Supplementary Table 4

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Supplementary Table 4

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PSMA4. 19. 42. RAN GTPase activating protein 1. P62826. RANGAP1. 40. 40. Other. Supplementary Table 4. KEGG pathway classification of identified proteins.

Spot number

Description

UniProt KB Gene name entry

% of spot volume variation N72L vs K72L

N72H vs K72H

Metabolism 7

Transketolase

P40142

TKT

20

P09411

PGK1

50

71

21

Phosphoglycerate kinase 1 Fructose-bisphosphate aldolase A

P05064

ALDOA

60

80

27

Glyceraldehyde-3-phosphate dehydrogenase

P16858

GAPDH

22

44

28

Lactate dehydrogenase A triosephosphate isomerase 1

P06151

LDHA

58

70

P17751

TPI1

64

71

41

- 27

Amminoacid/lipid metabolism 22

Acetyl-Coenzyme A acetyltransferase 2

Q8CAY6

ACAT2

62

62

23

Acetyl-Coenzyme A acetyltransferase 2

Q8CAY6

ACAT2

32

121

37

Methylthioadenosine phosphorylase

Q9CQ65

MTAP

68

52

- 30

- 39

Protein synthesis and folding 5

Glycyl-tRNA synthetase

Q9CZD3

GARS

8

P14211

CALR

9

Calreticulin T-complex protein 1 subunit alpha

P11983

TCP1

10

Chaperonin containing Tcp1, subunit 5 (epsilon)

P80316

CCT5

40

39

11

Peptidyl-prolyl cis-trans isomerase FKBP4 (FK506-binding protein 4)

P30416

FKBP4

40

49

12

Protein disulfide isomerase associated 4

P09103

PDIA4

13

Chaperonin containing Tcp1, subunit 2 (beta)

P80314

CCT2

31

17

Eukaryotic translation initiation factor 4A1 Peptidyl-prolyl cis-trans isomerase A

Q4FZL1

EIF4A1

38

P17742

PPIA

23

45

37 37

- 36

Stress response 2

Heat shock protein 90, beta (Grp94), member 1

P08113

HSP90B1

6

Stress-70 protein, mitochondrial

P38647

HSPA9

21

19

Serine (or cysteine) peptidase inhibitor, clade B, member 6a

Q60854

SERPINB6A

- 26

- 32

30

Esterase D/formylglutathione hydrolase

Q9R0P3

ESD

87

49

33

Proteasome (prosome, macropain) subunit, alpha type 1

Q9R1P4

PSMA1

27 79

- 10

34

Glutathione S-transferase omega 1

O09131

GSTO1

39

Peroxiredoxin 4

O08807

PRDX4

57

40

O08709

PRDX6

37

43

Peroxiredoxin 6 Superoxide dismutase [Mn], mitochondrial

P09671

SOD2

51

30

44

Peroxiredoxin 1

P35700

PRDX1

45

35

33

Structural proteins 1

Vinculin

Q64727

VCL

- 62

- 50

3

Gelsolin

P13020

GSN

- 41

- 54 - 53

4

Gelsolin

P13020

GSN

- 48

15

Vimentin

P20152

VIM

69

39

16

Vimentin

P20152

VIM

106

124

18

Vimentin

P20152

VIM

125

36

Lectin, galactose binding, soluble 3

Q8C253

LGALS3

14

RuvB-like protein 2

Q9WTM5

RUVBL2

57

24

P62137

PPP1CA

27

25

Protein phosphatase 1, catalytic subunit, alpha isoform Annexin A1 (Lipocortin I)

P10107

ANXA1

- 23

26

Annexin A3

O35639

ANXA3

- 59

29

Annexin A5

P48036

ANXA5

65

31

Voltage-dependent anion channel 1

Q60932

VDAC1

- 25

32

Guanine nucleotide binding protein (G protein), beta polypeptide 2 like 1 (Gnb2l1), mRNA

P68040

GNB2L1

16

35

Chloride intracellular channel 1 Proteasome subunit alpha type-4

Q9Z1Q5

CLIC1

46

Q9R1P0 P62826

PSMA4 RANGAP1

19 40

183 - 31

Other

38 42

RAN GTPase activating protein 1

40

- 52 84

Supplementary Table 4 KEGG pathway classification of identified proteins. The two columns on the right indicate the percentage of protein spot variation as measured on 2-DIGE gels.