proteins by proton nuclearmagnetic resonance (NMR) spectroscopy has been ... Another problem unrelated to the analytical ...... and labor-intensive methods.
CLIN.CHEM. 37/3, 377-386(1991)
Quantification of Plasma Lipoproteins by Proton Nuclear Magnetic Resonance Spectroscopy J. 0. OtvOs,1 E. J. Jeyarajah,1and 0. W. Bennett A new analytical procedure for quantifying plasma lipoproteins by proton nuclearmagnetic resonance (NMR) spectroscopy has been developed thatpotentially offers significant advantages over existing clinical methods used for assessing riskofcoronary heart disease. Analysis of a
singlespectrum of a nonfasting plasma sample,acquired simplyand rapidlyat moderate magneticfieldstrength (250 MHz), yieldsa completeprofile of lipoprotein concentrations: chylomicrons and very-low-, low-, and highdensitylipoproteins. The method is based on curve-fitting (spectral deconvolution) of the plasma methyllipid resonance envelope, the amplitude and shape of which depend directly on the amplitudes of the superimposed methyl resonances of the lipoprotein components. A linear least-squares curve-fitting algorithm was developed to efficiently extract the signalamplitudes(concentrations) of the lipoproteins from the plasma spectrum. These signal amplitudes correlate well with lipoprotein concentrations determined by triglyceride and cholesterol measurements. AddItIonal
Keyphrases:
cholesterol
triglyceride
cuive-fit-
ting algorithm
The preventionofcoronaryheart disease (CHD) is a major publichealth objective that relies, in part,on accurate identffication of individuals at increased risk.2 This is currently being accomplishedby screeningthe concentrationsof cholesteroland lipoproteinlipids in blood, according to guidelines recommended by the Expert Panel of the National CholesterolEducation Program (NCEP) (1). Initialclassification intothree categories of CHD risk is based on the concentration of total cholesterol: “desirable” (
