Sep 15, 2015 - cross-linking (Schwarzbauer and DeSimone, 2011). FN also plays an indispensable role during development, wound healing, and.
MBoC | ARTICLE
Studying early stages of fibronectin fibrillogenesis in living cells by atomic force microscopy Tetyana Gudzenko and Clemens M. Franz DFG–Center for Functional Nanostructures, Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany
ABSTRACT Fibronectin (FN) is an extracellular matrix protein that can be assembled by cells into large fibrillar networks, but the dynamics of FN remodeling and the transition through intermediate fibrillar stages are incompletely understood. Here we used a combination of fluorescence microscopy and time-lapse atomic force microscopy (AFM) to visualize initial stages of FN fibrillogenesis in living fibroblasts at high resolution. Initial FN nanofibrils form within
