Type of the Paper (Article - MDPI

Table S1. List of virulence genes tested in this study. Functional Category

Adhesins

Virulence Gene fimH papA sfaS escV bfpB fyuA

Iron acquisition

iutA iroN ireA

Protectins

kpsMT II: -K1 -K2 -K5 kpsMT III ompT traT iss

Toxins

E. coli Pathotype

Gene Function type 1 fimbriae P fimbriae S fimbriae a conservative component of the type III secretion apparatus bundle forming pilus yersiniabactin siderophore receptor aerobactin siderophore receptor salmochelin siderophore receptor iron-regulated element, siderophore receptor group II capsule group II capsule K1 variant group II capsule K2 variant group II capsule K5 variant group III capsule outer membrane protein, protease serum resistance-associated outer membrane protein increased serum survival

cnf1

cytotoxic necrotizing factor 1

hlyA east1

alpha hemolysin heat stable enterotoxin 1

ehxA

enterohemolysin

stx1 stx2 eltA estI estII agn43: Biofilm formation

-a -b -K12

Shiga toxin 1 Shiga toxin 2 heat labile toxin heat stable toxin a heat stable toxin b antigen 43 antigen 43 allele a antigen 43 allele b antigen 43 allele K12

ExPEC ExPEC ExPEC EPEC, EHEC EPEC

References to Papers which Report the PCR Primers and Conditions 16 16 16 30 30

ExPEC

16

ExPEC ExPEC

16 16

ExPEC

16

ExPEC ExPEC ExPEC ExPEC ExPEC

16 16 32 16 16

ExPEC

33

ExPEC

16

ExPEC ExPEC, NTEC ExPEC EAEC EPEC, EHEC EHEC EHEC ETEC ETEC ETEC ExPEC ExPEC ExPEC ExPEC

16 34 34 35 35 30 30 35 35 35 36 36 36 36

Table S2. Prevalence of the resistance genes among the antimicrobial-resistant E. coli isolates from healthy adults and young children.

Antimicrobial Agent

Ampicillin

Resistance Gene

blaTEM blaSHV

Cefotaxim blaCTX-M Streptomycin

Tetracycline

strA/strB aadA1 tetA tetB tetC

Int. J. Environ. Res. Public Health 2018, 15, x; doi:

Number (%) of Isolates with Resistance Genes

Test of Independence p-Value

Adults n = 113 54 (47.8) 6 (5.3) n=4 4 (100) n = 137 8 (5.8) 12 (8.8) n = 63 15 (23.8) 32 (50.8) 4 (6.3)

Young Children n = 27 13 (48.1) 3 (11.1) n=2 2 (100) n = 19 3 (15.8) 4 (21.1) n = 17 4 (23.5) 7 (41.2) 0

0.97312 0.3749 1 0.1343 0.1099 1 0.6668 0.5732

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Int. J. Environ. Res. Public Health 2018, 15, x

Trimethoprim/ Sulfamethoxazol

Nalidixic acid

dfrA1 dfrA7 sul1 sul2 sul3 qnrA qnrB qnrS

2 of 2 n = 33 8 (24.2) 18 (54.5) 5 (15.2) 20 (60.6) 0 n = 39 0 2 (5.1) 7 (17.9)

n = 12 1 (8.3) 4 (33.3) 2 (16.7) 3 (25) 0 n=8 0 1 (12.5) 2 (25)

Figure S1. Age distribution among adults.

0.4069 0.8494 1 0.0758 0.4364 0.6388