semipermeable membrane at the end of the probe. Small molecules and proteins diffuse across the size-selective membrane and are ... power. Surface tension is the tangential force that keeps a fluid together at the air/fluid interface. It is the ...
semipermeable membrane at the end of the probe. Small molecules and proteins diffuse across the size-selective membrane and are ... power. Surface tension is the tangential force that keeps a fluid together at the air/fluid interface. It is the ...
KEYWORDS: Rapid prototyping, self alignment, COP (cyclic olefin polymer) ... the film from bending or wrinkling and hence, to transfer the 2D CAD design as ...
Jan 11, 2007 - epoxy master for subsequent replication of the polymer chips by embossing or injection molding. During post-processing, the microfluidic.
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on a hard drive. Migrating cells were tracked ... Downington, PA) and data further analyzed using Matlab software (The ... membrane with dead-end channels in continuation of the ..... exposure to chemokine gradient or the recovery of direc-.
Sep 24, 2012 - Label-Free Microscale Thermophoresis Discriminates Sites and. Affinity of ProteinâLigand Binding**. Susanne A. I. Seidel, Christoph J.
May 11, 2015 - General Hospital, Winnipeg, MB, R2V 3M3, Canada, 6 Rizhao Hospital of .... recruiting staff employed at the Seven Oaks General Hospital in ...
Thermal injury can trigger a fulminant inflammatory cascade that can lead to sepsis and ... Our study on neutrophils from eight burn patients revealed impaired ...
Feb 8, 2012 - (LB) broth (Becton Dickinson and Company, Sparks, USA) cul- ..... Kaatz, G. W., Moudgal, V. V., Seo, S. M., and .... Expert Opin. Investig.
Jun 30, 2014 - Hector A. Cabrera-Fuentes. 1. , Florian Veit. 3. , Dongsheng Jiang. 4 ..... Lochnit G, Barreto G, et al. Neutrophil extracellular traps directly induce ...
Rapid and continuous magnetic separation in droplet microfluidic devices ... The microfluidic set-up is based on an inverted microscope (Motic, AE31) equipped ...
A Label-Free, Quantitative Assay of Amyloid Fibril Growth. Based on Intrinsic ... emission intensity with the change in Thioflavin T (ThT) fluores- cent signal under ...
More recently, we also demonstrated that acute ozone exposure results in an increase in the steady- state level of an airway rat-mucinâspecific (ratMUC-5AC;.
Dec 27, 2010 - Xiao Xia Han , Lei Chen , Wei Ji , Yunfei Xie , Bing Zhao , and Yukihiro Ozaki * the target protein. Neither of these techniques showed the.
2Laboratory for Process Technology, Department of Microsystems Engineering. (IMTEK), University of Freiburg, ... 3HSG-IMIT â Institute for Micromachining and Information Technology, ... layout. A master tool is micromilled and then casted with poly
May 4, 2009 - Satyajyoti Senapati,1 Andrew R. Mahon,2 Jason Gordon,3 Carsten Nowak, ..... We would like to thank Matthew Barnes and Sagnik Basuray for ...
saline (PBS) using 36G blunt syringe (NanoFilTM, World .... for rapid delivery, this region would be the best operational zone for low ..... 19 M. S. Kim, K. S. Seo, H. Hyun, S. K. Kim, G. Khang and ... Company, Montvale, NJ, USA, 1998, pp. 2587.
Oct 16, 2008 - This is used for blow molding of thermoplastic foils in order to manufacture lab-on-a-chip cartridges with very thin sidewalls of 100 to 180 µm.
cell detection in biosensors, and HIV monitoring devices, i.e. CD4 counts. Introduction ... chip devices. Examples are: circulating tumor cell detection,1 cell.
Dec 14, 2016 - ceptibility testing are needed because of increasing resistance rates to ..... using Python 2.7.10 (https://www.python.org/), and the packages ...
Feb 12, 2016 - The latter have become extremely rapid via the introduction of MALDI-TOF MASS.[37, 38] It ... Fully auto- mated systems such ... method does not use continual time lapse study, therefore a technician can analyze susceptibil-.
Aug 3, 2012 - the chip was illuminated from its side with a small, blue LED light. (Newark/Element14 .... data analysis, and providing space and time stamps.
Jul 6, 2016 - leukocyte count and C-reactive protein (CRP) level are routinely measured and ... (POC) technologies enabling the quantification of neutrophil ...
www.nature.com/scientificreports
OPEN
received: 05 March 2016 accepted: 16 June 2016 Published: 06 July 2016
Rapid and label-free microfluidic neutrophil purification and phenotyping in diabetes mellitus Han Wei Hou1, Chayakorn Petchakup2, Hui Min Tay1, Zhi Yang Tam1, Rinkoo Dalan1,3, Daniel Ek Kwang Chew1,3, King Ho Holden Li2 & Bernhard O. Boehm1,3,4 Advanced management of dysmetabolic syndromes such as diabetes will benefit from a timely mechanistic insight enabling personalized medicine approaches. Herein, we present a rapid microfluidic neutrophil sorting and functional phenotyping strategy for type 2 diabetes mellitus (T2DM) patients using small blood volumes (fingerprick ~100 μL). The developed inertial microfluidics technology enables single-step neutrophil isolation (>90% purity) without immuno-labeling and sorted neutrophils are used to characterize their rolling behavior on E-selectin, a critical step in leukocyte recruitment during inflammation. The integrated microfluidics testing methodology facilitates high throughput single-cell quantification of neutrophil rolling to detect subtle differences in speed distribution. Higher rolling speed was observed in T2DM patients (P
