NADPH thioredoxin reductase acclimated to various photoperiods. Anna Lepistö ... Primers and description of yeast two-hybrid assay. The list includes a ...
Starch and antioxidant metabolism in Arabidopsis knockout mutant of chloroplast NADPH thioredoxin reductase acclimated to various photoperiods Anna Lepistö, Eveliina Pakula , Jouni Toivola , Anja Krieger-Liszkay, Florence Vignols, Eevi Rintamäki
Supplementary Table S1. Rosette dry weights in Arabidopsis thaliana Col-0 and ntrc grown under various light-dark rhythms for equal hours of light.
Light (L)/
Total hours and
dark (D) rhythm
light / dark
Rosette
Biomass
ntrc
Rosette dry
biomass,
hours in the day
weight,
% of
of harvest
mg
Col-0
age, d
(in parenthesis) 16L / 8D
12L / 12D
14L / 14D
264 (176 / 88)
336 (168 / 168)
360 (180 / 180)
11
14
15
= T28 8L / 16D
528 (176 / 352)
22
Col-0
2.8 ± 0.2c
ntrc
1.6 ± 0.1b
Col-0
4.6 ± 0.4c
ntrc
1.5 ± 0.2b
Col-0
2.9 ± 0.4c
ntrc
0.7 ± 0.04a
Col-0
4.3 ± 0.7c
ntrc
0.4 ± 0.04a
57%
33%
24%
9%
Rosettes were harvested at stage, in which the plants were exposed to circa equal light hours (168 - 180 h). Rosette age (d) is the number of T24 cycles from germination to harvesting. Biomass data are means ± SE, n=5 in all experiments. For phenotype of plants, see Supplementary Figure S1. Statistically significant differences (ANOVA, Tukey's HSD; P
