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BZR1. BZR1-CFP. /WT. BZR1-CFP. /a1a3. Ponceau S e c. Hypocotyl (mm). D. MS BRZ d f g bri1-1. 16 bzr1-1D a1a3 bzr1-1D. Fig. S5. MS BRZ. Hypocotyl (mm).
a!

BR!

b!

BR!

BRI1!

BRI1!

BSKs!

BSKs!

?

?

BSU1(PP1)!

BSU1(PP1)! ?!

PP2A! BIN2(GSK3)!

BIN2(GSK3)! pBZR1/2!

pBZR1/2!

BZR1/2!

BZR1/2!

Gene expression!

Gene expression!

Fig. S1!

a

Protein name

Gene ID

BZR1! RCN1! PP2A A2! PP2A A3! PP2A B" # PP2A B" $* PP2A B" !! 14-3-3 "! BIN2*! AtSK12! AtSK31!

At1g75080! At1g25490! At3g25800! At1g13320! At3g26020! At3g09880! At1g13460! At5g10450! At4g18710! At3g05840! At3g61160!

Unique peptides sequence coverage! IP1 IP2 IP1 IP2! 18! 1! 2! 2! 2! 1! 1!

b

c

Fig. S2.

14! 1! 6! 3! 5! 1! 4! 4! 6! 1!

59%! 2.7%! 2.5%! 2.6%! 6.1%! 2.2%! 2.2%!

54.2%! 2%! 14%! 6.6%! 10.2%! 2.2%! 7.7%! 15.7%! 14.2%! 2.1%!

a

AD-BZR1 A1

A2

A3

B’!

B’"

C1

C2

C5

BD -AH +AH

b

AD-BZR2 B’! B’"

BD -AH +AH

Fig. S3.

!!!!!!WS

c!

B"%-OX!

Fold change

a!

bri1-5 !

WS

2

CPD

d! bri1-5

B"%-OX/bri1-5

!

OX

1 0

b!

bri1-5

DWF4

!!!!!!!!!!!!B" % OX / bri1-116!

e! BL

-

Col!

+P! - P! Stain!

Fig. S4!

+

bri1-116!

B"!-OX/! bin2-1! B"!-OX/! bin2-1!

-

+

-

+

-

+!

*

b"! ! *

* *

3! *

*

*

*

1! BL (nM) 0! ! 0.1! 1!

10! 100! 500 !

*

8!

*

4! 0!

f! 5!

4! 2!

10!

MS

3!

Col BRZ! c5 BRZ! Col! c5! *

2!

**

4!

MS

BRZ!

*

0!

DWF4!

CPD!

Ponceau S -

+

-

Col C5

* MS

BRZ

g!

1!

pBZR1! BZR1!

BL

BRZ!

16 14 12 10 8 6 4 2 0

**

BZR1-CFP! BZR1-CFP! /WT! /a1a3 !

h!

e

D

Col! b"! !

+!

Fig. S5.

bzr1-1D! bzr1-1D bri1-116! bzr1-1D bin2-1! bzr1-1D a1a3!

root (mm)! hypocotyl (mm)!

Col!

d

c

Hypocotyl (mm)

UBC!

b 12! 10 ! 8! 6! 4! 2! 0!

c5!

WT! bri1-116! bin2-1! a1a3!

Col! C5! UBC!

Hypocotyl (mm)!

Col b"#! B"# !

Fold change !

a

a!

c!

b! ATG!

pp2ab"$!

B’!

ATG!

pp2ab"#!

B’"

30 cycles! 60 cycles!

Fig. S6.

b!

a! 1!

214!

252!

-AH!

336!

△PEST3!

+AH! BD-B"#!

c!

BZR1-YFP-1! !P3-YFP-11! BZR1-YFP-5! !P3-YFP-15! BL

-

+

-

+

-

+

-

+!

pBZR1! BZR1!

!

p△P3-YFP !P3-YFP

!

!"#$%&

d! %P3-YFP-12

%P3-YFP-11

%P3-YFP-15

%P3-YFP-16 !

P△P3-YFP! Stain!

Fig. S7

PP2AB"#-GFP!

PP2AB"$-GFP!

PP2AB"%-GFP!

Fig. S8.