Suppl figures Oncogene (Final ppt 06 Mar2015) - Nature

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p75. i.p w/ PDLIM1 Ab input. kDa. CTF full-length. 19. PDLIM1. 25. ICD. i.b w/ PDLIM1 Ab. Supplementary figure S7. kDa p75. PDLIM1 actin input. i.p w/ p75 Ab.
Migratory Index (% of untreated Control)

Supplementary figure S1

140 120 100 80 60 40

*!

20

**!

0 U87R

U87R + Chol Ox

U87R ++! U87R M-β-CD! M-b-CD

MESF/ABC (X103)!

Supplementary figure S2

1000! 900! 800! 700! 600! 500! 400! 300! 200! 100! 0!

**! *!

pcDNA!

p75!

S303G!

Supplementary figure S3

BDNF (pg/ml)!

2500!

Cell lysates!

Supernantants!

*!

2000!

**!

1500! 1000! 500! 0! pcDNA!

p75!

S303G!

Supplementary figure S4

1800!

**!

MESF/ABC (X103)!

1600! 1400!

***!

1200! 1000!

*!

800! 600! 400! 200! 0! pcDNA

p75

SPV ΔSPV!

SPM

Supplementary figure S5

Cell lysates 1600

*!

1400 BDNF (pg/ml)!

Supernatant

1200

***! **!

1000 800 600 400 200 0 pcDNA!

p75!

ΔSPV

SPM!

Supplementary figure S6

Supplementary figure S7

i.p w/ p75 Ab !

input!

i.p w/ PDLIM1 Ab!

input!

kDa! p75!

kDa!

PDLIM1! i.b w/ p75 Ab

75!

full-length!

actin! p75!

75!

full-length!

25! 19!

i.b w/ PDLIM1 Ab

25!

CTF!

CTF! ICD!

actin! PDLIM1!

Supplementary figure S8

i.p w/ PDLIM1 Ab !

input!

i.p w/ PDLIM1 Ab!

kDa!

kDa! p75! 75!

25! 19! i.b w/ PDLIM1 Ab

full-length!

input!

p75!

75!

full-length!

25!

CTF!

CTF! ICD! actin! PDLIM1!

i.b w/ PDLIM1 Ab

actin! PDLIM1!