RAKA MUSTAPHI MITRA

RAKA MUSTAPHI MITRA Department of Biology Carleton College One North College Street Northfield, MN 55057 U.S.A.

Phone: (507) 222-4120 Fax: (507) 222-5757 Email: [email protected] http://apps.carleton.edu/people/rmitra/

PRESENT POSITION 2016-Present

Associate Professor, Biology Department, Carleton College

2008-2016

Assistant Professor, Biology Department, Carleton College

EDUCATION 1996-2003

Stanford University, Stanford, CA, Ph.D., Biological Sciences

1992-1996

Massachusetts Institute of Technology, Cambridge, MA, S.B., Biology.

RESEARCH EXPERIENCE 2004-2008

Postdoctoral Fellow, University of Minnesota (Research Advisor: Dr. Jane Glazebrook) Studies of the plant defense response to pathogens of leaves and roots.

2003-2004

Postdoctoral Researcher, Stanford University (Research Advisor: Dr. Sharon Long) Studies of plant symbiotic gene expression and identification of the Medicago truncatula DMI3 gene using transcript-based gene cloning.

1996-2003

Graduate Student, Stanford University (Research Advisor: Dr. Sharon Long) Studies of early plant gene expression and calcium signaling in the Rhizobiumlegume symbiosis.

1995-1996

Research Assistant, Massachusetts Institute of Technology (Research Advisor: Dr. Harvey Lodish) Investigation of the role of Rab4 and Rab5 in recycling of the Glut4 glucose transporter in adipocytes.

1994-1995

Research Assistant, Massachusetts Institute of Technology (Research Advisor: Dr. Jonathan King) Studies of the effects of mutant scaffolding proteins on the assembly of bacteriophage P22.

1993-1994

Research Assistant, University of Minnesota (Research Advisor: Dr. Glenn Furnier) RAPD analyses of genetic variation of the Butternut canker fungus Sirococcus clavigignenti-juglandacearum.

1991-1992

Research Assistant, University of Minnesota (Research Advisor: Dr. Glenn Furnier) Isozyme analyses of Eurasian watermilfoil (Myriophyllum spicatum L.)

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TEACHING EXPERIENCE Winter 2009 - present

Directed Research in Biology (BIOL 394), Carleton College Responsibilities include designing and supervising undergraduate research projects to examine the role of pathogenic effectors of the bacterial pathogen of plants, Ralstonia solanacearum. Total number of students supervised to date: 22.

Spring 2009 - present

Cell Biology and Lab (BIOL 280, BIOL 281), Carleton College A mid-level course that examines of the structures and processes that underlie life in prokaryotic and eukaryotic cells. Includes two weekly lab components in which students perform original research investigating the cell biology of plant microbe interactions.

Winter 2009 - present

Energy Flow in Biological Systems and Lab (BIOL 126, BIOL 126L), Carleton College An introductory course designed for both majors and non-majors. Explores the flow of energy in biological systems from molecules to ecosystems. Includes a weekly lab component. Co-taught with J. Tymoczko (2009, 2011, 2013, 2014), D. Hernández (2010), D. HougenEitzman (2015).

Fall 2008 – present

Microbial Pathology (BIOL 385), Carleton College An upper level course, for juniors and seniors. Goals include teaching students how to critically read and understand primary research papers in the field of microbial pathology. Students learn about the scientific publication process, pathogenesis proteins, biological processes and scientific techniques used to study microbial pathogenesis.

Winter 2009 –present

Biology Integrative Exercise, Carleton College A senior capstone project for which students investigate a particular biological question.

Fall 2006

Genetically Modified Plants: the Science and the Controversy (BIOL 4950), University of Minnesota An upper level course for undergraduate and graduate students to examine the intersection of science and policy, focusing on the science behind genetically modified plants. Co-taught with R. Van Poecke and D. Lohar.

Fall 2005, 2006

Genetics (BIOL 4003), Guest Lecturer, University of Minnesota Presented lectures on various topics including Genomics and DNA replication.

2005-Present

Directed Research (BIOL 4994), Supervisor, University of Minnesota Responsibilities included designing undergraduate research projects to identify plant genes important for pathogen defense, and advising several undergraduate students.

Winter 1999

Cell Biology: Molecular Organization (BIO 129B), Teaching Assistant, Stanford University Responsibilities included leading a weekly one-hour discussion section, conducting problem-solving demonstrations, grading and composing problems for exams, and tutoring individual students.

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Spring 1999

Cell Biology: Cellular Dynamics (BIO 129A), Teaching Assistant, Stanford University Responsibilities included leading a two-hour paper discussion section every other week, meeting with students individually to discuss presentations and grading exams.

Winter 1998

Biochemistry, Molecular Biology and Cell Biology (BIO 41), Teaching Assistant, Stanford University Course: Biochemistry, Molecular Biology and Cell Biology Responsibilities included leading two one-hour discussion sections per week, preparing handouts, grading exams and tutoring individual students.

PUBLICATIONS (*denotes undergraduate co-authors) Peterson EK*, Mitra RM. Enhancing Interactions between Research Lab Students & Their Communities. The American Biology Teacher. 2016 Aug 1;78(6):509-11. Jacobs JM, Milling A, Mitra RM, Hogan CS, Florent Ailloud F, Prior P, Allen C. Ralstonia solanacearum requires PopS, an ancient AvrE-family effector, for virulence and to overcome salicylic acid-mediated defenses during tomato pathogenesis. MBio. 2013 Nov 26;4(6). Wang L, Mitra RM, Hasselmann KD, Sato M, Lenarz-Wyatt L, Cohen JD, Katagiri F, Glazebrook J. The genetic network controlling the Arabidopsis transcriptional response to Pseudomonas syringae pv. maculicola: roles of major regulators and the phytotoxin coronatine. Mol Plant Microbe Interact. 2008 Nov;21(11):1408-20. Middleton P, Jakab J, Penmetsa V, Starker C, Doll J*, Kalo P, Prabhu R, Marsh J, Mitra RM, Kerestz A, Dudas B, VandenBosch K, Long SR, Cook D, Kiss G, Oldroyd GED. An ERF transcription factor in Medicago truncatula that is essential for Nod factor signal transduction. Plant Cell. 2007 Apr; 19(4):122134. Marsh JF, Rakocevic A, Mitra RM, Brocard L, Sun J, Eschstruth A, Long SR, Schultze M, Ratet P, Oldroyd GED. Medicago truncatula NIN is essential for nodule organogenesis induced by autoactive calcium/calmodulin dependent protein kinase. Plant Physiol. 2007 May; 144(1) 324-335. Sato M, Mitra RM, Coller J, Wang D, Spivey NW, Dewdney J, Denoux C, Glazebrook J., Katagiri F. A high performance, small-scale microarray for expression profiling of many samples in Arabidopsispathogen studies. Plant J. 2007 Feb; 49 (3) 565-567. Starker CG, Parra-Colmenares AL, Smith L, Mitra RM, Long SR. Nitrogen fixation mutants of Medicago truncatula fail to support plant and bacterial symbiotic gene expression. Plant Physiol. 2006 Feb; 140 (2): 671-680. Kaló P, Gleason C, Edwards A, Marsh J, Mitra RM, Hirsch S, Jakab J, Sims S, Long SR, Rogers J, Kiss GB, Downie JA, Oldroyd GE. Nodulation signaling in legumes requires NSP2, a member of the GRAS family of transcriptional regulators. Science. 2005 Jun 17;308(5729):1786-1789. Mitra RM, Shaw SL, Long SR. Six nonnodulating plant mutants defective for Nod factor-induced transcriptional changes associated with the legume-rhizobia symbiosis. Proc. Natl. Acad. Sci. U.S.A. 2004 Jul 6;101(27):10217-10222. 2+

Mitra RM, Gleason CA, Edwards A, Hadfield J, Downie JA, Oldroyd GE, Long SR. A Ca /calmodulindependent protein kinase required for symbiotic nodule development: Gene identification by transcriptbased cloning. Proc. Natl. Acad. Sci. U.S.A. 2004 Mar 30;101(13):4701-4705.

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Mitra RM, Long SR. Plant and bacterial symbiotic mutants define three transcriptionally distinct stages in the development of the Medicago truncatula/Sinorhizobium meliloti symbiosis. Plant Physiol. 2004 Feb; 134 (2): 595-604. Engstrom EM, Ehrhardt DW, Mitra RM, Long SR. Pharmacological analysis of Nod factor-induced calcium spiking in Medicago truncatula. Plant Physiol. 2002 Apr; 128(4):1390-1401. Oldroyd GE, Mitra RM, Wais RJ, Long SR. Evidence for structurally specific negative feedback in the Nod factor signal transduction pathway. Plant J. 2001 Oct; 28(2):191-199. Furnier GR, Stoltz AM, Mustaphi RM, Ostry ME. Genetic evidence that butternut canker was recently introduced into North America. Can. J. Bot. 1999 June; 77(6):783-785. Furnier GR, Mustaphi MM. Isozyme variation in Minnesota populations of Eurasian watermilfoil. Aquatic Bot. 1992 Oct; 43(3):305-309. GRANTS AWARDED National Science Foundation Biology Division of Integrative Organismal Systems “RUI: The role of Ralstonia solanacearum effector protein Rsp1281 in bacterial wilt disease.” (NSF IOS #1257479, 2013present, $450,000) Curricular Development Grant, Carleton College, “Integrating Microscopy Facilities into the Carleton College Cell Biology Labs.” (2012) Large Faculty Development Grant, Carleton College, “The study of plant resistance to the bacterial wilt pathogen Ralstonia solanacearum.” (2011-2012) Curricular Development Grant, Carleton College, “Familiarizing students with the scientific publication review process.” (2009) Howard Hughes Medical Institute Curricular Project Grant, Carleton College, “Analysis of publicly available genomic datasets: Transcriptomics in the Carleton Cell Biology Labs” (2009) AWARDS AND FELLOWSHIPS American Society of Cell Biology Faculty Travel Award (2013) American Society of Cell Biology Childcare Award (2013) American Society of Plant Biology Travel Award (2012) Class of ’49 Fellowship, Carleton College, “The study of plant resistance to the bacterial wilt pathogen Ralstonia solanacearum.” (2011-2012) Mellon Fellowship, Carleton College “The study of plant resistance to the bacterial wilt pathogen Ralstonia solanacearum.” (2011-2012) National Institutes of Health Postdoctoral Fellowship (2006-2008) Frances Lou Kallman Award for Graduate Study in Biological Sciences, Stanford University (2004) Howard Hughes Medical Institute Pre-doctoral Fellowship (1996-2001)

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MEETINGS ATTENDED July 2016 June 2016 July 2015 August 2014 December 2013 July 2012 August 2011 October 2009 July 2009 November 2008 November 2008 July 2007 July 2006 June 2005 June 2002 July 2001 July 2001 August 1999

th

17 International Congress on Molecular Plant-Microbe Interactions th 6 International Bacterial Wilt Symposium Plant Biology 2015 2014 American Phytopathological Society, Canadian Phytopathological Society Joint Meeting American Society of Cell Biology 2013 Annual Meeting Plant Biology 2012 Plant Biology 2011 Presenting Data and Information, Course by Edward Tufte th 14 International Congress on Molecular Plant-Microbe Interactions Council on Undergraduate Research Institute: Beginning a Research Program in the Natural Sciences at Predominantly Undergraduate Institutions. NSF iPlant collaborative Grand Challenge Workshop on Molecular Modeling. th 13 International Congress on Molecular Plant-Microbe Interactions th 4 International Bacterial Wilt Symposium. th 16 International Conference on Arabidopsis Research. First International Conference of Legume Genomics and Genetics: Translation to Crop Improvement. th 10 International Congress on Molecular Plant-Microbe Interactions th The 4 Workshop on Medicago truncatula th 9 International Congress on Molecular Plant-Microbe Interactions

INVITED SEMINARS February 2016 August 2015 July 2015 July 2012 February 2012 October 2011 May 2011 April 2011 March 2011 August 2010 November 2009 March 2009 November 2008 February 2007 November 2006 February 2006 March 2005

Carleton College, Biology Department Seminar Series Carleton TEA talk Plant Biology 2015 Plant Biology 2012 Wartburg College, Biology Seminar University of Wisconsin, Madison, Plant Pathology Department Seminar Series University of Minnesota Plant Biology Retreat, Career Seminar Minnesota State University, Mankato Biological Sciences Department Seminar Series University of Vermont, Plant Biology Department Seminar Series Carleton TEA talk St. Olaf College, Biology Department Seminar Series Macalester College, Biology Department Seminar Series Carleton College, Women in Action Panel University of Wisconsin, River Falls, WI, Biotechnology Seminar Series Minnesota State University, Mankato, MN Biology Department Seminar Series University of Saint Thomas, Saint Paul, MN Biology Department Seminar Series College of Saint John and Saint Benedict, Saint Cloud, MN. Biology Department Seminar Series.

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PRESENTATIONS INVOLVING STUDENTS (*denotes co-authors who are undergraduates or recent graduates) June 2016

The bacterial wilt pathogen, Ralstonia solanacearum: Effector proteins and plant root responses. Raka M. Mitra, Marie Schaedel*, Liza Peterson*, Anne E. Duncan*, Jonathan J Jacobs, William Truman, Jane Glazebrook, Fumiaki Katagiri, Caitilyn Allen. Talk presented by R.M. th Mitra at the 6 International Bacterial Wilt Meeting.

July 2015

The bacterial wilt disease of plants: studies of bacterial effector proteins and plant root responses to Ralstonia solanacearum Marie Schaedel*, Khuaten Maaneb de Macedo*, Calvin Phan*, Ka Thao*, William Truman, Jonathan J. Jacobs, Caitilyn Allen, Fumiaki Katagiri, Jane Glazebrook , Raka M. Mitra. Poster presented by M. Schaedel, K. Maaneb de Macedo, C. Phan, and K. Thao at the Plant Biology 2015 Meeting.

December 2014

The bacterial pathogen Ralstonia solanacearum attacks the plant by targeting different regions of the plant cell. Jacky Lu*, Emiri Matsuda*, Darcy Mishkind*, Anne Duncan*, Raka M. Mitra. Poster presented by J. Lu, E. Matsuda and D. Mishkind at the 2014 American Society of Cell Biology Annual Meeting.

August 2014

Ralstonia solanacearum provokes gene expression changes in Arabidopsis roots and targets different regions of the plant cell. Raka M. Mitra, Anne Duncan*, William Truman, Jacky Lu*, Rose Chen*, Darcy Mishkind*, Jane Glazebrook, Fumiaki Katagiri. Poster presented by A. Duncan, J. Lu, R. Chen and D. Mishkind at the 2014 American Phytopathological Society, Canadian Phytopathological Society Joint Meeting.

December 2013

The bacterial wilt pathogen, Ralstonia solanacearum: Effector proteins and plant root responses. Raka M. Mitra, William Truman, Nana Tanamoto*, Jane Glazebrook, Fumiaki Katagiri. Poster presented by R.M. Mitra at the 2013 American Society of Cell Biology 2013 Annual Meeting.

August 2011

The bacterial wilt pathogen, Ralstonia solanacearum: Effector proteins and plant root responses. Anne Duncan*, Nana Tanamoto*, Fumiaki Katagiri, Jane Glazebrook, Raka M. Mitra. Poster presented by A. Duncan, N. Tanamoto and R.M. Mitra at the Plant Biology 2011 Meeting.

November 2010

“A Glimpse of Bacterial Effector Proteins: Insight to an Agricultural Menace”, Michael Einstein*, Raka M. Mitra. Talk presented by M. Einstein at the Seven Rivers Undergraduate Symposium, Vitterbo University, Wisconsin. Received an award for an outstanding student presentation.

November 2009

Characterization of the Role of Effector Proteins of Ralstonia solanacearum in Bacterial Wilt Disease”, Katie A. Benson*, Musetta A. Steinbach*, Raka M. Mitra. Talk presented by K. Benson at the Seven Rivers Undergraduate Symposium, Vitterbo University, Wisconsin. Received an award for an outstanding student presentation.

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PROFESSIONAL SOCIETIES American Association for the Advancement of Science American Phytopathological Society American Society for Cell Biology American Society of Plant Biologists International Society for Molecular Plant-Microbe Interactions PROFESSIONAL SERVICE 2013 2010-present 2009-present 2005-2010

Table Leader for Career Discussion and Mentoring Roundtable on Teaching and Research in Primarily Undergraduate Institutions. ASCB 2013 Annual Meeting Manuscript reviewer for American Biology Teacher, Molecular PlantMicrobe Interactions and Plant Physiology, Member of several evaluation panels for Research Grants and Graduate Fellowships, National Science Foundation Educational Counselor, Massachusetts Institute of Technology

SERVICE AT CARLETON COLLEGE 2016-present Institutional Biosafety Committee 2016-present Career Center Committee 2014- 2016 Pre-health committee 2015 Assembled a HHMI Research Cohort at Carleton College 2014-2016 Budget Committee, Elected Non-tenured Member 2014 Assembled a HHMI Research Cohort at Carleton College 2012-2014 Junior Faculty Affairs Committee 2010-2011 Distinguished Women Visitor Fund Committee 2010-2011 Faculty Judiciary Committee

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