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SUPPLEMENTAL INFORMATION FOR:
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Mutational and gene expression analysis of mtrDEF, omcA, and mtrCAB during arsenate and iron reduction in Shewanella sp. ANA-3.
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Carolina Reyes, Julie N. Murphy, and Chad W. Saltikov*
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Depart. Microbiology and Environmental Toxicology
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*Corresponding author:
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University of California, Santa Cruz
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Dept. Microbiology and Environmental Toxicology
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1156 High Street Santa Cruz, CA 95064
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(tel.) 831-459-5520
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(fax) 831-459-3524
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(email)
[email protected]
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Running Title: Effects of arsenate on iron reduction in Shewanella
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Fig. S1. Growth of , wildtype; , ARM1; , FARM; , FERM and , No cell (A)
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aerobically or (B) anaerobically on nitrate, (C) fumarate, (D) TMAO and (E) arsenate.
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The time course for growth was inferred from the optical density at 600 nm. The data
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points and error bars represent the means and standard deviations of triplicate cultures,
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respectively.
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Fig. S2. Heme staining total membrane preparations of wild-type Shewanella sp. ANA-3
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(lanes 1, 3, 5, 7) and FERM (∆mtrDEF, ∆omcA, ∆mtrCAB) (lanes 2, 4, 6, 8) strain grown
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on various terminal electron acceptors: fumarate (lane 1-2), TME (lane 3-4), TMAO (lane
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5-6), As(V) (lane 7-8). L indicates 20 µl of Precision Plus Protein Kaleidoscope ™ (Bio
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Rad) prestained standard. The lanes 1-8 were loaded with 60 µg of total protein onto a
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10% SDS polyacrylamide gel.
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