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Loxapine. These drugs and metaboiftes either were not dansylated, were not extracted, or were eluted with the solvent front. fluorescence detection method (8) ...
CLIN. CHEM.

38/9, 1756-1761

(1992)

Sensitive and Selective Liquid-ChromatographicAssay of Fluoxetine and Norfluoxetine in Plasma with Fluorescence Detection after Precolumn Derivatization Raymond

F. Suckow,”2

Ming Fen Zhang,2 and Thomas

We determined fluoxetine (Prozac#{174}) and its major metabolite norfluoxetine in plasma by liquid chromatography with fluorescence detection. After liquid-liquid extraction from 1 mL of plasma, the extract was denvatized at room temperature with dansyl chloride, and the highly fluorescent derivatives were chromatographed with a reversedphase C15 column and a mobile phase of phosphate buffer and acetonitrile. Dansylated fluoxetine, norfluoxetme, and the internal standard were eluted in