rev-supp-fig 1 - PLOS

The Sec24D primary structure is highly conserved during evolution H. M. T. D.

sapiens musculus rubripes rerio

MSQQGYVATPPYSQPQPGIG---------LSPPHYGHYGDPS--HTASPTGMMKPAGPLG MSQQGYVATPPYSQSQPGMG---------ISPPHYGHYGDPS--HASSPPGVMKPLGPSMSQQGYVAAPPYSQAQPRMGGYQGGFGPGPAQPMNGHYGGPPQAFTAPPTGMMKPP---MSQQGYVATPPYSQAQAGMGGYAAGFGNPPPQSHYGAYGSPTQGYSAPPTGVLKPP---********:*****.*. :* . . * **.*. .::.*.*::**

49 48 56 56

H. M. T. D.


ATATRGMLPPGPPPPGPHQFGQNGAHATGHPPQRFPGPPPVNNVASSHAPYQPSAQSSYP -AAPSGMLPPGPLPPGPPQFGPNGAHTPGHPPQRFPGPPPVNSVAPSYASGQTLPQSSYP -VSS-GAMPP---PP--SQFSPN-MQHNG--PQSYP------AASAAYGQPP---YNSMA -ASSPSGMPP---PPVSSQYGTN-IQQNGAHPHSFPPPVSSSPSMSPYGQPPPTAFHSMA .:. . :** ** *:. * : * *: :* ..:. * .

109 107 97 111

H. M. T. D.


GPISTSSVTQLGSQLSAMQINSYGSGMAPPSQGPPGPLSATSLQTPPRPPQPSILQPGSQ GPGSTSSVTQLGSQFSSMQINSYGVGATPQSQGPPGPQSAGAFQGPPQPAQPSILQPGHQ S-QAPPPTQHLTNQMSAMNLGSYVMGQP---QMPPGPQSP--QMSPPQSPSASMPMGGPP --QAPPPTQQLTNQMSAMNIAGYGQRPV---QSPSNSAPA--HFQTSPPP---PVMGHPP :.... :* .*:*:*:: .* * *... .. .. ..

169 167 151 161

H. M. T. D.


VLPPPPTTLNGPGASPLPLPMYRPDGLSGPPPPNAQYQPPPLPGQTLGAGYPPQQAN--VPPPPPTALNGPGASPMSPPTHRQDGLPGPAPLNAQYQPPPPPGQTLGPGYPPQQATN-MAGPPMAGPPMAG-PPMAGMG-RP--FPGPSPPGPGGFPQPSPGTAVPPGYPQQPGQ--LSPPGQQPSPLGSPPPMATMGSMP--APPMGMSGPPGPVVHQP-PHGPQGYPQQPGSPIA : * . .*:. . .. * *** * .

226 225 204 218

H. M. T. D.


--SGPQMAGAQLS------YPGGFPGGPAQMAGP-PQPQKKLDPDSIPSPIQVIENDRAS --YGPQMGGAQMS------YPGGFPGGPAQMAGPAPQLQRKLDPDSIPSPIQVIENDRAT --FGGLIAGPQQG------MPGAFPGAPGGLAGP---PQKKLDPDSIPSTNRCVWQTQXD GPYGAQMAGPQMAGPQTGAFPGGFAGGPAQMAGP---PQKKLDPDSIPSTTQMIADDQAK * :.*.* . **.*.*.*. :*** *:*********. : : : :

277 277 253 275

H. M. T. D.


RG-------GQVYATNTRGQIPPLVTTDCMIQDQGNASPRFIRCTTYCFPCTSDMAKQAQ RG-------GQVYTTNTRGQVPPLVTTECVIQDQGHSSPRYIRCTTYCFPCTSDMAKQAQ RGRPDQNTGGRSKTTNIRGQVPPLVTTDFTVQDQGNASPRYMRCTAYSLPTTADLAKQCQ RG-------GQVYVTNIRGQVPPLVTTNFTVQDQGNASPRFMRCTTYSFPCTADLAKQCK ** *: .** ***:******: :****::***::***:*.:* *:*:***.:

330 330 313 328

H. M. T. D.


IPLAAVIKPFATIPSNESPLYLVNHGESGPVRCNRCKAYMCPFMQFIEGGRRYQCGFCNC IPLAAVIKPFADIPPNETPLYLVNHGESGPVRCNRCKAYMCPFMQFIEGGRRYQCGFCSC VPLATIITPLAALPKNEAPLYLVNHGETGPIRCNRCKAYMCPYMQFTDGGRRYQCSFCNC VPLAAIIKPFATVPKNETPLYVVNHGETGPIRCNRCKAYMCPYMQFIDGGRRFQCSFCSC :***::*.*:* :* **:***:*****:**:***********:*** :****:**.**.*

390 390 373 388

H. M. T. D.


VNDVPPFYFQHLDHIGRRLDHYEKPELSLGSYEYVATLDYCRKSKPPNPPAFIFMIDVSY VNEVPPFYFQHLDHIGRRLDHYEKPELSLGSYEYVATLDYCRKNKPPSPPAFIFMIDVSY VNEVPVFYFQHLDHMGRRVDFYERPELSLGSYEFVATLDYCKNNKPPNPPAYIFMIDVSY VNEVPVHYFQHLDHMGRRMDLYERPELSLGSYEFAATLDYCKNNKPPNPPAYIFMIDVSY **:** .*******:***:* **:*********:.******::.***.***:********

450 450 433 448

H. M. T. D.


SNIKNGLVKLICEELKTMLEKIPKEEQEETSAIRVGFITYNKVLHFFNVKSNLAQPQMMV SNIKNGLVKLICEELKTALKRLPKEEHEETSAIRVGFITYNKVLHFFNVKSNLAQPQMMV NNVKSGLVKLICEELKTLLQNLPREDGADASAIKVGFVTYNKILHFYNVKSALAQPQMMV NNIKSGLVRLICEELKTLLDRLPKEEGAESSSIKVGFVTYNKILHFYNVKSALAQPQMMV .*:*.***:******** *..:*:*: ::*:*:***:****:***:**** ********

510 510 493 508

H. M. T. D.


VTDVGEVFVPLLDGFLVNYQESQSVIHNLLDQIPDMFADSNENETVFASVIQAGMEALKA VTDVGEVFVPLLDGFLVNYEESQSVIHNLLDQIPEMFADSNENETVFAPVIQAGMEALKA VSDTAEMFVPLQDGFLVSYQESRAVISNLLDQIPDMFADTGESETVFAPVIQAGIEAFKA VSDTAEMFVPLLDGFLVNFQESRAVINNLLDQIPDMFADTNESETVFAPVVQAGLEALKA *:*..*:**** *****.::**::** *******:****:.*.*****.*:***:**:**

570 570 553 568

H. M. T. D.


ADCPGKLFIFHSSLPTAEAPGKLKNRDDKKLVNTDKEKILFQPQTNVYDSLAKDCVAHGC AECPGKLFIFHSSLPTAEAPGKLKNRDDKKLVNTDKEKILFQPQTAVYESLAKDCVANSC AQCSGKLFIFHSSMPTAEAPGKLKNRDDKKLVGTDKEKTLFQPQKGVYEQLSKECVAQGC AECSGKLFIFHSSIPTAEAPGKLKNRDDKKLVGTEKEKTLFQPQRGVYEQLTKDCVAQGC *:*.*********:******************.*:*** ***** **:.*:*:***:.*

630 630 613 628

H. sapiens M. musculus T. rubripes

SVTLFLFPSQYVDVASLGLVPQLTGGTLYKYNNFQMHLDRQQFLNDLRNDIEKKIGFDAI SVTLFLFPSQFVDVASLGLVPLLTGGTLYKYNVFQIHSDSQRFLTDLRNDIEKKIGFDAI CVDLFLFPSQYVDVSTMGDVPARTGGSVYKYSNFQVDVDGEHFLRDLRKDVQKRVGFDAI

690 690 673

D. rerio

CVDLFLFPNQYVDIATMGDVPSHTGGSIYKYSNFQVEVNGQQFLSDLSRDVEKSIGFDAI .* *****.*:**::::* ** ***::***. **:. : ::** ** .*::* :*****

687

H. M. T. D.


MRVRTSTGFRATDFFGGILMNNTTDVEMAAIDCDKAVTVEFKHDDKLSEDSGALIQCAVL MRVRTSTGFRATDFFGGIFMNNTTDVEMAAIDCDKAVTVEFKHDDKLSEDVGALIQCAVL MRVRTSTGFRATDFFGAIHMNNTTDVEMAAVDCDKAVTVELKHDDALNEEAGALLQCALL MRVRTGTGFRATDFFGAVYMNNTTDVEMAAVDCDKAVTVEFKHDDTLSEESGAVMQCALL *****.**********.: ***********:*********:**** *.*: **::***:*

750 750 733 748

H. M. T. D.


YTTISGQRRLRIHNLGLNCSSQLADLYKSCETDALINFFAKSAFKAVLHQPLKVIREILV YTTISGQRRLRIHNLALNCSTQLADLYKSCETDALINFFAKSAFKAVLNQPLKAIREILV YTTIGGQRRLRVHNLSLNCSSQLSELYKSCETDALINFFAKSAYRAILNQPLKSVREILV YTTIGGQRRLRIHNLSLNCSSQLSELYKSCETDALINFFAKSAYRAMLNQPMKTVREILV ****.******:***.****:**::******************::*:*:**:* :*****

810 810 793 808

H. M. T. D.


NQTAHMLACYRKNCASPSAASQLILPDSMKVLPVYMNCLLKNCVLLSRPEISTDERAYQR NQTAHMLACYRKHCASPSAASQLILPDSMKVLPVYMNSLLKNCVLLSRSEISPDERAYQR NQTAHMLACYRKNCASPSAASQLILPDAMKVFPVYMNSLMKTGPLVGSTELSTDDRAHQR NQTAHMLACYRKNCASPSAVSQLILPDAMKVFPVYMNSLLKTPALVSSTELSTDDRAFHR ************:******.*******:***:*****.*:*. *:. .*:*.*:**.:*

870 870 853 868

H. M. T. D.


QLVMTMGVADSQLFFYPQLLPIHTLDVKSTMLPAAVRCSESRLSEEGIFLLANGLHMFLW QLVMTMGVADSQLFFYPLLLPIHTLDVKSAALPPAVRCSESRLSEEGIFLLANGLNMFLW LAVMAMGVEETQLLLYPRLIPLHNMDVGAEAPPTPLRCSEERLADAGVFLLENGHAMFLW FMVNAMGVEETQVLLYPRLIPLHTMDVSSESLPAAVRCSEERLNESGMFLLENGLSMFLW --* :*** ::*:::** *:*:*.:** : *..:****.** : *:*** ** ****

930 930 913 928

H. M. T. D.


LGVSSPPELIQGIFNVPSFAHINTDMTLLPEVGNPYSQQLRMIMGIIQQKRPYSMKLTIV FGVGSPPELIQGIFNVPSFAHINTDMASLPEVGSPHSQQLRMIMNNIQQKKPYSMKLIVV LGQASPPDLIQNLFNVPSLAHLQAHMSVLPVLDNPLSKKVRGVIGDLLEKRPGSMKLQIV LGQACSPDLIQNLFNVPSFGHLPTEGTSLPVLDNAHSRKLHSIISRISQQRAASMKLLIV :* ...*:***.:*****:.*: :. : ** :... *:::: ::. : :::. **** :*

988 990 971 988

H. M. T. D.


KQREQPEMVFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN KQREQREMAFRQFLVEDKGLYGGSSYVDFLCCVHKEICQLLN RQKDKPEMLFRQFLVEDKGLHGGASYMDFLCYIHREVRQLLKQKDRAEMSFRQLLVEDKGLHGGASYMDFLCFVHREIRQLLT :*::: ** ***:*******:**:**:**** :*:*: ***

Sarmah et al., Figure S1

1032 1032 1014 1030