Supplementary Information for High resolution mCT of a mouse embryo using a compact laserdriven x-ray betatron source Jason M. Cole, Daniel R. Symes, Nelson C. Lopes, Jonathan C. Wood, Kristjan Poder, Saleh Alatabi, Stan Botchway, Peta S. Foster, Sarah Gratton, Sara Johnson, Christos Kamperidis, Olena Kononenko, Michael De Lazzari, Charlotte A. J. Palmer, Dean Rusby, Jeremy Sanderson, Michael Sandholzer, Gianluca Sarri, Zsombor Szoke-Kovacs, Lydia Teboul, James M. Thompson, Jonathan R. Warwick, Henrik Westerberg, Mark A. Hill, Dominic P. Norris, Stuart P. D. Mangles, Zulfikar Najmudin Daniel Symes Email:
[email protected] This PDF file includes: Figs. S1 to S3 Captions for movies S1 to S2 Other supplementary materials for this manuscript include the following: Movies S1 to S2 1 of 5
www.pnas.org/cgi/doi/10.1073/pnas.1802314115
Fig. S1. Still image taken from Movie S1.
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Fig. S2. Still image taken from Movie S2.
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Fig. S3. Single x-ray projections and tomographic sagittal slices as shown in Fig. 3, but without the application of the sharpening function described in the Methods.
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Movie S1. Volume rendering of the 3D tomographic reconstruction from the laser-betatron scan, rendered with the Drishti visualisation software. For computational ease this rendering was performed using a voxel array downsized by a factor of 5 in each dimension. Each of the voxels in the rendered array is the median of the nearest 125 voxels in the original array. Movie S2. Tomographic reconstruction from the laser-betatron scan similar to that shown in Movie S1 but with the volume rendering options adjusted to make lower density regions appear more transparent, enhancing the appearance of internal features.
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