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Comment

C. B. Setdb11lox/+/Zp3-Cre+. Zp3-Cre+. Setdb11lox/+. Setdb1 KO. Control. Zp3-Cre+. Zp3-Cre+. Setdb12lox/2lox. Setdb12lox/+. Setdb12lox/1lox. Setdb12lox/+.

A

ATG 1

Setdb1 locus

TAG

2

16 F1

Targeted allele (Setdb13lox)

F2 R1

1

2

16

Condi>onal allele 1 (Setdb12lox)

2

16 F1

Null allele (Setdb11lox)

1

22 R1

Zp3-Cre+

Setdb12lox/2lox

Setdb11lox/+/Zp3-Cre+

Setdb12lox/+ Zp3-Cre-

Setdb12lox/1lox Zp3-Cre-

Setdb12lox/+ Zp3-Cre+

200-

Marker

Control

bp 850- 650- 500- 400- 300-

22

2

Setdb11lox/+

C

Setdb12lox/1lox Zp3-Cre+ Setdb1 KO

2lox/+ 2lox/1lox 2lox/+ 2lox/1lox Cre- Cre- Cre+ Cre+ 1

22

IRES-‐βGeo

F2 R1

F1

B

22

2

3

4

5

6

7

8 1lox (478 bp) 2lox (403 bp) Cre (367 bp) WT (249 bp)