Todesco 2010b SOM second resubmission final - PLOS

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SOM for MicroRNA target mimics in A. thaliana. Supplementary Table 1. Oligonucleotide primers used to modify the IPS1 sequence in MIM constructs. Construct.
Todesco et al.:

SOM for MicroRNA target mimics in A. thaliana

Supplementary Table 1. Oligonucleotide primers used to modify the IPS1 sequence in MIM constructs. Construct MIM156 MIM157 MIM158 MIM159 MIM160 MIM161a.1 MIM161a.2 MIM162 MIM163 MIM164 MIM165/166 MIM167 MIM168 MIM169 MIM169defg MIM170 MIM171a MIM171bc

Primer

Sequence (5´- 3´)

I

ctTGACAGAAGATAGAAGTGAGCATtttctagagggagataa

II

aaATGCTCACTTCTATCTTCTGTCAagcttcggttcccctcg

I

gaaccgaagctTTGACAGAAGTCGATAGAGAGCACtttctagagg

II

cctctagaaaGTGCTCTCTATCGACTTCTGTCAAagcttcggttc

I

ctTCGATAAACCAGATCTGCATCCAgtttctagagggagataa

II

aaTGCTTTGTCTCAAACATTTGGGAagcttcggttcccctcg

I

gaaccgaagctTTTGGATTGATTGAGGGAGCTCTTtttctagagg

II

cctctagaaaAAGAGCTCCCTCAATCAATCCAAAagcttcggttc

I

aaTGGCATACAGTCTAGAGCCAGGCAagcttcggttcccctcg

II

ctTGCCTGGCTCTAGACTGTATGCCAtttctagagggagataa

I

gaaccgaagcTTGAAAGTGACGAACACATCGGGGttttctagagg

II

cctctagaaaaCCCCGATGTGTTCGTCACTTTCAAgcttcggttc

I

gaaccgaagctTCAATGCATTTCTAAAAGTGACTAtttctagagg

II

cctctagaaaTAGTCACTTTTAGAAATGCATTGAagcttcggttc

I

ctTCGATAAACCAGATCTGCATCCAGtttctagagggagataa

II

aaCTGGATGCAGATCTGGTTTATCGAagcttcggttcccctcg

I

ctTTGAAGAGGATTGATTGGAACTTCGATtttctagagggagataa

II

aaATCGAAGTTCCAATCAATCCTCTTCAAagcttcggttcccctcg

I

ctTGGAGAAGCATAGGGGCACGTGCAtttctagagggagataa

II

aaTGCACGTGCCCCTATGCTTCTCCAagcttcggttcccctcg

I

ctTCGGACCAGGTAGATTCATTCCCCtttctagagggagataa

II

aaGGGGAATGAATCTACCTGGTCCGAagcttcggttcccctcg

I

gaaccgaagctTGAAGCTGCCTTGAGCATGATCTAtttctagagg

II

ctctagaaaTAGATCATGCTCAAGGCAGCTTCAagcttcggttc

I

ctTCGCTTGGTGTTTAAGGTCGGGAAtttctagagggagataa

II

aaTTCCCGACCTTAAACACCAAGCGAagcttcggttcccctcg

I

ctTAGCCAAGGAGCACGACTTGCCGAtttctagagggagataa

II

aaTCGGCAAGTCGTGCTCCTTGGCTAagcttcggttcccctcg

I

ctTGAGCCAAGGTAGGTGACTTGCCGtttctagagggagataa

II

aaCGGCAAGTCACCTACCTTGGCTCAagcttcggttcccctcg

I

ctTGATTGAGCCTTTATGTCAATATCtttctagagggagataa

II

aaGATATTGACATAAAGGCTCAATCAagcttcggttcccctcg

I

ctTGATTGAGCCTTTACGCCAATATCtttctagagggagataa

II

aaGATATTGGCGTAAAGGCTCAATCAagcttcggttcccctcg

I

ctGTTAGCCGTGTTTACAATATCACGtttctagagggagataa

II

aaCGTGATATTGTAAACACGGCTAACagcttcggttcccctcg

S1

Todesco et al.:

MIM172 MIM172cs MIM172ns MIM173 MIM319 MIM390 MIM391 MIM393 MIM394 MIM395 MIM396 MIM397 MIM398 MIM400 MIM401 MIM402 MIM403 MIM404 MIM405 MIM406 MIM407

SOM for MicroRNA target mimics in A. thaliana

I

ctAGAATCTTGAACTCGATGCTGCATtttctagagggagataa

II

aaATGCAGCATCGAGTTCAAGATTCTagcttcggttcccctcg

I

ctAGAATCTTGATAGAGATGCAGCTCtttctagagggagataa

II

aaGAGCTGCATCTCTATCAAGATTCTagcttcggttcccctcg

I

ccgaagctAGAATCTTGAGGATGCTGCATtttctagagg

II

ctagaaaTGCAGCATCCTCAAGATTCTagcttcggttc

I

gaaccgaagctTTCGCTTGCATTCGAGAGAAATCACtttctagagg

II

cctctagaaaGTGATTTCTCTCGAATGCAAGCGAAagcttcggttc

I

ctTTGGACTGAATAGAGGAGCTCCTtttctagagggagataa

II

aaAGGAGCTCCTCTATTCAGTCCAAagcttcggttcccctcg

I

ctAAGCTCAGGATAGAGGATAGCGCCtttctagagggagataa

II

aaGGCGCTATCCTCTATCCTGAGCTTagcttcggttcccctcg

I

ctTTCGCAGGAGTAGTGATAGCGCCAtttctagagggagataa

II

aaTGGCGCTATCACTACTCCTGCGAAagcttcggttcccctcg

I

ctTCCAAAGGGAGGGCCGCATTGATCCtttctagagggagataa

II

aaGGATCAATGCGGCCCTCCCTTTGGAagcttcggttcccctcg

I

ctTTGGCATTCTTAAGTCCACCTCCtttctagagggagataa

II

aaGGAGGTGGACTTAAGAATGCCAAagcttcggttcccctcg

I

ctCTGAAGTGTTAAGCGGGGAACTCtttctagagggagataa

II

aaGAGTTCCCCGCTTAACACTTCAGagcttcggttcccctcg

I

ctTTCCACAGCTAAGCTCTTGAACTGtttctagagggagataa

II

aaCAGTTCAAGAGCTTAGCTGTGGAAagcttcggttcccctcg

I

ctTCATTGAGTGTAAAATCGTTGATGtttctagagggagataa

II

aaCATCAACGATTTTACACTCAATGAagcttcggttcccctcg

I

ctTGTGTTCTCATTGAGTCACCCTGtttctagagggagataa

II

aaCAGGGTGACTCAATGAGAACACAagcttcggttcccctcg

I

gaaccgaagctTATGAGAGTAGAACTATAAGACActttctagagg

II

cctctagaaaGTGTCTTATAGTTCTACTCTCATAagcttcggttc

I

ctCGAAACTGGTTAGATCGACCGACAtttctagagggagataa

II

aaTGTCGGTCGATCTAACCAGTTTCGagcttcggttcccctcg

I

ctTTCGAGGCCTGGGATTAAACCTCTGtttctagagggagataa

II

aaCAGAGGTTTAATCCCAGGCCTCGAAagcttcggttcccctcg

I

ctTTAGATTCACTTGACACAAACTCGtttctagagggagataa

II

aaCGAGTTTGTGTCAAGTGAATCTAAagcttcggttcccctcg

I

ctATTAACGCTGTTCACGGTTGCGGCAGCtttctagagggagataa

II

aaGCTGCCGCAACCGTGAACAGCGTTAATagcttcggttcccctcg

I

ctATGAGTTGGGAACCCTAACCCATAACTtttctagagggagataa

II

aaAGTTATGGGTTAGGGTTCCCAACTCATagcttcggttcccctcg

I

ctTAGAATGCTAGAGCTGTAATCCAGtttctagagggagataa

II

aaCTGGATTACAGCTCTAGCATTCTAagcttcggttcccctcg

I

ctTTTAAATCATGGGGTACTTTTGGTtttctagagggagataa

S2

Todesco et al.:

MIM408 MIM413 MIM414 MIM415 MIM416 MIM417 MIM418 MIM419 MIM420 MIM426 MIM427 MIM447 MIM771 MIM772 MIM773 MIM774 MIM775 MIM776 MIM777 MIM778

SOM for MicroRNA target mimics in A. thaliana

II

aaACCAAAAGTACCCCATGATTTAAAagcttcggttcccctcg

I

ctATGCACTGCCAAGCCTTCCCTGGCtttctagagggagataa

II

aaGCCAGGGAAGGCTTGGCAGTGCATagcttcggttcccctcg

I

ctATAGTTTCTCAAGCTGTTCTGCACtttctagagggagataa

II

aaGTGCAGAACAGCTTGAGAAACTATagcttcggttcccctcg

I

ctTCATCTTCATTAGAATCATCGTCAtttctagagggagataa

II

aaTGACGATGATTCTAATGAAGATGAagcttcggttcccctcg

I

ctAACAGAGCAGTTCCAACAGAACATtttctagagggagataa

II

aaATGTTCTGTTGGAACTGCTCTGTTagcttcggttcccctcg

I

ctGGTTCGTACGAAGAACACTGTTCAtttctagagggagataa

II

aaTGAACAGTGTTCTTCGTACGAACCagcttcggttcccctcg

I

ctGAAGGTAGTGTATAATTTGTTCGAtttctagagggagataa

II

aaTCGAACAAATTATACACTACCTTCagcttcggttcccctcg

I

ctTAATGTGATGTTTATGAACTGACCtttctagagggagataa

II

aaGGTCAGTTCATAAACATCACATTAagcttcggttcccctcg

I

ctTTATGAAGGCAAGAGAGGATGTTGtttctagagggagataa

II

aaCAACATCCTCTCTTGCCTTCATAAagcttcggttcccctcg

I

ctTAAACTAATCTTGGCGGAAATGCAtttctagagggagataa

II

aaTGCATTTCCGCCAAGATTAGTTTAagcttcggttcccctcg

I

ctTTTTGGAAATAAGAGGTCCTTACGtttctagagggagataa

II

aaCGTAAGGACCTCTTATTTCCAAAAagcttcggttcccctcg

I

ctTTTTTCCTACAAGACCGCCCATACCtttctagagggagataa

II

aaGGTATGGGCGGTCTTGTAGGAAAAAagcttcggttcccctcg

I

ctTTGGGGACGACTTAATGTTTTGTTGtttctagagggagataa

II

aaCAACAAAACATTAAGTCGTCCCCAAagcttcggttcccctcg

I

ctTGAGCCTCTGAAACGGTAGCCCTCtttctagagggagataa

II

aaGAGGGCTACCGTTTCAGAGGCTCAagcttcggttcccctcg

I

ctTTTTTCCTACATGACCGCCCATACtttctagagggagataa

II

aaGTATGGGCGGTCATGTAGGAAAAAagcttcggttcccctcg

I

ctTTTGCTTCCATAGACTTTTGTCTCtttctagagggagataa

II

aaGAGACAAAAGTCTATGGAAGCAAAagcttcggttcccctcg

I

ctTTGGTTACCCTAGTTATGGCCATCtttctagagggagataa

II

aaGATGGCCATAACTAGGGTAACCAAagcttcggttcccctcg

I

ctTTCGATGTCTTCGTGCAGTGCCAAtttctagagggagataa

II

aaTTGGCACTGCACGAAGACATCGAAagcttcggttcccctcg

I

ctTCTAAGTCTTTAGATATTGATGTTtttctagagggagataa

II

aaAACATCAATATCTAAAGACTTAGAagcttcggttcccctcg

I

ctTACGCATTGATAGATTTCGTTGCTtttctagagggagataa

II

aaAGCAACGAAATCTATCAATGCGTAagcttcggttcccctcg

I

ctTGGCTTGGTTATGAATGTACACCGtttctagagggagataa

II

aaCGGTGTACATTCATAACCAAGCCAagcttcggttcccctcg

S3

Todesco et al.:

MIM779 MIM780 MIM781 MIM782 MIM783 MIM823 MIM824 MIM828 MIM834 MIM838 MIM841 MIM847 MIM858 MIM859 MIMB MIMH

SOM for MicroRNA target mimics in A. thaliana

I

ctTTCTGCTATGATGATGCTGCTCATtttctagagggagataa

II

aaATGAGCAGCATCATCATAGCAGAAagcttcggttcccctcg

I

ctTTTCTTCGTGTAGTATATCTGGCAtttctagagggagataa

II

aaTGCCAGATATACTACACGAAGAAAagcttcggttcccctcg

I

ctTTAGAGTTTTTAGATGGATACTTAtttctagagggagataa

II

aaTAAGTATCCATCTAAAAACTCTAAagcttcggttcccctcg

I

ctACAAACACCTATGAGGATGTTCTTtttctagagggagataa

II

aaAAGAACATCCTCATAGGTGTTTGTagcttcggttcccctcg

I

ctAAGCTTTGCTTAGAGTTCATGTTCtttctagagggagataa

II

aaGAACATGAACTCTAAGCAAAGCTTagcttcggttcccctcg

I

gaaccgaagctTGGGTGGTGAGGGTCATATAAGATtttctagagg

II

cctctagaaaATCTTATATGACCCTCACCACCCAagcttcggttc

I

gaaccgaagctTAGACCATTTAAAGTGAGAAGGGAtttctagagg

II

cctctagaaaTCCCTTCTCACTTTAAATGGTCTAagcttcggttc

I

gaaccgaagctTCTTGCTTAATTGATGAGTATTCCAtttctagagg

II

cctctagaaaTGGAATACTCATCAATTAAGCAAGAagcttcggttc

I

gaaccgaagctTGGTAGCAGTGGGAGCGGTGGTAAtttctagagg

II

cctctagaaaTTACCACCGCTCCCACTGCTACCAagcttcggttc

I

gaaccgaagctTTTTCTTCTATTGCTTCTTGCACAtttctagagg

II

cctctagaaaTGTGCAAGAAGCAATAGAAGAAAAagcttcggttc

I

gaaccgaagctTACGAGCCACAAGTTGAAACTGAAtttctagagg

II

cctctagaaaTTCAGTTTCAACTTGTGGCTCGTAagcttcggttc

I

gaaccgaagctTCACTCCTCTAAGTCTTCTTGATGtttctagagg

II

cctctagaaaCATCAAGAAGACTTAGAGGAGTGAagcttcggttc

I

gaaccgaagctTTCGTTGTCTAAAGTTCGACCTTgtttctagagg

II

cctctagaaaCAAGGTCGAACTTTAGACAACGAAagcttcggttc

I

gaaccgaagctTCGAACTCATAAGCTTTGGTGTCtttctagagg

II

cctctagaaaGACACCAAAGCTTATGAGTTCGAagcttcggttc

I

gaaccgaagctTTAGTAACAGATTCATCTTTGATTGGtttctagagg

II

cctctagaaaCCAATCAAAGATGAATCTGTTACTAAagcttcggttc

I

gaaccgaagctTCTCTCTGTTAAAGTGAAGTCAAatttctagagg

II

cctctagaaaTTTGACTTCACTTTAACAGAGAGAagcttcggttc

Nucleotides complementary to the targeted miRNA are shown in upper case. MIMB and MIMH target the putative miRNAs candidates B and H, as described in [33].

S4