Superoxide Dismutase and Catalase Conjugated

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increasing the molecular mass of superoxide dismutase from. 32 kDa to about 100 kDa for the modified protein. Similarly, the molecular weight of catalase may ...

THEJOURNAL OF BIOLOGICAL CHEMISTRY 0 1988 by The American Society for Biochemistry and Molecular Biology, Inc

Vol. 263, No. 14, Issue of May 15, pp. 6884-6892, 1988 Printed in U.S.A.

Superoxide Dismutase and Catalase Conjugated to Polyethylene Glycol Increases Endothelial Enzyme Activityand Oxidant Resistance* (Received for publication, September 3, 1987)

Joseph S. Beckman$, RobertL. Minor, Jr.5, Carl W. Whiten, John E. Repinell, Gerald M. Rosenil, and BruceA. Freeman$ From the $Departments of Anesthesiology and Biochemistry, University of Alabama at Birmingham, University Station, Birmingham, Alabama 35233, the §School of Medicine, University of Iowa, Iowa City, Iowa 52242, the ll Webb-Waring Lung Institute, University of Colorado Health Sources Center, Denver, Colorado 80302, and the 11 Department of Pharmacology, Duke University Medical Center, Durham, North Carolina 27710

Covalent conjugation of superoxide dismutase and catalase with polyethyleneglycol (PEG) increases the circulatory half-lives of these enzymes from