Revised Supp Figures Paggetti et al

Supplemental Figure 1

HEK293T t pu n I

rl Ct

IP

M

O

Z

MLL-N

250 kDa

150 kDa

b SRC-3 t(8;20)(p11;q13) p300 t(8;22)(p11;q13) TIF2 inv(8)(p11q13) MOZ

NEMM PHD PHD

MYST

ED

CBP t(8;16)(p11;p13)

SM

P 2004

N352

NEMM PHD PHD

N760

NEMM PHDPHD

C1409

MYST SM

P

Supplemental Figure 2

HEK293T

Dilution of Input

1

1:2

1:4

1:8

HOXA5 HOXA7 HOXA9

CD34+

Dilution of Input

1 HOXA5 HOXA7 HOXA9

1:2

1:4

1:8

Supplemental Figure 3 a CD34+

Mll

mRNA relative expression

1

CD34+ siRNA

0.8

Luc MOZ MLL MLL

170 kDa

0.6

130 kDa

0.4 0.2

0 siRNA

Ponceau

Luc

MLL

MOZ

b Moz

CD34+

mRNA relative expression

1

CD34+ siRNA

Luc

MLL

MOZ

250 kDa

0.8 150 kDa

MOZ

0.6 0.4 0.2

0 siRNA

Ponceau

Luc

MOZ

MLL

Supplemental Figure 4 a CD34+ siRNA

t pu n I

rl Ct

M

O

Z M

-C LL

t pu n I

rl Ct

M

O

Z M

-C LL

t pu n I

rl Ct

M

O

Z M

Luc MLL MOZ 1

2

3

4

1

2

HOXA5

b

t pu In

rl Ct

1

4

HOXA7

CD34+ siRNA

3

M

O

Z M

-C LL

2

3

HOXA9

4 K 1 16 4 9 K 3 K 3K t H4 pu trl e 3H cH e 2H c n C A I M A M

Luc

MLL

MOZ

GAPDH

4 3K

4

-C LL