TOC - Journal of Bacteriology

JOURNAL OF BACTERIOLOGY Volume 171

September 1989

No. 9

GENERAL MICROBIOLOGY Interference Reflection Microscopic Study of Sites of Association between Gliding Bacteria and Glass Substrata. Stephen L. Godwin, Madilyn Fletcher, and Robert P. Burchard .................................................. Cell-Density-Dependent Lysis and Sporulation of Myxococcus xanthus in Agarose Microbeads. Amy Rosenbluh, Raphael Nir, Elhanan Sahar, and Eugene Rosenberg. Evidence for Two Different Types of Insecticidal P2 Toxins with Dual Specificity in Bacillus thuringiensis Subspecies. Colin N. Nicholls, Wasim Ahmad, and David J. Ellar ................................................... High-Frequency Variation in Mycoplasma pulmonis Colony Size. Kevin Dybvig, Jerry W. Simecka, Harold L. Watson, and Gail H. Cassell ...............

STRUCTURE AND FUNCTION Cell Length, Nucleoid Separation, and Cell Division of Rod-Shaped and Spherical Cells of Escherichia coli. William D. Donachie and Kenneth J. Begg ...... Fimbriation Genes of Salmonella enteritidis. Karl-Heinz Muller, Trevor J. Trust, and William W. Kay ........................................... Cell-Cycle-Dependent Polar Morphogenesis in Caulobacter crescentus: Roles of Phospholipid, DNA, and Protein Syntheses. Edward A. O'Neill and Robert A. Bender.......................................................... Variation in Buoyant Density of Whole Cells and Isolated Cell Walls of Streptococcus faecium (ATCC 9790). David Glaser, Michael Haines, James Bylund, and Michael Higgins......................................... Both CheA and CheW Are Required for Reconstitution of Chemotactic Signaling in Escherichia coli. M. Patricia Conley, Alan J. Wolfe, David F. Blair, and Howard C. Berg ................................................. Examination of Enterotoxigenic Escherichia coli H10407 (Colonization Factor Antigen I+) by Scanning Electron Microscopy with Conductive Staining. Richard Sherburne and Glen D. Armstrong ............................. PLANT MICROBIOLOGY Developmental Regulation of a Rhizobium Cell Surface Antigen during Growth of Pea Root Nodules. Kathryn A. VandenBosch, Nicholas J. Brewin, and Elmar L. Kannenberg ................................................ Expression of a Cell Surface Antigen from Rhizobium leguminosarum 3841 Is Regulated by Oxygen and pH. Elmar L. Kannenberg and Nicholas J. Brewin Genetic Derepression of a Developmentally Regulated Lipopolysaccharide Antigen from Rhizobium leguminosarum 3841. Elizabeth A. Wood, Geoffrey W. Butcher, Nicholas J. Brewin, and Elmar L. Kannenberg ................. Immunogold Localization of the NodC and NodA Proteins of Rhizobium meliloti. Deane Johnson, L. Evans Roth, and Gary Stacey......................

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Subcellular Localization of the nodD Gene Product in Rhizobium leguminosarum. Helmi R. M. Schlaman, Herman P. Spaink, Robert J. H. Okker, and Ben J. J. Lugtenberg ..................................................... Rhizobium leguminosarum Exopolysaccharide Mutants: Biochemical and Genetic Analyses and Symbiotic Behavior on Three Hosts. Ronald Diebold and K. Dale Noel........................................................... The Predicted Protein Product of a Pathogenicity Locus from Pseudomonas syringae pv. phaseolicola Is Homologous to a Highly Conserved Domain of Several Procaryotic Regulatory Proteins. C. Grimm and N. J. Panopoulos ........

GENETICS AND MOLECULAR BIOLOGY Characterization of the Major DNA Repair Methyltransferase Activity in Unadapted Escherichia coli and Identification of a Similar Activity in Salmonella typhimurium. G. William Rebeck, C. Mark Smith, Dale L. Goad, and Leona Samson.................................................. New Method for Generating Deletions and Gene Replacements in Escherichia coli. Carol M. Hamilton, Marti Aldea, Brian K. Washburn, Paul Babitzke, and Sidney R. Kushner..................................... The yopM Gene of Yersinia pestis Encodes a Released Protein Having Homology with the Human Platelet Surface Protein GPIba. K. Y. Leung and S. C. Straley. Induction of Anaerobic Gene Expression in Rhodobacter capsulatus Is Not Accompanied by a Local Change in Chromosomal Supercoiling as Measured by a Novel Assay. David N. Cook, Gregory A. Armstrong, and John E. Hearst Conservation of Genomic Sequences among Isolates of Mycobacterium leprae. Josephine E. Clark-Curtiss and Gerald P. Walsh ........................ Saturation Mutagenesis of an Escherichia coli rRNA Promoter and Initial Characterization of Promoter Variants. Tamas Gaal, John Barkei, Ramona R. Dickson, Herman A. deBoer, Pieter L. deHaseth, Hossain Alavi, and Richard L. Gourse.................................................. Identification of Promoter Mutants Defective in Growth-Rate-Dependent Regulation of rRNA Transcription in Escherichia coli. Ramona R. Dickson, Tamas Gaal, Herman A. deBoer, Pieter L. deHaseth, and Richard L. Gourse ............................................................. Gene Cluster Containing the Genes for Tyrocidine Synthetases 1 and 2 from Bacillus brevis: Evidence for an Operon. Gerhard Mittenhuber, Renate Weckermann, and Mohamed A. Marahiel.............................. Transformation of a Conditionally Peptidoglycan-Deficient Mutant of Staphylococcus aureus with Plasmid DNA. Daniel T. Nieuwlandt and P. A. Pattee .... Transcription Initiation at Multiple Promoters of the pfl Gene by EW70-Dependent Transcription In Vitro and Heterologous Expression in Pseudomonas putida In Vivo. Gary Sawers, A. F. Volker Wagner, and August Bock.......... Translesion Synthesis Is the Main Component of SOS Repair in Bacteriophage Lambda DNA. Martine Defais, Claire Lesca, Bernard Monsarrat, and Philip Hanawalt.................................................... Transformation of the Archaebacterium Halobacterium volcanii with Genomic DNA. Steven W. Cline, Leonard C. Schalkwyk, and W. Ford Doolittle Temperature-Sensitive Lethal Mutant of Era, a G Protein in Escherichia coli. Toshifumi Inada, Koichi Kawakami, Su-Min Chen, Howard E. Takiff, Donald L. Court, and Yoshikazu Nakamura ............................

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Evidence for Unlinked rrn Operons in the Planctomycete Pirellula marina. Werner Liesack and Erko Stackebrandt ................................ Molecular Characterization of Two cya Mutations, cya-854 and cyaRI. P. Glaser, A. Roy, and A. Danchin...................................... Evidence for Unique DNA Repair Activity Encoded by a Cloned Serratia marcescens Gene: Suppression of Escherichia coli Mutations That Reduce Repair of Alkylated DNA. Keith E. Murphy, Sami N. Guzder, and H. Douglas Braymer ............................................................ Levels of DNA Topoisomerases, Single-Stranded-DNA-Binding Protein, and DNA Polymerase I in rho+ and rho-15 Strains of Escherichia coli. Gail Ferstandig Arnold, Teresa A. Phillips, and Irwin Tessman................ In Vitro Construction of gshB::kan in Escherichia coli and Use of gshB::kan in Mapping the gshB Locus. Thomas Daws, Chang-Jin Lim, and James A. Fuchs .............................................................. Cloning and Sequencing of an Escherichia coli Gene, nlp, Highly Homologous to the ner Genes of Bacteriophages Mu and D108. Yong-Lark Choi, Tohru Nishida, Makoto Kawamukai, Ryutaro Utsumi, Hiroshi Sakai, and Tohru Komano ............................................................ Influence of spo Mutations on SE Synthesis in Bacillus subtilis. Robert M. Jonas and William G. Haldenwang .......................................... Characterization of the Pleiotropic Phenotypes of Rifampin-Resistant rpoB Mutants of Escherichia coli. Ding Jun Jin and Carol A. Gross ...............

PLASMIDS AND TRANSPOSONS Region of the Streptococcal Plasmid pMV158 Required for Conjugative Mobilization. Scott D. Priebe and Sanford A. Lacks ........................... Participation of the Lytic Replicon in Bacteriophage P1 Plasmid Maintenance. Michael B. Yarmolinsky, Egon B. Hansen, Samina Jafri, and Dhruba K.

Chattoraj.......................................................... Genetic and Molecular Analyses of the Gene Encoding Staphylococcal Enterotoxin D. Kenneth W. Bayles and John J. landolo............................ Method for Selection of Transposable DNA and Characterization of a New Insertion Sequence, IS493, from Streptomyces lividans. Patricia J. Solenberg and Stanley G. Burgett.................................................. Staphylococcus aureus Chromosomal Mutation plaCI Amplifies Plasmid pT181 by Depressing Synthesis of Its Negative-Effector Countertranscripts. Serban Iordanescu .......................................................... Role of Countertranscript RNA in the Copy Number Control System of an IncB Miniplasmid. J. Praszkier, P. Bird, S. Nikoletti, and J. Pittard........... Cloning and Expression of Plasmid Genes Encoding Resistances to Chromate and Cobalt in Alcaligenes eutrophus. Anke Nies, Dietrich H. Nies, and Simon Silver............................................................... Cloning of pMOL28-Encoded Nickel Resistance Genes and Expression of the Genes in Akcaligenes eutrophus and Pseudomonas spp. Roman A. Siddiqui, Karin Benthin, and Hans G. Schlegel .................................. Degradation of Phenol and m-Toluate in Pseudomonas sp. Strain EST1001 and Its Pseudomonas putida Transconjugants Is Determined by a Multiplasmid System. Maia A. Kivisaar, Jaan K. Habicht, and Ain L. Heinaru ...........

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Insertion Elements and Deletion Formation in a Halophilic Archaebacterium. Felicitas Pfeifer and Ulrike Blaseio .................................... Orientation of IS50 Transposase Gene and IS50 Transposition. John C. Makris and William S. Reznikoff ............................................. EUCARYOTIC CELLS Temporal Expression of Transcription and Relative Copy Number of Plasmid pSFB-1 in Scytalidiumflavo-brunneum. Daniel R. Caprioglio and Leo W. Parks .

CELL SURFACES Genetic Studies on the Inability of ,1-Galactosidase To Be Translocated across the Escherichia coli Cytoplasmic Membrane. Catherine Lee, Ping Li, Hiroshi Inouye, Edith R. Brickman, and Jon Beckwith......................... Mutations That Improve Export of Maltose-Binding Protein in SecB- Cells of Escherichia coli. David N. Collier and Philip J. Bassford, Jr............. Nucleotide Sequence of the Transcriptional Control Region of the Osmotically Regulated proU Operon of Salmonella typhimurium and Identification of the 5' Endpoint of the proU mRNA. David G. Overdier, Eric R. Olson, Bruce D. Erickson, Martina M. Ederer, and Laszlo N. Csonka ........... Two Functional psbD Genes in the Cyanobacterium Synechococcus sp. Strain PCC 7942. Susan S. Golden, Dan-Sung C. Cho, and Mark S. Nalty...... Magnesium Transport in Salmonella typhimurium: Genetic Characterization and Cloning of Three Magnesium Transport Loci. S. Paul Hmiel, Marshall D. Snavely, Jane B. Florer, Michael E. Maguire, and Charles G. Miller ...... Magnesium Transport in Salmonella typhimurium: Expression of Cloned Genes for Three Distinct Mg2' Transport Systems. Marshall D. Snavely, Jane B. Florer, Charles G. Miller, and Michael E. Maguire ...................... Magnesium Transport in Salmonella typhimurium: 28Mg2+ Transport by the CorA, MgtA, and MgtB Systems. Marshall D. Snavely, Jane B. Florer, Charles G. Miller, and Michael E. Maguire ............................. Genes Downstream from pucB and pucA Are Essential for Formation of the B800-850 Complex of Rhodobacter capsulatus. Hans Volker Tichy, Beatrice Oberle, Harald Stiehle, Emile Schiltz, and Gerhart Drews................ Demonstration of Rare Protein in the Outer Membrane of Treponema pallidum subsp. pallidum by Freeze-Fracture Analysis. Eldon M. Walker, Guido A. Zampighi, David R. Blanco, James N. Miller, and Michael A. Lovett...... Topology and Acylation of Spiralin. H. Wroblewski, S. Nystrom, A. Blanchard, and A. Wieslander....................................... Import of Biopolymers into Escherichia coli: Nucleotide Sequences of the exbB and exbD Genes Are Homologous to Those of the tolQ and tolR Genes, Respectively. Katrin Eick-Helmerich and Volkmar Braun.................. Involvement of ExbB and TonB in Transport across the Outer Membrane of Escherichia coli: Phenotypic Complementation of exb Mutants by Overexpressed tonB and Physical Stabilization of TonB by ExbB. Eckhard Fischer, Karolin Gunter, and Volkmar Braun ........................... Binding-Protein-Dependent Alanine Transport in Rhodobacter sphaeroides Is Regulated by the Internal pH. Tjakko Abee, Fimme-Jan van der Wal, Klaas J. Hellingwerf, and Wil N. Konings.............................

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A New ,1-Lactam-Binding Protein Derived from Penicillin-Binding Protein 3 of Escherichia coli. R. Prats, M. Gomez, J. Pla, B. Blasco, and J. A. Ayala

PHYSIOLOGY AND METABOLISM Expression of Zymomonas mobilis adhB (Encoding Alcohol Dehydrogenase II) and adhB-lacZ Operon Fusions in Recombinant Z. mobilis. Kylie F. Mackenzie, Tyrrell Conway, H. C. Aldrich, and L. 0. Ingram............ Role of Myxococcus xanthus Cell Surface Antigen 1604 in Development. Bruce W. Jarvis and Martin Dworkin ........................................ Posttranslational Regulatory System for Nitrogenase Activity in Azospirillum spp. Haian Fu, Anton Hartmann, Robert G. Lowery, Wayne P. Fitzmaurice, Gary P. Roberts, and Robert H. Burris..................... An Osmoregulated Dipeptide in Stressed Rhizobium meliloti. Linda Tombras Smith and Gary M. Smith............................................. Positive Regulation of Glutamate Biosynthesis in Bacillus subtilis. Dian E. Bohannon and Abraham L. Sonenshein ................................ Isolation, Nucleotide Sequence, and Preliminary Characterization of the Escherichia coli K-12 hemA Gene. Elizabeth Verkamp and Barry K. Chelm..... Purification and Properties of Ferredoxin and Rubredoxin from Butyribacterium methylotrophicum. Kazuhiko Saeki, Mahendra K. Jain, Gwo-Jenn Shen, Roger C. Prince, and J. Gregory Zeikus ................................ Overlap between pdxA and ksgA in the Complex pdxA-ksgA-apaG-apaH Operon of Escherichia coli K-12. Benjamin B. Roa, Dennis M. Connolly, and Malcolm E. Winkler................................................ An Escherichia coli Mutant Resistant to Phleomycin, Bleomycin, and Heat Inactivation Is Defective in Ubiquinone Synthesis. Christina M. Collis and Geoffrey W. Grigg ................................................... MalI, a Novel Protein Involved in Regulation of the Maltose System of Escherichia coli, Is Highly Homologous to the Repressor Proteins GaIR, CytR, and Lacd. Joachim Reidl, Karin Romisch, Michael Ehrmann, and Winfried Boos ............................................................... Transcription of pfl Is Regulated by Anaerobiosis, Catabolite Repression, Pyruvate, and oxrA: pfl::Mu dA Operon Fusions of Salmonella typhimurium. K. K. Wong, K. L. Suen, and H. S. Kwan............................. Regulation of the Escherichia coli glyA Gene by the metR Gene Product and Homocysteine. Michael D. Plamann and George V. Stauffer ............. Dual Mechanism for Stimulation of Glutamate Transport by Potassium Ions in Streptococcus mutans. Yutaka Sato, Sumihare Noji, Ryuta Suzuki, and Shigehiko Taniguchi................................................ Isolation and Sequence of ctaA, a Gene Required for Cytochrome aa3 Biosynthesis and Sporulation in Bacillus subtilis. John P. Mueller and Harry W. Taber Structure and Expression of the Cytochrome aa3 Regulatory Gene ctaA of Bacillus subtilis. John P. Mueller and Harry W. Taber....................... Analysis of the Transcriptional Unit Encoding the Genes for Rubredoxin (rub) and a Putative Rubredoxin Oxidoreductase (rbo) in Desulfovibrio vulgaris Hildenborough. Michael J. Brumlik and Gerrit Voordouw...............

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Characterization of Five Genes in the Upper-Pathway Operon of TOL Plasmid pWWO from Pseudomonas putida and Identification of the Gene Products. Shigeaki Harayama, Monique Rekik, Marcel Wubbolts, Keith Rose, Ray A. Leppik, and Kenneth N. Timmis ................................... Isolation, Characterization, and Complementation of Rhizobium meliloti 104A14 Mutants That Lack Glutamine Synthetase II Activity. John E. Somerville, Robert G. Shatters, and Michael L. Kahn .............................. Regulation of Glutamine Synthetase II Activity in Rhizobium meliloti 104A14. Robert G. Shatters, John E. Somerville, and Michael L. Kahn............ L-Serine Degradation in Escherichia coli K-12: Cloning and Sequencing of the sdaA Gene. Hongsheng Su, B. F. Lang, and E. B. Newman............. Plasmid Control of the Pseudomonas aeruginosa and Pseudomonas putida Phenotypes and of Linalool and p-Cymene Oxidation. Marie-Jose de Smet, Mark B. Friedman, and Irwin C. Gunsalus ............................. Comparative Analysis of Proteins Induced by Heat Shock, Salinity, and Osmotic Stress in the Nitrogen-Fixing Cyanobacterium Anabaena sp. Strain L-31. Arvind A. Bhagwat and Shree Kumar Apte............................. L-Arabinose Metabolism in Herbaspirillum seropedicae. Alvaro L. Mathias, Liu Un Rigo, Shigehiro Funayama, and Fabio 0. Pedrosa.................... Reversible ADP-Ribosylation of Dinitrogenase Reductase in a NifD- Mutant of Rhodospirillum rubrum. Paul W. Ludden, Lori Lehman, and Gary P. Roberts .............................................................

ENZYMES AND PROTEINS Adenylate Cyclase and Cyclic AMP Phosphodiesterase in Bradyrhizobium japonicum Bacteroids. Carol A. Catanese, David W. Emerich, and Warren L. Zahler .............................................................. Component A3 of the Methylcoenzyme M Methylreductase System of Methanobacterium thermoautotrophicum AH: Resolution into Two Components. Pierre E. Rouviere and Ralph S. Wolfe................................. The Hydrophobic Domain of Cytochrome b5 Is Capable of Anchoring ,1-Galactosidase in Escherichia coli Membranes. Sathish K. George, Lydia Najera, Ramona P. Sandoval, Cari Countryman, Ronald W. Davis, and Garret M. Ihler. A Methyl-Accepting Protein Is Involved in Benzoate Taxis in Pseudomonas putida. Caroline S. Harwood........................................ Purification and Properties of Myxococcus xanthus Cell Surface Antigen 1604. Bruce W. Jarvis and Martin Dworkin .................................. Identification and Characterization of a Cyanate Permease in Escherichia coli K-12. Young-chul Sung and James A. Fuchs........................... Cloning, Nucleotide Sequence, and Expression in Escherichia coli of the Bacillus stearothermophilus Peroxidase Gene (perA). Suvit Loprasert, Seiji Negoro, and Hirosuke Okada ................................................. Cloning, Nucleotide Sequence, and Hybridization Studies of the Type IIb HeatLabile Enterotoxin Gene of Escherichia coli. Carol L. Pickett, Edda M. Twiddy, Christopher Coker, and Randall K. Holmes..................... Heterologous Expression of the ac-Amylase Inhibitor Gene Cloned from an Amplified Genomic Sequence of Streptomyces tendae. Klaus-Peter Koller and Gunther Riess .......................................................

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Isolation, Characterization, and Biological Activity of Ferredoxin-NAD+ Reductase from the Methane Oxidizer Methylosinus trichosporium OB3b. Yung-Pin Chen and Duane C. Yoch.................................... Legionella micdadei Protein Kinase Catalyzes Phosphorylation of Tubulin and Phosphatidylinositol. Asish K. Saha, John N. Dowling, Nishit K. Mukhopadhyay, and Robert H. Glew .................................. Glycogen-Bound Polyphosphate Kinase from the Archaebacterium Sulfolobus acidocaldarius. Romuald Sk6rko, Jerzy Osipiuk, and Karl 0. Stetter...... DNA Sequence of the Escherichia coli K-12 y-Glutamyltranspeptidase Gene, ggt. Hideyuki Suzuki, Hidehiko Kumagai, Takashi Echigo, and Tatsurokuro Tochikura ........................................................... Cloning and Expression of the Alkaline Proteinase Gene from Pseudomonas aeruginosa IFO 3455. Yusaku Atsumi, Shunji Yamamoto, Kazuyuki Morihara, Jun Fukushima, Hiroaki Takeuchi, Nobuyuki Mizuki, Susumu Kawamoto, and Kenji Okuda .............................pepM Is an Essential Gene in SalmoneUa typhimurium. Charles G. Miller, A. M. Kukral, J. L. Miller, and N. Rao Movva ............................... Molecular Cloning of the Structural Gene for Alkaline Elastase YaB, a New Subtilisin Produced by an Alkalophilic Bacillus Strain. Ryuta Kaneko, Nobuto Koyama, Ying-Chieh Tsai, Ray-Yeng Juang, Koji Yoda, and Makari

Yamasaki.

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BACTERIOPHAGES Genetic Analysis of Bacteriophage N4 Adsorption. Diane R. Kiino and Lucia B. .................................................. Rothman-Denes

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POPULATION GENETICS AND EVOLUTION Comparative Sequence Analysis of a Genus-Common Rickettsial Antigen Gene. Burt E. Anderson and Theodore Tzianabos............................

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ERRATUM Reconstitution and Properties of a Coenzyme F420-Mediated Formate Hydrogenlyase System in Methanobacterium formicicum. Stephen F. Baron and James G. Ferry......................................................

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Date of Issue: 28 August 1989