 PL01 P173 P043 P066

25, 45, 47, 48 31 36 41 30, 31, 41, 42 39 45 26, 46 44 36 51 33 54

P111, P158, P162, P080, P235 P042 P212 P063 P033, P072, P037, P078, P095 P009 P161 O22, P167 P146 P208 P188 P127 P239

105

Author List


Zhou, C. Zhuang, Z. Zibetti, M. Ziegler, A.-G. Ziegler, E. Zimmermann, K. Zimmermann, P. Zörnig, M.

50 36 36 44 45 50 29 40

P180 P211 P204 P147 P159 P172 P024 P059


Proteomic Forum 2017 Spring 2017 Berlin

Hosting Society German Society for Proteome Research c/o ISAS Bunsen-Kirchhoff-Straße 11 44139 Dortmund (DE) www.dgpf.org

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