PP2A. PP2A. RAS. NF1. RAS. MAP3K1. MAPK1. MNK. TSC1/2. TSC1/2. RHEB. FMRP. FMRP. mGLUR. MET. NR. mGLUR. PTEN. Ca2+. New protein synthesis.
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doi:10.1038/nature11737
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Supplementary Figure 9.
460 268 238 -
C trl . FX S p C ati trl en . t1 FX S p C ati trl en . t2 FX S p C ati trl en . t3 FX S pa tie nt 4
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171 117 FMRP
71 55 -
PABPC1
Supplementary Figure 9. Expression of FMR1 in post-mortem brain lysates prepared from FXSaffected and normal individuals. An immunoblot for FMRP protein in the anterior cingulate (BA24, pre-frontal cortex) region is shown. Similar FMR1 protein levels were observed in hippocampus and cerebellar regions. Loading was normalized using PABPC1.
Supplementary Figure 10.
mGLUR
mGLUR
NR
MET
Homer
Ca 2+
RAS NF1
RAS MAP3K1
MAPK1
PP2A
PIKE PI3K
PDK1/2
PTEN
New protein synthesis
AKT TSC1/2 TSC1/2
MTOR
RAPTOR
RHEB
PP2A S6K S6
MNK
Cap
EIF4E 4E-BP2
EIF4E
4E-BP2
40S
EIF4G1
FMRP
FMRP
aaaa
4A
Supplementary Figure 10. Signaling pathways and targets of FMRP. The role of FMRP may lie within its ability to finely regulate the translation and/or transport of groups of functionally-related genes, which themselves can modulate the activity of FMRP at the protein level. MTOR, MGLUR, and RAS signal transduction pathways are shown. Proteins encoded by ASD-related genes are colored in dark (transcript detected in PAR-CLIP) and light (transcript not detected in PAR-CLIP due to lack of expression) green. Yellow indicates signaling pathway components also identified by PAR-CLIP.
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SUPPLEMENTARY TABLES Supplementary Tables were generated in Microsoft Excel 2011 (Mac) in *.xls format. The combined set are available as .zip compressed files.
