Supplementary Material Soil fungal:bacterial ratios are linked to altered carbon cycling Ashish A. Malik*, Somak Chowdhury, Veronika Schlager, Anna Oliver, Jeremy Puissant, Perla G. Mellado Vazquez, Nico Jehmlich, Martin von Bergen, Robert I. Griffiths, Gerd Gleixner * Correspondence:
[email protected]
Supplementary Material Supplementary Figures Figure S-1: Organic C, N and C:N ratio of the soils (0-10 cm depth) used in the experiment. 2.5
Soil C [%]
2 1.5 1 Low F:B 0.5 0 2000
High F:B
2002
2004
2006 Year
2008
2010
2012
0.25
Soil N [%]
0.2 0.15 0.1
Low F:B 0.05
0 2000
High F:B
2002
2004
2006 Year
2008
2010
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12
Soil C:N Ratio
10 8 Low F:B
6
4 2000
High F:B
2002
2004
2006 Year
2008
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2
Figure S-2: Relative abundance of the major phyla in the two soils and the shifts on litter addition obtained using RNA-Seq based taxonomic classification
0
10
Abundance [%] 20
30
Low F:B Litter -
40
Actinobacteria Proteobacteria Firmicutes Bacteroidetes Streptophyta Planctomycetes
Low F:B Litter +
Cyanobacteria Ascomycota Verrucomicrobia Chloroflexi Arthropoda Tenericutes Acidobacteria
High F:B Litter -
Thaumarchaeota Aquificae
High F:B Litter +
3
Supplementary Material Figure S-3: RNA-Seq derived relative abundance of bacterial and eukaryotic domains in the two soils and their shift on litter addition. Global effect of F:B ratio and litter addition was assessed by repeated measures ANOVA.
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Figure S-4: RNA-Seq derived relative abundance of major fungal phyla in the two soils and their shift on litter addition. Global effect of F:B ratio and litter addition was assessed by repeated measures ANOVA.
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Supplementary Material Figure S-5: Actinobacteria: rest of bacteria (A:RB) ratio estimated using RNA-Seq. Error bars represent standard error (n=3), lowercase letters from Fischer LSD test; treatments sharing a letter are not significant.
1
b
A:RB Ratio
0.8 0.6
a
a
a
0.4 0.2 0 0d Litter-
7d Litter+
Low F:B Soil
0d Litter-
7d Litter+
High F:B Soil
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Figure S-6: Temporal trend in the rate microbial respiration. Note: error bars represent standard error; n=3. 4 Low F:B Soil
Rate of microbial respiration [µgC/g/h]
High F:B Soil
3
2
1
0 0
5
10
15
20
25
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Time after litter addition [d]
Figure S-7: δ13C excess in microbial respired CO2 and its temporal trend over the first 24h after litter addition. Note: error bars represent standard error; n=3.
δ13C respired CO2 [‰]
500 400 300 200 Low F:B
High F:B
100 0 0
4
8
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16
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24
Time after litter addition [h]
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Supplementary Material Figure S-8: Shifts in abundance of different COG/KOG functional groups after litter addition at different time points obtained using: (A) RNA-Seq: error bars represent standard error (n=3), and (B) Proteomics, values were derived from composite samples of replicates at each time point. (A)
Fold change in relative abundance of transcripts 0.8
1
(B) 0
1.2
Carbohydrate transport and metabolism
Fold change in relative abundance of proteins 1 2
Carbohydrate transport and metabolism
Energy production and conversion Amino acid transport and metabolism
Energy production and conversion
Lipid transport and metabolism Amino acid transport and metabolism
Replication, transcription and translation Low F:B 7 d
High F:B 7 d Lipid transport and metabolism
Replication, transcription and translation Low F:B High F:B
1d 1d
7d 7d
30 d 30 d
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