Skin Fibroblasts of

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Effect of Low-Level Laser Therapy on Skin Fibroblasts of ... densities were applied four times to each organ culture and cell culture. The organ cultures were ...

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wererble to obtaincul(uresol'tibroblast-likecells,Therewere stainedwith urirnylacetateundleadcitrate,aildexaminedwith l0 wells for each laser-treated cell culture and its matclring a ZeissEM 900 transrnission elechonmicroscope(Zeiss,Gersham-exposed cell culture.Therewere a totnl of 20 wells for many)at 80 kV. The grids of sham-exposed and laser-trented eachanirnal.TherewereaboutI X 105fibroblastsin eachl00 l'ibroblasts werethen unulyzett. ,L of ccll culturenrecliunr. Cclls in thc wells of thc shant-exposedculluresilndtheirltlser-tleated courrtcrparts werecountcd Statistic'uldildb,sis by Neubauer slide,We wereobleto controltbenumberof cells 'Ihe uonlarametricWilcoxontestwts usetllirr comparison in cachwell andnorrnalizecl thc shnnr-cxposcd andlascr'-tr.eatccl of hishrlogiculstudyrrrrl cell prolifcrationlss.rydttr. Statisticultures, cnl signil'iclncel'ur nll testswns sct ot /, = 0.05, Low-l evcl luter tlwrtqt.y Thc ccllsin thc l0 rvellsof enchltser-trentcltl cultulewcre then irra

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