Supplementary Materials - MDPI

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Dilution Used. Manufacturer anti-α-Tubulin. mAb mouse. T5168. 1:5000. Sigma-Aldrich anti-β-actin. mAb mouse. CP01. 1:10000. Calbiochem anti-caspase-3.

1

Supplementary Materials Table 1. Description of aantibodies Figure 1. Effect of mycalamide A on cell viability of HeLa cells, detected by the cytotoxicity assay (MTS test) Figure 2A. 1H NMR spectrum of mycalamide A in CD3OD (0–6.0 ppm) Figure 2B. 1H NMR spectrum of mycalamide A in CD3OD (crop 0–1.8 ppm) Figure 2C. 1H NMR spectrum of mycalamide A in CD3OD (crop 1.8–2.7 ppm) Figure 2D. 1H NMR spectrum of mycalamide A in CD3OD (crop 3.0–4.0 ppm) Figure 2E. 1H NMR spectrum of mycalamide A in CD3OD (crop 4.0–4.9 ppm) Figure 2F. 1H NMR spectrum of mycalamide A in CD3OD (crop 5.0–6.0 ppm) Figure 3. 13C NMR spectrum of mycalamide A in in DMSO-d6

2 Table 1. Description of antibodies. Antibodies

Clonality

Source

Cat.-No.

Dilution Used

Manufacturer

anti-α-Tubulin anti-β-actin anti-caspase-3 anti-ERK anti-JNK anti-p38 anti-phospho-ERK anti-phospho-JNK anti-phospho-p38 anti-mouse IgG-HRP anti-rabbit IgG-HRP

mAb mAb mAb mAb mAb mAb mAb mAb mAb

mouse mouse rabbit mouse rabbit rabbit rabbit rabbit rabbit sheep goat

T5168 CP01 #9665 #9107 #9258 #9212 #4377 #4668 #4511 NXA931 #7074

1:5000 1:10000 1:1000 1:2000 1:1000 1:1000 1:1000 1:1000 1:1000 1:10000 1:5000

Sigma-Aldrich Calbiochem Cell Signaling Cell Signaling Cell Signaling Cell Signaling Cell Signaling Cell Signaling Cell Signaling GE Healthcare Cell Signaling

100

** *** ***

50

*** ***

Mycalamide A, [nM]

50

25

1. 56 3 3. 12 5 6. 25 12 .5

0

*** 10 0

*** 0

Cell viability, % of control

Figure 1. Cell viability of HeLa cells under treatment with different concentrations of mycalamide A. Cytotoxicity was determined using the MTS method. Statistically significant differences (determined by t-test) between treated and control cells are indicated as follows: ** p