a Degrees of freedom of the numerator b Degrees of freedom of the denominator. Table 2. Linear models (ANOVA) analyzing, for each of the two replicates at ...
Supplementary material Table 1. Linear models (ANOVA) analyzing, for each immunological parameter studied, the difference between the two replicates at 8.8°C. Parameter
num d.f.a, den d.f.b
F
P
Bacteria in gammarids
1, 66
0.008
0.93
Hemocyte concentration
1, 86
6.98
0.01
Active PO activity
1, 86
1.72
0.19
Total PO activity
1.85
4.5
0.04
a
Degrees of freedom of the numerator
b
Degrees of freedom of the denominator
Table 2. Linear models (ANOVA) analyzing, for each of the two replicates at 8.8°C, the number of bacteria remaining in 1 µl of gammarid hemolymph as a function of gammarids size, temperature and its quadratic effect (temperature²). Models
Source of variation
num d.f.a, den d.f.b
F
Replicate 1
Global Model
3, 131
3.96
0.010
Size
1, 131
5.32
0.023
Temperature
1, 131
0.03
0.87
Temperature2
1, 131
6.54
0.012
Global Model
3, 136
3.63
0.015
Size
1, 136
4.42
0.037
Temperature
1, 136
0.03
0.87
Temperature2
1, 136
6.32
0.013
Replicate 2
a
Degrees of freedom of the numerator
b
Degrees of freedom of the denominator
P
Table 3. Multivariate (MANOVA, Pillai’s trace) analyses of variance, for each of the two replicates at 8.8°C, showing the three immunological parameters (hemocyte concentration, active and total phenoloxidase enzymatic activity) as a function of gammarids size, temperature and its quadratic effect (temperature²). Models
Source of variation
num d.f.a, den d.f.b
F
Replicate 1
Global Model
9, 495
5.22
< 0.0001
Size
3, 163
9.20
< 0.0001
Temperature
3, 163
1.87
0.14
Temperature2
3, 163
5.71
0.001
Global Model
9, 513
4.65
< 0.0001
Size
3, 169
10.43
< 0.0001
Temperature
3, 169
0.18
0.91
Temperature2
3, 169
4.01
0.009
Replicate 2
a
Degrees of freedom of the numerator
b
Degrees of freedom of the denominator
P
Fig. 1. Effect of temperature on the number of bacteria: average number of bacteria (colony-forming units) remaining in 1 µl of gammarid hemolymph, at each temperature tested. The transformed data are presented here. Points represent mean values, error bars stand for 95% confidence intervals, and sample sizes of gammarids are indicated.
Fig. 2. Effect of acclimatization temperature on the different immunological parameters. (A) Coordinates of the first canonical axis generated by the MANOVA (combination of the three immunological parameters), (B) hemocyte concentration, (C) active phenoloxidase activity and (D) total phenoloxidase activity. The transformed data are presented here. Points represent mean values, error bars stand for 95% confidence intervals and sample sizes are indicated.
