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100. % of CD8. +. Tcells. CD8+ T cells. CD4+ T cells. 17.6. 75.0. 0. -10 3. 10 3. 10 4. 10 5. 0. -10 3. 10 3. 10 4. 10 5. 52.1. 42.2. 0. -10 3. 10 3. 10 4. 10 5. 0. -10 3.

A

CD4+ T cells d0 10

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S1 Fig: CD62L and CD44 expression profiles of T cells during the course of L. monocytogenes infection Wildtype mice were i.v. infected with 1×105 LmOVA. At different time points post infection, spleen cells were analyzed by flow cytometry and changes in expression profile as well as percentages and numbers of different T-cell subsets were determined. (A) Representative dot plots for CD4 and CD8 gated cells. (B) Percentages of naive CD62L+CD44— and activated CD62L—CD44+ CD4+ and CD8+ T cells. Values give the mean ± SEM for 3 independently analyzed mice per time point and are representative for 3 independent experiments. (C, D) Numbers of total, CD62L+CD44—, and CD62L—CD44+ cells for CD39+ (C) and CD73+ (D) CD4+ and CD8+ T cells. Values give the mean ± SEM for 6 mice per group pooled from 2 independent experiments. Unpaired t test, comparison with day 0 (only shown for total T-cell populations), * p