Electronic Supplementary Material (ESI) for RSC Advances. This journal is © The Royal Society of Chemistry 2014
Supporting Information Photophysics of Lumichrome in Anionic and Cationic Micellar Media Banibrata Maity, Aninda Chatterjee and Debabrata Seth* Department of Chemistry, Indian Institute of Technology Patna Patna 800013, Bihar, India E-mail :
[email protected]; Fax : 91-612-2277383
1.0 (b)
450 nm 570 nm
Fluorescence intensity (a.u.)
Fluorescence intensity (a.u.)
1.0 (a) 0.8 0.6 0.4 0.2 0.0 300
350
400
Wavelength (nm)
450
0.8 0.6 0.4 0.2 0.0 300
500
450 nm 570 nm
350
400
Wavelength (nm)
Fluorescence intensity (a.u.)
Fluorescence intensity (a.u.)
1.0 (d) 1.0 (c)
450 nm 570 nm
0.8 0.6 0.4 0.2 0.0 300
350
400
Wavelength (nm)
450
500
450
500
450 nm 570 nm
0.8 0.6 0.4 0.2 0.0 300
350
400
Wavelength (nm)
450
500
Fig. S1. The fluorescence excitation spectra of LCM in (a) pure water, (b) SDS, (c) DTAB and (d) MTAB micelles. The observation wavelengths were at 450 nm (black) and 570 nm (red).
1
(a)
23 nm
Normalised fluorescence intensity (a.u.)
Normalised fluorescence intensity (a.u.)
1.0
375 nm 420 nm 445 nm
0.8 0.6 0.4 0.2 0.0 450
500
550
600
650
Normalised fluorescence intensity (a.u.)
Wavelength (nm)
1.0
(c)
1.0
(b)
63nm
375 nm 420 nm 430 nm 445 nm
0.8 0.6 0.4 0.2 0.0 450
500
550
600
650
Wavelength (nm)
52 nm
375 nm 420 nm 430 nm 445 nm
0.8 0.6 0.4 0.2 0.0
450
500
550
600
650
Wavelength (nm)
Fig. S2. The excitation wavelength dependent emission shift of LCM in the presence of (a) SDS, (b) DTAB and (c) MTAB micelles (exi = 375 to 445 nm).
(b)
(a)
150000
140000
130000
Intensity
Intensity
140000
120000
130000
120000
110000 100000
110000
0.000
0.001
0.002
0.003
0.004
[SOS-Micelle]-1 (M-1)
0.005
0.006
0.0000
0.0005
0.0010
0.0015
0.0020
[SDS-Micelle]-1 (M-1)
Fig. S3. The plot of fluorescence intensity against [Micelle] in (a) SOS and (b) SDS micelles.
2
0.0025
5000
(a)
3000 2000 1000
neat water 16 mM DTAB 30 mM DTAB 60 mM DTAB 165 mM DTAB 310 mM DTAB IRF
3000 2000 1000
0
1
2
Time (ns)
5000
3
4
0
5
(c)
0
1
2
Time (ns)
3
4
neat water 5 mM MTAB 10 mM MTAB 25 mM MTAB 45 mM MTAB 90 mM MTAB
4000
Intensity
0
(b)
4000
Intensity
4000
Intensity
5000
neat water 20 mM SDS 160 mM SDS IRF
3000 2000 1000 0
0
1
2
3
Time (ns)
4
5
Fig. S4. The time resolved fluorescence emission decays of LCM with gradual addition of (a) SDS, (b) DTAB and (c) MTAB micelles, beyond their respective CMC values (exi = 375 nm).
3
5
5000
(a)
580 nm 454 nm 425 nm IRF
Intensity
2000 1000 0
580 nm 525 nm 455 nm 425 nm IRF
4000
3000
3000 2000 1000
0
1
2
3
Time (ns)
5000
0
4
0
Intensity
1
2
Time (ns)
(c) 580 nm 525 nm 458 nm 425 nm IRF
4000 3000 2000 1000 0
0
1
Time (ns)
2
3
Fig. S5. The emission wavelength dependent fluorescence emission decays of LCM in (a) SDS, (b) DTAB and (c) MTAB micelles.
1.0
(a)
Normalised Intensity (a.u.)
0.05 0.04
Absorbance
Intensity
4000
(b)
5000
0.03 0.02 0.01 0.00
350
400
450
Wavelength (nm)
500
550
0.8 0.6 0.4 0.2 0.0
400
450
500
Wavelength (nm)
550
600
Fig. S6. (a) The absorption and (b) emission spectra of LCM in isooctane.
4
1.0
pure water 20 cmc BHDC
Fluorescence intensity (a.u.)
Fluorescence intensity (a.u.)
1.0 (a) 0.8 0.6 0.4 0.2 0.0 400
450
500
550
600
(b)
0.8 0.6 0.4 0.2 0.0 400
650
pure water 20 mM KBr 1 M KBr
450
Fluorescence intensity (a.u.)
0.8 0.6 0.4 0.2 0.0 400
450
500
550
1.0 (d)
pure water 20 mM TMAB 1 M TMAB
550
600
600
650
pure water 20 mM KCl 1 M KCl
0.8 0.6 0.4 0.2 0.0 400
650
Wavelength (nm)
1.0 (e)
Fluorescence intensity (a.u.)
Fluorescence intensity (a.u.)
1.0 (c)
500
Wavelength (nm)
Wavelength (nm)
450
500
550
Wavelength (nm)
600
pure water 20 mM TMAC 1 M TMAC
0.8 0.6 0.4 0.2 0.0 400
450
500
550
Wavelength (nm)
600
650
Fig. S7. The emission spectral profile of LCM (exi = 375 nm) in (a) BHDC micelles and in presence of (b) KBr, (c) TMAB, (d) KCl and (e) TMAC salts.
5
650
5000 (a) 4000 3000 2000
0
1
2
3
4
Time (ns)
5000 (c)
2000
0
5
1
2
3
Time (ns)
Counts
2000
4
5
pure water 20 mM KCl 1 M KCl IRF
4000
3000
3000 2000 1000
1000
0
1
2
3
Time (ns)
4
0
5
5000 (e)
0
1
2
3
4
Time (ns)
pure water 20 mM TMAC 1 mM TMAC IRF
4000
Counts
0
0
5000 (d)
pure water 20 mM TMAB 1 M TMAB IRF
4000
Counts
3000
1000
1000 0
pure water 20 mM KBr 1 M KBr IRF
4000
Counts
Counts
5000 (b)
pure water 20 cmc BHDC IRF
3000 2000 1000 0
0
1
2
3
Time (ns)
4
5
Fig. S8. The time resolved fluorescence emission decays of LCM in (a) BHDC micelles and in presence of (b) KBr, (c) TMAB, (d) KCl and (e) TMAC salts.
6
5
Table S1 The fluorescence lifetime components of LCM in presence of BHDC micelles and in different salts (exi = 375 nm, emi = 470 nm).
1 (ns)
Medium
a1
2 (ns)
a2
LCM in pure water LCM in BHDC micellesa,b (20 CMC) LCM in 20 mM KBr
0.200
LCM in 1M KBr
0.090
LCM in 20 mM TMAB LCM in 1M TMAB
0.110
0.340
0.990
0.990
LCM in 20 mM KCl LCM in 1M KCl LCM in 20 mM TMAC LCM in 1M TMAC
aCMC bZ.
0.220
0.260
0.985
0.984
0.670
0.350
3 (ns)
a3 1.000
f> (ns)
2.700
1.084
2.630
0.310
1.110
1.030
1.900
1.000
1.900
1.050
0.470
0.010
0.090
1.040
2.050
1.000
2.050
1.100
0.710
0.001
0.116
1.286
2.13
1.00
2.130
0.99
1.47
0.015
0.240
1.097
2.06
1.00
2.06
1.00
1.47
0.016
0.280
1.080
of BHDC = 0.49 mMb
Grenoble and S. Baldelli, J. Phys. Chem. B 2013, 117, 259.
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