Abbott Cell-Dyn 3500 (closed sampler CS and sample loader SL), CD 3700.
tWBC + 5 part differential analysis (reticulocytes optional). Application.
Description.
INTRODUCTION ABBOTT
Reagents for Abbott Counters Abbott Cell-Dyn 3500 (closed sampler CS and sample loader SL), CD 3700 tWBC + 5 part differential analysis (reticulocytes optional)
Rinsing*, on line Reticulocyte count Emergency Cleaning Hematology Control
Blanking Solution ST 1600/2000 Reticount CD ProClean CD CD Diff Control Low CD Diff Control Normal CD Diff Control High
Product Nr. 3976 3825 3839.5000PC 3826 3826.5000 3947 3777 3902.0100PE 3828 3829 3830
Pack size 20 L 5L 5L 20 L 5L 20 L 15 x 3.5 ml 100 ml 3.0 ml 3.0 ml 3.0 ml
ABX
Scatter reagent
Description Diluid ST1600/2000 CyMet 3500 CN Free CyMet 3500 Sheath Fluid 3000/3500
BECKMAN COULTER
Application Diluent Lysing
Abbott Cell-Dyn 3200 tWBC + 5 part differential analysis (reticulocytes optional) Description Diluid Sheath 3200/4000 CyMet 3200 CN Free LeucoLyse Reticount CD ProClean CD CD Diff Control Low CD Diff Control Normal CD Diff Control High
Product Nr. 3832.9020 3823.1000 3827.5000PC 3777 3902.0100PE 3828 3829 3830
Pack size 20 L 1L 5L 15 x 3.5 ml 100 ml 3.0 ml 3.0 ml 3.0 ml
DIATRON
Application Diluent Lysing Scatter reagent Reticulocyte count Emergency Cleaning Hematology Control
Abbott Cell-Dyn 3000 (CS and SL) tWBC + 5 part differential analysis
Emergency Cleaning Hematology Control
ProClean CD CD Diff Control Low CD Diff Control Normal CD Diff Control High
Product Nr. 3976 3824 3826 3826.5000 3902.0100PE 3828 3829 3830
Pack size 20 L 10 L 20 L 5L 100 ml 3.0 ml 3.0 ml 3.0 ml
DREW
Description Diluid ST 1600/2000 CyMet 3000 Sheath Fluid 3000/3500
ERMA
Application Diluent Lysing Scatter reagent
Abbott Cell-Dyn 1300 18p, CD 1300 (CS), CD 1400, CD 1600 (CS),CD 1700 18p,CD 1800, CD 2000 tWBC + 3 part differential analysis
Hematology Control
8 Parameter Control L/N/H
Hematology Control
3 Diff Control L/N/H
Pack size 20 L 1L 5L 20 L 100 ml 1L 5 L Polycube 2.5 ml 4.5 ml 8 ml 2.5 ml 4.5 ml
HYCELL
Product Nr. 3976 3775.1000 3759 3947 3902.0100PE 3917 3917.5000 3724/3725/3726 3701/3702/3703 3721/3722/3723 3734/3735/3736 3820/3821/3822
MEDONIC
Rinsing*, On line Emergency Cleaning
Description Diluid ST 1600/2000 CyMet ST 1600/2000 CyMet ST 1600/2000 CN Free Blanking Solution ST 1600/2000 ProClean CD Hypochlorite 0.5%
15
MINDRAY
Application Diluent Lysing
Abbott Cell-Dyn 400, CD 500, CD 610, CD 700, CD 900 Semi- automated tWBC analysis (non diff) Application Diluent
Description Diluid Azide Free
Lysing
Lyzerglobin Lyzerglobin CN Free
Rinsing, On line Emergency Cleaning
Blanking Solution HgB ProClean Plus Hypochlorite 0.5%
Hematology Control
8 Parameter Control L/N/H
Product Nr. 3957 3958 3956 3769 3077 3850 3851 3946 3901 3917 3917.5000 3724/3725/3726 3701/3702/3703 3721/3722/3723
Pack size 20L 10L 5L 6 x 15 ml 500 ml 6 x 15 ml 500 ml 20L 100 ml 1L 5L Polycube 2.5 ml 4.5 ml 8 ml
Diluid, CyMet and ProClean are all trademarks of Mallinckrodt Baker Inc.
Tips and hints
1. *Blanking Solution ST 1600/2000 is used as on-line cleaner to rinse the WBC counting chambers, RBC Plt counting chambers and both metering tubes. 2. ProClean CD is not advised for use on CD 4000. This analyser should use Hypochlorite solution. 3. CyMet 1600/2000 is not suitable for use on CD 1200, using it will result in incorrect WBC Diff results. CD 1200 are no longer in production. 4. For use on the CD 3200 Leucolyse, nr. 3827.5000PC, is needed to count WBC’s and CyMet 3200, product nr. 3823, to measure the Hemoglobin. Leucolyse, nr 3827.5000PC, has the same application as the Abbott product CD3200 WBC Lyse. CyMet 3200, nr 3823, is equivalent to Abbott product CD3200 HGB/ NOC Lyse.
16
5. J.T.Baker’s calibrator CalSet can be used on CD 1600/1700 to calibrate the standard non diff parameters (WBC, RBC, HgB, MCV, Plt, MPV). It cannot be used to calibrate diff values. Differentials cannot be calibrated on CD 1600/1700. CalSet cannot be used on CD 3200/3500/3700/4000. These instruments use a different measuring principle. 6. 8 Parameter Control available in 2.5 ml, 4.5 ml pierceable and 8 ml vials in three levels. 8 Parameter Control cannot be used on 5 part diff analyzers. It does not work as non-diff control on these analyzers. It is recommended to participate in J.T.Baker’s free external/internal quality assessment program Rapid Stat. It is always recommended, in case of changing reagent type or reagent origin, to check whether a re-calibration is required.
INTRODUCTION 3976
Lyzerglobin or Lyzerglobin Cyanide Free
3769 3077 3850 3851
CyMet ST1600/2000 Cyanide Free CyMet ST1600/2000
3759
ABX CyMet 3500
3839.5000PC
CyMet 3000
3824
Sheath (9.6 L) Cell-Dyn® Reagent 99311-01 Sheath Reagent used to count and differentially classify WBC’s on the CELL-DYN 3000, 3500 and 3700 Hematology Analyzers.
Sheath Fluid 3000/3500
3826
Cell-Dyn® Multi-purpose Detergent Blanking Solution for Hgb channel of semi-automated and automated instruments.
Blanking Solution
3946
Blanking Solution ST 1600/2000
3947
Hemoglobin Lyse (3.8 L) Cell-Dyn® Reagent Hemoglobin Reagent for use on the CELL-DYN 3000 Hematology Analyzers.
Cell-Dyn® Detergent and Cell Dyn Diff Screen Detergent. Blanking Solution for Hgb channel of semi-automated and automated instruments with Diff.
99431-01
99411
99321
Enzymatic Cleaner, 100 ml.
99644
ProClean CD
3902.0100
Diluent/Sheath (20L) Cell-Dyn® Reagent Diluent/Sheath for use on the CELL-DYN 3200
03H79-01 for CD3200
Diluid/Sheath 3200-4000 (20L)
3832.9020
CyMet 3200 CN Free
3823.1000
CD3200 HGB / NOC Lyse (960 ml) Cell-Dyn® Reagent Hemoglobin and Lysing Reagent for use on the CELL-DYN 3200 Hematology Analyzers. CD3200 WBC Lyse (960 ml) Cell-Dyn® Reagent WBC stabilising reagents for use on the CELL-DYN 3200 Hematology Analyzers.
03H80-01
LeucoLyse 3200
3827.5000PC
03H78-01
3H40-01
Reticount CD
3777
Cell-Dyn is a trademark of Abbott
17
MINDRAY
MEDONIC
Reticulocyte Reagent
BECKMAN COULTER
3775.1000
ERMA
Hemoglobin / WIC Lyse (3.8 L) Cell-Dyn® Reagent Hemoglobin and Lysing Reagent for use on the CELL-DYN 3500 Hematology Analyzers. Lytic Reagent also used on CD1300.
99420-01
Diluid ST1600/2000
HYCELL
Lytic Agent (3.8 L) Cell-Dyn® Diff Screen Reagent Lytic agent used to count and size WBC sub-populations on CELL-DYN Diff Screen Analyzers.
Diluid ST1600/2000
J.T.Baker Nr. 3956 3958 3957 3976
DIATRON
Cell-Dyn® Rapid Lyse and Hgb Reagent RBC Lysing and WBC Reagent for semi-automated analyzers.
J.T.Baker Diluid Azide Free
DREW
Abbott Abbott Nr. Diluent (20 L) Diluting Fluid for Semi-Automates and Automates without 3 part Diff. Diluent (20 L) Cell-Dyn® Diff Screen Reagent 99220-01 Diluent for counting and sizing blood cells on all CELL-DYN Diff Screen Analyzers. Diluent (20 L) Cell-Dyn® Reagent 99231-01 Diluent for counting and sizing blood cells on the CELL-DYN 3000, 3500 Hematology Analyzers.
ABBOTT
Cross list: Abbott reagents vs. J.T.Baker
Consumption of reagents for Abbott analyzers
REAGENT CONSUMPTION in ml CELL-DYN 1300
Diluid
Blanking Sol
Lysing
CBC / 3 part diff
35
13
1,2
Start Up / Initialization
15
0
0
Prime / background
85
25
2,5
Daily shutdown
20
22
0
Autoclean
63
35
1
CELL-DYN 1300CS
Diluid
Blanking Sol
Lysing
CBC / 3 part diff
40
13
1,2
Start Up / Initialization
25
0
0
Prime / background
95
25
2,5
Daily shutdown
30
22
0
Autoclean
92
37
1
CELL-DYN 1700
Diluid
Blanking Sol
Lysing
CBC / 3 part diff
36
15
1
Pre-dilute mode
27
15
1,4
Start Up / Initialization
23
0
2,5
Prime / background
62
35
0
Daily shutdown
29
20
0
Autoclean
63
35
1
CELL-DYN 1700CS
Diluid
Blanking Sol
Lysing
CBC / 3 part diff
40
15
1,4
Pre-dilute mode
27
15
1,4
Start Up / Initialization
25
0
2,5
Prime / background
65
35
0
Daily shutdown
40
20
0
Autoclean
92
37
1
Diluid
Sheath
Lysing
Mode
CBC / 5 part Diff
34
13
8
CS
CBC / 5 part Diff
27
13
8
Open
Standby / Prime
300
58
68
Clear orifice
31
0
0
Automatic Clean
20
8
0,5
Autoclean+3 Background
168
73
36
CELL-DYN 3000SL
Diluid
Sheath
Lysing
Mode
CBC / 5 part Diff
32
14
8
SL
CBC / 5 part Diff
27
13
8
Open
Standby / Prime
300
58
68
Clear orifice
31
0
0
Automatic Clean
20
8
0,5
Autoclean+3 Background
168
73
36
CELL-DYN 3000CS Software Revisie U
Software Revisie U
18
INTRODUCTION Consumption of reagents for Abbott analyzers
WBC Lyse
Hgb Lyse
Mode
Background
78
1,2
1
Open
Patient
48
1,1
1
Open
QC
74
1,2
1,2
Open
FWBC
78
1,2
1,1
Open
RRBC
76
1,2
1,1
Open
Change open to closed
11
0
0,1
Background
78
1,2
1
Patient
48
1,1
1
Closed
QC
74
1,2
1,2
Closed
FWBC
78
1,2
1,1
Closed
RRBC
76
1,2
1,1
Closed
Change close to open
17
0
0,2
Prime
232
45,7
44,2
Clean S/V
25
2,3
2,1
Clean Needle
15
0,1
0
Daily Shutdown
0
0,1
0
Auto Clean
414
4,4
3,2
Extended autoclean
199
0,8
0,2
Empty flowcell
43
0,1
0,1
Empty/fill reservoir
197
54
49
CELL-DYN 3200SL Software version 1.8
Diluid
WBC Lyse
Hgb Lyse
Mode
Background
78
1,2
1
Open
Patient
48
1,1
1
Open
QC
74
1,2
1,2
Open
FWBC
78
1,2
1,1
Open
RRBC
76
1,2
1,1
Open
Change open to sampler
9
0,01
0,1
ABX
Diluid
1,2
Sampler
1,3
1,1
Sampler
FWBC
74
1,2
1,1
Sampler
RRBC
77
1,2
1,1
Sampler
Change sampler to open
16
0,2
0
Prime
232
45,7
44,2
Clean S/V
25
2,3
2,1
Clean Needle
15
0,1
0
Daily Shutdown
0
0,1
0
Auto Clean
414
4,4
3,2
Extended autoclean
199
0,8
0,2
Empty flowcell
43
0,1
0,1
Empty / fill reservoir
197
54
49
DIATRON DREW ERMA
1,3
70
HYCELL
37
QC
MEDONIC
Patient
BECKMAN COULTER
Closed
19
MINDRAY
CELL-DYN 3200CS Software version 1.8
ABBOTT
REAGENT CONSUMPTION in ml
REAGENT CONSUMPTION in ml CELL-DYN 3500CS Software REV J 6.1 REAGENT CONSUMPTION in ml
Diluid
Sheath
Blanking Sol.
Lysing
Prime
103
50
62
23
Background
26
14
17
0,8
CBC / 5 part diff
27
11
16
1
CBC extended count
27
11
17
1
Daily shutdown
66
63
0
0
Autoclean + 3 Background.
167
64
100
5
Extended autoclean
134
75
37
2
5 minute cleaning cycle
19,8
9,01
19,82
0
Change open to closed mode Background
25,75
26,04
26,75
0
Change open to closed mode Patient
29,08
16,04
19,42
0,8
Change closed to open mode Background
14,85
10,01
16,85
0
Turn retics package on
0
0
0
0
Retics Mode Patient
9,51
11,82
0
0
Retics Mode Background
8,12
14,42
0
0
Turn retics package off
144,58
26,04
90,17
13,02
Turn vet package on
27,93
15,62
19,62
0,8
Turn vet package off
74,27
29,04
42,61
2
CELL-DYN 3500SL Software REV J 6.1
Diluid
Sheath
Blanking Sol.
Lysing
Prime
103
50
62
23
Background
26
14
17
0,8
CBC / 5 part diff
27
11
16
1
CBC extended count
27
11
17
1
Daily shutdown
66
63
0
0
Autoclean + 3 Background
167
64
100
5
Extended autoclean
134
75
37
2
5 minute cleaning cycle
19,8
9,01
19,82
0 0
Change open to sample loader Background
22,77
10,01
16,85
Change open to sample loader Patient
29,11
10,82
16,25
0,8
Change sample loader to open
16,83
23,03
29,73
0
Turn retics package on
0
0
0
0
Retics Mode Patient
9,51
11,82
0
0
Retics Mode Background
8,12
14,42
0
0
Turn retics package off
144,58
26,04
90,17
13,02
Turn vet package on
27,93
15,62
19,62
0,8
Turn vet package off
74,27
29,04
42,61
2
Cell-Dyn is a trademark of Abbott
Cleaning of Abbott’s automated Cell-Dyn analyzers
As most end-users know, a regular detergent is used on the automated Cell-Dyn analyzers. This detergent is colorless and is meant to clean the hemoglobin flow cell every sample. The CD3000 and CD3200 are exceptions, since these ones don’t require a detergent on-line. The Mallinckrodt Baker on-line detergent is the Blanking Solution ST1600/2000 (3947). What a lot of end-users don’t know is the use of an additional cleaning on the Cell-Dyn analyzers. This cleaning step is a necessity to clean the whole analyzer, preferably once a week. Selecting from the
20
MAIN MENU should activate this cleaning step by proceeding next steps: 1. SPECIAL PROTOCOL 2. MORE 3. AUTO-CLEAN or EXTEND AUTO-CLEAN Follow the steps as displayed on the computer screen. Use for this special cleaning ProClean CD (3902) to fulfill a sufficient cleaning of the analyzer. With this cleaning procedure the whole analyzer is thoroughly cleaned.
INTRODUCTION CD3500, 3700
CD3200
ABX
Product Nr. • 3976 • 3759.1000 or 3759.5000 • 3947 • 3902 • several pack-sizes available • 3976 • 3824 • 3826 • 3902 • 3828, 3829, 3830 • 3976 • 3825 • 3826 • 3947 • 3902 • 3828, 3829, 3830 • 3823.9020 • 3823.1000 • 3826 • 3902 • 3828, 3829, 3830
BECKMAN COULTER
CD3000
Reagents • Diluid ST1600/2000 • CyMet ST1600/2000 CN Free • Blanking Solution ST1600/2000 • ProClean CD • 8 parameter and 3-Diff Control • Diluid ST1600/2000 • CyMet 3000 • Sheath Fluid 3000/3500 • ProClean CD • CD-Diff control • Diluid ST1600/2000 • CyMet 3500 CN Free • Sheath Fluid 3000/3500 • Blanking Solution ST1600/2000 • ProClean CD • CD-Diff control • Diluid/Sheath 3200-4000 • CyMet 3200 CN Free • Sheath Fluid 3000/3500 • ProClean CD • CD-Diff control
DIATRON
Analyzer CD1600, 1700, 2000 CD1300, 1800
ABBOTT
If this additional cleaning step is not done for a long time, the first problem observed is an elevated PLT background after starting up the instrument.
21
MINDRAY
MEDONIC
HYCELL
ERMA
DREW
Diluid, CyMet and ProClean are all trademarks of Mallinckrodt Baker Inc.
Priming and Calibration of Abbott CD Analyzers Abbott CD1300 - CD2000 This priming protocol can be used for several different Celldyns: CD1300, CD1400, CD1600, CD1700 and the CD2000. Priming of Baker reagents as described in the protocol is necessary because these Celldyn instruments have an internal reagent reservoir for the diluent and several syringes filled with Abbott reagents. Before switching from original reagents to Mallinckrodt Baker reagents it is important to know that the instrument is running without any problems and that the values measured for the blood controls (all three levels) are within the specifications of the assay value sheet. Therefore it is necessary to run blood controls or some fresh reference patient samples before you switch to our reagents. These values are necessary for the calibration afterwards. Priming of Cell-Dyn 1300/1400/1600/1700/2000 1. Take the aspirating tubes from the Abbott reagent cubitainers. Don’t lay the tubes on tissues or dusty surfaces. Place them in clean beakers.
2. It is important to rinse the outside surface of the aspirating tubes with distilled or de-ionised water to be aware of any kind of reagent contamination. 3. Press key. 4. Then press key. Press two times . 5. Press to prime isotonic diluent and detergent/blanking solution. 6. Then press . 7.
Put the aspiration tubes into the cubitainers of Blanking Solution ST1600/2000, Diluid ST1600/2000 and CyMet ST1600/2000.
8. Repeat step 5 at least 4 times and step 6 at least 6 times. 9. Press , to shut down the instrument.
22
12. When auto-startup prime cycle and background check are complete, review displayed data. Acceptable values are as follows: Background Count Time: WBC < 0.5 K/µl 4.50 + 0,20 s RBC < 0.05 M/µl 7.00 + 0,20 s HGB < 0.2 g/dL PLT < 10 K/µl 13. When acceptable background values are displayed the priming is successful. If not, repeat point 5 and 6 again. Calibration of Cell-Dyn 1300/1400/1600/1700/2000 FOR CALIBRATION INSTRUCTIONS AND CALCULATING NEW CALIBRATION FACTORS REFER TO THE PARAGRAPH GENERAL INFORMATION.
1.
Choose the option from the MAIN menu. The calibration menu displays the calibration factors for the open mode. Enter new calibration factors by pressing . Enter the new factors by using the numeric keys. Go on to next factor using key . Note: There are CellDyns in the market what have a closed mode system. To change the calibration factors for this closed mode you have to enter a hidden screen. This hidden screen contains so called dilution factors for the open and closed mode. These dilution factors have the same function as calibration factors and are calculated the same way as calibration factors. To enter this hidden screen enter 94043 from the calibration menu. The hidden screen will be accessed and the factors are shown. These dilution factors for the open mode are also very useful when you have calculated a calibration factor that is outside the acceptable range. It is then possible to change the dilution factor instead of the calibration factor. Leave the hidden screen by pressing the key.
10. Press on and then on .
2. Leave the calibration menu by pressing the key. Go to the main menu with the key.
11. The system will be primed and background counting is performed.
3. It’s also possible to run the calibration from the AUTO-CALIBRATE option.
INTRODUCTION
Abbott CD3000/3500/3700
4. The internal reagent reservoirs on the left side of the instrument will be emptied. Be sure that no reagent is left in these reservoirs. 5. DO NOT place the aspiration tubes in the cubitainers with J.T.Baker’s reagents yet. Press on the keyboard . The reagent left in the aspiration tube is aspirated into the reagent reservoirs. 6. The instrument will give a FAULT alarm. Place the aspiration tubes into the cubitainers with J.T.Baker’s reagents. Press . All J.T.Baker’s reagents will be aspirated into the reagent reservoirs. Note that there is some mixing up of Abbott and J.T.Baker reagents in the HGB and SHEATH reservoir. 7. To prevent any problems of contamination in the instrument, empty the internal reagent reservoirs again by pressing . Then fill the reagent reservoirs by pressing . Repeat this step at least 3 times 8. Return to the menu and choose . Perform at least 3 blank counts and check the background values. Acceptable values are:
ABBOTT ABX BECKMAN COULTER
1. Choose from the MAIN menu. The calibration menu displays the calibration factors for the mode indicated. With the functional key you can switch between the open and closed mode calibration factors. Printout the factors for both modes by pressing the PRINT SCREEN key on the KEYBOARD.
DIATRON
Note: The CD3000/3500/3700 use calibration factors and dilution factors. These dilution factors have the same functions as the calibration factors and are calculated the same way. It is possible that the new calculated factor is falling outside the range of the calibration factor. In this case you have to change the dilution factor. If your new calculated calibration factor is outside the range go to step 2 otherwise go to step 3. 2. Press F12 and then F1 on the keyboard. A screen with DILUTION FACTORS for the open and closed mode appears. Enter the new factor for the parameter that has to be calibrated by using the numeric keys. Go to the next factor you want to change by using the arrow keys. When you change the open mode dilution factor it is wise to directly change the closed mode dilution factor again for the parameter you changed. A change in reagents will give the same difference in open and closed mode. Leave the dilution factor screen by pressing the functional key . Go to step 4.
DREW
3. Choose from the main menu of the software program by using the functional keys. Then choose to empty all reagent reservoirs on the CD3000 and press for each separate reagent reservoir on the CD3500 and CD3700.
Calibration of Cell-Dyn 3000/3500 Analyzers FOR CALIBRATION INSTRUCTIONS AND CALCULATING NEW CALIBRATION FACTORS REFER TO THE PARAGRAPH GENERAL INFORMATION.
ERMA
2. Rinse the outside surface of the aspirating tubes with distilled or de-ionised water to be aware of any kind of reagent contamination.
Note: When changing from Cyanide to Cyanide free reagents a recalibration for the Hb is required
3. Choose . You will now see a screen with both the calibration factors for open and closed sampler. Enter the new calculated factor for the parameter that has to be calibrated by using the numeric keys. Go to the next calibration factor you want to change by using the arrow keys. When you change the open mode calibration factor it is wise to directly change the closed mode factor again for the parameter you changed. A change in reagents will give the same difference in open and closed mode.
HYCELL
Priming of Cell-Dyn 3000/3500/3700 1. Take the aspirating tubes from the Abbott reagent cubitainers. Don’t lay the tubes on tissues or dusty surfaces. Place them in clean beakers.
Background: < 0.5 K/µl < 0.05 M/µl < 0.2 g/dL < 10 K/µl
MEDONIC
Before switching from original reagents to J.T.Baker reagents it is important that the instrument is running without any problems and that the values measured for the blood controls (all three levels) are within the specifications of the assay value sheet. Therefore it is necessary to run blood controls or some fresh reference patient samples before you switch to our reagents. These values are necessary for the calibration afterwards.
WBC RBC HGB PLT
4. Leave the calibration menu by pressing key . Go to the main menu with key . 5. It’s also possible to run the calibration from the AUTO-CALIBRATE option
23
MINDRAY
This priming protocol can be used for CD3000, 3500 and 3700. Priming of Baker reagents as described in the protocol is necessary because these Cell-Dyn instruments have internal reagent reservoirs for the reagents and several syringes filled with Abbott reagents.
Abbott CD3200 Before switching from original reagents to J.T.Baker reagents it is important that the instrument is running without any problems and that the values measured for the blood controls (all three levels) are within the specifications of the assay value sheet. Therefore it is necessary to run blood controls or some fresh reference patient samples before you switch to our reagents. These values are necessary for the calibration afterwards. Priming of Cell-Dyn 3200 1. Remove the aspirating tubes from the Abbott reagent cubitainers. Don’t lay the tubes on tissues or dusty surfaces. Place them in clean beakers.
2. Rinse the outside surface of the aspirating tubes with distilled or de-ionised water to prevent any kind of reagent contamination. 3. Choose the option from the main menu of the software program by using the functional keys. Then choose . Choose the functional key from the reagent you want to replace: Abbott Diluent/Sheath HGB/NOC lyse WBC lyse
= = =
Mallinckrodt Baker Diluent/Sheath 3200/4000 Cymet 3200 Sheath Fluid 3000/3500
For example press to replace the HGB/NOC Lyse for Cymet 3200. 4. The Special Protocol function will be activated and it will take some time to empty the internal reagent reservoirs or the reagent lines from the reagent selected. Wait until the special protocol has finished and the function key toggles to . 5. Place the aspiration tube in the cubitainer with J.T.Baker reagent. Press on the keyboard . The selected reagent will be aspirated into the reagent reservoir or into the reagent lines. REPEAT STEPS 3 TO 5 FOR ALL THE THREE REAGENTS CONNECTED!!
6. The reagent reservoirs and lines are now filled with J.T.Baker reagents. But there are still some Abbott reagents left in the flow panel tubings and the syringes! To remove this we choose the option from the main menu. Then choose and . A shutdown cycle will be initiated. When the shutdown cycle is finished the message STANDBY will be displayed in the Status Box. 7. When the instrument is in Standby press the functional key . The instrument will perform a Prime cycle and will come into the READY state. 8. Perform at least 3 blank counts and check the background values. Acceptable values for the Background are:
24
WBC RBC
< 0.5 K/µl HGB < 0.05 M/µl PLT
< 0.2 g/dL < 10 K/µl
Note: When changing from Abbott reagents to J.T.Baker reagents a recalibration for the hgb is required. Calibration of Cell-Dyn 3200 Analyzers FOR CALIBRATION INSTRUCTIONS AND CALCULATING NEW CALIBRATION FACTORS REFER TO THE PARAGRAPH GENERAL INFORMATION.
1. Choose from the MAIN menu. The calibration menu displays the calibration factors for the mode indicated. With the functional key you can switch between the open and closed mode calibration factors. Printout the factors for both modes by pressing the PRINT SCREEN key on the Keyboard. Note: For the CD3200 it is wise not to change the calibration factor for the HGB but the dilution factor. It is possible that the HGB shows a difference from more than 1.5 mmol/L or 2.4 g/dL. The dilution factors have the same functions as the calibration factors and are calculated in the same way. It is possible that the new calculated factor is outside the range of the calibration factor. In this case you have to change the dilution factor. If your new calculated calibration factor is outside the range go to step 2 otherwise go to step 3. 2. Press F12 and then F1 on the keyboard. A screen with DILUTION FACTORS for the open and closed mode appears. Enter the new calculated factor for the parameter that has to be calibrated by using the numeric keys. Go to the next factor you want to change by using the arrow keys. When you change the open mode dilution factor it is wise to directly change the closed mode dilution factor again for the parameter you changed. A change in reagents will give the same difference in open and closed mode. Leave the dilution factor screen by pressing the functional key . Go to step 4. 3. Choose . You will now see a screen with both the calibration factors for open and closed sampler. Enter the new factor for the parameter that has to be calibrated by using the numeric keys. Go to the next calibration factor you want to change by using the arrow keys. When you change the open mode calibration factor it is wise to directly change the closed mode factor again for the parameter you changed. A change in reagents will give the same difference in open and closed mode. 4. Leave the calibration menu by pressing key . Go to the main menu by pressing the functional key . 5. It’s also possible to run the calibration from the AUTOCALIBRATE option. Refer to the operator manual for this procedure.
