Sigma-Aldrich Sigma-Aldrich New England Biolabs New England Biolabs
NA NA M2200L B7002S
3840 40 388 18
576000 6000 300 384
0.01 0.01 1.29 0.05
PCR Enrichment Phusion Mastermix Primer oligos
New England Biolabs Sigma-Aldrich
M0531S NA
170 47
9600 28800000
0.02 0.000002
DNA fragmentation
TOTAL for Reagents Clean-ups AmpPure XP Magnetic Beads
2.96
Beckman-Coulter
A63881
1126
508
TOTAL for Library prep Validation Qubit DNA quantification reagents Bioanlayzer reagents
5.18
Life Technologies Agilent Technologies
Q32851 5067-1505
79 349
9600 28800
TOTAL for Preparation/Validation Pre-Library Prep Quantification Qubit DNA quantification reagents TOTAL for all
2.22
0.01 0.01 5.20
Life Technologies
Q32851
79
100
0.79 5.99
Illumina Tru-Seq Nano Manufacturer
Part number Price per package
Covaris
520045
Illumina
FC-121-4003
Samples per Price per package sample
DNA Fragmentation 125
25
5
2880
96
30
Library Prep TruSeq Nano TOTAL for Library prep
35
Validation Qubit DNA quantification reagents Bioanlayzer reagents
Life Technologies Agilent Technologies
Q32851 5067-1505
79 349
9600 28800
TOTAL for Preparation/Validation Pre-Library prep Quantification Qubit DNA quantification reagents TOTAL for all
0.01 0.01 35.02
Life Technologies
Q32851
79
100
0.79 35.81
Prices are based on the list price for the US market (in US dollars). The reagents needed for library quantification for normalization purposes, plastic consumables such as 96-well PCR plates, and general laboratory reagents are not included, as they not included in the Illumina TruSeq kit.
