Relative microRNA levels Relative mRNA levels - PLOS

1 downloads 0 Views 63KB Size Report
Mmp14. Tro. Eln. Fbn2. Relative mRNA levels. 0.01. 0.05. 0.10. 0.50. 1.00. 5.00. 10.00. 50.00. *. *. *. *. *. **. *. *. B. Figure S1: Validation of differential expression ...
A **

Relative microRNA levels

20

10

5

** *

*

2

1 miR−29a

miR−195

miR−497

miR−101

miR−133a

B Relative mRNA levels

50.00

*

*

10.00 5.00

1.00 0.50

*

*

*

**

0.10

*

0.05

* 0.01 Retn

Rgs2

Tgfb1

Fbn1

Col1a1

Fn1

Fbln2

Mmp14

Tro

Eln

Fbn2

Figure S1: Validation of differential expression of selected miRNAs (A) and mRNAs (B) by qPCR. The fold change of expression in the aortic samples of six-week old mice compared to that of neonatal mice is shown. Data are presented as mean ± SEM (n = 3). Data was normalized to one RNA sample of the neonatal mice, i.e. 1.0. The mean expression of U6 RNA and snoRNA202 served as endogenous control in microRNA analyses, and Actb as control for gene expression analyses. ∗p < 0.05, ∗ ∗ p < 0.01.

1