Electronic Supplementary Material (ESI) for New Journal of Chemistry. This journal is © The Royal Society of Chemistry and the Centre National de la Recherche Scientifique 2015
Supporting Information A highly selective fluorescent chemosensor for Hg2+ based on a hydrazones derivative including phthalazin and their application in human cervical cancer HeLa cells Dipesh Mahajana, Nilesh Khairnara, Banashree Bondhopadhyayc, Suban K.Sahoob, Anupam Basuc, Jasminder Singhd, Narinder Singh*d, Ratnamala Bendre*a, Anil Kuwar*a aSchool
of Chemical Sciences, North Maharashtra University, Jalgaon- 425001 (MS) India.
Email:
[email protected],
[email protected] bDepartment cMolecular
of Chemistry, SV National Institute Technology, Surat (Gujarat) India.
Biology and Human Genetics Laboratory Department of Zoology The University
of Burdwan, Burdwan, West Bengal, India. dDepartment
of Chemistry, Indian Institute Technology, Ropar-140001 (Punjab) India.
Email:
[email protected]
WATERS, Q-TOF MICROMASS (LC-MS)
SAIF/CIL,PANJAB UNIVERSITY,CHANDIGARH
R S BENDRE D-2 9 (0.095) Cm (9:39)
TOF MS ES+ 2.08e4
336.1 20763
%
100
337.2 4046
0
100
150
200
250
300
350
400
450
500
550
600
650
700
750
Figure S1: LC-MS Spectra of receptor 1
800
850
900
950
1000
m/z
Figure S2: 1H NMR Spectrum of receptor 1
Figure S3: 13C NMR Spectrum of receptor 1
3.00E+06 Intensity (cps)
2.50E+06 2.00E+06 1.50E+06 1.00E+06 5.00E+05
R
ec
ep C tor o( N II) i( C II) u( M II) g( Zn II) ( H II) g( I Zr I) ( C I) s( C I) d( C II) a( Ba II) ( Fe II) ( Fe II) (I Pb II) M (II) n( II K ) A (I) l(I Sr II) ( N II) a( II )
0.00E+00
Cation
Figure S4:Bar diagram showing effect of 1 equivalents of different metal ions on the fluorescent intensity of 1 (10 μM) in DMSO/H2O (80:20, v/v) solution.
3.50E-07
1/ΔF
3.00E-07 2.50E-07 2.00E-07 1.50E-07 1.00E-07 5.00E-08 0.00E+00 0
2000
4000
6000
8000
1/[G]
10000 12000 14000 16000
Figure S5. Benesi-Hildebrand plot for receptor 1, 1/ΔF vs 1/[Hg2+].
Figure S6: Plot of relative fluorescence intensity of sensor1 and Hg2+ at different concentrations (5, 10, 15 and 20 M) as a function of time (seconds).
Blank Maxima SD of 460 9.66E+04 9.79E+04 1.02E+05 Blank 2.60E+03
Slope from Graph
LOD LOQ 2.61E7.91E3.29E+00 09 09
26.1 nM 79.1 nM Table S1: Calculation of Detection limit using IUPAC 3method and LOQ measurement using 10method
