Quantitative Immunoenzymatic Assay of Human ... - Clinical Chemistry

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Sep 5, 1989 - assay; HAT, hypoxanthine-aminopterin--thymidine;. ONPG, o-nitrophenyl--. D-galactopyranoside; PBS, phosphate-buffered saline; BSA, bovine.

CLIN. CHEM. 36/1, 47-52 (1990)

Quantitative Immunoenzymatic Assay of Human Lutropin,with Use of a Bi-Specific Monoclonal Antibody GlovannaBugarl,1ClaudloPoIesl,1 Alberto Beretta,2 Salvatore GhIeImI,1 and Alberto AIbertInI1 In this immunoenzymatic assay for human lutropin (hLH) we

used bi-specific antibodies (BiAbs) obtained from the fusion of two hybndomas producing antibodies to 13-D-galactosidase and hLh. The BiAb complexed with the enzyme (3-D-galactosidase was used as tracer in a double-determinant assay. We compared the assay involving the BlAb (Bi-EIA) with an immunoenzymatic

assay (EIA) in which the same capture

antibody was used but the tracer was an enzyme-conjugated hLH-specific monoclonal antibody produced by the same parental cell line used to produce the BiAb. The coefficient of correlation (r) between the two assays was 0.979 but the Bi-EIA was more sensitive (detection limits: 0.8 mt. units/L for the Bi-EIA, 2.0 mt. units/L for the EIA) and more specific (

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