Seasonal variability of intestinal microbiota in rainbow trout - Revue de ...

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to streptomycin, oxytetracycine, chloramphenicol and florfenicol. In conclu- sion, Aeromonas spp. are easy to isolate, and naturally susceptible to the.

Seasonal variability of intestinal microbiota in rainbow trout (Oncorhynchus mykiss), with a particular attention to Aeromonas spp. as candidate indicator of antimicrobial resistance M. NAVINER*, E. GIRAUD, H. LE BRIS, F. ARMAND, C. MANGION AND J.-P. GANIÈRE Unité Mixte de Recherche INRA-ENVN Chimiothérapie Aquacole et Environnement, Ecole Nationale Vétérinaire, BP 40706, 44307 NANTES Cedex 03, France * Corresponding author : Email: [email protected]



The objectives of this work were (1) to study the importance of Aeromonas bacterial genus in gut microbiota of farmed trout in relation with seasonal variability and (2) to evaluate the relevance of Aeromonas as bacterial indicator for antimicrobial resistance emergence in freshwater fish farming. The quantitative and qualitative bacteriological composition of trout gut microbiota was examined over a one-year period with regular sampling of five fish in a freshwater farm in Brittany, France. The composition of culturable flora was assessed by counts on selective and non-selective media. Gram-negative bacteria, in particular Aeromonas spp. isolates, were identified by cultural, biochemical and PCR analysis methods. Important variations were observed throughout the year, in both the quantitative (

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