Loxapine. These drugs and metaboiftes either were not dansylated, were not extracted, or were eluted with the solvent front. fluorescence detection method (8) ...
CLIN. CHEM.
38/9, 1756-1761
(1992)
Sensitive and Selective Liquid-ChromatographicAssay of Fluoxetine and Norfluoxetine in Plasma with Fluorescence Detection after Precolumn Derivatization Raymond
F. Suckow,”2
Ming Fen Zhang,2 and Thomas
We determined fluoxetine (Prozac#{174}) and its major metabolite norfluoxetine in plasma by liquid chromatography with fluorescence detection. After liquid-liquid extraction from 1 mL of plasma, the extract was denvatized at room temperature with dansyl chloride, and the highly fluorescent derivatives were chromatographed with a reversedphase C15 column and a mobile phase of phosphate buffer and acetonitrile. Dansylated fluoxetine, norfluoxetme, and the internal standard were eluted in