Supp fig 1 - PLOS

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McIntyre et al., Supp Fig. 1. B. Neg. control. Ladder. +/+ Tírc +/- Tírc -/-. +/+. +/- Tfrc. -- Tfro. Tfrc. Tfrc. Tfro. Cenpj. Cenpj. Cenpj. MBKA60.3c WT (used as.
McIntyre et al., Supp Fig. 1

A

B

Neg. control Ladder

C +/+

+/-

Tfrc

Cenpj

-/-

Tfrc

Tfrc

Cenpj Cenpj

MBKA60.3c WT (used as calibrator). Cenpj and Tfrc endogenous control Ct ~ 1 cycle apart

MBKA69.1c heterozygote. Cenpj and Tfrc endogenous control Ct ~ 2 cycles apart

MBKA69.1e homozygote. No amplification of Cenpj assay above the Ct detection threshold

D PCR Vector Backbone (VF4) PCR Cenpj WT PCR Cenpj Mut PCR LacZ PCR 5' FRT PCR LoxP PCR WT LoA qPCR

F PRIMER GTAGCTGACATTCATCCGGG TCAAGCTATTTTGGCTCCACAG TCAAGCTATTTTGGCTCCACAG ATCACGACGCGCTGTATC AGGCGCATAACGATACCACGAT ATCCGGGGGTACCGCGTCGAG GCAGAAGGTGTCCAGTACAATGT

R PRIMER CTTTTCTACGGGGTCTGACG TTGATGATTCCCAGCACCAC TCGTGGTATCGTTATGCGCC ACATCGGGCAAATAATATCG CCACAACGGGTTCTTCTGTT ACTGATGGCGAGCTCAGACC GCAAGAGACTGTGGTTGGAAGTAAG